Protective effects of hydrogen (H₂) on boar sperm upon freeze-thaw process.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-05-01

S Zhang, S Ren, C Wu, J Dai, Y Zhang, D Zhang

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引用次数: 0

Abstract

Background: Antioxidant is important to prevent ROS attack in boar sperm during the freezing-thawing process.

Objective: To study whether hydrogen (H₂) could improve the survival of boar sperm upon the freeze-thaw process.

Materials and methods: The effects of hydrogen in pre-diluent, freezing extender and thawing solution were investigated. Sperm viability, progressive motility, acrosomal integrity, mitochondrial activity, as well as the levels of malonaldehyde (MDA) and total antioxidant capacity (T-AOC) were measured.

Results: Hydrogen in pre-diluent, freezing extender and thawing solution significantly improved sperm quality parameters (including sperm viability, progressive motility, acrosomal integrity and mitochondrial activity), increased total antioxidant capacity (T-AOC) and decreased malondialdehyde (MDA) of post-thawed boar sperm. The use of hydrogen in all three solutions improved the quality of post-thawed boar sperm.

Conclusion: Hydrogen protects boar sperm against ROS-induced damages during cooling and the freezing-thawing process. Doi.org/10.54680/fr25310110512.

本刊更多论文

氢对猪精子冻融过程的保护作用。

背景：在猪精子冻融过程中，抗氧化剂对防止ROS的攻击起着重要作用。目的：研究氢（H2）是否能提高猪精子在冻融过程中的存活率。材料与方法：考察了预稀释剂、冷冻扩展剂和解冻液中氢的影响。测定精子活力、渐进式活力、顶体完整性、线粒体活性以及丙二醛（MDA）和总抗氧化能力（T-AOC）水平。结果：氢在预稀释剂、冷冻扩展剂和解冻液中显著改善了猪精子的质量参数（包括精子活力、进行性、顶体完整性和线粒体活性），提高了解冻后猪精子的总抗氧化能力（T-AOC），降低了丙二醛（MDA）。在所有三种溶液中使用氢都提高了解冻后野猪精子的质量。结论：在冷却和冻融过程中，氢对猪精子有保护作用。Doi.org/10.54680/fr25310110512。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.