Christopher Pöhlmann , Laurent Bellanger , Michal Drevinek , Thomas Elßner
{"title":"自动化电化学酶联免疫吸附试验平台对生物威胁因子的多重检测","authors":"Christopher Pöhlmann , Laurent Bellanger , Michal Drevinek , Thomas Elßner","doi":"10.1016/j.protcy.2017.04.045","DOIUrl":null,"url":null,"abstract":"<div><p>Modern threats of bioterrorism force the need to immediately identify biothreat agents. Here, we present an electrochemical detection platform for identification of six biothreat agents in parallel within approx. 20 minutes in an automated procedure. The detection platform is permanently housed in a robust, lightweight suitcase offering a fully deconable housing. Limits of detection are in the range of 10<sup>3</sup> - 10<sup>5</sup> colony forming units per mL or 10<sup>4</sup> - 10<sup>5</sup> plaque forming units per mL for bacterial or viral agents, respectively. These results demonstrate the potential of electrochemical biochips for parallel and sensitive on-site detection of biothreat agents.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.045","citationCount":"5","resultStr":"{\"title\":\"Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform\",\"authors\":\"Christopher Pöhlmann , Laurent Bellanger , Michal Drevinek , Thomas Elßner\",\"doi\":\"10.1016/j.protcy.2017.04.045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Modern threats of bioterrorism force the need to immediately identify biothreat agents. Here, we present an electrochemical detection platform for identification of six biothreat agents in parallel within approx. 20 minutes in an automated procedure. The detection platform is permanently housed in a robust, lightweight suitcase offering a fully deconable housing. Limits of detection are in the range of 10<sup>3</sup> - 10<sup>5</sup> colony forming units per mL or 10<sup>4</sup> - 10<sup>5</sup> plaque forming units per mL for bacterial or viral agents, respectively. These results demonstrate the potential of electrochemical biochips for parallel and sensitive on-site detection of biothreat agents.</p></div>\",\"PeriodicalId\":101042,\"journal\":{\"name\":\"Procedia Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.045\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Procedia Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212017317300464\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212017317300464","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform
Modern threats of bioterrorism force the need to immediately identify biothreat agents. Here, we present an electrochemical detection platform for identification of six biothreat agents in parallel within approx. 20 minutes in an automated procedure. The detection platform is permanently housed in a robust, lightweight suitcase offering a fully deconable housing. Limits of detection are in the range of 103 - 105 colony forming units per mL or 104 - 105 plaque forming units per mL for bacterial or viral agents, respectively. These results demonstrate the potential of electrochemical biochips for parallel and sensitive on-site detection of biothreat agents.
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