Procedia Technology最新文献_第4页

Highly-sensitive Label-free Differential Pulse Voltammetric Immunosensor for Diagnosis of Infectious Diseases Based on Electrospun Copper Doped ZnO Nanofiber Biosensing Platform 基于电纺铜掺杂ZnO纳米纤维生物传感平台的高灵敏度无标记差分脉冲伏安免疫传感器

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.092

K. Brince Paul , Sanni Kumar , Suryasnata Tripaty , Vikrant Singh , Siva Rama Krishna Vanjari , Shiv Govind Singh

{"title":"Highly-sensitive Label-free Differential Pulse Voltammetric Immunosensor for Diagnosis of Infectious Diseases Based on Electrospun Copper Doped ZnO Nanofiber Biosensing Platform","authors":"K. Brince Paul , Sanni Kumar , Suryasnata Tripaty , Vikrant Singh , Siva Rama Krishna Vanjari , Shiv Govind Singh","doi":"10.1016/j.protcy.2017.04.092","DOIUrl":"10.1016/j.protcy.2017.04.092","url":null,"abstract":"<div><p>Rapid detection of infectious diseases has generated significant interest in recent years. The time consuming and costly conventional diagnostics methods substantiate the need to develop a cost-effective rapid infectious disease detection platform to address the persistently threatening health issues in developing countries. The recent advancements in nanotechnology and biosensing have manifested the potential to deliver an effective point-of-care diagnostics platform. In this work, the synthesis and fabrication of an ultrasensitive Copper doped Zinc oxide nanofiber based biosensing platform is reported. Copper doped Zinc oxide nanofibers are synthesized by simple electrospinning technique with fiber diameter of 100-200nm. The structural and morphological characteristics of the nanofibres are studied using X-ray diffraction and field emission scanning electron microscopy. The label free detection of HRP2 protein with the Copper doped Zinc Oxide nanofiber has been investigated by Differential pulse voltammetric technique. Mercaptopropionic acid treatment of Copper doped Zinc oxide nanofiber generates carboxylic acid groups, which facilitate the covalent conjugation of Anti-HRP2. To the best of our knowledge, the fabricated immunosensor displays better sensitivity than the best malaria sensor reported in the literature based on different nanomaterials and different detection mechanism. The proposed platform exhibits very low limit of detection of 10 attogram per ml for the targeted HRP2 protein in a wide detection test range (ag/ml -μg/ml). The novel biosensor platform demonstrates good stability and selectivity which can be implemented for point-of-care diagnosis of biomarkers related to other infectious diseases.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 219-220"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77570613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Multiplexed Electrochemical Immunosensor for Obesity-related Hormones Using Grafted Graphene-modified Electrodes as Platforms for Antibodies Immobilization 用石墨烯修饰电极作为抗体固定平台的多路电化学肥胖相关激素免疫传感器

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.080

G. Martínez-García, L. Agüí, P. Yáñez-Sedeño, J.M. Pingarrón

引用次数: 7

Fabrication of Cost-effective and Lithographically Patterned Flexible Paper Based Microfluidic Device Using Photo-PDMS for Point of Care Application 基于光电pdms的低成本光刻图纹柔性纸微流控装置的制造

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.049

Siva Prakasam O. Kare , Debanjan Das , Koel Chaudhury , Soumen Das

引用次数: 1

Application of Amorphous Indium Gallium Zinc Oxide Thin Film Transistor Biosensors in Creatine Kinase Detection 非晶铟镓锌氧化物薄膜晶体管生物传感器在肌酸激酶检测中的应用

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.111

Hsin-Chun Lu, Y. Chueh, Ting Tseng, Chung-Yih Wang, C. Chaou

引用次数: 1

Monitoring Growth and Antibiotic Susceptibility of Escherichia coli with Photoluminescence Emitting Semiconductor Biochips 利用光致发光半导体生物芯片监测大肠杆菌生长及抗生素敏感性

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.104

Elnaz Nazemi, W. Hassen, E. Frost, J. Dubowski

引用次数: 1

High-throughput Platform for Screening Microbial Fuel Cell Components 筛选微生物燃料电池组件的高通量平台

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.112

A. Vishwanathan, K. S. Aiyer, S. Sai, G. Rao

引用次数: 1

SO2SAFE - Enzymatic SO2 Biosensor for Rapid Food Safety Monitoring SO2SAFE -用于食品安全快速监测的酶促SO2生物传感器

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.024

E. Jubete , A. Jaureguibeitia , L. Añorga , P.J. Lamas-Ardisana , G. Martínez , V. Serafín , G. Cabañero , E. Ramos , S. Salleres , H.J. Grande , A. Albizu

引用次数: 3

The Dynamics of Viscoelastic Layered Systems Studied by Surface Acoustic Wave (SAW) Sensors Operated in a Liquid Phase 用表面声波(SAW)传感器研究了在液相中工作的粘弹性层状系统的动力学

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.044

A. Vikström, M.V. Voinova

引用次数: 1

B-Type Natriuretic Peptide (BNP) Detection Using Electrochemical Immunosensor Based on Sandwich ELISA with Horseradish Peroxidase-Tetramethylbenzidine System 基于夹心ELISA的电化学免疫传感器检测辣根过氧化物酶-四甲基联苯胺体系b型利钠肽

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.065

Yeni Wahyuni Hartati , Ratna Nurmalasari , Shabarni Gaffar , Toto Subroto

{"title":"B-Type Natriuretic Peptide (BNP) Detection Using Electrochemical Immunosensor Based on Sandwich ELISA with Horseradish Peroxidase-Tetramethylbenzidine System","authors":"Yeni Wahyuni Hartati , Ratna Nurmalasari , Shabarni Gaffar , Toto Subroto","doi":"10.1016/j.protcy.2017.04.065","DOIUrl":"10.1016/j.protcy.2017.04.065","url":null,"abstract":"<div><p>A study of B-type natriuretic peptide (BNP) antigen as a good prognostic marker for patients with heart failure antigen detection employing an electrochemical immunosensor is described here. Monoclonal anti-BNP capture antibody was immobilized on streptavidin-modified SPCEs to increase the sensitivity of the assay. An anti-BNP detection antibody conjugated with horseradish peroxidase enzyme (HRP) and 3,3,5,5’-tetramethybezidine dihydrochloride (TMB) was used as a substrate, in connection with voltammetric technique was achieve by measuring the peak current. Incorporation of a streptavidin/biotin system resulted in a well-oriented antibody immobilization of the capture antibody and consequently enhanced the sensitivity of the assay. Several optimizations were performed to reduce background signals; the optimal concentration of anti-BNP detection antibody needed, the time of SPCEs were first blocked with glycine, incubated with various concentrations of BNP and HRP-conjugated anti-BNP detection antibody (0 to 100.0 ng/mL), followed by electrochemical measurement were investigated,. In conclusion, this immunosensor greatly shortened and convenient for detection of heart failure diagnosis in real serum samples.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 149-150"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77117982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Development of Electrochemical Glucose Biosensor for the Estimation of Cancer Cell Proliferation 电化学葡萄糖生物传感器在癌细胞增殖评价中的应用

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.095

Madhurantakam Sasya , K. Jayanth babu , John Bosco Balaguru Rayappan , Uma Maheshwari Krishnan

引用次数: 2