Pharmacological Research - Natural Products最新文献

{"title":"Diverse bioactivity of traditional herb Achyranthes aspera in ethnomedicine","authors":"Yuvaraj Dinakarkumar ,&nbsp;Gnanasekaran Ramakrishnan ,&nbsp;Ponmozhi Marimuthu ,&nbsp;Santhan Velayudham Priya ,&nbsp;Sharmila Kumar ,&nbsp;Vaagdevi Mukkavilli","doi":"10.1016/j.prenap.2025.100268","DOIUrl":"10.1016/j.prenap.2025.100268","url":null,"abstract":"<div><div>A popular ethnomedical herb, <em>Achyranthes aspera</em> has a long history in traditional medicine because of its many medicinal uses. Tannins, alkaloids, flavonoids, and saponins are among the many bioactive substances that have been found through phytochemical studies and contribute to its therapeutic qualities. This herb has been thoroughly investigated in contemporary pharmacology, revealing its anti-inflammatory, antimicrobial, and cardiovascular-modulating properties despite having strong roots in traditional treatments. It is important for treating oxidative stress-related conditions like degenerative and chronic inflammatory diseases because of its strong antioxidant activity. According to scientific research, the plant's anti-inflammatory qualities effectively lower inflammation by modifying pro-inflammatory cytokines. It has the potential to treat diseases naturally because of its antibacterial action, which has been shown against a variety of pathogens. Its pharmacological relevance is further expanded by its cardiovascular-modulating activities, which include antihypertensive and cardioprotective properties. Furthermore, <em>Achyranthes aspera</em>'s antioxidant qualities are essential in the fight against oxidative stress, which is connected to a number of chronic illnesses, such as cancer, diabetes, and neurological diseases. This review highlights the plant's potential to aid in the creation of innovative medicinal substances and as sources of novel drug development since it combines traditional medical practices with contemporary pharmacological understanding.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100268"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NMR characterization of stigmasterol in Emilia coccinea (Sims) G. Don leaves dichloromethane fraction","authors":"Viola A. Nwachukwu Nicholas-Okpara ,&nbsp;Stanley Chidi Udedi ,&nbsp;Francis Chukwuemeka Ezeonu ,&nbsp;Francis Oluwole Shode ,&nbsp;Gloria Nwakaegho Elemo ,&nbsp;Maryam Olanshile Adegboyega","doi":"10.1016/j.prenap.2025.100274","DOIUrl":"10.1016/j.prenap.2025.100274","url":null,"abstract":"<div><div>Natural product research holds great potential for the discovery of new drug leads. It has been established that natural plant products are used to cure and manage various human diseases. The leaves of <em>Emilia coccinea</em> (Sims) G. Don (genus Emilia) have long been used in African Traditional Medicine for their anti-cholesterolemic, cardiovascular, and wound-healing benefits. Consistent with existing literature, this study confirms the presence of stigmasterol as a major component in <em>E. coccinea</em> leaves through ¹D NMR (1H NMR and 13C NMR) and FT-IR analysis of its dichloromethane extract. Previous research has reported stigmasterol as a potent hypolipidemic and anti-obesity agent. Based on the comparison of its physical and spectroscopic features with existing literature, our work describes the method of isolation and NMR structure elucidation of stigmasterol obtained from <em>E. coccinea</em> leaves. While stigmasterol has previously been isolated from <em>E. coccinea</em> leaves using hexane and ethyl acetate, our research validates its isolation using dichloromethane as an alternative method. This ensures reproducibility and offers methodological flexibility for future research. Finally, we affirm that <em>Emilia coccinea</em> can serve as a new source of stigmasterol, a potent therapeutic agent, and could be a useful lead in the research and development of nutraceuticals for various diseases.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100274"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the therapeutic potential of selected natural products in combating osteoarthritis using experimental model – A review","authors":"Dayo Rotimi Omotoso ,&nbsp;Olaoluwa Aderinto","doi":"10.1016/j.prenap.2025.100276","DOIUrl":"10.1016/j.prenap.2025.100276","url":null,"abstract":"<div><div>Osteoarthritis is a chronic, degenerative joint disease condition that mainly affects weight-bearing joints of the body. The pathogenesis of osteoarthritis is characterized with synovial inflammation, progressive degradation of articular cartilage, subchondral bone changes, and joint dysfunction. There remains the persistent need for the development of effective therapeutic intervention that would offer improved outcomes compared to the existing therapeutic options. This review explores the current measure to harness the therapeutic potential of natural products, including berberine, apigenin, chrysin, baicalein and curcumin, in combating osteoarthritis, with a focus on the mechanisms of their anti-osteoarthritis effects. A review of relevant literature was conducted across scientific databases including PubMed, Web of Science, Scopus, and Google Scholar, examining studies on the anti-osteoarthritis effects of selected natural products. All the natural products under review exhibited anti-osteoarthritis based on their antioxidant, anti-inflammatory and anti-apoptotic activities which were achieved through their modulatory effect on oxidative stress, inflammatory, apoptotic mediators that drives the onset and progression of osteoarthritis. The anti-osteoarthritis properties of the natural products were achieved through modulation of signaling factors or pathways including IL-1β, IL-6, TNF-α, MMP-13, Caspase-3, HMGB-1, ADAMTS, MAPK, TlR4/NF-kB, hedgehog, Wnt/β-cathenin, IL-1 β, ERK1/2, Wnt/β-cathenin, AMPK/PINK1/Parkin, PERK/TXNIP/NLRP3, RAGE/PI3k/AKT.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100276"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity and structure elucidation of Pterosterone, a naturally occurring phytoecdysteroid isolated from the leaves of Vitex madiensis Oliv. (Lamiaceae)","authors":"Emmanuel Moke Lengbiye ,&nbsp;Koto-Te-Nyiwa Ngbolua ,&nbsp;Angélique Nicolas Messi ,&nbsp;Lin Marcellin Messi ,&nbsp;Beltus Fofack ,&nbsp;Auguste Abouem A. Zintchem ,&nbsp;Gwladys Sime Djikam ,&nbsp;Jean Faustin Atangana ,&nbsp;Damien Sha-Tshibey Tshibangu ,&nbsp;Cyprien Mbundu Lukukula ,&nbsp;Joséphine Ngo Mbing ,&nbsp;Dieudonné Emmanuel Pegnyemb ,&nbsp;Pius Tshimankinda Mpiana","doi":"10.1016/j.prenap.2025.100264","DOIUrl":"10.1016/j.prenap.2025.100264","url":null,"abstract":"<div><h3>Background</h3><div>Traditional uses and preliminary studies suggested that extracts from the leaves of <em>Vitex madiensis</em> might contain bioactive compounds with medicinal value.</div></div><div><h3>Purpose</h3><div>This study aimed to isolate and identify bioactive compounds from the leaves of <em>V. madiensis</em> and evaluate their antimicrobial activities. Additionally, the study sought to elucidate the structure of the isolated compound using spectroscopic methods and assess its stability and interactions with enzyme receptors through in silico studies.</div></div><div><h3>Study design</h3><div>Compounds were isolated from the ethyl acetate extract of <em>V. madiensis</em> leaves and evaluated for antibacterial and antifungal activities. The isolated compound underwent <em>in silico</em> studies to determine its stability, sensitivity, and interaction with enzyme receptors.</div></div><div><h3>Methods</h3><div>Leaves of <em>V. madiensis</em> were extracted with ethyl acetate, followed by chromatographic techniques to isolate the target compound. Antibacterial and antifungal properties were tested, determining minimum inhibitory concentration (MIC) values against bacterial and fungal strains. Stability, kinetic sensitivity, and interactions with β-lactamase and Sterol-14-alpha demethylase were assessed using computational methods. Structural elucidation was achieved using NMR, MS, IR, and literature comparisons.</div></div><div><h3>Results</h3><div>The isolated compound exhibited antibacterial (MIC=1000–15.6 µg/mL) and antifungal (MIC=1000 µg/mL) activities. In silico studies showed it is stable, has high kinetic sensitivity, and affinity with enzyme receptors (β-lactamase, ΔG= −8.16 kcal/mol; Sterol-14-alpha demethylase, ΔG= −8.56 kcal/mol). The compound was non-toxic and non-mutagenic.</div></div><div><h3>Conclusion</h3><div>The study isolated Pterosterone from <em>V. madiensis</em> leaves, demonstrating significant antibacterial and antifungal activities. Its non-toxic and non-mutagenic profile suggests potential as a natural antimicrobial agent. This is the first report of pterosterone from <em>Vitex madiensis</em>.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100264"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the therapeutic potential of Apium graveolens linn. and Persea americana mill. seed extract in modulating cellular targets for the treatment of diabetic retinopathy","authors":"Kamalesh D.R.,&nbsp;Geetha K.M.","doi":"10.1016/j.prenap.2025.100266","DOIUrl":"10.1016/j.prenap.2025.100266","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Purpose&lt;/h3&gt;&lt;div&gt;&lt;em&gt;Apium graveolens (AG) and Persea americana (PA)&lt;/em&gt; are potential herbal candidates traditionally being used for their therapeutic activities since ages. They are useful in managing diabetes and its associated complications. The study intends to scientifically evaluate the aqueous extracts of AG, and PA, for the management of diabetic retinopathy. Additionally, the study aimed to identify and analyze the phytochemical constituents of these extracts using gas chromatography-mass spectrometry (GC-MS) to understand their potential contribution to anti-diabetic and retinal protective effects.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Diabetes was induced by streptozotocin in Male adult Sprague-Dawley (SD) rats. The study was conducted later by the treatment of aqueous extracts of AG and PA for 16 weeks. Evaluation of the glycemic parameters, alkaline phosphatase levels, BAX levels, VEGF levels, protein levels, oxidative stress parameters, retinal vessel diameter and funduscopic studies were carried out. In addition, GC–MS analysis was performed using a Shimadzu GCMS system to identify the bioactive compounds present in the aqueous extracts.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The extracts alone showed moderate reduction in glycemic parameters, whereas their combination with insulin and metformin demonstrated remarkable effects. PA (250 mg/kg) + insulin reduced glucose levels by 83.7 %, nearing normal values. Protein levels in the aqueous humor and lens significantly improved, with AG (500 mg/kg) showing a 222.7 % increase. ALP levels, which were elevated in diabetic rats, decreased significantly with PA (500 mg/kg) (61 % reduction). VEGF levels, associated with retinal damage, were significantly reduced by PA (250 mg/kg) + metformin (62.6 %, &lt;em&gt;P&lt;/em&gt; &lt; 0.001). IL-1β, an inflammatory marker, was notably lowered by AG (500 mg/kg) (79.4 %) and PA (500 mg/kg) (63.3 %). Catalase activity increased significantly with AG (500 mg/kg) (103.6 %, &lt;em&gt;P&lt;/em&gt; &lt; 0.001), while ascorbic acid levels, depleted in diabetic conditions, were restored by AG (500 mg/kg) and AG + metformin, both exceeding a 100 % improvement (&lt;em&gt;P&lt;/em&gt; &lt; 0.001). Histopathological analysis showed improved retinal integrity and reduced cellular apoptosis. Vessel diameter measurements confirmed significant vascular restoration, supported by funduscopic analysis showing enhanced retinal preservation and reduced pathological changes. The AG extract was found to contain 17 components including 2,3-Butanediol (18.29 %), Tiglic acid (7.61 %), L-Lactic acid (4.44 %), and n-Hexadecanoic acid (2.83 %), whereas PA extract contained 15 components, with 2,3-Butanediol (13.05 %) being predominant.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;This study provides evidence that aqueous extracts of AG and PA possess therapeutic potential for managing diabetic retinopathy. While the extracts individually showed moderate effects in improving glycemic control and associated bio","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100266"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiglioma activity from Aloysia virgata (Ruíz & Pavón) Juss","authors":"Manoela Fernanda Schuster ,&nbsp;Gênifer Erminda Schreiner ,&nbsp;Nessana Dartora ,&nbsp;Lauren Lúcia Zamin","doi":"10.1016/j.prenap.2025.100267","DOIUrl":"10.1016/j.prenap.2025.100267","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Aloysia virgata</em> is a plant native from southern Brazil, belonging to the Verbenaceae family and popularly known as “lixeira” due to its rough leaves. Medicinally, this plant is used for the treatment of diseases which affect the digestive system, and some studies regarding its neuroactive activity have been reported.</div></div><div><h3>Aim of the study</h3><div>The aim of this study was to structurally characterize secondary metabolites found in the leaves of <em>A. virgata</em>, a medicinal plant. Additionally, we evaluated the antitumor potential of extracts and fractions obtained from the leaves of the plant against glioma cell lines.</div></div><div><h3>Materials and methods</h3><div>Dried and crushe leaves were subjected to aqueous (AC) and ethanolic extraction (EC). A liquid-liquid partitioning with different solvents in increasing order of polarity was performed to reduce the complexity of the extracts and facilitate their analysis. Thus, both extracts were solubilized in water and the following solvents were added in increasing order of polarity: chloroform, ethyl acetate, and butanol for EC, and ethyl acetate and butanol for AC. The partitioning process resulted in 4 fractions for EC, named ECH, EEA, EB, and EA and 3 fractions for AC, named AEA, AB, and AA. After obtaining the extracts and fractions, they were tested to assess their effect on cell viability. Cells from human glioma cell line U251 and rat cell line C6 were treated with all the extracts and fractions obtained at concentrations of 1, 5, and 10 mg/mL for 24, 48, and 72 hours, followed by the MTT assay. To simulate the treatment cycle used in the clinic, which consists of 5 days of treatment followed by 23 days without treatment, U251 cells were plated and exposed to treatments with AEA, ECH, and EEA for 5 days. After this period, the cells were maintained for additional 23 days without treatment.</div></div><div><h3>Results</h3><div>After characterization analyses, the presence of 28 major compounds were identified for both extracts and their respective fractions, of which only 5 had been previously described for this plant, with the majority belonging to the class of flavonoids. Cell viability tests revealed that treatments AEA, ECH, and EEA exhibited the highest rates of decrease in glioma cell viability and population doubling.</div></div><div><h3>Conclusions</h3><div>This study characterized metabolites present in the plant <em>A. virgata.</em> It was demonstrated for the first time that this extract exhibited cytotoxic effects on glioma cell lines, indicating potential to be further explored through additional studies for future application as a treatment for tumors.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100267"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the secrets of a miracle tree (Moringa oleifera) in Tanzania: Integrating traditional therapy with modern science","authors":"Hamisi Yunus Nchimbi","doi":"10.1016/j.prenap.2025.100265","DOIUrl":"10.1016/j.prenap.2025.100265","url":null,"abstract":"<div><div>A socio-ethnobotanical survey in Ngh’ongh’onha village, Dodoma, investigated demographic influences on Moringa oleifera knowledge and use. Older individuals (over 46 years, 65.6 %) had substantial knowledge, whereas younger people (18–30 years, 13.6 %) showed minimal acquaintance, primarily acquired through family influence. Women (58.18 %) were mainly responsible for herbal medicine preparation, while men (38 %) focused on cultivation and processing. Knowledge retention was greater among uneducated people (46.36 %) relying on oral traditions, while those with formal education (3.63 %) preferred modern treatments. Experienced individuals (over 15 years, 48.2 %) acted as community advisors, while those with less experience (0–5 years, 10 %) had little involvement. M. oleifera seed oil (15 %) was used to treat fungal and joint infections; seeds/leaves (13 %) to boost immunity; boiled leaves (11 %) for colds and flu; and stem bark (10 %) for joint pain. Other uses included cancer (7 %), malaria/anaemia (6 %), diabetes (5 %), and breastfeeding support (flowers). These findings highlight M. oleifera's broad pharmacological actions and antimicrobial potential. Further research identified bioactive compounds and evaluated the antibacterial properties of M. oleifera leaf extracts to integrate traditional knowledge with scientific validation. Samples were collected from forested areas in Ngh’ongh’onha and analysed at the University of Dodoma. Phytochemical analysis via Agilent GC-MS revealed 53 bioactive compounds. Major constituents included Lanosterol (20.57 %), Germanicol (15.1 %), Phytol (10.65 %), and β-Amyrone (9.7 %). Extracts exhibited antibacterial, antifungal, antioxidant, anti-inflammatory, and anticancer properties, with inhibition zones of 16.6–19.8 mm against six microbial strains. This study confirms M. oleifera's ethnobotanical, antimicrobial, and therapeutic potential for pharmaceutical applications.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100265"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aqueous extract of Cucurbita pepo L. Fruits exhibits nephroprotective activity in a mouse model of gentamicin-induced nephrotoxicity using In Vivo and In Silico approaches","authors":"Da Filkpièrè Léonard ,&nbsp;Tindano Basile ,&nbsp;Aboubakar Oumarou Bibi Farouck ,&nbsp;Owona Pascal Emmanuel ,&nbsp;Soudré Albert ,&nbsp;Bayala Balé ,&nbsp;Belemtougri Gourounga Raymond ,&nbsp;Dimo Théophile","doi":"10.1016/j.prenap.2025.100261","DOIUrl":"10.1016/j.prenap.2025.100261","url":null,"abstract":"<div><h3>Background</h3><div>The leaves and the fruits of <em>Cucurbita pepo</em> L. (Cucurbitaceae) are traditionally used by the Lobi communities of Burkina Faso, Côte d′Ivoire and Ghana as a food and diuretic stimulant for children.</div></div><div><h3>Aim</h3><div>To evaluate the nephroprotective effect of the aqueous extract of <em>Cucurbita pepo</em> L. fruits against gentamicin-induced kidney damage.</div></div><div><h3>Methods</h3><div>Nephrotoxicity was induced in Wistar rats by intraperitoneal injection of gentamicin (80 mg/kg). The effects of aqueous extract of <em>Cucurbita pepo</em> fruits administered orally for 8 consecutive days at doses of 100 and 200 mg/kg were assessed on renal and oxidative markers. HPLC-MS separation was performed on a Zorbax Eclipse XDB-C18 reversed-phase column maintained at 40°C with water/acetonitrile acidified with 0.1 % methanoic acid as mobile phase Results:</div><div>Extract at doses of 100 and 200 mg/kg significantly reduced (p &lt; 0.05) gentamicin-induced elevations in serum creatinine, serum uric acid and renal malondialdehyde. It significantly (p &lt; 0.05) increased urine volume, glomerular filtration rate and decreased concentrations of urinary creatinine, urinary uric acid, serum protein and renal glutathione due to gentamicin. Extract at 100 and 200 mg/kg also significantly (p &lt; 0.05) increased antioxidant enzyme activity (catalase, superoxide dismutase) in the kidney. Histology of kidney sections from animals treated with extract and vitamin C showed a reduction in pathological features compared with gentamicin-treated animals. LC-MS analysis show that, <em>Cucurbita pepo</em> fruits possesse melezitose, quinique acid, rinderine, scopletin, carboxynorspermidine, androstane-3,17-dione, kaur-9 (11) acid, 16-dienoique, and alpha bisabolol who have shown the capacity to bind to Factor Nuclear Kappa Beta (NF-κβ) and Necrosis Tumoral Factor alpha (TNF-α) proteins.</div></div><div><h3>Conclusion</h3><div>The results show that the nephroprotective potential of aqueous extract of <em>Cucurbita pepo</em> L. fruits due to the probable activities of its phytochemical components can be used against the gentamicin induced toxicity in kidney.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100261"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A leafy vegetable, Marsilea minuta L.: Review of ethnomedicine, phytochemistry, and pharmacological properties","authors":"Varsha Yadav ,&nbsp;Sinchan Das ,&nbsp;Souvik Mukherjee ,&nbsp;Altamash Khan ,&nbsp;Vivekananda Mandal ,&nbsp;Swaha Satpathy ,&nbsp;Manoj Goyal ,&nbsp;Arjun Patra","doi":"10.1016/j.prenap.2025.100263","DOIUrl":"10.1016/j.prenap.2025.100263","url":null,"abstract":"<div><h3>Introduction</h3><div>Medicinal plants have been a source of therapeutic agents for the treatment and management of various disease conditions in humans and animals. For research on natural products or medicinal plants, the most significant step is proper selection of a plant species. Furthermore, to carry out research in a specific direction, an extensive literature survey and finding the gap is unavoidable. A leafy vegetable, <em>Marsilea minuta</em> L. has been traditionally used for treating different diseases.</div></div><div><h3>Methodology</h3><div>Literature available in Scopus database was retrieved by using the keyword: <em>Marsilea minuta</em> and all documents were downloaded, screened and considered the traditional uses, phytochemistry and pharmacological activities. In this review, we have compiled the traditional uses, phytochemistry and pharmacological activities of <em>M. minuta</em>. We have also analyzed all the documents and presented some bibliometric research scenario on this plant species.</div></div><div><h3>Results</h3><div>Traditionally the plant is used in diseases of nervous, digestive, respiratory and cardiovascular system. It is also employed in diabetes, migraine, body pain, insomnia and cough. The major chemical compounds identified in the plant include flavonoids such as quercetin-3-O-glucoside, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, chalcone-3-O-glucoside, quercetin-3-rutinoside, and naringenin-7-O-glucoside; and phenolic acids like gallic acid, caffeic acid, 4-hydroxy-3-methoxy benzoic acid, p-coumaric acid, m-coumaric acid, chromatoropic acid, vanillic acid, syringic acid, ferulic acid, and chlorogenic acid. The plant showed antidepressant, anxiolytic, hepatoprotective, antitussive, antioxidant, antimicrobial, anti-aggressive, antipyretic and analgesic activities. The leafy vegetable contains different bioactive compounds, which might be responsible for biological activities. The plant species could be developed as a nutraceutical.</div></div><div><h3>Conclusion</h3><div>This collection and collation of information confirms the potential of <em>M. minuta</em> as a drug candidate and would be helpful for researchers to further explore this plant species.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100263"},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the multifaceted wound healing potential of Grewia asiatica leaf extracts in incision, excision and cotton pellet models","authors":"Abhay Kumar Upadhyay,&nbsp;Shivangi Kumari,&nbsp;Tuhin Mukherjee,&nbsp;Nikita Nayak,&nbsp;Ravi Pratap Singh,&nbsp;Payel Mal,&nbsp;Satyajit Mohanty,&nbsp;Ashok Pattnaik","doi":"10.1016/j.prenap.2025.100258","DOIUrl":"10.1016/j.prenap.2025.100258","url":null,"abstract":"<div><h3>Background</h3><div>Wound healing therapy has advanced significantly as there is still no promising therapy to handle the intricacy of different wound types. This calls for the upbringing of agents with proper formulation for detailed wound healing.</div></div><div><h3>Objectives</h3><div>The aim of this study was to identify the appropriate solvent extract of <em>Grewia asiatica</em> (Linn) through a series of phytochemical analyses. Subsequently, the selected extract was incorporated into a suitable topical formulation (gel) and evaluated for its effectiveness as a wound-healing agent. The evaluation involved <em>in-vitro</em> and <em>in-vivo</em> modes using responsible enzymes and animal models (Swiss albino mice) to assess physical, biochemical, and histological parameters.</div></div><div><h3>Materials and methods</h3><div>In order to investigate the wound healing efficacy, various analyses were conducted including DPPH, ABTS, nitric oxide and hydrogen peroxide scavenging activity assays, estimation of total phenolic and flavonoid content, analgesic activity, and histopathology studies using excision, incision, and cotton pellet wound models. The Ethyl acetate extract was found to have the highest concentration of desirable phytochemicals and was therefore incorporated in <em>in-vivo</em> experiments. The percentage of wound contraction in mice and tensile strength were assessed in various wound models.</div></div><div><h3>Results</h3><div>The results of the study showed that the ethyl acetate fraction was found to be effective in suppressing DPPH, nitric oxide, and hydrogen peroxide when compared to ascorbic acid as standard. Additionally, when compared to the methanol and control groups, the group treated with ethyl acetate demonstrated the highest percentage of wound contraction and improved tensile strength. Morphological and histopathological examinations of the regenerated skin indicated a noticeable improvement in healing. These findings suggest that the formulated gel has significant wound-healing properties.</div></div><div><h3>Conclusion</h3><div>The plant was selected primarily based on its traditional use in folklore medicine. The traditional use was scientifically validated through a series of successful in vitro and in vivo experiments, which established the plant's potent anti-oxidant and skin regeneration capacity. These findings strongly suggest that the plant has significant wound-healing activity.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100258"},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}