Ali Ghamin Al-Rubaye , Alexei Nabok , Anna Tsargorodska
{"title":"LSPR Biosensor Based on Nanostructured Gold Films: Detection of Mycotoxins","authors":"Ali Ghamin Al-Rubaye , Alexei Nabok , Anna Tsargorodska","doi":"10.1016/j.protcy.2017.04.057","DOIUrl":null,"url":null,"abstract":"<div><p>This work focuses on the development of optical sensors based on the LSPR phenomenon in nano-structured gold films suitable for detection of mycotoxins. A simple technology of annealing thin gold films was utilized for the formation of gold nano-islands exhibiting the LSPR effect. The morphology of gold nano-structures produced was studied with SEM and AFM while their optical properties were analysed with UV-vis absorption spectroscopy and spectroscopic ellipsometry (SE). A blue spectral shift of LSPR band caused by changes in the refractive index of medium constitutes the main principle of LSPR sensing. Bio-sensing tests were attempted using SE in total internal reflection mode (TIRE); a noticeable spectral shift was recorded on course of immune binding of aflatoxin B1 to specific antibodies immobilized on the surface of gold.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.057","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212017317300580","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
This work focuses on the development of optical sensors based on the LSPR phenomenon in nano-structured gold films suitable for detection of mycotoxins. A simple technology of annealing thin gold films was utilized for the formation of gold nano-islands exhibiting the LSPR effect. The morphology of gold nano-structures produced was studied with SEM and AFM while their optical properties were analysed with UV-vis absorption spectroscopy and spectroscopic ellipsometry (SE). A blue spectral shift of LSPR band caused by changes in the refractive index of medium constitutes the main principle of LSPR sensing. Bio-sensing tests were attempted using SE in total internal reflection mode (TIRE); a noticeable spectral shift was recorded on course of immune binding of aflatoxin B1 to specific antibodies immobilized on the surface of gold.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
