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Lossy Mode Resonance-based Aptasensor for CRP Detection 基于有损模式共振的CRP检测传感器☆
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.069
P. Sanchez , P. Zubiate , F.J. Munoz , F.J. Arregui , I.R. Matias , C.R. Zamarreño
{"title":"Lossy Mode Resonance-based Aptasensor for CRP Detection","authors":"P. Sanchez ,&nbsp;P. Zubiate ,&nbsp;F.J. Munoz ,&nbsp;F.J. Arregui ,&nbsp;I.R. Matias ,&nbsp;C.R. Zamarreño","doi":"10.1016/j.protcy.2017.04.069","DOIUrl":"10.1016/j.protcy.2017.04.069","url":null,"abstract":"<div><p>In this work, a CRP detection by Aptasensor based on LMR is presented. A thin film of Tin Dioxide (SnO<sub>2</sub>) has been used as LMR generator, and the CRP Aptamer has blind onto the optical fiber to perform the measures.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75465040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
NFC Based Smart Biosensor – An Introduction to Battery-less Enzymatic Amperometric Glucose Sensor Based on NFC Technology 基于NFC的智能生物传感器——介绍基于NFC技术的无电池酶促安培葡萄糖传感器
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.021
C. Matoschitz, R. Lurf, M. Meindl, M. Beisteiner, M. Bammer
{"title":"NFC Based Smart Biosensor – An Introduction to Battery-less Enzymatic Amperometric Glucose Sensor Based on NFC Technology","authors":"C. Matoschitz,&nbsp;R. Lurf,&nbsp;M. Meindl,&nbsp;M. Beisteiner,&nbsp;M. Bammer","doi":"10.1016/j.protcy.2017.04.021","DOIUrl":"10.1016/j.protcy.2017.04.021","url":null,"abstract":"<div><p>Biosensors are widely used as analytical devices for determination of medically relevant parameters, e.g. glucose, lactate and histamine. The possibility of easily available and low-cost sensors is a central need in mobile diagnostics and personal health monitoring. Therefore, biosensors combined with near field communication (NFC) technology enable simple and smart sensor solutions for electrical and non-electrical parameter measurements.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74251042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
LSPR Biosensor Based on Nanostructured Gold Films: Detection of Mycotoxins 基于纳米结构金膜的LSPR生物传感器:真菌毒素检测
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.057
Ali Ghamin Al-Rubaye , Alexei Nabok , Anna Tsargorodska
{"title":"LSPR Biosensor Based on Nanostructured Gold Films: Detection of Mycotoxins","authors":"Ali Ghamin Al-Rubaye ,&nbsp;Alexei Nabok ,&nbsp;Anna Tsargorodska","doi":"10.1016/j.protcy.2017.04.057","DOIUrl":"10.1016/j.protcy.2017.04.057","url":null,"abstract":"<div><p>This work focuses on the development of optical sensors based on the LSPR phenomenon in nano-structured gold films suitable for detection of mycotoxins. A simple technology of annealing thin gold films was utilized for the formation of gold nano-islands exhibiting the LSPR effect. The morphology of gold nano-structures produced was studied with SEM and AFM while their optical properties were analysed with UV-vis absorption spectroscopy and spectroscopic ellipsometry (SE). A blue spectral shift of LSPR band caused by changes in the refractive index of medium constitutes the main principle of LSPR sensing. Bio-sensing tests were attempted using SE in total internal reflection mode (TIRE); a noticeable spectral shift was recorded on course of immune binding of aflatoxin B1 to specific antibodies immobilized on the surface of gold.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74587386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Creating Aptamers and Their Use in Resisitive Pulse Sensors 适配体的创建及其在电阻式脉冲传感器中的应用
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.007
Mark Platt
{"title":"Creating Aptamers and Their Use in Resisitive Pulse Sensors","authors":"Mark Platt","doi":"10.1016/j.protcy.2017.04.007","DOIUrl":"10.1016/j.protcy.2017.04.007","url":null,"abstract":"<div><p>Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse small channels. Here we present our recent advancement of the technique identifying key experimental designs for potential POC assays. The first assay utilized superparamagnetic beads, if the surfaces of the beads are modified with an aptamer, the frequency of beads (translocations/minute) through the pore can be related to the concentration of specific proteins in the solution. Herein, we have demonstrated the successful use of TRPS to observe the binding of two proteins, to their specific aptamers simultaneously. We then adapt the measurement strategy and demonstrate that the translocation times of particles can be used to infer the zeta potential to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridisation time, we observe a clear difference in zeta potential using both mean values, and population distributions as a function of the DNA structure. Finally we present the first comparison between assays that use resistive pulses or rectification ratios on a tunable pore platform.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74784331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering 基于介质电泳和过滤的高通量微流控稀有细胞富集系统
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.076
Gürhan Özkayar , Yağmur Demircan Yalçın , Ebru Özgür , Ufuk Gündüz , Haluk Külah
{"title":"A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering","authors":"Gürhan Özkayar ,&nbsp;Yağmur Demircan Yalçın ,&nbsp;Ebru Özgür ,&nbsp;Ufuk Gündüz ,&nbsp;Haluk Külah","doi":"10.1016/j.protcy.2017.04.076","DOIUrl":"10.1016/j.protcy.2017.04.076","url":null,"abstract":"<div><p>In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30<!--> <!-->μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80528315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Label-free Impedimetric Immunosensors for Liver Cancer Stem Cells 肝癌干细胞无标记阻抗免疫传感器
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.119
Shimaa Eissa, Raja Chinnappan, M. Zourob
{"title":"Label-free Impedimetric Immunosensors for Liver Cancer Stem Cells","authors":"Shimaa Eissa, Raja Chinnappan, M. Zourob","doi":"10.1016/J.PROTCY.2017.04.119","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.119","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81614682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Electrochemical Characterization of Vitreous Humor 玻璃体幽默的电化学表征
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.124
Tjerignimin Silue, Saugandhika Mannikanti, N. Peixoto
{"title":"Electrochemical Characterization of Vitreous Humor","authors":"Tjerignimin Silue, Saugandhika Mannikanti, N. Peixoto","doi":"10.1016/J.PROTCY.2017.04.124","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.124","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81617747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform 自动化电化学酶联免疫吸附试验平台对生物威胁因子的多重检测
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.045
Christopher Pöhlmann , Laurent Bellanger , Michal Drevinek , Thomas Elßner
{"title":"Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform","authors":"Christopher Pöhlmann ,&nbsp;Laurent Bellanger ,&nbsp;Michal Drevinek ,&nbsp;Thomas Elßner","doi":"10.1016/j.protcy.2017.04.045","DOIUrl":"10.1016/j.protcy.2017.04.045","url":null,"abstract":"<div><p>Modern threats of bioterrorism force the need to immediately identify biothreat agents. Here, we present an electrochemical detection platform for identification of six biothreat agents in parallel within approx. 20 minutes in an automated procedure. The detection platform is permanently housed in a robust, lightweight suitcase offering a fully deconable housing. Limits of detection are in the range of 10<sup>3</sup> - 10<sup>5</sup> colony forming units per mL or 10<sup>4</sup> - 10<sup>5</sup> plaque forming units per mL for bacterial or viral agents, respectively. These results demonstrate the potential of electrochemical biochips for parallel and sensitive on-site detection of biothreat agents.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85821293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Shelf Life of Enzymatic Electrochemical Sensors 酶电化学传感器的保质期
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.126
P. Panjan, E. Ohtonen, P. Tervo, V. Virtanen, A. Sesay
{"title":"Shelf Life of Enzymatic Electrochemical Sensors","authors":"P. Panjan, E. Ohtonen, P. Tervo, V. Virtanen, A. Sesay","doi":"10.1016/J.PROTCY.2017.04.126","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.126","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84011637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Microfluidic Biochip for Studying Cellular Response to Non-homogeneous DC Electric Fields 研究细胞对非均匀直流电场响应的微流控生物芯片
Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.107
M. Río, Sharanya Bola, R. Funk, G. Gerlach
{"title":"Microfluidic Biochip for Studying Cellular Response to Non-homogeneous DC Electric Fields","authors":"M. Río, Sharanya Bola, R. Funk, G. Gerlach","doi":"10.1016/J.PROTCY.2017.04.107","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.107","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72785788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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