Procedia Technology最新文献_第7页

Collision-based Electrochemistry for Investigation of Direct Electron Transfer of a Single Enzyme Molecule 基于碰撞电化学的单酶分子直接电子转移研究

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.101

Alina Sekretaryova, M. Vagin, A. Turner, M. Eriksson

引用次数: 0

Fabrication of 3-dimensional Cross-linked Redox Enzyme/Nanomaterials 三维交联氧化还原酶/纳米材料的制备

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.085

A.K.M. Kafi , N.S. Azmi , Mashitah M. Yusoff , Maxwell J. Crossley

引用次数: 0

Gold Nanostructuration in Paper-based Electrodes 纸基电极中的金纳米结构

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.058

E. Nunez-Bajo, M.C. Blanco-Lopez, A. Costa-García, M.T. Fernández-Abedul

引用次数: 2

System for Mobile Monitoring of Vital Functions and Environmental Context 生命功能和环境环境移动监测系统

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.068

Roman Trobec , Viktor Avbelj , Uroš Stanič , Matjaž Depolli , Aleksandra Rashkovska , Ivan Tomašič , Tomaž Krištofelc , Klemen Bregar , Gregor Kosec

引用次数: 2

A Nanoporous Alumina Membrane Based Impedance Biosensor for Histamine Detection with Magnetic Nanoparticles Separation and Amplification 基于纳米孔氧化铝膜的磁性纳米颗粒分离和扩增阻抗生物传感器用于组胺检测

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.051

W.W. Ye , Y.T. Ding , Y. Sun , F. Tian , M. Yang

{"title":"A Nanoporous Alumina Membrane Based Impedance Biosensor for Histamine Detection with Magnetic Nanoparticles Separation and Amplification","authors":"W.W. Ye , Y.T. Ding , Y. Sun , F. Tian , M. Yang","doi":"10.1016/j.protcy.2017.04.051","DOIUrl":"10.1016/j.protcy.2017.04.051","url":null,"abstract":"<div><p>Seafood, especially red fish is rich in histidine, which is essential for infants and adults. With fish freshness decline or spoilage, enzyme catalysis or microorganisms cause decarbonylation of histidine to form histamine, which threatens human body by dietary and affects allergic and inflammatory reaction. Current histamine assay needs complex operation, numerous steps, and time-consuming. In this study, a functionalized nanoporous alumina membrane was used to construct a rapid and highly sensitive impedance biosensor with magnetic nanoparticles (MNPs) for target molecule pre-concentration and separation. When the functionalized MNPs accumulated histamine, they were separated by magnetism from samples and added to the anti-histamine antibody modified nanoporous alumina membrane causing blocking effect in the nanopores by immune reaction. Impedance increased as histamine concentrations increased from 1 μM to 40 mM. The limit of detection was as low as several micromolar. The biosensor provides a novel, highly sensitive and specific sensing mechanism and constructs a new technology and method for justifying seafood freshness to prevent toxic reaction in human body. It is a new approach for food quality safety control.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73046997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

D-dimer Quantification from Autologous Red Blood Cells Agglutination by a Lens-free Imaging Device 用无透镜成像装置定量测定自体红细胞凝集d -二聚体

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.073

M. Huet , M. Cubizolles , P. Pouteau , V. Poher , A. Buhot

引用次数: 1

Inhibition Biosensor Based on DC and AC Electrical Measurements of Bacteria Samples 基于细菌样品直流和交流电测量的抑制生物传感器

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.056

H. Abu-Ali , A. Nabok , T. Smith , M. Al-Shanawa

引用次数: 5

Single Interdigital Transducer as Surface Acoustic Wave Impedance Sensor 单数字换能器作为表面声波阻抗传感器

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.032

V.H. Nguyen , S. Richert , H. Park , A. Böker , U. Schnakenberg

引用次数: 2

A Novel Quantum Dot Fluorescence Immunosensor Based on Magnetic Beads and Portable Flow Cytometry for Detection of Okadaic Acid 基于磁珠和便携式流式细胞术的量子点荧光免疫传感器检测冈田酸

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.090

Yuxiang Pan , Jie Zhou , Kaiqi Su , Ning Hu , Ping Wang

{"title":"A Novel Quantum Dot Fluorescence Immunosensor Based on Magnetic Beads and Portable Flow Cytometry for Detection of Okadaic Acid","authors":"Yuxiang Pan , Jie Zhou , Kaiqi Su , Ning Hu , Ping Wang","doi":"10.1016/j.protcy.2017.04.090","DOIUrl":"10.1016/j.protcy.2017.04.090","url":null,"abstract":"<div><p>Okadaic acid (OA) is a representative diarrhetic shellfish poisoning toxin which is highly toxic and carcinogenic to human, and rich in polluted shellfish. In this work, a novel and high sensitive fluorescence immunosensor based on magnetic beads (MBs) and quantum dots (QDs) had been developed for OA detection. Carboxylic acid modified MBs were used as supporter to immobilize the OA-BSA, competed with the OA in the sample solution to bind with the anti-OA monoclonal antibody (OA-MAb). The CdTe QDs (EX600 nm) labeled IgG was served as secondary antibody to perform fluorescence detection. A portable flow cytometry, Moxi Flow was applied for OA on-site quantification. The results showed that the OA concentration was inversely proportional to the QDs fluorescence intensity. The limit of detection (LOD) was 0.05 μg/L with a linear range of 0.2–20 μg/L for OA detection, which was far lower than traditional id-ELISA strategy. Moreover, OA detection for the real sample could be completed within 1 hour. The matrix effect and the recovery rate were also assessed during real sample measurement, showing a high recovery.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74269865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles 磁性纳米颗粒快速检测前列腺特异性抗原生物标志物

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.054

Ghadeer Suaifan , Mohammed Zourob

{"title":"Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles","authors":"Ghadeer Suaifan , Mohammed Zourob","doi":"10.1016/j.protcy.2017.04.054","DOIUrl":"10.1016/j.protcy.2017.04.054","url":null,"abstract":"<div><p>Prostate carcinoma is the most common leading cause of cancer-related deaths in men, aside from lung cancer. To date, proteolytically-active prostate specific antigen (PSA) serine protease is a useful diagnostic serological marker for the early diagnosis and monitoring of prostate cancer. In the past years, researchers executed a lot of research to increase the utility and applicability of PSA detection methods. However, these methods were usually frustrated by limitation in sensitivity, specificity, times constrains and ease of on-site application. Thus, there is a need for novel direct and highly sensitive PSA detection methods. This work showed the ability of the developed electrochemical and optical (Surface Plasmin Resonance (SPR) and colorimetric) biosensors to detect PSA with high sensitivity and specificity and within a short timing. Moreover, these novel approaches can be implemented in other miniaturized configurations such as screen printed electrode and paper-based low-cost point-of-care biosensor due to the elimination of washing and blocking steps as well as the amplification and labelling procedure.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80473666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9