Procedia Technology最新文献

{"title":"Highly-sensitive Label-free Differential Pulse Voltammetric Immunosensor for Diagnosis of Infectious Diseases Based on Electrospun Copper Doped ZnO Nanofiber Biosensing Platform","authors":"K. Brince Paul ,&nbsp;Sanni Kumar ,&nbsp;Suryasnata Tripaty ,&nbsp;Vikrant Singh ,&nbsp;Siva Rama Krishna Vanjari ,&nbsp;Shiv Govind Singh","doi":"10.1016/j.protcy.2017.04.092","DOIUrl":"10.1016/j.protcy.2017.04.092","url":null,"abstract":"<div><p>Rapid detection of infectious diseases has generated significant interest in recent years. The time consuming and costly conventional diagnostics methods substantiate the need to develop a cost-effective rapid infectious disease detection platform to address the persistently threatening health issues in developing countries. The recent advancements in nanotechnology and biosensing have manifested the potential to deliver an effective point-of-care diagnostics platform. In this work, the synthesis and fabrication of an ultrasensitive Copper doped Zinc oxide nanofiber based biosensing platform is reported. Copper doped Zinc oxide nanofibers are synthesized by simple electrospinning technique with fiber diameter of 100-200<!--> <!-->nm. The structural and morphological characteristics of the nanofibres are studied using X-ray diffraction and field emission scanning electron microscopy. The label free detection of HRP2 protein with the Copper doped Zinc Oxide nanofiber has been investigated by Differential pulse voltammetric technique. Mercaptopropionic acid treatment of Copper doped Zinc oxide nanofiber generates carboxylic acid groups, which facilitate the covalent conjugation of Anti-HRP2. To the best of our knowledge, the fabricated immunosensor displays better sensitivity than the best malaria sensor reported in the literature based on different nanomaterials and different detection mechanism. The proposed platform exhibits very low limit of detection of 10 attogram per ml for the targeted HRP2 protein in a wide detection test range (ag/ml -μg/ml). The novel biosensor platform demonstrates good stability and selectivity which can be implemented for point-of-care diagnosis of biomarkers related to other infectious diseases.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 219-220"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77570613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplexed Electrochemical Immunosensor for Obesity-related Hormones Using Grafted Graphene-modified Electrodes as Platforms for Antibodies Immobilization","authors":"G. Martínez-García,&nbsp;L. Agüí,&nbsp;P. Yáñez-Sedeño,&nbsp;J.M. Pingarrón","doi":"10.1016/j.protcy.2017.04.080","DOIUrl":"10.1016/j.protcy.2017.04.080","url":null,"abstract":"<div><p>An electrochemical immunosensor for the simultaneous determination of ghrelin (GHRL) and peptide YY (PYY) using dual screen-printed carbon electrodes modified with reduced graphene oxide (rGO) is presented. Diazonium salt of 4-aminobenzoic acid (4-ABA) was electrochemically grafted on the modified electrodes allowing covalent immobilization of antibodies. After competitive immunoassays using alkaline phosphatase labelled antigens, the affinity reactions were monitored by DPV upon addition of 1-naphthyl phosphate. Calibration plots showed linear current vs. log [hormone] ranges from 10^-3 to 100 ng/mL GHRL, and 10^-4 to10 ng/mL PYY. The usefulness of dual immunosensor was demonstrated by analysis of spiked human serum and saliva.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 187-189"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87635917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Cost-effective and Lithographically Patterned Flexible Paper Based Microfluidic Device Using Photo-PDMS for Point of Care Application","authors":"Siva Prakasam O. Kare ,&nbsp;Debanjan Das ,&nbsp;Koel Chaudhury ,&nbsp;Soumen Das","doi":"10.1016/j.protcy.2017.04.049","DOIUrl":"10.1016/j.protcy.2017.04.049","url":null,"abstract":"<div><p>We have developed a lithographically patterned flexible paper-based microfluidic device using photo polydimethylsiloxane (PDMS). Normal PDMS is converted into photosensitive PDMS with the desired photoinitiator. Further paper is lithographically patterned by photo PDMS to form hydrophobic region, where the flow of aqueous solution are restricted and a hydrophilic region, where fluids can flow by capillary action without any need of an external pump. The fabricated device can attain three-dimensional structured network flow on a single paper by repeated crease, without stacking multiple layers of individual paper.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 112-113"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82053598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Amorphous Indium Gallium Zinc Oxide Thin Film Transistor Biosensors in Creatine Kinase Detection","authors":"Hsin-Chun Lu, Y. Chueh, Ting Tseng, Chung-Yih Wang, C. Chaou","doi":"10.1016/J.PROTCY.2017.04.111","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.111","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"1 1","pages":"258-259"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77661323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring Growth and Antibiotic Susceptibility of Escherichia coli with Photoluminescence Emitting Semiconductor Biochips","authors":"Elnaz Nazemi, W. Hassen, E. Frost, J. Dubowski","doi":"10.1016/J.PROTCY.2017.04.104","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.104","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"33 1","pages":"244-245"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79068896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput Platform for Screening Microbial Fuel Cell Components","authors":"A. Vishwanathan, K. S. Aiyer, S. Sai, G. Rao","doi":"10.1016/J.PROTCY.2017.04.112","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.112","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"3 1","pages":"260-262"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81866882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SO2SAFE - Enzymatic SO2 Biosensor for Rapid Food Safety Monitoring","authors":"E. Jubete ,&nbsp;A. Jaureguibeitia ,&nbsp;L. Añorga ,&nbsp;P.J. Lamas-Ardisana ,&nbsp;G. Martínez ,&nbsp;V. Serafín ,&nbsp;G. Cabañero ,&nbsp;E. Ramos ,&nbsp;S. Salleres ,&nbsp;H.J. Grande ,&nbsp;A. Albizu","doi":"10.1016/j.protcy.2017.04.024","DOIUrl":"10.1016/j.protcy.2017.04.024","url":null,"abstract":"<div><p>An amperometric sulfite biosensor was developed based on disposable screen printed electrodes (SPEs) and sulfite oxidase (SOx) enzyme. The developed biosensor shows good sensitivity (62 nA/ppm), reproducibility (RSD = 4%; n = 5) and a linear range of 15-1000<!--> <!-->ppm. The applicability of the biosensor for the analysis of sodium metabisulfite in shrimp farm samples was demonstrated successfully.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 51-52"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78305872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Dynamics of Viscoelastic Layered Systems Studied by Surface Acoustic Wave (SAW) Sensors Operated in a Liquid Phase","authors":"A. Vikström,&nbsp;M.V. Voinova","doi":"10.1016/j.protcy.2017.04.044","DOIUrl":"10.1016/j.protcy.2017.04.044","url":null,"abstract":"<div><p>We theoretically study a three-layer continuum model of a surface acoustic wave sensor where the two overlayers are allowed to be viscoelastic. This case is particularly important in biosensing, where soft materials submerged in fluids are commonplace. From the general dispersion equation, we calculate the phase velocity shift and the wave attenuation. We show that there is a viscoelastic coupling between the overlayers which results in unintuitive behavior, e.g., the addition of viscous loading to a soft-film sensor can reduce the attenuation.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 102-103"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86226982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enabling Mobile Health","authors":"Anthony P.F. Turner","doi":"10.1016/j.protcy.2017.04.003","DOIUrl":"10.1016/j.protcy.2017.04.003","url":null,"abstract":"<div><p>The need for new, easy-to-use, home and decentralised diagnostics is now greater than ever and it is rapidly becoming apparent that biosensors can contribute substantially to reducing healthcare costs. New thinking is crucial to finding effective solutions that deliver the high quality of life rightly demanded by our ever ageing population while leveraging technology to deliver this in a cost-effective manner. Several key drivers are catalysing change. Personalised medicine recognises that every individual is different and needs a tailor-made health package; these differences can only be identified with an appropriate suite of diagnostics. Individuals are increasing recognising that data about their bodies should be owned by them and that they should have the choice to use and supplement this information. This generates consumer choice and drives evidence-based payment, where the success of outcomes needs to be measured. Focus on the individual and their needs drives decentralisation and the possible radical restructuring of how we deliver health management. We already see “health rooms” in pharmacies, but the next step will be health rooms in your home, in your pocket or on your wrist. These advances are underpinned by technologies facilitating mobility and data processing, but at the core are rapid, convenient and easy ways to measure our body chemistries at the genomic, proteomic and metabolomic levels. This presentation will focus on meeting these challenges using paper-based electronics, polymers and integrated electrochemical systems to deliver inexpensive instruments for a wide range of bioanalytical applications. Approaches will be illustrated by multi-parametric monitoring for the management of diabetes, chronic kidney disease and stress, reversible and label-free affinity sensors for cancer markers and heart disease, aptasensors for pathogens and cancer cells, and robust microbial-differentiation arrays. Further development will result in cost reduction and a diversity of formats such as point-of-care tests, smart packaging, telemetric strips and print-on-demand analytical devices.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 4-5"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88617436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Electrochemical Glucose Biosensor for the Estimation of Cancer Cell Proliferation","authors":"Madhurantakam Sasya ,&nbsp;K. Jayanth babu ,&nbsp;John Bosco Balaguru Rayappan ,&nbsp;Uma Maheshwari Krishnan","doi":"10.1016/j.protcy.2017.04.095","DOIUrl":"10.1016/j.protcy.2017.04.095","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 226-228"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83600425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}