Procedia Technology最新文献

{"title":"System for Mobile Monitoring of Vital Functions and Environmental Context","authors":"Roman Trobec ,&nbsp;Viktor Avbelj ,&nbsp;Uroš Stanič ,&nbsp;Matjaž Depolli ,&nbsp;Aleksandra Rashkovska ,&nbsp;Ivan Tomašič ,&nbsp;Tomaž Krištofelc ,&nbsp;Klemen Bregar ,&nbsp;Gregor Kosec","doi":"10.1016/j.protcy.2017.04.068","DOIUrl":"10.1016/j.protcy.2017.04.068","url":null,"abstract":"<div><p>The use of ICT in the health care system is aimed to increase efficacy and decrease costs. We have designed a system and services for mobile monitoring of vital physiological parameters and environmental context. The system is based on a small multi-functional wireless body sensor for simultaneous measurement of ECG, temperature, heart rate, respiration rate, and movement. The sensor is wirelessly connected through Bluetooth Smart to a Smartphone. When necessary, the measurements are transferred to a server and can be accessed by authorized personnel. The contribution of the system to improved quality and efficiency of medical care is validated and estimated through several pilot studies.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 157-158"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88418055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Selective Voltammetric Sensor Based on Molecularly Imprinted Polymer and Carbon Nanotubes to Determine the Dicloran Pesticide in Biological and Environmental Samples","authors":"Seyed Jamaleddin Shahtaheri ,&nbsp;Farnoush Faridbod ,&nbsp;Monireh Khadem","doi":"10.1016/j.protcy.2017.04.041","DOIUrl":"10.1016/j.protcy.2017.04.041","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;p&gt;Despite of increasing the food production by application of pesticides, the wide use of them can lead to environmental pollution and their residues in food. Due to the increasing application of pesticides, reliable and accurate analytical methods are necessary to analyze different occupational and environmental samples like air, water, soil, as well as also food containing these compounds. There are some traditional techniques to determine the pesticides such as liquid chromatography and gas chromatography with electron capture detection. The mentioned techniques are very expensive and a well-equipped laboratory and well-trained analysis operators are required. A sample pre-treatment step is needed to determine the analytes at ppb levels. Therefore, in the last few years many sensitive, selective, and accurate methods have been developed to determine the trace toxic species like pesticides. Electrochemical sensors are the appropriate and interested devices to monitor the trace and even ultra-trace pesticides. Molecular imprinted polymers (MIPs) can be used as recognition elements or modifying agents in sensors structure to increase their selectivity and improve their response. Recently, modified electrode by different modifying agents like MIPs and various nano structures are being used for quantification of analytes because of their interesting advantages. Modified electrodes may be used in combination with different electrochemical techniques. The aim of this study was to synthesize a molecularly imprinted polymer for dicloran for first time and then to apply it as a recognition element in the nano-composite carbon paste electrode for selective and sensitive electrochemical determining the dicloran pesticide in environmental and biological samples.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;Multi-walls carbon nanotubes (MWCNTs) and a molecularly imprinted polymer (MIP) were used as the modifiers in senor composition. A dicloran selective MIP and a non-imprinted polymer (NIP) were synthesized and applied in the carbon paste electrode. To prepare the bare carbon paste electrode (CP), graphite, MWCNT and paraffin oil were mixed. The MIP-CP and NIP-CP were prepared by mixing different percentages of graphite, MWCNT, paraffin oil, and MIP or NIP. This mixture was homogenized and final paste was packed into the end of an electrode body. After the optimization of electrode composition, it was used to extract the analyte in the sample and then was inserted in the electrochemical cell to determine the concentration of extracted analyte. Some parameters affecting the sensor response were optimized in the extraction and analysis steps, such as sample pH, electrolyte concentration and pH, stirring rate of analyte solution, as well as the instrumental parameters of square wave voltammetry (square wave amplitude and frequency, deposition potential and its exertion time, and electrolyte concentration).&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results and Discussi","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 96-97"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81623101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nanofluidic Mixing Device for High-throughput Fluorescence Sensing of Single Molecules","authors":"Klaus Mathwig ,&nbsp;Carel Fijen ,&nbsp;Mattia Fontana ,&nbsp;Serge G. Lemay ,&nbsp;Johannes Hohlbein","doi":"10.1016/j.protcy.2017.04.062","DOIUrl":"10.1016/j.protcy.2017.04.062","url":null,"abstract":"<div><p>We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 141-142"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82237164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of 3-dimensional Cross-linked Redox Enzyme/Nanomaterials","authors":"A.K.M. Kafi ,&nbsp;N.S. Azmi ,&nbsp;Mashitah M. Yusoff ,&nbsp;Maxwell J. Crossley","doi":"10.1016/j.protcy.2017.04.085","DOIUrl":"10.1016/j.protcy.2017.04.085","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 201-202"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84797560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Plasmon Resonance Immunoassay for Biotin Determination on a Home-made Instrument","authors":"Xing Chen,&nbsp;Lulu Zhang,&nbsp;Dafu Cui","doi":"10.1016/j.protcy.2017.04.037","DOIUrl":"10.1016/j.protcy.2017.04.037","url":null,"abstract":"<div><p>In this paper, a home-made SPR analytical system and a denaturalized bovine serum albumin (dBSA) SPR chip were used to detect biotin based on the method of an inhibition immunoassay. The experimental was compared with a commercial protocol. The conditions of regeneration were optimized. The dBSA SPR chip was fabricated by a self-assembling method and the derivative of biotin was immobilized on the dBSA SPR chip by using a on-line mode. A commercial instrument and a dextran-SPR-chip (CM5) were used to implement the same experiments.</p><p>Based on the experiments of immune reactions between biotin antibody and biotin derivative, the biotin antibody concentration was optimized at about 5 μg/mL in order to obtain highly sensitivity, which was used in the followed inhibition immunoassays. The lowest detection limit for biotin is 0.1 μg/mL with a detection range of 0.1-1000 μg/ml by using our home-made instrument and the dBSA-SPR-chip. The detection results of our instrument and dBSA SPR chip are comparable with the commercial protocol. Our home-made instrument and dBSA-SPR-chips can be also applied to detect other small molecules based on the indirect inhibition immunoassay.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 87-88"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88674477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic Concentration Gradient for Toxicity Studies of Lung Carcinoma Cells","authors":"Nuradawiyah Zaidon ,&nbsp;Ahmad Fairuzabadi Mohd Mansor ,&nbsp;Wing Cheung Mak ,&nbsp;Ahmad Faris Ismail ,&nbsp;Anis Nurashikin Nordin","doi":"10.1016/j.protcy.2017.04.067","DOIUrl":"10.1016/j.protcy.2017.04.067","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Cancer is a serious global health problem, which resulted in 8.2 million deaths in 2012 alone. Amongst different types of cancer, lung cancer is the most lethal and contributes 19.4% of cancer deaths. Better disease-free cancer survival rates have been reported when surgery is followed by systemic chemotherapy. Efficient treatment can be achieved through personalized chemotherapy dosage whereby optimum treatment is given to kill the cancer the side effects are minimized. Here, we present a microfluidic concentration gradient device for toxicity studies on lung cancer cell lines (A549). Automated drug dilution is achieved by simply tuning the flow rate and geometries of the microfluidics network. Sets of tree-like-concentration-generators were designed to achieve constant flow rate at each outlet by optimizing the channel lengths. Serpentine structures were placed in the middle in the middle and at each outlet channel to the design to improve mixing along the channel. The lengths of middle and outlet channels are varied from 1.5&lt;!--&gt; &lt;!--&gt;mm to 12&lt;!--&gt; &lt;!--&gt;mm to obtain sufficient mixing of two fluid flows. Theoretically, correlations between hydraulic flow and electrical circuit equations analogy were applied to ease the microfluidic design process. Later, 3D (dimensional) simulations using computational fluid dynamic (CFD)-based simulator, i.e. Ansys FLUENT were performed by implementing species transport method prior to fabrication. The simulation process helps to demonstrate the effect of varying channel length on the velocity magnitude and the concentration of the microfluidic structure. In addition, the simulation results allows us predict the fluid flow velocity that showed constant velocity magnitude at each outlet. Wider range of dilution can be achieved, when a higher number of outlets are added in a microfluidic design. Polydimethylsiloxane (PDMS) microchannels were fabricated on glass slide widths of 200&lt;!--&gt; &lt;!--&gt;μm and depths of 35&lt;!--&gt; &lt;!--&gt;μm using soft-lithography technique &lt;span&gt;[1]&lt;/span&gt;. The 3-outlet serpentine structure produced the best match between simulation and measurement results. The concentration profiles produce inside the channel is determined by the splitting ratio of the fluids at each branched and also depends on the number of the inlet and outlet in the tree-like network.&lt;/p&gt;&lt;p&gt;The gradient generator will be attached to an array of cell culture chambers with sensors that were previously developed for toxicity studies of lung cancer (A549) cell lines is shown in the Fig. 2. Cells cultured in the sensor will begin to attach and spread on the surface of the electrodes, restricting current flows from the electrodes to the surrounding media. In a confluent (all surface covered with cells) cell layer, current must travel through the intercellular space of the cell-cell and also the tight gap of the cell-electrode pairs to reach surrounding media. The more adhered the cells are with each other and with the elec","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 153-154"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87670041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Quantum Dot Fluorescence Immunosensor Based on Magnetic Beads and Portable Flow Cytometry for Detection of Okadaic Acid","authors":"Yuxiang Pan ,&nbsp;Jie Zhou ,&nbsp;Kaiqi Su ,&nbsp;Ning Hu ,&nbsp;Ping Wang","doi":"10.1016/j.protcy.2017.04.090","DOIUrl":"10.1016/j.protcy.2017.04.090","url":null,"abstract":"<div><p>Okadaic acid (OA) is a representative diarrhetic shellfish poisoning toxin which is highly toxic and carcinogenic to human, and rich in polluted shellfish. In this work, a novel and high sensitive fluorescence immunosensor based on magnetic beads (MBs) and quantum dots (QDs) had been developed for OA detection. Carboxylic acid modified MBs were used as supporter to immobilize the OA-BSA, competed with the OA in the sample solution to bind with the anti-OA monoclonal antibody (OA-MAb). The CdTe QDs (EX600 nm) labeled IgG was served as secondary antibody to perform fluorescence detection. A portable flow cytometry, Moxi Flow was applied for OA on-site quantification. The results showed that the OA concentration was inversely proportional to the QDs fluorescence intensity. The limit of detection (LOD) was 0.05 μg/L with a linear range of 0.2–20 μg/L for OA detection, which was far lower than traditional id-ELISA strategy. Moreover, OA detection for the real sample could be completed within 1 hour. The matrix effect and the recovery rate were also assessed during real sample measurement, showing a high recovery.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 214-216"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74269865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Non-invasive Biosensor Based on Electric Field Detection for Cardio-electrophysiology in Zebrafish Embryos","authors":"E. Rendon-Morales, R. Prance, H. Prance, R. Aviles-Espinosa","doi":"10.1016/J.PROTCY.2017.04.103","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.103","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"14 1","pages":"242-243"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90289279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small Molecule SPR Imaging Detection from Split Aptamer Microarrays","authors":"Feriel Melaine ,&nbsp;Yoann Roupioz ,&nbsp;Arnaud Buhot","doi":"10.1016/j.protcy.2017.04.004","DOIUrl":"10.1016/j.protcy.2017.04.004","url":null,"abstract":"<div><p>The small molecules detection remains a challenge for diagnostics. Due to their size, difficulties arise in finding selective and sensitive probes as well as developing appropriate detection format based on sandwich and/or signal amplification assays. In this study, we combine multiple strategies: a) the use of aptamer probes, short oligonucleotides strands selected in order to present strong selectivity for the small molecule target, b) the sequence engineering of split versions of the aptamer for a sandwich assay and c) the amplification of Surface Plasmon Resonance imaging (SPRi) signal by the use of gold nanoparticles (AuNPs). Combining those three strategies lead to state-of-the-art limit of detection (LOD = 50 nM) for the adenosine target.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 6-7"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87460398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single Interdigital Transducer as Surface Acoustic Wave Impedance Sensor","authors":"V.H. Nguyen ,&nbsp;S. Richert ,&nbsp;H. Park ,&nbsp;A. Böker ,&nbsp;U. Schnakenberg","doi":"10.1016/j.protcy.2017.04.032","DOIUrl":"10.1016/j.protcy.2017.04.032","url":null,"abstract":"<div><p>Surface acoustic wave (SAW) devices are well-known for gravimetric sensor applications. In biosensing applications, chemical- and biochemically evoked adsorption processes at surfaces are detected in liquid environments using delay-line or resonator sensor configurations, preferably in combination with appropriate microfluidic devices. In this paper, a novel SAW-based impedance sensor type is introduced which uses only one interdigital electrode transducer (IDT) simultaneously as SAW generator and sensor element. It is shown that the amplitude of the reflected S₁₁ signal directly depends on the input impedance of the SAW device. The input impedance is strongly influenced by mass adsorption which causes a characteristic and measurable impedance mismatch.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 70-71"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73216172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}