G. Martínez-García, L. Agüí, P. Yáñez-Sedeño, J.M. Pingarrón
{"title":"用石墨烯修饰电极作为抗体固定平台的多路电化学肥胖相关激素免疫传感器","authors":"G. Martínez-García, L. Agüí, P. Yáñez-Sedeño, J.M. Pingarrón","doi":"10.1016/j.protcy.2017.04.080","DOIUrl":null,"url":null,"abstract":"<div><p>An electrochemical immunosensor for the simultaneous determination of ghrelin (GHRL) and peptide YY (PYY) using dual screen-printed carbon electrodes modified with reduced graphene oxide (rGO) is presented. Diazonium salt of 4-aminobenzoic acid (4-ABA) was electrochemically grafted on the modified electrodes allowing covalent immobilization of antibodies. After competitive immunoassays using alkaline phosphatase labelled antigens, the affinity reactions were monitored by DPV upon addition of 1-naphthyl phosphate. Calibration plots showed linear current vs. log [hormone] ranges from 10<sup>-3</sup> to 100 ng/mL GHRL, and 10<sup>-4</sup> to10 ng/mL PYY. The usefulness of dual immunosensor was demonstrated by analysis of spiked human serum and saliva.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.080","citationCount":"7","resultStr":"{\"title\":\"Multiplexed Electrochemical Immunosensor for Obesity-related Hormones Using Grafted Graphene-modified Electrodes as Platforms for Antibodies Immobilization\",\"authors\":\"G. Martínez-García, L. Agüí, P. Yáñez-Sedeño, J.M. Pingarrón\",\"doi\":\"10.1016/j.protcy.2017.04.080\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An electrochemical immunosensor for the simultaneous determination of ghrelin (GHRL) and peptide YY (PYY) using dual screen-printed carbon electrodes modified with reduced graphene oxide (rGO) is presented. Diazonium salt of 4-aminobenzoic acid (4-ABA) was electrochemically grafted on the modified electrodes allowing covalent immobilization of antibodies. After competitive immunoassays using alkaline phosphatase labelled antigens, the affinity reactions were monitored by DPV upon addition of 1-naphthyl phosphate. Calibration plots showed linear current vs. log [hormone] ranges from 10<sup>-3</sup> to 100 ng/mL GHRL, and 10<sup>-4</sup> to10 ng/mL PYY. The usefulness of dual immunosensor was demonstrated by analysis of spiked human serum and saliva.</p></div>\",\"PeriodicalId\":101042,\"journal\":{\"name\":\"Procedia Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.080\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Procedia Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212017317300816\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212017317300816","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Multiplexed Electrochemical Immunosensor for Obesity-related Hormones Using Grafted Graphene-modified Electrodes as Platforms for Antibodies Immobilization
An electrochemical immunosensor for the simultaneous determination of ghrelin (GHRL) and peptide YY (PYY) using dual screen-printed carbon electrodes modified with reduced graphene oxide (rGO) is presented. Diazonium salt of 4-aminobenzoic acid (4-ABA) was electrochemically grafted on the modified electrodes allowing covalent immobilization of antibodies. After competitive immunoassays using alkaline phosphatase labelled antigens, the affinity reactions were monitored by DPV upon addition of 1-naphthyl phosphate. Calibration plots showed linear current vs. log [hormone] ranges from 10-3 to 100 ng/mL GHRL, and 10-4 to10 ng/mL PYY. The usefulness of dual immunosensor was demonstrated by analysis of spiked human serum and saliva.
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