Advancements in cryopreservation techniques for human gametes and embryos: novel cryoprotectants and their influence on fertility preservation.

IF 0.9 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2025-07-01
A S Vickram, C Prasanth, S Bharath, D Jenila Rani, C M Mathan Muthu, B Bhavani Sowndharya
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引用次数: 0

Abstract

Background: Cryopreservation has transformed fertility preservation by enabling the long-term storage of gametes and embryos. Although traditional procedures such as slow freezing and vitrification are routinely used, recent innovations and new cryoprotectants have had a significant impact on reproductive medicine outcomes.

Objective: This review examines advances in cryopreservation techniques, the influence of novel cryoprotectants, and the implications for gamete and embryo survival, viability, and clinical outcomes in fertility preservation.

Materials and methods: We conducted a comprehensive review of the existing literature on classic and novel cryopreservation methods for sperm, oocytes, and embryos. Advances in vitrification methods, the invention of novel cryoprotectants, and comparative effectiveness and toxicity assessments were all evaluated. Clinical data on survival rates, implantation rates, and fertility preservation were thoroughly reviewed.

Results: Improvements in vitrification procedures have drastically enhanced oocyte survival and developmental potential, resolving some of the previously linked cryopreservation issues. Innovative ways to cryopreserve have enhanced sperm survival and motility after thawing. The focus of embryo cryopreservation has switched from traditional slow freezing to precise vitrification, resulting in higher survival rates and better clinical results. Novel cryoprotectants have shown promise in terms of reduced toxicity and improved cryosurvival while retaining biological integrity. Overall, these advances have had a positive impact on fertility preservation techniques and clinical success rates.

Conclusion: Emerging cryopreservation methods, such as breakthroughs in vitrification protocols and the identification of new cryoprotectants, have significantly improved gamete and embryo storage efficiency. Such developments not only increase the longevity and quality of cryopreserved materials, but they also improve therapeutic outcomes in fertility preservation. Additional study and optimization are required to standardize these procedures for optimal use in a variety of patient populations. https://doi.org/10.54680/fr25410110112.

人类配子和胚胎冷冻保存技术的进展:新型冷冻保护剂及其对生育能力保存的影响。
背景:低温保存通过使配子和胚胎能够长期保存,改变了生育能力的保存。虽然缓慢冷冻和玻璃化冷冻等传统方法是常规使用的,但最近的创新和新的冷冻保护剂对生殖医学结果产生了重大影响。目的:本文综述了冷冻保存技术的进展,新型冷冻保护剂的影响,以及对配子和胚胎存活、活力和生育保存临床结果的影响。材料和方法:我们对现有文献中关于精子、卵母细胞和胚胎的经典和新型冷冻保存方法进行了全面的综述。玻璃化方法的进展,新型冷冻保护剂的发明,以及比较有效性和毒性评估都进行了评估。临床资料的存活率,着床率,和生育能力保存进行了全面审查。结果:玻璃化方法的改进大大提高了卵母细胞的存活率和发育潜力,解决了一些以前与冷冻保存有关的问题。创新的冷冻保存方法提高了精子在解冻后的存活率和活力。胚胎低温保存的重点已从传统的慢速冷冻转向精确的玻璃化冷冻,从而提高了胚胎的存活率和临床效果。新型低温保护剂在降低毒性和提高低温存活率同时保持生物完整性方面显示出前景。总的来说,这些进步对生育保存技术和临床成功率产生了积极的影响。结论:新出现的冷冻保存方法,如玻璃化技术的突破和新型冷冻保护剂的鉴定,显著提高了配子和胚胎的保存效率。这些发展不仅增加了冷冻保存材料的寿命和质量,而且还改善了生育保存的治疗结果。需要进一步的研究和优化来标准化这些程序,以便在各种患者群体中最佳地使用。https://doi.org/10.54680/fr25410110112。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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