Procedia Technology最新文献_第8页

Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles 磁性纳米颗粒快速检测前列腺特异性抗原生物标志物

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.054

Ghadeer Suaifan , Mohammed Zourob

{"title":"Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles","authors":"Ghadeer Suaifan , Mohammed Zourob","doi":"10.1016/j.protcy.2017.04.054","DOIUrl":"10.1016/j.protcy.2017.04.054","url":null,"abstract":"<div><p>Prostate carcinoma is the most common leading cause of cancer-related deaths in men, aside from lung cancer. To date, proteolytically-active prostate specific antigen (PSA) serine protease is a useful diagnostic serological marker for the early diagnosis and monitoring of prostate cancer. In the past years, researchers executed a lot of research to increase the utility and applicability of PSA detection methods. However, these methods were usually frustrated by limitation in sensitivity, specificity, times constrains and ease of on-site application. Thus, there is a need for novel direct and highly sensitive PSA detection methods. This work showed the ability of the developed electrochemical and optical (Surface Plasmin Resonance (SPR) and colorimetric) biosensors to detect PSA with high sensitivity and specificity and within a short timing. Moreover, these novel approaches can be implemented in other miniaturized configurations such as screen printed electrode and paper-based low-cost point-of-care biosensor due to the elimination of washing and blocking steps as well as the amplification and labelling procedure.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 122-125"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80473666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Inhibition Biosensor Based on DC and AC Electrical Measurements of Bacteria Samples 基于细菌样品直流和交流电测量的抑制生物传感器

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.056

H. Abu-Ali , A. Nabok , T. Smith , M. Al-Shanawa

引用次数: 5

Investigation of Temperature Dependency on the Propulsion of Disk-like Nanoswimmers 盘状纳米游泳器推进的温度依赖性研究

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.023

Liangxing Hu , Nan Wang , Jianmin Miao , Gerhard Grüber

{"title":"Investigation of Temperature Dependency on the Propulsion of Disk-like Nanoswimmers","authors":"Liangxing Hu , Nan Wang , Jianmin Miao , Gerhard Grüber","doi":"10.1016/j.protcy.2017.04.023","DOIUrl":"10.1016/j.protcy.2017.04.023","url":null,"abstract":"<div><p>Flight vehicles can soar around Earth via ejecting the combusted gases in the space and bio-molecular motor proteins possess the ability to walk along the tracts through hydrolyzing adenosine triphosphate (ATP) in cells. Inspired by flight vehicles and naturally occurring bio-molecular protein motors, miniaturized disk-like nanoswimmers are proposed, which are composed of three different metals: gold (Au), nickel (Ni), and platinum (Pt). The proposed nanoswimmers are fabricated via a layer-by-layer deposition method base on nano-electro-mechanical systems (NEMS) technology, whereby Pt functions as the chemical catalyst for the decomposition of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) to produce oxygen (O<sub>2</sub>) bubbles detaching from its surface, which in turn generate recoil force to thrust nanoswimmers propelling forward. Herein, bubble propulsion mechanism originating from momentum change of a Au-Ni-Pt nanoswimmer-O<sub>2</sub> bubble integral system is proposed to investigate the propulsion of nanoswimmers. Experiments are mainly focused on characterizing the propulsion of nanoswimmers in diluted H<sub>2</sub>O<sub>2</sub> by changing the temperature of the solution. Results show that Au-Ni-Pt nanoswimmers are able to propel forward while the generated O<sub>2</sub> bubbles are detached from the Pt-surface. The speeds of nanoswimmers are increased with the increment of temperature varying from 7°C to 57°C. It is concluded that the propulsion of nanoswimmers is temperature-dependent.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 48-50"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76916022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Magnesium Zinc Oxide Nanostructure-modified Quartz Crystal Microbalance for Dynamic Monitoring of Antibiotic Effects and Antimicrobial Resistance 氧化镁锌纳米结构修饰石英晶体微天平用于抗生素效应和耐药性动态监测

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.022

Pavel Ivanoff Reyes , Keyang Yang , Andrew Zheng , Rui Li , Guangyuan Li , Yicheng Lu , Chi Kwan Tsang , Steven X.F. Zheng

{"title":"Magnesium Zinc Oxide Nanostructure-modified Quartz Crystal Microbalance for Dynamic Monitoring of Antibiotic Effects and Antimicrobial Resistance","authors":"Pavel Ivanoff Reyes , Keyang Yang , Andrew Zheng , Rui Li , Guangyuan Li , Yicheng Lu , Chi Kwan Tsang , Steven X.F. Zheng","doi":"10.1016/j.protcy.2017.04.022","DOIUrl":"10.1016/j.protcy.2017.04.022","url":null,"abstract":"<div><p>We demonstrate a magnesium zinc oxide (MZO) nanostructure-modified quartz crystal microbalance (MZO<sub>nano</sub>-QCM) biosensor to dynamically monitor antimicrobial effects on <em>E. coli</em> and <em>S. cerevisiae.</em> The MZO nanostructures were grown on the top electrode of a standard QCM using metal-organic chemical-vapor deposition (MOCVD). The MZO nanostructures are utilized as the sensing material due to their multifunctionality, biocompatibility, and very large effective sensing surface. The MZO surface-wettability and morphology are controlled, offering high-sensitivity to various biological/biochemical species. The MZO<sub>nano</sub>-QCM was applied to detect the effects of ampicillin and tetracycline on sensitive and resistant strains of <em>E.coli</em>, as well as effects of amphotericin-B and miconazole on <em>S. cerevisiae</em> through the device's time-dependent frequency shift and motional resistance.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 46-47"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87140412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Voltammetric DNA Biosensor using Gold Electrode Modified by Self Assembled Monolayer of Thiol for Detection of Mycobacterium Tuberculosis 自组装硫醇单层修饰金电极的伏安DNA生物传感器检测结核分枝杆菌

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.034

Shabarni Gaffar, Ratna Nurmalasari, Yohan, Yeni W. Hartati

{"title":"Voltammetric DNA Biosensor using Gold Electrode Modified by Self Assembled Monolayer of Thiol for Detection of Mycobacterium Tuberculosis","authors":"Shabarni Gaffar, Ratna Nurmalasari, Yohan, Yeni W. Hartati","doi":"10.1016/j.protcy.2017.04.034","DOIUrl":"10.1016/j.protcy.2017.04.034","url":null,"abstract":"<div><p><em>Mycobacterium tuberculosis</em> is an infectious agent that causes tuberculosis (TB). TB is one of the major causes of death worldwide, mainly in the developing country. Early and rapid diagnosis of TB will be of great help to isolate the patients and control the disease. The aim of this research is to detect <em>Mycobacterium tuberculosis</em> using voltammetric DNA biosensor by using gold electrode modified by self assembled monolayer with thiol. Single-stranded probe DNA was immobilized on the surface of self assembled monolayer gold electrode with the assistance of cysteamine and glutaraldehyde, which was further used to hybridize with the target DNA sequence and non-complementary target sequence. Differential Pulse Voltammetry (DPV) was used to study the immobilization of DNA probe and hybridization with the target DNA. The hybridization reaction on the gold electrode surface was detected by monitoring a guanine oxidation signal at +0.2 Volt. Voltammetric DNA biosensor using gold electrode modified with thiol can be used to determine hybridization between probe DNA and target DNA sequence of <em>M. tuberculosis</em> with sensitivity value is 0.5175 for target DNA in concentration range 0- 30 μg/mL; detection limit is 2.7046 μg/mL and quantification limit is 9.0155 μg/mL, accuracy is 99.22%, precision 99.86%</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 74-80"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83722916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Rapid Prototyping of a Low-cost Graphene-based Impedimetric Biosensor 低成本石墨烯阻抗生物传感器的快速原型设计

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.116

S. Popescu, C. Dale, N. Keegan, B. Ghosh, R. Kaner, J. Hedley

引用次数: 2

Development of Rapid Immuno-based Nanosensors for the Detection of Pathogenic Bacteria in Poultry Processing Plants 家禽加工厂致病菌快速免疫纳米传感器的研制

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.012

Saleh Alamer , Raja Chinnappan , Mohammed Zourob

{"title":"Development of Rapid Immuno-based Nanosensors for the Detection of Pathogenic Bacteria in Poultry Processing Plants","authors":"Saleh Alamer , Raja Chinnappan , Mohammed Zourob","doi":"10.1016/j.protcy.2017.04.012","DOIUrl":"10.1016/j.protcy.2017.04.012","url":null,"abstract":"<div><p>Foodborne diseases is a major public health concern and costs billions of dollars losses every year. For example, 93.8 million cases of gastroenteritis reported in Europe, United States, South America and Asia. Different methods depending on various scientific principles are used for detection of pathogenic bacteria relating foodborne diseases, conventional methods including culture-depending methods, microscopic, PCR, serological and biochemical methods are still used but majority of this methods suffers from a number of disadvantages includes long time-analysis, high cost, limited sensitivity and lab-based. Therefore, there is an urgent need for development of rapid screening assays for field applications with high sensitivity. In this study, we developed colorimetric immuno-sensor was developed and evaluated as a novel rapid detection nanosensing platform for the detection of foodborne pathogens such as Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus Aureus and Campylobacter Jejuni. The sensing platform composed of cotton swab and nanoparticles. Cotton swaps were functionalized with general or specific recognition receptors for collecting and pre-concentration of bacteria from the surfaces. Then the cotton swab was dipped in a developing solution to indicate the presence of specific bacteria. The developing solution has a cocktail of different coloured nanospheres conjugated with different recognition receptors for the various bacteria analytes was used to generate the different colours. The nanosensor was used for testing the bacteria on different surfaces mimicking the poultry processing units such as glass slides, stainless steel and chicken meat.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 23-26"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75756819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A High Throughput Lab-On-A-Chip System for Label Free Quantification of Breast Cancer Cells under Continuous Flow 一种用于连续流下乳腺癌细胞无标记定量的高通量芯片实验室系统

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.028

M.K. Aslan , Y. Demircan Yalcin , E. Ozgur , U. Gunduz , S. Eminoglu , H. Kulah , T. Akin

引用次数: 4

Functionalization of Gold-plasmonic Devices for Protein Capture 用于蛋白质捕获的金等离子体器件的功能化

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.071

E. Battista , P.L. Scognamiglio , G. Das , G. Manzo , F. Causa , E. Di Fabrizio , P.A. Netti

引用次数: 1

Highly Scalable Real Time Epilepsy Diagnosis Architecture Via Phase Correlation 基于相位相关的高度可扩展的实时癫痫诊断架构

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI: 10.1016/j.protcy.2017.04.026

James Brian Romaine, Manuel Delgado-Restituto, Ángel Rodríguez-Vázquez

引用次数: 0