Procedia Technology最新文献

{"title":"Mucin 4 Immunosensor Based on p-aminophenylacetic Acid Grafting on Carbon Electrodes as Immobilization Platform","authors":"Oana Hosu,&nbsp;Mihaela Tertiş,&nbsp;Gheorghe Melinte,&nbsp;Robert Săndulescu,&nbsp;Cecilia Cristea","doi":"10.1016/j.protcy.2017.04.048","DOIUrl":"10.1016/j.protcy.2017.04.048","url":null,"abstract":"<div><p>A simple impedimetric label-free immunosensor was developed for the specific and sensitive detection of mucin 4 (MUC 4) protein by using graphite based screen printed electrodes modified with an aryl diazonium salt or compound (<em>p</em>- aminophenylacetic acid) for the immobilization of antibody anti-MUC4 via amidic bond. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used in order to characterize and optimize the electrografting process. The parameters involved in each step of the immunosensor design were optimized. The performance of the immunoassay in terms of sensitivity, reproducibility and selectivity was studied.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89168197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Electrochemical Scaffolds for Multiplexed Biosensing of Cancer Reporters in Complex Clinical Samples","authors":"Rebeca M. Torrente-Rodríguez ,&nbsp;Susana Campuzano ,&nbsp;Víctor Ruiz-Valdepeñas Montiel ,&nbsp;Unai Eletxigerra ,&nbsp;Josu Martinez-Perdiguero ,&nbsp;Santos Merino ,&nbsp;Rodrigo Barderas ,&nbsp;Reynaldo Villalonga ,&nbsp;José M. Pingarrón","doi":"10.1016/j.protcy.2017.04.010","DOIUrl":"10.1016/j.protcy.2017.04.010","url":null,"abstract":"<div><p>Early and reliable diagnostic of cancer is mandatory to increase patient survival, thus requiring efficient and reliable analytical methods for such a purpose. Within this context, different strategies implying the development of electrochemical biosensors for the sensitive, selective and rapid multiplexed biosensing of genetic or protein cancer-related biomarkers are addressed in this presentation. In particular, novel sensing platforms have been developed for the determination of miRs, interleukin (IL)-8 mRNA, IL-8 protein, and cancer specific receptors. The developed methodologies allow for the determination of the target analytes at clinically relevant levels in complex samples: cancer cells, human tissues cell lysates, serum and raw saliva and can be easily extended to the determination of other relevant biomarkers.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87924915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Prototyping of a Low-cost Graphene-based Impedimetric Biosensor","authors":"S. Popescu, C. Dale, N. Keegan, B. Ghosh, R. Kaner, J. Hedley","doi":"10.1016/J.PROTCY.2017.04.116","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.116","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90765416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel QCM-based Method to Predict in Vivo Behaviour of Nanoparticles","authors":"M. Gianneli ,&nbsp;Y. Yan ,&nbsp;E. Polo ,&nbsp;D. Peiris ,&nbsp;T. Aastrup ,&nbsp;K.A. Dawson","doi":"10.1016/j.protcy.2017.04.084","DOIUrl":"10.1016/j.protcy.2017.04.084","url":null,"abstract":"<div><p>In biological fluids, proteins and other biomolecules bind to the surface of nanoparticles to form a coating known as the protein corona which in turn becomes primary determinant of the nanoparticles’ fate and behaviour. Here we develop a QCM-based platform and methodology to obtain data from real-time interactions of nanoparticles with selected human plasma proteins. Polystyrene particles coated with transferrin are immobilized on QCM sensor chips and by means of a ‘sandwich’ format binding assay, specific epitopes on the particles can be quantified as measured by the increase of the sensor's resonant frequency. Cell binding experiments where adherent cells are directly grown on the sensor surface are also performed. Interaction of nanoparticles injected over the cell surface is observed only in the case of particle-transferrin complexes demonstrating that it is the nanoparticle-corona complex, rather than the native nanoparticle, “what the cell sees”, with the corona being the interface between the nanoparticle and the cellular system. Our data highlight the potential of the proposed QCM-based platform and methodology for characterization of the bio-nano-interface and tracking the interaction of nanoparticles with biological cells in the presence of a realistic milieu.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87615176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalization of Gold-plasmonic Devices for Protein Capture","authors":"E. Battista ,&nbsp;P.L. Scognamiglio ,&nbsp;G. Das ,&nbsp;G. Manzo ,&nbsp;F. Causa ,&nbsp;E. Di Fabrizio ,&nbsp;P.A. Netti","doi":"10.1016/j.protcy.2017.04.071","DOIUrl":"10.1016/j.protcy.2017.04.071","url":null,"abstract":"<div><p>Here we propose a straightforward method to functionalize gold nanostructures by using an appropriate peptide sequence already selected toward gold surfaces and derivatized with another sequence for the capture of a molecular target. Large scale 3D-plasmonic devices with different nanostructures were fabricated by means of direct nanoimprint technique. The present work is aimed to address different innovative aspects related to the fabrication of large-area 3D plasmonic arrays, their direct and easy functionalization with capture elements, and their spectroscopic verifications through enhanced Raman and enhanced fluorescence techniques.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90176084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Molecular Diagnosis of Bacterial Infection Using Integrated Lab-on-a-disc","authors":"J. Loo ,&nbsp;C.C.H. Leung ,&nbsp;H.C. Kwok ,&nbsp;S.Y. Wu ,&nbsp;I.L.G. Law ,&nbsp;M.L. Chin ,&nbsp;M. Hui ,&nbsp;S.K. Kong ,&nbsp;H.P. Ho","doi":"10.1016/j.protcy.2017.04.094","DOIUrl":"10.1016/j.protcy.2017.04.094","url":null,"abstract":"<div><p>Bacterial infection is a disaster leading to high fatal rate in intensive caring unit. Rapid profiling of infectious bacteria is necessary for applying the correct medication. Current gold standard using plate inoculation is inaccurate, time-consuming and labour-intensive. Therefore we have developed a molecular diagnostic approach to target marker DNA of the infectious bacteria for rapid profiling. A micro-fluidic platform lab-on-a-disc (LOAD) has been adopted because using one simple spinning action can provide highly controllable centrifugation drive force for the actuation of samples and reagents anywhere within the boundary of the disc. When centrifugal force-triggered valve is applied, complex sequential flow of liquid can be controlled with various centrifugal force. This will enable parallel execution of many reactions simultaneously with minimal complexity in the design of fluidic pumping and flow control.</p><p>We report an integrated LOAD for direct sample-to-answer applications - fully automated assay from patient's sample input to detection of signal output. The integrated LOAD with PDMS-made microfludic disc performs three major functions, namely DNA extraction, LAMP reaction and detection. Using microfluidics technology, target bacteria can be detected using as little as 10 μL blood sample loaded into sample loading site. The DNA release after cell lysis in heating site is bound on the silica membrane. After washing, the purified DNA elution is subjected to LAMP reaction to amplify the target genetic sequence. Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification method where reaction occurs under 65 ^oC. The amplified signal is reported by DNA binding fluorescent dye. Our prototype shows high yield and purity of bacterial DNA from clinical samples such as blood. We demonstrated the detection of <em>Acinetobacter baumanii</em>, which is one of the key pathogens resulting in hospital-acquired infections, in clinical blood sample using the LOAD platform. Fast signal detection and active temperature control within the LOAD platform has also enabled real-time LAMP targeting of specific DNA sequences as barcodes to identify infected bacterial species. We found the detection sensitivity of LAMP using DNA is 10^-15 g, while that of bacteria concentration is 10² cfu/ml. The system is capable of providing bacterial DNA profiling within 2 hours.</p><p>In conclusion, our integrated LOAD is a simple (sample-to-answer), specific (specific genetic sequences recognition), robust (automated assay on microfluidic disc) method for rapid molecular diagnosis of bacterial infection. The short turnaround time and the technical advancement of sample-to-answer in one LOAD platform approach for rapid bacterial detection should have much potential in addressing the needs of point-of-care medical diagnosis applications. The simplicity allows the clinical healthcare workers to utilize ","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90203039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of Gold Electrodes with Bacterial Reaction Centres Immobilized by Laser Induced Forward Transfer (LIFT) Technique for Amperometric Herbicide Detection","authors":"M.R. Guascito ,&nbsp;M. Chatzipetrou ,&nbsp;D. Chirizzi ,&nbsp;M. Trotta ,&nbsp;M. Massaouti ,&nbsp;L. Giotta ,&nbsp;F. Milano ,&nbsp;I. Zergioti","doi":"10.1016/j.protcy.2017.04.083","DOIUrl":"10.1016/j.protcy.2017.04.083","url":null,"abstract":"<div><p>The functionalization of screen-printed electrodes (SPEs) with a thin film of reaction centre (RC) proteins from the phototrophic bacterium <em>Rhodobacter (R.) sphaeroides</em>, by means of laser induced forward transfer (LIFT) technique, allowed the fabrication of robust and sensitive bio-hybrid devices for terbutryn detection and analysis. The optimal wiring between RCs and the gold electrode surface, achieved by LIFT, led to the generation of cathodic photocurrents sustained by a direct electron transfer (DET) mechanism, which were attenuated by addition of the herbicide inhibitor.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89132798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Protein Cleavage in a Microfluidic Cell for Proteomics Studies","authors":"Floris T.G. van den Brink ,&nbsp;Tao Zhang ,&nbsp;Liwei Ma ,&nbsp;Mathieu Odijk ,&nbsp;Wouter Olthuis ,&nbsp;Hjalmar P. Permentier ,&nbsp;Rainer P.H. Bischoff ,&nbsp;Albert van den Berg","doi":"10.1016/j.protcy.2017.04.029","DOIUrl":"10.1016/j.protcy.2017.04.029","url":null,"abstract":"<div><p>Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77296025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Automatic Miniature Surface Plasmon Resonance System for Cortisol Detection","authors":"Lulu Zhang ,&nbsp;Xing Chen ,&nbsp;Wei Wei ,&nbsp;Shaoli Deng ,&nbsp;Chunfang Xu ,&nbsp;Dafu Cui","doi":"10.1016/j.protcy.2017.04.038","DOIUrl":"10.1016/j.protcy.2017.04.038","url":null,"abstract":"<div><p>This paper presents a homemade miniature surface plasmon resonance (SPR) system with automatic sample injection device which is able to work 24 hours unattended. The system with a volume of 48cm×25cm×28 cm was constructed with a red laser light source, a P-polarizer, a triangle glass prism, a linear CCD detector, gold sensor chips modified with BSA or colloidal gold, an automatic sample injection device with 20 sample locations. The system sensitivity and noise were tested through detecting different concentration of glycerol solutions with a group of refractive indices (RIs). Cortisol-BSA was fixed on the gold sensor chips modified with BSA or colloidal gold. A series concentration of cortisol (1, 10, 100, 1000 ng/ml) mixed with cortisol-specific monoclonal antibodies were detected by miniature SPR system. The performance of BSA and colloidal gold modified sensor chips were compared by calculating the standard curves and the kinetic curves of the cortisol detection. The results showed that the BSA sensor chip could get a better detection limit of cortisol sample while the colloidal gold sensor chip owed a higher stability and repeatability by regenerating the antibody surface. The research studied the performance of the homemade SPR system and the sensor chips which could be widely used in the protein measurement areas.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77993426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic-particle Based Signal Amplification Method Integrated with Mobile-devices for Low Cost Biosensing","authors":"Omary Mzava,&nbsp;Zehra Taş,&nbsp;Vahit Can Lafcı,&nbsp;Mehmet Akif Çakar,&nbsp;İbrahim Özdür,&nbsp;Kutay İçöz","doi":"10.1016/j.protcy.2017.04.008","DOIUrl":"10.1016/j.protcy.2017.04.008","url":null,"abstract":"<div><p>We present a signal amplification method for biosensing applications using magnetic particles. In this method, mobile devices and simple spherical glass beads are used as a low-cost microscope to detect magnetic particles. Magnetic particles have two main functions; 1) conventionally capture, separate and transport target molecules 2) form magnetic dipoles under an applied external magnetic field to attract other magnetized particles. When magnetic particles accumulate and form a cluster, the corresponding pixel area in the image taken by the simple microscope is increased resulting in signal amplification.</p><p>Current focus of new generation biosensor research is to increase the sensitivity levels of the devices to compete with current lab analysis tools while inherently having other advantages such as being low-cost, portable and simple. Biosensors based on micro/nano magnetic particles use various measurement techniques and amplification methods. In order to fully benefit from the advantages of micro/nano technology based systems, measurement set up must be also portable and have high sensitivity. Mobile devices and applications are taking place in medical fields and have high potential for future. In this work mobile devices are employed as measurement setups for the magnetic particle based sensing and signal amplification. The amplification method is not based on bimolecular binding thus cost efficient. After the images of the magnetic particles are taken, these images are sent to cloud computing for analysis by the mobile device. Matlab codes run on cloud servers for processing the images. Finally results are received and displayed on the mobile device.</p><p>The mobile device based imaging system is able to detect 7 μm size particles within a 1500 μm x1500 μm area and magnetic bead accumulation resulted in at least 5-fold signal amplification. The applied magnetic field is approximately 15 mT and the cost of the system excluding mobile device is under 20 cents. The method is promising for immunomagnetic bead assisted biosensors.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82102995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}