Procedia Technology最新文献

{"title":"Culture Mediums and Buffer Effect on Screen-printed Carbon Electrodes for Continuous Voltammetric Monitoring of in vitro Cell Cultures Lactate Production","authors":"G. Rosati, M. Scaramuzza, V. Rotilio, L. Monaco, E. Pasqualotto, F. Campolo, A. Toni, C. Reggiani, F. Naro, A. Paccagnella","doi":"10.1016/J.PROTCY.2017.04.105","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.105","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"22 1","pages":"246-247"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90489991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Automatic Miniature Surface Plasmon Resonance System for Cortisol Detection","authors":"Lulu Zhang ,&nbsp;Xing Chen ,&nbsp;Wei Wei ,&nbsp;Shaoli Deng ,&nbsp;Chunfang Xu ,&nbsp;Dafu Cui","doi":"10.1016/j.protcy.2017.04.038","DOIUrl":"10.1016/j.protcy.2017.04.038","url":null,"abstract":"<div><p>This paper presents a homemade miniature surface plasmon resonance (SPR) system with automatic sample injection device which is able to work 24 hours unattended. The system with a volume of 48cm×25cm×28 cm was constructed with a red laser light source, a P-polarizer, a triangle glass prism, a linear CCD detector, gold sensor chips modified with BSA or colloidal gold, an automatic sample injection device with 20 sample locations. The system sensitivity and noise were tested through detecting different concentration of glycerol solutions with a group of refractive indices (RIs). Cortisol-BSA was fixed on the gold sensor chips modified with BSA or colloidal gold. A series concentration of cortisol (1, 10, 100, 1000 ng/ml) mixed with cortisol-specific monoclonal antibodies were detected by miniature SPR system. The performance of BSA and colloidal gold modified sensor chips were compared by calculating the standard curves and the kinetic curves of the cortisol detection. The results showed that the BSA sensor chip could get a better detection limit of cortisol sample while the colloidal gold sensor chip owed a higher stability and repeatability by regenerating the antibody surface. The research studied the performance of the homemade SPR system and the sensor chips which could be widely used in the protein measurement areas.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 89-90"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77993426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Biosensing Strategy for Fast Profiling of Antibiotic Resistance","authors":"Michael Mecklenburg ,&nbsp;Qun Chen ,&nbsp;Anneli Andersson ,&nbsp;Bin Xie","doi":"10.1016/j.protcy.2017.04.016","DOIUrl":"10.1016/j.protcy.2017.04.016","url":null,"abstract":"<div><p>Antibiotic resistance threatens global public health. Clinical methods that simplify and accelerate resistance diagnosis are urgently needed. Here we describe a function-based antibiotic resistance detection and classification strategy to improve diagnosis. The method identifies resistance enzymes by directly measuring the thermal signal generated when an antibiotic i enzymatically degraded. A substrate specificity profile is created by analyzing a panel of antibiotics. Here we show proof of principle by differentiating two antibiotic resistance enzymes based on their substrate specificities profiles. The method provides a fast, simple, cost effective alternative for diagnosing and classifying antibiotic resistance.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 33-34"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77758853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simplified Immuno-assay for Rapid Label-free Dengue Serotype Diagnosis: Suppression of Non-specific Binding Interference","authors":"F.C.L. Loureiro ,&nbsp;A.M.N. Lima ,&nbsp;R. Roque ,&nbsp;R.M.P. de Figueiredo ,&nbsp;E.U.K. Melcher ,&nbsp;M. Borre ,&nbsp;C. Thirstrup ,&nbsp;H. Neff","doi":"10.1016/j.protcy.2017.04.031","DOIUrl":"10.1016/j.protcy.2017.04.031","url":null,"abstract":"<div><p>An easy to implement immunoassay for rapid dengue virus (DENV) serotype diagnosis in the early infection stage is reported. The four-layer assay is immobilized onto a thin gold film and relies on a polymer biochip for optical surface plasmon resonance sensing and detection. The protocol comprises Neutravidin-Biotin mediated DENV monoclonal antibody (MAB_D) attachment as the functionalized sensing element. The MAB_D-DENV complex formation results in a pronounced thickness change that is optically detected in real time. The protocol is applicable in presence of strong non-specific binding interference. False positive indications for negative serum and blood control samples were not observed. The method can be modified towards detection of other viruses infections.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 67-69"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84624231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-low-Light CMOS Biosensor Complements Microfluidics to Achieve Portable Diagnostics","authors":"Zhimin Ding,&nbsp;Cai Xu,&nbsp;Yifang Wang,&nbsp;Gianfranco Pellegrini","doi":"10.1016/j.protcy.2017.04.019","DOIUrl":"10.1016/j.protcy.2017.04.019","url":null,"abstract":"<div><p>Innovations in ultra low-light CMOS bio-optical sensor design, combined with microfluidics technology can enable a new generation of low cost, portable molecular diagnostics platforms. This combination of technologies have been applied successfully in a miniaturized qPCR system, and a chemiluminescence microfluidic immunoassay platform to detect a variety of infectious pathogens. Cost reduction and miniaturization of molecular test enabled by this technology will have positive impact on global battle against infectious diseases.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 39-41"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78148135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrocatalytic Evaluation of a Horseradish Peroxidase Biosensor Based on a Novel Bi-Ag Bimetallic Nanocomposite","authors":"Charlton van der Horst ,&nbsp;Bongiwe Silwana ,&nbsp;Emmanuel Iwuoha ,&nbsp;Vernon Somerset","doi":"10.1016/j.protcy.2017.04.077","DOIUrl":"10.1016/j.protcy.2017.04.077","url":null,"abstract":"<div><p>The present work illustrates the construction and application of bismuth-silver bimetallic nanoparticles (Bi-AgNPs) in biosensor construction for the detection of peroxide (H₂O₂). The biosensor was constructed by drop-casting of horseradish peroxidase onto the Bi-AgNPs/GCE sensor. Impedance analysis revealed that the <em>R</em>_ct value for the bare GCE obtained was 2.548 × 10³ Ω and increased to 5.768 × 10³ Ω for the HRP/Bi-AgNPs/GCE biosensor. This increased value of <em>R</em>_ct indicated hindrance to the electron transfer, providing evidence for the immobilization of insulating HRP on the Bi-AgNPs/GCE surface. The HRP/Bi-AgNPs/GCE biosensor was successfully applied for sensitive detection of H₂O₂ by differential pulse voltammetry. Well defined peaks were observed with a low linearity range from 0.02 × 10⁻⁶ to 1.0 × 10⁻⁶ M (<em>R</em>² = 0.991), with a limit of detection of 0.06<!--> <!-->μM. The results obtained for the HRP/Bi-AgNPs/GCE biosensor compared well within the range of similar biosensors reported.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 179-182"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89854510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic-particle Based Signal Amplification Method Integrated with Mobile-devices for Low Cost Biosensing","authors":"Omary Mzava,&nbsp;Zehra Taş,&nbsp;Vahit Can Lafcı,&nbsp;Mehmet Akif Çakar,&nbsp;İbrahim Özdür,&nbsp;Kutay İçöz","doi":"10.1016/j.protcy.2017.04.008","DOIUrl":"10.1016/j.protcy.2017.04.008","url":null,"abstract":"<div><p>We present a signal amplification method for biosensing applications using magnetic particles. In this method, mobile devices and simple spherical glass beads are used as a low-cost microscope to detect magnetic particles. Magnetic particles have two main functions; 1) conventionally capture, separate and transport target molecules 2) form magnetic dipoles under an applied external magnetic field to attract other magnetized particles. When magnetic particles accumulate and form a cluster, the corresponding pixel area in the image taken by the simple microscope is increased resulting in signal amplification.</p><p>Current focus of new generation biosensor research is to increase the sensitivity levels of the devices to compete with current lab analysis tools while inherently having other advantages such as being low-cost, portable and simple. Biosensors based on micro/nano magnetic particles use various measurement techniques and amplification methods. In order to fully benefit from the advantages of micro/nano technology based systems, measurement set up must be also portable and have high sensitivity. Mobile devices and applications are taking place in medical fields and have high potential for future. In this work mobile devices are employed as measurement setups for the magnetic particle based sensing and signal amplification. The amplification method is not based on bimolecular binding thus cost efficient. After the images of the magnetic particles are taken, these images are sent to cloud computing for analysis by the mobile device. Matlab codes run on cloud servers for processing the images. Finally results are received and displayed on the mobile device.</p><p>The mobile device based imaging system is able to detect 7 μm size particles within a 1500 μm x1500 μm area and magnetic bead accumulation resulted in at least 5-fold signal amplification. The applied magnetic field is approximately 15 mT and the cost of the system excluding mobile device is under 20 cents. The method is promising for immunomagnetic bead assisted biosensors.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 14-15"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82102995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured Photoelectrochemical Biosensing Platform for Cancer Biomarker Detection","authors":"Diego Voccia ,&nbsp;Francesca Bettazzi ,&nbsp;Serena Laschi ,&nbsp;Cristina Gellini ,&nbsp;Giangaetano Pietraperzia ,&nbsp;Luigi Falciola ,&nbsp;Valentina Pifferi ,&nbsp;Chiara Ingrosso ,&nbsp;Tiziana Placido ,&nbsp;Roberto Comparelli ,&nbsp;M. Lucia Curri ,&nbsp;Ilaria Palchetti","doi":"10.1016/j.protcy.2017.04.063","DOIUrl":"10.1016/j.protcy.2017.04.063","url":null,"abstract":"<div><p>The innovative photoelectrochemical properties of multifunctional nanomaterials are here investigated for the development of biosensing platforms for rapid and sensitive detection of a class of cancer biomarker candidates, known as microRNAs. Many different transducers have been proposed, so far, for microRNA detection. Recently, with the emergence of novel photoelectrochemically active species and new detection schemes, photoelectrochemistry has received increasing attention. Gold nanostructures have been, here, used to modify TiO₂ electrodes. The surface of the nanostructured platform has been modified by nucleic acid capture probes (CPs). Biotinylated target miRNAs have been recognized by the specific CPs. The biosensing platform has been incubated with streptavidin alkaline phosphatase and exposed to a proper substrate. The product of the enzymatic reaction has been photoelectrochemically monitored. A compact and hand-held analytical device has been developed in order to have a final prototype in line with the concept of point of care testing.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 144-145"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81280304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring in Vivo Behaviors of Protein Nanocages via Encapsulating an NIR-II Ag2S Quantum Dot","authors":"Chunyan Li ,&nbsp;Feng Li ,&nbsp;Yejun Zhang ,&nbsp;Wenjing Zhang ,&nbsp;Xian-En Zhang ,&nbsp;Qiangbin Wang","doi":"10.1016/j.protcy.2017.04.027","DOIUrl":"10.1016/j.protcy.2017.04.027","url":null,"abstract":"<div><h3>Introduction</h3><p>Protein nanocages (PNCs) have been recognized as a promising platform for nanomedicine innovation. Real-time <em>in vivo</em> tracking of PNCs can provide critically important information for the development of PNC-based diagnostics and therapeutics. Here we demonstrate a strategy for monitoring the behaviors of PNCs <em>in vivo</em> by encapsulating a Ag₂S quantum dot (QD) with fluorescence in the second near-infrared window (NIR-II, 1000-1700 nm) inside the PNC, using a simian virus 40 (SV40) PNC (PNC_SV40) as a model.</p></div><div><h3>Methods</h3><p>The Ag₂S QD was encapsulated into the PNC_SV40 through controllable molecular self-assembly. The dynamic migration of Ag₂S@PNC_SV40 in living mouse was tracked in real time under an InGaAs-based shortwave infrared imaging system and was further corroborated by <em>ex vivo</em> imaging, inductively coupled plasma mass spectrometry analysis, and macrophage endocytosis assay.</p></div><div><h3>Results</h3><p>Benefitting from the high spatiotemporal resolution and deep tissue penetration of NIR-II fluorescence imaging, the dynamic distribution of the PNC_SV40 in living mice was tracked in real time with high fidelity, revealing rapid clearance from bloodstream within 5<!--> <!-->min post-intravenous injection and selective accumulation in liver, spleen and bone marrow. Furthermore, adopting the PEGylation strategy, PEGylated PNC_SV40 presents remarkably different behaviors <em>in vivo</em> with significantly prolonged blood circulation and much less uptake in the reticuloendothelial system (RES), leading to desirable pharmacokinetics and pharmacodynamics of PNC-based nanomedicines.</p></div><div><h3>Discussion</h3><p>This study represents the first evidence of real-time tracking of the intrinsic behaviors of PNCs <em>in vivo</em> without interference in PNC-host interactions by encapsulating nanoprobes inside, instead of conjugating nanoprobes onto the outer surface of PNCs. The as-described imaging strategy will facilitate the study of interactions between exogenously introduced PNCs and host body, prompting the development of future protein-based drugs, high-efficacy targeted delivery system, sensors, etc.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 57-58"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82216043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paper-based Stencil-free Enzymatic Sensor with Ink and Wire Electrodes","authors":"O. Amor-Gutiérrez,&nbsp;E. Costa Rama,&nbsp;A. Costa-García,&nbsp;M.T. Fernández-Abedul","doi":"10.1016/j.protcy.2017.04.055","DOIUrl":"10.1016/j.protcy.2017.04.055","url":null,"abstract":"<div><p>Analytical Chemistry is nowadays one of the branches of Chemistry that has changed enormously due to the advances in technology and the trends it follows. Currently, the development of low-cost devices that are, at the same time, easy-to use and dispose, and produce fast and reliable responses is of eminent significance. Electrochemical biosensors fit perfectly with these requirements, as do paper-based devices. Here we present an enzymatic biosensor using a simple single-use paper-based device in which carbon ink is deposited in a hydrophilic area delimited by wax printing for acting as working electrode. Low-cost gold-plated connector headers are employed as reference and auxiliary electrodes as well as connections to the potentiostat. Glucose oxidase (GOx), horseradish peroxidase (HRP) and potassium ferrocyanide used as mediator of the electron transfer are adsorbed in the ink.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 126-128"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79778438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}