Sperm biological characteristics improves when sperm preparation is done before cryopreservation in varicocele patients.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-05-01

M Vasiee, A Agharahimi, M A Khalili, A Rahavian, N Aboutaleb

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引用次数: 0

Abstract

Background: Sperm cryopreservation is a process that involves preserving sperm cells for future use. Prior to freezing, sperm preparation methods like density gradient centrifugation (DGC) are typically performed to improve sperm quality.

Objective: To compare two sperm freezing methods, with and without seminal fluid, in normal and varicocele patients, focusing on reactive oxygen species (ROS) levels and sperm DNA fragmentation.

Materials and methods: Forty samples from individuals with normal (n=20) and varicocele (n=20) were collected through ejaculation. Each sample was divided into two groups for analysis. One group DGC was performed pre-freezing and another group DGC was done post-freezing. Evaluations were conducted on sperm parameters, DNA fragmentation index, and ROS generation.

Results: In the normal group, progressive motility in Fresh Freeze DGC (19.55 ± 12.90) was lower than Fresh DGC Freeze (32.45 ± 12.86), although viability, morphology, DNA fragmentation and ROS production remained unchanged. Fresh Freeze DGC (44.25 ± 11.38) and Fresh DGC Freeze (53.58 ± 11.41) differed substantially in the varicocele group for viability assessment, whereas other parameters did not change in this group.

Conclusion: It is recommended to prepare sperm before freezing to avoid potential issues with sperm not tolerating the centrifugation process. Removing seminal fluid before freezing can lead to better sperm parameters in both normal and varicocele groups. Doi.org/10.54680/fr25310110712.

本刊更多论文

精索静脉曲张患者在冷冻保存前进行精子准备可改善精子生物学特性。

背景：精子冷冻保存是一种保存精子细胞以备将来使用的过程。在冷冻之前，通常会采用密度梯度离心（DGC）等精子制备方法来提高精子质量：比较正常精子和精索静脉曲张患者的两种精子冷冻方法（有精液和无精液），重点关注活性氧（ROS）水平和精子 DNA 片段：通过射精采集正常（20 人）和精索静脉曲张（20 人）患者的 40 份样本。每个样本分为两组进行分析。一组在冷冻前进行 DGC 分析，另一组在冷冻后进行 DGC 分析。对精子参数、DNA碎片指数和ROS生成进行评估：在正常组中，新鲜冷冻 DGC 的精子运动能力（19.55 ± 12.90）低于新鲜冷冻 DGC 的精子运动能力（32.45 ± 12.86），但存活率、形态、DNA 碎片和 ROS 产生量保持不变。新鲜冷冻DGC（44.25 ± 11.38）和新鲜冷冻DGC（53.58 ± 11.41）在精索静脉曲张组的存活率评估中差异很大，而该组的其他参数没有变化：建议在冷冻前对精子进行准备，以避免精子无法承受离心过程的潜在问题。结论：建议在冷冻前对精子进行准备，以避免精子不耐受离心过程的潜在问题。在冷冻前去除精液可使正常组和精索静脉曲张组的精子参数得到改善。Doi.org/10.54680/fr25310110712.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.