Procedia Technology最新文献_第6页

A Nanoporous Alumina Membrane Based Impedance Biosensor for Histamine Detection with Magnetic Nanoparticles Separation and Amplification 基于纳米孔氧化铝膜的磁性纳米颗粒分离和扩增阻抗生物传感器用于组胺检测

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.051

W.W. Ye , Y.T. Ding , Y. Sun , F. Tian , M. Yang

{"title":"A Nanoporous Alumina Membrane Based Impedance Biosensor for Histamine Detection with Magnetic Nanoparticles Separation and Amplification","authors":"W.W. Ye , Y.T. Ding , Y. Sun , F. Tian , M. Yang","doi":"10.1016/j.protcy.2017.04.051","DOIUrl":"10.1016/j.protcy.2017.04.051","url":null,"abstract":"<div><p>Seafood, especially red fish is rich in histidine, which is essential for infants and adults. With fish freshness decline or spoilage, enzyme catalysis or microorganisms cause decarbonylation of histidine to form histamine, which threatens human body by dietary and affects allergic and inflammatory reaction. Current histamine assay needs complex operation, numerous steps, and time-consuming. In this study, a functionalized nanoporous alumina membrane was used to construct a rapid and highly sensitive impedance biosensor with magnetic nanoparticles (MNPs) for target molecule pre-concentration and separation. When the functionalized MNPs accumulated histamine, they were separated by magnetism from samples and added to the anti-histamine antibody modified nanoporous alumina membrane causing blocking effect in the nanopores by immune reaction. Impedance increased as histamine concentrations increased from 1 μM to 40 mM. The limit of detection was as low as several micromolar. The biosensor provides a novel, highly sensitive and specific sensing mechanism and constructs a new technology and method for justifying seafood freshness to prevent toxic reaction in human body. It is a new approach for food quality safety control.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 116-117"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73046997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

D-dimer Quantification from Autologous Red Blood Cells Agglutination by a Lens-free Imaging Device 用无透镜成像装置定量测定自体红细胞凝集d -二聚体

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.073

M. Huet , M. Cubizolles , P. Pouteau , V. Poher , A. Buhot

引用次数: 1

Inhibition Biosensor Based on DC and AC Electrical Measurements of Bacteria Samples 基于细菌样品直流和交流电测量的抑制生物传感器

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.056

H. Abu-Ali , A. Nabok , T. Smith , M. Al-Shanawa

引用次数: 5

Single Interdigital Transducer as Surface Acoustic Wave Impedance Sensor 单数字换能器作为表面声波阻抗传感器

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.032

V.H. Nguyen , S. Richert , H. Park , A. Böker , U. Schnakenberg

引用次数: 2

A Novel Quantum Dot Fluorescence Immunosensor Based on Magnetic Beads and Portable Flow Cytometry for Detection of Okadaic Acid 基于磁珠和便携式流式细胞术的量子点荧光免疫传感器检测冈田酸

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.090

Yuxiang Pan , Jie Zhou , Kaiqi Su , Ning Hu , Ping Wang

{"title":"A Novel Quantum Dot Fluorescence Immunosensor Based on Magnetic Beads and Portable Flow Cytometry for Detection of Okadaic Acid","authors":"Yuxiang Pan , Jie Zhou , Kaiqi Su , Ning Hu , Ping Wang","doi":"10.1016/j.protcy.2017.04.090","DOIUrl":"10.1016/j.protcy.2017.04.090","url":null,"abstract":"<div><p>Okadaic acid (OA) is a representative diarrhetic shellfish poisoning toxin which is highly toxic and carcinogenic to human, and rich in polluted shellfish. In this work, a novel and high sensitive fluorescence immunosensor based on magnetic beads (MBs) and quantum dots (QDs) had been developed for OA detection. Carboxylic acid modified MBs were used as supporter to immobilize the OA-BSA, competed with the OA in the sample solution to bind with the anti-OA monoclonal antibody (OA-MAb). The CdTe QDs (EX600 nm) labeled IgG was served as secondary antibody to perform fluorescence detection. A portable flow cytometry, Moxi Flow was applied for OA on-site quantification. The results showed that the OA concentration was inversely proportional to the QDs fluorescence intensity. The limit of detection (LOD) was 0.05 μg/L with a linear range of 0.2–20 μg/L for OA detection, which was far lower than traditional id-ELISA strategy. Moreover, OA detection for the real sample could be completed within 1 hour. The matrix effect and the recovery rate were also assessed during real sample measurement, showing a high recovery.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 214-216"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74269865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles 磁性纳米颗粒快速检测前列腺特异性抗原生物标志物

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.054

Ghadeer Suaifan , Mohammed Zourob

{"title":"Rapid Detection of Prostate Specific Antigen Biomarker Using Magnetic-Nanoparticles","authors":"Ghadeer Suaifan , Mohammed Zourob","doi":"10.1016/j.protcy.2017.04.054","DOIUrl":"10.1016/j.protcy.2017.04.054","url":null,"abstract":"<div><p>Prostate carcinoma is the most common leading cause of cancer-related deaths in men, aside from lung cancer. To date, proteolytically-active prostate specific antigen (PSA) serine protease is a useful diagnostic serological marker for the early diagnosis and monitoring of prostate cancer. In the past years, researchers executed a lot of research to increase the utility and applicability of PSA detection methods. However, these methods were usually frustrated by limitation in sensitivity, specificity, times constrains and ease of on-site application. Thus, there is a need for novel direct and highly sensitive PSA detection methods. This work showed the ability of the developed electrochemical and optical (Surface Plasmin Resonance (SPR) and colorimetric) biosensors to detect PSA with high sensitivity and specificity and within a short timing. Moreover, these novel approaches can be implemented in other miniaturized configurations such as screen printed electrode and paper-based low-cost point-of-care biosensor due to the elimination of washing and blocking steps as well as the amplification and labelling procedure.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 122-125"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80473666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Magnesium Zinc Oxide Nanostructure-modified Quartz Crystal Microbalance for Dynamic Monitoring of Antibiotic Effects and Antimicrobial Resistance 氧化镁锌纳米结构修饰石英晶体微天平用于抗生素效应和耐药性动态监测

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.022

Pavel Ivanoff Reyes , Keyang Yang , Andrew Zheng , Rui Li , Guangyuan Li , Yicheng Lu , Chi Kwan Tsang , Steven X.F. Zheng

{"title":"Magnesium Zinc Oxide Nanostructure-modified Quartz Crystal Microbalance for Dynamic Monitoring of Antibiotic Effects and Antimicrobial Resistance","authors":"Pavel Ivanoff Reyes , Keyang Yang , Andrew Zheng , Rui Li , Guangyuan Li , Yicheng Lu , Chi Kwan Tsang , Steven X.F. Zheng","doi":"10.1016/j.protcy.2017.04.022","DOIUrl":"10.1016/j.protcy.2017.04.022","url":null,"abstract":"<div><p>We demonstrate a magnesium zinc oxide (MZO) nanostructure-modified quartz crystal microbalance (MZO<sub>nano</sub>-QCM) biosensor to dynamically monitor antimicrobial effects on <em>E. coli</em> and <em>S. cerevisiae.</em> The MZO nanostructures were grown on the top electrode of a standard QCM using metal-organic chemical-vapor deposition (MOCVD). The MZO nanostructures are utilized as the sensing material due to their multifunctionality, biocompatibility, and very large effective sensing surface. The MZO surface-wettability and morphology are controlled, offering high-sensitivity to various biological/biochemical species. The MZO<sub>nano</sub>-QCM was applied to detect the effects of ampicillin and tetracycline on sensitive and resistant strains of <em>E.coli</em>, as well as effects of amphotericin-B and miconazole on <em>S. cerevisiae</em> through the device's time-dependent frequency shift and motional resistance.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 46-47"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87140412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Novel Non-invasive Biosensor Based on Electric Field Detection for Cardio-electrophysiology in Zebrafish Embryos 基于电场检测的新型无创生物传感器在斑马鱼胚胎心电生理中的应用

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/J.PROTCY.2017.04.103

E. Rendon-Morales, R. Prance, H. Prance, R. Aviles-Espinosa

引用次数: 1

Small Molecule SPR Imaging Detection from Split Aptamer Microarrays 分裂适体微阵列小分子SPR成像检测

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.004

Feriel Melaine , Yoann Roupioz , Arnaud Buhot

引用次数: 3

Investigation of Temperature Dependency on the Propulsion of Disk-like Nanoswimmers 盘状纳米游泳器推进的温度依赖性研究

Procedia Technology Pub Date : 2017-01-01 DOI: 10.1016/j.protcy.2017.04.023

Liangxing Hu , Nan Wang , Jianmin Miao , Gerhard Grüber

{"title":"Investigation of Temperature Dependency on the Propulsion of Disk-like Nanoswimmers","authors":"Liangxing Hu , Nan Wang , Jianmin Miao , Gerhard Grüber","doi":"10.1016/j.protcy.2017.04.023","DOIUrl":"10.1016/j.protcy.2017.04.023","url":null,"abstract":"<div><p>Flight vehicles can soar around Earth via ejecting the combusted gases in the space and bio-molecular motor proteins possess the ability to walk along the tracts through hydrolyzing adenosine triphosphate (ATP) in cells. Inspired by flight vehicles and naturally occurring bio-molecular protein motors, miniaturized disk-like nanoswimmers are proposed, which are composed of three different metals: gold (Au), nickel (Ni), and platinum (Pt). The proposed nanoswimmers are fabricated via a layer-by-layer deposition method base on nano-electro-mechanical systems (NEMS) technology, whereby Pt functions as the chemical catalyst for the decomposition of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) to produce oxygen (O<sub>2</sub>) bubbles detaching from its surface, which in turn generate recoil force to thrust nanoswimmers propelling forward. Herein, bubble propulsion mechanism originating from momentum change of a Au-Ni-Pt nanoswimmer-O<sub>2</sub> bubble integral system is proposed to investigate the propulsion of nanoswimmers. Experiments are mainly focused on characterizing the propulsion of nanoswimmers in diluted H<sub>2</sub>O<sub>2</sub> by changing the temperature of the solution. Results show that Au-Ni-Pt nanoswimmers are able to propel forward while the generated O<sub>2</sub> bubbles are detached from the Pt-surface. The speeds of nanoswimmers are increased with the increment of temperature varying from 7°C to 57°C. It is concluded that the propulsion of nanoswimmers is temperature-dependent.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 48-50"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76916022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7