Highly-sensitive Label-free Differential Pulse Voltammetric Immunosensor for Diagnosis of Infectious Diseases Based on Electrospun Copper Doped ZnO Nanofiber Biosensing Platform

K. Brince Paul , Sanni Kumar , Suryasnata Tripaty , Vikrant Singh , Siva Rama Krishna Vanjari , Shiv Govind Singh
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引用次数: 1

Abstract

Rapid detection of infectious diseases has generated significant interest in recent years. The time consuming and costly conventional diagnostics methods substantiate the need to develop a cost-effective rapid infectious disease detection platform to address the persistently threatening health issues in developing countries. The recent advancements in nanotechnology and biosensing have manifested the potential to deliver an effective point-of-care diagnostics platform. In this work, the synthesis and fabrication of an ultrasensitive Copper doped Zinc oxide nanofiber based biosensing platform is reported. Copper doped Zinc oxide nanofibers are synthesized by simple electrospinning technique with fiber diameter of 100-200 nm. The structural and morphological characteristics of the nanofibres are studied using X-ray diffraction and field emission scanning electron microscopy. The label free detection of HRP2 protein with the Copper doped Zinc Oxide nanofiber has been investigated by Differential pulse voltammetric technique. Mercaptopropionic acid treatment of Copper doped Zinc oxide nanofiber generates carboxylic acid groups, which facilitate the covalent conjugation of Anti-HRP2. To the best of our knowledge, the fabricated immunosensor displays better sensitivity than the best malaria sensor reported in the literature based on different nanomaterials and different detection mechanism. The proposed platform exhibits very low limit of detection of 10 attogram per ml for the targeted HRP2 protein in a wide detection test range (ag/ml -μg/ml). The novel biosensor platform demonstrates good stability and selectivity which can be implemented for point-of-care diagnosis of biomarkers related to other infectious diseases.

基于电纺铜掺杂ZnO纳米纤维生物传感平台的高灵敏度无标记差分脉冲伏安免疫传感器
近年来,对传染病的快速检测引起了极大的兴趣。由于传统诊断方法既耗时又昂贵,因此有必要开发一种具有成本效益的快速传染病检测平台,以解决发展中国家持续存在的威胁健康的问题。纳米技术和生物传感的最新进展显示出提供有效的即时诊断平台的潜力。本文报道了一种基于铜掺杂氧化锌纳米纤维的超灵敏生物传感平台的合成和制备。采用简单静电纺丝技术合成了铜掺杂氧化锌纳米纤维,纤维直径为100 ~ 200 nm。利用x射线衍射和场发射扫描电镜研究了纳米纤维的结构和形态特征。采用差分脉冲伏安法研究了铜掺杂氧化锌纳米纤维对HRP2蛋白的无标记检测。巯基丙酸处理后的铜掺杂氧化锌纳米纤维生成羧酸基团,有利于Anti-HRP2的共价偶联。据我们所知,所制备的免疫传感器比文献中基于不同纳米材料和不同检测机制的最佳疟疾传感器具有更好的灵敏度。该平台在较宽的检测范围(ag/ml -μg/ml)内,对目标HRP2蛋白的检测限极低,为10阿图克/ml。该新型生物传感器平台具有良好的稳定性和选择性,可用于其他传染病相关生物标志物的即时诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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