Procedia Technology最新文献

{"title":"Development of Rapid and Low-cost Paper Based Sensing Platform for Bacterial Detection","authors":"Sahar Alhogail ,&nbsp;Ghadeer A.R.Y. Suaifan ,&nbsp;Mohammed Zourob","doi":"10.1016/j.protcy.2017.04.064","DOIUrl":"10.1016/j.protcy.2017.04.064","url":null,"abstract":"<div><p>The development of highly sensitive and specific colorimetric biosensor for pathogens spot detection is currently researchers challenge. This study illustrates the design and development of specific peptide probe for the detection of specific proteases secreted from pathogens. These proteases precisely cleaved specific peptide substrate labeled with nano-magnetic beads and attached to gold sensing surface.</p><p>The superiority of this approach is based on its low-cost, simplicity being colorimetric, can be run by unskilled personnel and can be applied to real samples (without the need for isolation or purification) in short time (1-5<!--> <!-->minute). Furthermore the test can be printed/fabricated on personal healthcare.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 146-148"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87921169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free Evaluation of Carbon Nanoparticles in Layer-by-Layer Self-assembled Enzyme-based Biosensors","authors":"M. David, M. Florescu, M. Barsan, C. Brett","doi":"10.1016/J.PROTCY.2017.04.125","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.125","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"37 1","pages":"304-305"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84864759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycelial Extracts as Sensitive Element for Acoustoelectronic Gas Sensor","authors":"I. Kuznetsova, B. Zaitsev, A. Shikhabudinov, O. Tsivileva, A. Pankratov","doi":"10.1016/J.PROTCY.2017.04.113","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.113","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"96 1","pages":"265-267"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78615268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical classification and adulteration detection of cumin by using electronic sensing coupled to multivariate analysis","authors":"K. Tahri, C. Tiebe, N. E. Bari, T. Hübert, B. Bouchikhi","doi":"10.1016/J.PROTCY.2017.04.102","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.102","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"30 1","pages":"240-241"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76456183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced performance of electrochemical sensors based on nanosized NiO particles","authors":"M. Carbone, Alessia Nesticò, Noemi Bellucci, L. Micheli, G. Palleschi","doi":"10.1016/J.PROTCY.2017.04.117","DOIUrl":"https://doi.org/10.1016/J.PROTCY.2017.04.117","url":null,"abstract":"","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"30 1","pages":"277-278"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86961894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Sensitive Escherichia coli Shear Horizontal Surface Acoustic Wave Biosensor with Silicon Dioxide Nanostructures","authors":"S.T. Ten ,&nbsp;U. Hashim ,&nbsp;A.N. Nordin ,&nbsp;S.C.B. Gopinath ,&nbsp;W.W. Liu ,&nbsp;K.L. Foo ,&nbsp;S.T. Sam ,&nbsp;S.F.A. Rahman ,&nbsp;C.H. Voon","doi":"10.1016/j.protcy.2017.04.014","DOIUrl":"10.1016/j.protcy.2017.04.014","url":null,"abstract":"<div><p>In this study, a shear horizontal surface acoustic wave (SHSAW) was used for <em>Escherichia coli</em> (<em>E. coli</em>) detection. <em>E. coli</em> O157:H7 serotype, a dangerous strain among 225 <em>E. coli</em> unique serotypes was chosen as test sample. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1 cfu <em>E. coli</em> O157:H7 in 25 g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64⁰ YX LiNbO₃ substrate. Five different interdigital transducers (IDT) parameters (Fig. 1(a)-1(e)) SHSAW were fabricated to compare the mass loading sensitivity before advancing to the bio sensing application: four of them were 32 μm pitch size (with the average of synchronous frequency, f₀ = 144.303 MHz) and one was 12 μm pitch size (with f₀ = 384.948 MHz). All of the four 32 μm pitch sizes were with different combination of delay line length (3.904 mm and 7.296 mm) and aperture size (1.376 mm and 2.464 mm). As for the 12 μm pitch size device, it was with 2.1 mm delay line length and 0.72 mm aperture size. Eventually, 12 μm pitch size device was selected for oligonucleotides detection and its mass loading sensitivity was 1558.04 MHz/ (mg/mm²), 4.8-fold higher than the most sensitivity one in 32 μm pitch size).</p><p>Its sensitivity was enhanced by depositing 130 nm thin layer of SiO2 nanostructures with particle size less than 70 nm (Fig. 1(f)-1(g)). The nanostructures act both as a waveguide as well as the surface physical modification of the sensor prior to biomolecule immobilization. For this work, a specific DNA sequence from <em>E. coli</em> O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and (APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the oligonucleotide target and attained the sensitivity of 0.6439 nM/ 0.1 kHz and detection limit was down to 1.8 femtomolar (1.8 x 10^-15 M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 29-30"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75785371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stilbene Switch Activated by Click Chemistry","authors":"Yubin Zhou ,&nbsp;Yuanyuan Wu ,&nbsp;Oleksandr Pokholenko ,&nbsp;Vladislav Papper ,&nbsp;Robert S. Marks ,&nbsp;Terry W.J. Steele","doi":"10.1016/j.protcy.2017.04.006","DOIUrl":"10.1016/j.protcy.2017.04.006","url":null,"abstract":"<div><p>Stilbenes have not been widely regarded as fluorophore labels due to their difficulty in conjugation. Quenched fluorescence of maleimide functionalized stilbene is found to be restored after thiol binding, enabling separation-free fluorophore labelling.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 10-11"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85683026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity of Fullerenols. Bioluminescent Monitoring in vitro","authors":"A.S. Sachkova ,&nbsp;E.S. Kovel ,&nbsp;A.A. Vorobeva ,&nbsp;N.S. Kudryasheva","doi":"10.1016/j.protcy.2017.04.097","DOIUrl":"10.1016/j.protcy.2017.04.097","url":null,"abstract":"<div><p>Bioluminescence of isolated enzymes is a perspective phenomenon for biosensors development due to simplicity of registration of a physiological parameter - light intensity. Enzyme-based bioluminescent assay is widely used to evaluate a decrease in biochemical toxicities. Also the enzyme-based assay is used for the direct biochemical monitoring of oxidative toxicity. This work considers antioxidant properties of fullerenols, water-soluble polyhydroxylated derivatives of fullerenes and perspective pharmaceutical agents, in solutions of model inorganic and organic toxicants of oxidative type – K₃[Fe(CN)₆] and 1,4-benzoquinone. Two fullerenol preparations were used: C₆₀O_2-4(OH)_20-24 and mixture of two types of fullerenols C₆₀O_2-4(OH)_20-24+C₇₀O_2-4(OH)_20-24. The enzyme-based assays showed the peculiarities of the detoxification processes: ultralow concentrations of fullerenols were active (<em>ca</em> 10^-17-10^-5g/L); no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effects of highly diluted fullerenol solutions were attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 230-231"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79260839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards a Self-powered Biosensors for Environmental Applications in Remote, Off-grid Areas","authors":"Grzegorz Pasternak ,&nbsp;John Greenman ,&nbsp;Ioannis Ieropoulos","doi":"10.1016/j.protcy.2017.04.005","DOIUrl":"10.1016/j.protcy.2017.04.005","url":null,"abstract":"<div><p>One important factor for developing biosensors is taking the source of electrical energy into account. The source of electricity is needed whenever we consider point-of-care diagnostics, in-vivo tests or in particular – environmental applications. The need of supplying energy to biosensor may be an important obstacle for its everyday use, particularly in developing countries. Here, we present the concept of biosensor able to generate power by bioelectrochemical oxidation of the analyte and thus – able to autonomously maintain its operation.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 8-9"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89252333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Sensitive Electrospun Multiwalled Carbon Nanotubes Embedded Zinc Oxide Nanowire Based Interface for Label Free Biosensing","authors":"K. Brince Paul,&nbsp;Siva Rama Krishna Vanjari,&nbsp;Shiv Govind Singh","doi":"10.1016/j.protcy.2017.04.091","DOIUrl":"10.1016/j.protcy.2017.04.091","url":null,"abstract":"<div><p>We demonstrate synthesis of Multiwalled carbon nanotubes (MWCNTs) embedded highly oriented Zinc Oxide (ZnO) nanowires targeted towards development of ultrasensitive electrochemical nanobiosensors using electrospinning method. The synthesized composite nanowires combines advantages of ZnO such as biocompatibility, electrostatic affinity towards biomolecules with the excellent conductivity and surface functionalization capabilities of MWCNTs. Calcination temperature is optimized so as to ensure MWCNTs are present in their original form and at the same time highly crystalline ZnO is obtained. The key advantage of this process is that there is no separate functionalization process is required to create functional groups on MWCNTs. Furthermore, the electrochemical activity of MWCNTs embedded ZnO nanowires is much higher as compared to pure ZnO nanowires. We have demonstrated the performance of electrochemical nanobiosensor using Biotin-streptavidin interaction as model system. The sensor exhibits excellent sensitivity in the range 10 μgmL^-1 - 0.5 fgmL^-1 of streptavidin with 0.5fgmL^-1 limit of detection.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 217-218"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75464058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}