Electrocatalytic Evaluation of a Horseradish Peroxidase Biosensor Based on a Novel Bi-Ag Bimetallic Nanocomposite

Procedia Technology Pub Date : 2017-01-01 Epub Date: 2017-07-13 DOI:10.1016/j.protcy.2017.04.077

Charlton van der Horst , Bongiwe Silwana , Emmanuel Iwuoha , Vernon Somerset

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引用次数: 6

Abstract

The present work illustrates the construction and application of bismuth-silver bimetallic nanoparticles (Bi-AgNPs) in biosensor construction for the detection of peroxide (H₂O₂). The biosensor was constructed by drop-casting of horseradish peroxidase onto the Bi-AgNPs/GCE sensor. Impedance analysis revealed that the R_ct value for the bare GCE obtained was 2.548 × 10³ Ω and increased to 5.768 × 10³ Ω for the HRP/Bi-AgNPs/GCE biosensor. This increased value of R_ct indicated hindrance to the electron transfer, providing evidence for the immobilization of insulating HRP on the Bi-AgNPs/GCE surface. The HRP/Bi-AgNPs/GCE biosensor was successfully applied for sensitive detection of H₂O₂ by differential pulse voltammetry. Well defined peaks were observed with a low linearity range from 0.02 × 10⁻⁶ to 1.0 × 10⁻⁶ M (R² = 0.991), with a limit of detection of 0.06 μM. The results obtained for the HRP/Bi-AgNPs/GCE biosensor compared well within the range of similar biosensors reported.

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新型Bi-Ag双金属纳米复合材料对辣根过氧化物酶生物传感器的电催化评价

本文阐述了铋银双金属纳米粒子(Bi-AgNPs)在过氧化氢(H2O2)检测生物传感器中的构建和应用。将辣根过氧化物酶滴铸到Bi-AgNPs/GCE传感器上。阻抗分析表明，获得的裸GCE的Rct值为2.548 × 103 Ω， HRP/Bi-AgNPs/GCE生物传感器的Rct值增加到5.768 × 103 Ω。Rct值的增加表明电子转移受到阻碍，为在Bi-AgNPs/GCE表面固定化绝缘HRP提供了证据。HRP/Bi-AgNPs/GCE生物传感器成功应用于差分脉冲伏安法对H2O2的灵敏检测。在0.02 × 10−6 ~ 1.0 × 10−6 M的低线性范围内(R2 = 0.991)，检测限为0.06 μM。HRP/Bi-AgNPs/GCE生物传感器获得的结果与报道的类似生物传感器的范围相比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Procedia Technology

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