Advanced Electrochemical Scaffolds for Multiplexed Biosensing of Cancer Reporters in Complex Clinical Samples

Procedia Technology Pub Date : 2017-01-01 DOI:10.1016/j.protcy.2017.04.010

Rebeca M. Torrente-Rodríguez , Susana Campuzano , Víctor Ruiz-Valdepeñas Montiel , Unai Eletxigerra , Josu Martinez-Perdiguero , Santos Merino , Rodrigo Barderas , Reynaldo Villalonga , José M. Pingarrón

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Abstract

Early and reliable diagnostic of cancer is mandatory to increase patient survival, thus requiring efficient and reliable analytical methods for such a purpose. Within this context, different strategies implying the development of electrochemical biosensors for the sensitive, selective and rapid multiplexed biosensing of genetic or protein cancer-related biomarkers are addressed in this presentation. In particular, novel sensing platforms have been developed for the determination of miRs, interleukin (IL)-8 mRNA, IL-8 protein, and cancer specific receptors. The developed methodologies allow for the determination of the target analytes at clinically relevant levels in complex samples: cancer cells, human tissues cell lysates, serum and raw saliva and can be easily extended to the determination of other relevant biomarkers.

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用于复杂临床样品中癌症报告者多重生物传感的先进电化学支架

早期可靠的癌症诊断是提高患者生存率的必要条件，因此需要高效可靠的分析方法来实现这一目的。在此背景下，本文讨论了电化学生物传感器的不同发展策略，以实现对遗传或蛋白质癌症相关生物标志物的敏感、选择性和快速多路生物传感。特别是，新的传感平台已经开发用于测定mir，白细胞介素(IL)-8 mRNA, IL-8蛋白和癌症特异性受体。开发的方法允许在复杂样品中测定临床相关水平的目标分析物:癌细胞，人体组织细胞裂解物，血清和原始唾液，并且可以很容易地扩展到其他相关生物标志物的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Procedia Technology

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