Procedia Technology最新文献

{"title":"Innovative Methods for the Integration of Immunosensors Based on Magnetic Nanoparticles in Lab-on-Chip","authors":"Olivier Lefebvre , Fabrice Mbock Nkot , Claire Smadja , Emile Martincic , Marion Woytasik , Mehdi Ammar","doi":"10.1016/j.protcy.2017.04.088","DOIUrl":"10.1016/j.protcy.2017.04.088","url":null,"abstract":"<div><p>Commonly immunoassay using magnetic nanoparticles (MNP) are performed under the control of permanent magnet close to the micro-tube of reaction<sup>1</sup>. Using a magnet gives a powerful method for driving MNP but remains unreliable or insufficient, for a fully integrated immunoassay on lab-on-chip. The aim of this study is to develop a novel lab-on-chip (Figure 1.B) for high efficient immunoassays to detect pathogenic bacteria with microcoils employed for trapping MNP during the biofunctionalization steps. Studies on bacteria are mainly based on E. Coly<sup>2,3</sup> which is a non-pathogenic bacteria and can be find everywhere. In our case we use ovalbumin which is defined as a biodefense model protein. The objectives are essentially to optimize their efficiency for biological recognition, by assuring a better bioactivity (antibodies-ovalbumin), and detect small concentrations of the targeted protein (∼10 pg/mL).</p><p>The fluidic microsystem is made of PDMS, which is micro-molded in SU8, it had channels with 50 μm height and 500 μm width. Microfluidic conditions permit a faster biofonctionnalisation step than in test tube and allow capture and detection of biological elements integrated in lab-on-chip.</p><p>Microcoils are electrodeposited on silicon using cupper. They are microfabricated with cupper wire of 10 μm height, 10 μm width, 10 μm space between wire and 45 spires. Microcoils are encapsulated in microfluidic chip by covering them with a spin-coated thin layers of PDMS. Microcoils give a local and efficient trapping of MNP and a fully integrated device.</p><p>Biological activity is studied respecting ELISA protocol with ovalbumin as protein of interest. To graft the primary antibody and protect the free area of MNP we used carboxylic as terminal group for grafting antibodies and BSA (Bovine Serum Albumin) for passivation (Figure 1.A). We characterize this method by measuring the intensity of the antibody of detection using FITC (Fluorescein isothiocynathe). Intensity is detected by fluorescent microscope connected to the microfluidic plateform and images are processed using a home-made script.</p><p>First we studied the response of immunoassays complex function of MNP size (200 nm, 300 nm and 500 nm), we confirmed that with a lower diameter we increase the intensity detected, following specific surface formula (1), (2), (3).<span><span><img></span></span></p><p>Regarding the magnetic force needed (depending of several parameters including magnetic field and parameters of the particle) and the intensity detected we selected 300 nm size of NPM.</p><p>We studied the response of immunoassays complex function of ovalbumin concentration. We realized different immunoassays by controlling MNP (Figure 1.C&D) in test tube and in microfluidic device using a magnet. The comparison between these two experiments allow us to show an improved limit of detection (<em>L.O.D. = I</em><sub><em>0</em></sub> <em>– 3 × σ</em><sub><em>D</em></su","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 210-211"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82142647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-inspired Artificial Muscle Based on Chemical Sensors","authors":"Andrea Ravalli ,&nbsp;Claudio Rossi ,&nbsp;Giovanna Marrazza","doi":"10.1016/j.protcy.2017.04.070","DOIUrl":"10.1016/j.protcy.2017.04.070","url":null,"abstract":"<div><p>In this work, we have investigated the modeling, design and fabrication of bio-inspired artificial muscle unit capable of contracting according to the directives sent in form of chemical messengers. This new technology has the potential to revolutionize current robotics, because it could permit a paradigm shift in robots: from electro-mechanical devices to electro-chemical devices. The bio-inspired artificial muscle will be based on basic contractile units coupled to electrochemical sensors, with the purpose of allowing adaptive and flexible control similar to that in animal locomotion. An artificial nerve termination, able to modify the chemical characteristics of the inner environment, will generate directives in form of chemical messengers. Electro-chemical sensors have been used in order to detect the presence of the chemical messengers and transform them into electronic signals to be used in conventional control electronics. This study has been focused on the development and optimization of sensing materials for inorganic ions such as hydrogen ions. Among various conducting polymers studied, polyaniline (PANI) has attracted much attention due to its unique and controllable chemical and electrical properties. PANI layer has been electrochemically deposited on the gold arrays surface by cyclic voltammetry. Preliminary experiments on PANI-modified sensors in order to obtain the better sensitivity as chemical sensing used in artificial muscle unit have been carried out. To allow diffusion of chemical messages, the system has been immersed in wet environment. Using this approach, we study the effective possibility to control, assessing the performance in terms of accuracy of the control of the contraction, the impact of the delay due to the transmission time of the chemicals, precision and stability of control.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 161-162"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75547613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured Platform Based on Graphene-polypyrrole Composite for Immunosensor Fabrication","authors":"Andreea Cernat,&nbsp;Mihaela Tertiș,&nbsp;Claudia Nicoleta Păpară,&nbsp;Ede Bodoki,&nbsp;Robert Săndulescu","doi":"10.1016/j.protcy.2017.04.047","DOIUrl":"10.1016/j.protcy.2017.04.047","url":null,"abstract":"<div><p>A hybrid graphene-polypyrrole (PPy) composite-based platform was elaborated by using nanosphere lithography structuration. This platform was further used for the antibody anti-acetaminophen immobilization in order to obtain an immunosensor for selective detection of paracetamol (acetaminophen) by using electrochemical methods. After patterning of the composite layer, the five-fold improvement of electrochemical signal was observed, suggesting that a higher amount of antibody was immobilized. This strategy permitted the ease and controlled immobilization of bioreceptors on the composite graphene-PPy platform and enhanced the sensitivity for paracetamol detection.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 108-109"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54987386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helix Channel Microfluidic Electrophoresis Chip Drove by Low Voltage","authors":"Jiechao Li ,&nbsp;Weiping Yan ,&nbsp;Hongfeng Lv","doi":"10.1016/j.protcy.2017.04.050","DOIUrl":"10.1016/j.protcy.2017.04.050","url":null,"abstract":"<div><p>The conventional microfluidic electrophoresis chip must be applied higher voltage, which limited the microminiaturization and integration. According to the principle of electrophoresis chip, a helix channel electrophoresis chip drove by low voltage was proposed. Some key techniques were researched, which include the bubble issue, optimization of the chip structure, low cost hydrophilic surface modification for the chip, design of miniaturized control and detection system. Test results shown the helix channel chip has better separation than the cross channel chip, and the sample can be successfully separated below 100 V voltage.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 114-115"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89871394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point of Care with Micro Fluidic Paper Based Device Incorporated with Nanocrys of Zeolite –GO for Electrochemical Sensing of Date Rape Drug","authors":"Jagriti Narang ,&nbsp;Nitesh Malhotra ,&nbsp;Chaitali Singhal ,&nbsp;Ashish Mathur ,&nbsp;Dhritiman Chakraborty ,&nbsp;Aviraj Ingle ,&nbsp;C.S. Pundir","doi":"10.1016/j.protcy.2017.04.039","DOIUrl":"10.1016/j.protcy.2017.04.039","url":null,"abstract":"<div><p>The objective of the present invention is to develop an ultrasensitive technique for the electro analysis of rape drug. A paper chip (EμPADs) was developed using nanocrystals (Nanocrys) of graphene-oxide and zeolites (Zeo-GO). Nanocrys modified EμPAD showed wide linear range 0.001 - 5 nM/ml and low detection limit of 0.00002 nM/ml. The developed sensor was tested in real time samples like alcoholic and non-alcoholic drinks and found good correlation (99%). Extensive development can be made for industrial translation of this fabricated device.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 91-93"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90244537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enabling Mobile Health","authors":"Anthony P.F. Turner","doi":"10.1016/j.protcy.2017.04.003","DOIUrl":"10.1016/j.protcy.2017.04.003","url":null,"abstract":"<div><p>The need for new, easy-to-use, home and decentralised diagnostics is now greater than ever and it is rapidly becoming apparent that biosensors can contribute substantially to reducing healthcare costs. New thinking is crucial to finding effective solutions that deliver the high quality of life rightly demanded by our ever ageing population while leveraging technology to deliver this in a cost-effective manner. Several key drivers are catalysing change. Personalised medicine recognises that every individual is different and needs a tailor-made health package; these differences can only be identified with an appropriate suite of diagnostics. Individuals are increasing recognising that data about their bodies should be owned by them and that they should have the choice to use and supplement this information. This generates consumer choice and drives evidence-based payment, where the success of outcomes needs to be measured. Focus on the individual and their needs drives decentralisation and the possible radical restructuring of how we deliver health management. We already see “health rooms” in pharmacies, but the next step will be health rooms in your home, in your pocket or on your wrist. These advances are underpinned by technologies facilitating mobility and data processing, but at the core are rapid, convenient and easy ways to measure our body chemistries at the genomic, proteomic and metabolomic levels. This presentation will focus on meeting these challenges using paper-based electronics, polymers and integrated electrochemical systems to deliver inexpensive instruments for a wide range of bioanalytical applications. Approaches will be illustrated by multi-parametric monitoring for the management of diabetes, chronic kidney disease and stress, reversible and label-free affinity sensors for cancer markers and heart disease, aptasensors for pathogens and cancer cells, and robust microbial-differentiation arrays. Further development will result in cost reduction and a diversity of formats such as point-of-care tests, smart packaging, telemetric strips and print-on-demand analytical devices.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 4-5"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88617436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene-based Biosensors for Dopamine Determination","authors":"Luminiţa Fritea ,&nbsp;Mihaela Tertiș ,&nbsp;Alan Le Goff ,&nbsp;Serge Cosnier ,&nbsp;Robert Săndulescu ,&nbsp;Cecilia Cristea","doi":"10.1016/j.protcy.2017.04.046","DOIUrl":"10.1016/j.protcy.2017.04.046","url":null,"abstract":"<div><p>Two different graphene/<em>β</em>-cyclodextrin (CD)-based biosensors were elaborated for dopamine (DA) determination starting from glassy carbon electrode (GCE). First one was obtained by modifying GCE with reduced graphene oxide (RGO), CD and tyrosinase (Tyr) immobilized with a polyethylenimine film. The second biosensor was obtained after the incorporation of RGO functionalized with a new synthesized pyrrole-<em>β</em>-CD derivative and Tyr in an electropolimerized poly-amphiphilic pyrrole film. These two new approaches have resulted in selective and sensitive determination of DA in pharmaceuticals and real human samples.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 106-107"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81673180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics on Integrated Circuit","authors":"Boon Chong Cheah ,&nbsp;Alasdair I. MacDonald ,&nbsp;Michael P. Barrett ,&nbsp;David R.S. Cumming","doi":"10.1016/j.protcy.2017.04.025","DOIUrl":"10.1016/j.protcy.2017.04.025","url":null,"abstract":"<div><p>We have demonstrated a chip-based diagnostics tool for the quantification of metabolites, using specific enzymes, to study enzyme kinetics and calculate the Michaelis-Menten constant. An array of 256×256 ion-sensitive field effect transistors (ISFETs) fabricated in a complementary metal oxide semiconductor (CMOS) process is used for this prototype. We have used hexokinase enzyme reaction on the ISFET CMOS chip with glucose concentration in the physiological range of 0.05 mM – 231 mM and successfully studied the enzyme kinetics of hexokinase in detail. This will promote future research towards multiplexing enzyme-based metabolite quantification on a single chip, ultimately opening a pathway towards a personal metabolome machine.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 53-54"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73522402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screen Printed Electromechanical Micro-total Analysis System (μtas) for Sensitive and Rapid Detection of Infectious Diseases","authors":"Anis Nurashikin Nordin ,&nbsp;Ahmad Anwar Zainuddin ,&nbsp;Rosminazuin Ab Rahim ,&nbsp;Ioana Voiculescu ,&nbsp;Wing Cheung Mak","doi":"10.1016/j.protcy.2017.04.043","DOIUrl":"10.1016/j.protcy.2017.04.043","url":null,"abstract":"&lt;div&gt;&lt;p&gt;The main objective of this article is to demonstrate by performing simulation measurements of biosensor that can detect the presence of pathogens through simultaneous mass and impedance techniques. This biosensor merges two biosensing techniques namely resonant frequency measurements and electrochemical impedance spectroscopy (EIS) on a single biosensor. Parallel measurements provide better sensitivities, have higher diagnostics accuracy and reduce the risk of false positives. Low cost, high resolution screen printing technology was used to fabricate the microelectromechanical array of μTAS on flexible piezoelectric substrates. The basic biosensor framework includes a substrate that highly sensitive sensor like thickness shear mode and immunosensor can be fabricated using quartz crystal lattice that integrated with electrochemical sensor [1]. The quartz crystal microbalance is a label free technique, which minimizes interference with the interaction being studied. A piezoelectric device is portable, simple and cost effective, and is suitable for real-time monitoring of biospecific interactions such as antigen-antibody, receptor ligand, and enzymes-substrate interactions with high sensitivity and specificity. For instance, the biological mixtures such as antibodies are capable of binding to terminal active functional groups (&lt;em&gt;i.e.&lt;/em&gt;, COOH, OH and NH&lt;sub&gt;2&lt;/sub&gt;) of self-assembled monolayers (SAM) and immunocapture antigens such as glycoproptien or other targets[2]. The QCM can consequently detect mass changes due to these molecular interactions on the surface of the QCM. The top and bottom circular excitation electrodes with 150um diameter were modeled as gold (Au) films of 16 μm thickness. A sinusoidal voltage with amplitude of 5 mV was applied across the quartz crystal. Figure 1 shows the principle of integrated biosensors which gold electrodes were printed on both sides of a thin 500um quartz layer to form the quartz crystal microbalance (QCM)-impedance device. The silver (Ag) semicircular counter electrode was modeled around the top working electrode on the same area of the quartz crystal for performing the electrochemical impedance spectroscopy (EIS) experiments for detection of bacteria (E-Coli) and the results were compared to quartz crystal microbalance measurements. Furthermore, the use of gold surface can be incorporated into the transducer compatible with the biological samples such as use of highly specific monoclonal antibodies, and incorporation of amplification step to maximize the signal detection. In general, the quartz crystal is traditionally considered to be a mass sensitive sensor that produces response which it changes its resonance frequency to different thin film samples or liquids in contact with it surface. For a straight relationship between a thin film mass of the order of nanograms, the quartz crystal response will be of of the order of Hertz according to Eq. 1, Sauerbrey Equation [3]. &lt;em&gt;ρ&lt;/em&gt;&lt;sub&gt;&lt;em&gt;","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 100-101"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79744643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adding Biomolecular Recognition Capability to 3D Printed Objects: 4D Printing","authors":"C.A. Mandon,&nbsp;L.J. Blum,&nbsp;C.A. Marquette","doi":"10.1016/j.protcy.2017.04.001","DOIUrl":"10.1016/j.protcy.2017.04.001","url":null,"abstract":"<div><p>3D printing technologies will impact in a near future the biosensor community, both at the sensor prototyping level and the sensing layer organization level. The present study aimed at demonstrating the capacity of one 3D printing technique, the Digital Light Processing (DLP), to produce hydrogel sensing layers with 3D shapes unreachable using conventional molding procedures but still biosensing activity (4D printed objects).</p><p>The first model of sensing layer was composed of a sequential enzymatic reaction (glucose oxidase and peroxidase) and the generated chemiluminescent reaction in the presence of glucose and luminol used as analytical signal. Highly complex objects (<em>fancifuball, puzzle pieces, 3D pixel, propellers, fluidic, multi-compartments</em>) with mono-, di- and tri-components configurations were achieved and the activity of the encapsulated enzymes demonstrated.</p><p>The second model was a sandwich immunoassay protocol for the detection of Brain Natriuretic Peptide. Here, highly complex propeller shape sensing layers were produced and the recognition capability of the antibodies demonstrated.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91054039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}