Mucin 4 Immunosensor Based on p-aminophenylacetic Acid Grafting on Carbon Electrodes as Immobilization Platform

Oana Hosu, Mihaela Tertiş, Gheorghe Melinte, Robert Săndulescu, Cecilia Cristea
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引用次数: 1

Abstract

A simple impedimetric label-free immunosensor was developed for the specific and sensitive detection of mucin 4 (MUC 4) protein by using graphite based screen printed electrodes modified with an aryl diazonium salt or compound (p- aminophenylacetic acid) for the immobilization of antibody anti-MUC4 via amidic bond. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used in order to characterize and optimize the electrografting process. The parameters involved in each step of the immunosensor design were optimized. The performance of the immunoassay in terms of sensitivity, reproducibility and selectivity was studied.

基于对氨基苯基乙酸接枝碳电极作为固定平台的粘蛋白4免疫传感器
采用芳基重氮盐或化合物(对氨基苯基乙酸)修饰石墨基丝网印刷电极,通过酰胺键固定抗muc4抗体,研制了一种简便的无阻抗标记免疫传感器,用于muc4蛋白的特异性和敏感性检测。利用电化学阻抗谱(EIS)和循环伏安法(CV)对电接枝工艺进行了表征和优化。对免疫传感器设计各步骤所涉及的参数进行了优化。从灵敏度、重现性和选择性等方面研究了该免疫分析法的性能。
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