Pharmacological Research - Natural Products最新文献

{"title":"Unlocking the mechanistic potential of clinically proven natural products against Arthritis","authors":"Md. Abdul Karim Jiyad ,&nbsp;Md. Sakibul Alam ,&nbsp;Priya Devi ,&nbsp;Mohammad Shakawat ,&nbsp;Nabila Ishrat ,&nbsp;Sabrina Alam Mrittika ,&nbsp;Khurshida Jahan Suma ,&nbsp;Md. Kausar Uddin Ahmed ,&nbsp;Shahjalal Chowdhury ,&nbsp;Md. Saqline Mostaq ,&nbsp;Nor Mohammad ,&nbsp;Farjana Afrin Tanjum ,&nbsp;Farhana Sultana ,&nbsp;Mohammad Nurul Amin","doi":"10.1016/j.prenap.2025.100183","DOIUrl":"10.1016/j.prenap.2025.100183","url":null,"abstract":"<div><h3>Background</h3><div>Hundreds of millions of people worldwide suffer from joint illnesses. In terms of joint diseases, arthritis is the most common. Almost 100 different rheumatic diseases that impact muscles, joints, cartilage, ligaments, and tendons are collectively referred to as arthritis, which literally means \"joint inflammation”.</div></div><div><h3>Objectives</h3><div>The mechanisms, safety, and efficacy of natural products that have been clinically studied in the treatment of arthritis including rheumatoid arthritis and knee osteoarthritis, are comprehensively discussed in this article.</div></div><div><h3>Methods</h3><div>PubMed, Science Direct, Elsevier, and Google Scholar databases were searched for suitable articles about arthritis using the keywords- “natural products used in arthritis”, “clinically proven natural products against arthritis”, “rheumatoid arthritis”, “knee osteoarthritis” or keywords similar to those. This review has only included natural products whose anti-inflammatory and anti-arthritic properties have been clinically demonstrated.</div></div><div><h3>Result</h3><div>Clinically proven natural products that are reported to exhibit anti-arthritic potential include Burdock root, Avocado, Honey bee, Devil’s claw, <em>Boswellia serrate</em>, <em>Clausena anisata</em>, Rose hips, Feverfew, Evening primrose oil, Sesame seed, Eggshell membrane, Resveratrol, Ginger extract, Silymarin, Gamma-linoleic acid, Curcuminoid, magnesium, <em>Andrographis paniculata</em>, <em>Elaeagnus angustifolia</em> extract, Peganum oil. All those natural products seemed to work on some common pathways such as inhibition of nitric oxide production, COX-2 enzyme, interleukin-2 production, nuclear factor-κB, etc.</div></div><div><h3>Conclusion</h3><div>According to our review, the above natural products can effectively treat arthritis without causing any significant side effects. Anyone can consider the aforementioned products as dietary supplements for the management of arthritis.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100183"},"PeriodicalIF":0.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractionation, HRLC-MS assisted phytochemical profiling, quantification, and evaluation of the chronic wound healing potential of ethanolic extract of Ardisia solanacea (Poir.) Roxb.","authors":"Mohini Tawade ,&nbsp;Jyotsna Chopade ,&nbsp;Asha Thomas ,&nbsp;Devendra Shirode","doi":"10.1016/j.prenap.2025.100182","DOIUrl":"10.1016/j.prenap.2025.100182","url":null,"abstract":"<div><h3>Background</h3><div>Wound healing is a complex, multi-stage biological process involving coordinated events such as haemostasis, inflammation, proliferation, and remodelling. When these stages fail to proceed in a timely and orderly manner, chronic wounds can develop. Herbal medicines, contain a blend of phytoconstituents, can act on multiple phases of wound healing and, being naturally derived, may have fewer side effects. <em>Ardisia solanacea</em> (Poir.) Roxb., a member of the genus <em>Ardisia</em>, has been used in traditional medicine for its wide-ranging activities, including antioxidant, anti-inflammatory, antimicrobial, antibacterial, and antidiabetic, etc. The present study aims to analyze and quantify the phytochemicals in the ethanolic extract from <em>A. solanacea</em> using various chromatographic techniques, and to assess its <em>in vivo</em> chronic wound-healing potential.</div></div><div><h3>Methods</h3><div><em>In vitro</em> antioxidant potential of ethanolic extract was evaluated by metal chelating assay. The phytochemical profiling was conducted through chromatographic techniques such as flash chromatography, HRLC-MS, and HPTLC. The chronic wound healing potential of <em>A. solanacea</em> was evaluated using an excision wound model, where diabetes mellitus was induced in wistar albino rats using alloxan monohydrate (120 mg/kg).</div></div><div><h3>Results</h3><div>The ethanolic extract of <em>A. solanacea</em> showed an IC₅₀ value of 65.41 ± 0.80 µg/mL, compared to 61.09 ± 1.12 µg/mL for BHT (standard). Phytochemical profiling through flash chromatography isolated 48 fractions, with fraction no. 12 being further analyzed by HRLC-MS, which identified 26 secondary metabolites. Among these were newly discovered compounds, including sinensetin, along with several flavonoid, phenolic, and alkaloid derivatives. HPTLC was employed to quantify sinensetin in both the ethanolic extract and the emulgel formulation. Pharmacological evaluation in wistar albino rats revealed that the formulated <em>Ardisia solanacea</em> emulgel, 10 % w/w has significant chronic wound healing potential, achieving 93.04 % ± 0.32 wound contraction by the 21st day, and a significantly shortened epithelization period (p &lt; 0.05) compared to diabetic control group.</div></div><div><h3>Conclusion</h3><div>To the best of our knowledge, this is the first study to demonstrate the chronic wound healing potential of the ethanolic extract of <em>A. solanacea</em> through excision wound model. This study supports the traditional use of <em>A. solanacea</em> for treating wounds and provides the scientific evidence confirming its chronic wound healing potential.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100182"},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salidroside: Diverse synthetic pathways and recent advances in pharmacological effects across multiple systems","authors":"Min Zhao ,&nbsp;Liuxiang Feng ,&nbsp;Wenhua Li","doi":"10.1016/j.prenap.2025.100176","DOIUrl":"10.1016/j.prenap.2025.100176","url":null,"abstract":"<div><div>Salidroside stands as a prominent compound derived from <em>Rhodiola</em> plants, enjoying a long history of integration within traditional Chinese medicine formulations. <em>Rhodiola</em> species predominantly thrive in challenging environments, often at high altitudes. Although the scale of salidroside extraction has broadened, the natural occurrence of this compound remains notably scarce. Prior reviews predominantly explored salidroside's antioxidant, anti-inflammatory, and anti-hypoxia properties. The effects of the compound on cardiovascular, brain, central nervous system, respiratory system, kidney, skeletal muscle, gastrointestinal system and liver were described. We provide an updated overview of salidroside's biological characteristics, mechanisms of action from 2019 to present, and its toxicological profile. Furthermore, we explore variations in salidroside sourced from different genera and delve into its synthetic pathways. This comprehensive analysis aims to address supply and demand challenges, pave the way for new drug research and development, and offer valuable insights for the continued exploration and utilization of salidroside.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100176"},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of antiurolithiatic properties of methanolic extract of rhizome of CyperusTegetumRoxb in ethylene glycol-induced urolithiatic rats","authors":"Shaibal Chandra,&nbsp;Nirupam Mondal,&nbsp;Atanu Chatterjee,&nbsp;Debarghya Tapadar","doi":"10.1016/j.prenap.2025.100181","DOIUrl":"10.1016/j.prenap.2025.100181","url":null,"abstract":"<div><div>Urolithiasis is known as urinary stone or kidney stone. It is the supersaturation of urine with the offending salt and crystallization. Crystals retained in the kidney can become a nucleus and form a stone-like structure. Novel therapeutic interventions are required for the treatment of urolithiasis with lower side effects. <em>Cyperuspangorei</em> Rottb.(synonyms- <em>Cyperus Tegetum Roxb</em>),is a CYPERACEAE family plant that is rich in bioactive phytochemicals and has shown promising pharmacological properties. The main goal of this study is to investigate the protective effects against urolithiasis of <em>C.pangorei</em> methanolic extract using ethylene glycol-induced kidney stones. Different groups of albino Wister rats received either a standard drug (50 mg/kg) of allopurinol or <em>C.pangorei</em> methanolic extract along with ethylene glycol (1 % v/v) except group I and group II, for a specific period. Group I received only a vehicle and Group II received a vehicle and ethylene glycol. After the specific time of duration, biochemical analysis (calcium, urea, uric acid, creatinine, phosphate, GPT, GOT) &amp; MDA analysis indicate significant protective effects against urolithiasis in treated with <em>C.pangorei</em> methanolic extract as compared to the untreated group and this plant have an antioxidant effect. Biochemicals of calcium, urea, uric acid, creatinine, phosphate, GPT, and GOT were effectively reduced by plant extract in serum. Results of histopathology proved that this plant has an antiurolithiatic effect on ethylene glycol-induced urolithiasis in rats.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100181"},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the vasodilatory, tocolytic and spasmolytic effects of rutin on Wistar rats tissues","authors":"Andressa de Alencar Silva ,&nbsp;Luís Pereira-de-Morais ,&nbsp;Paulo Ricardo Batista ,&nbsp;Cícera Georgia Brito Milfont ,&nbsp;Isaac Moura Araújo ,&nbsp;Carla Mikevely de Sena Bastos ,&nbsp;Irwin Rose Alencar de Menezes ,&nbsp;Yedda Maria Lobo Soares de Matos ,&nbsp;Roseli Barbosa","doi":"10.1016/j.prenap.2025.100172","DOIUrl":"10.1016/j.prenap.2025.100172","url":null,"abstract":"<div><div>Rutin is a phenolic compound belonging to the class of citrus flavonoids. This compound can be found in abundantly in buckwheat and other widely consumed foods such as onions, red beans, apples, tomatoes, and others. Some studies attribute the following activities to rutin: inhibitor of endothelial dysfunction, antioxidant, and cardioprotective. However, its relaxing activity on smooth muscles of rat tissues remain poorly studied. Therefore, this study investigated the vasodilatory, tocolytic, and spasmolytic effects of rutin in aorta, uterus, and ileum of rats using different contractile agonists. The experiments were carried out using the organ bath technique, in which the organs were placed in glass chambers with 10 mL of Tyrode’s solution for 1 h (stabilization). After this period, contractions in the aortic ring, myometrial and intestinal preparations were evoked using KCl (60 mM) for the electromechanical contractile stimulus, or different agonists (PHE 0.1 μM, OT 10⁻² IU/mL and CCh 10⁻⁶ M) for the pharmacomechanical stimulus. Then, increasing and cumulative concentrations of rutin (0.03 – 7.0 mM) were administered in the experimental preparations. In this study, it was observed that rutin has a peculiar pharmacological potential depending on the organ and the contractile agents evaluated. Therefore, future perspectives include the evaluation of the myorelaxant effect of rutin from other contractile agents in the aorta, uterus, and ileum, as well as more in-depth studies of the mechanisms for a better understanding of the pharmacological action of rutin in these organs.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100172"},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel insight into the anti-diabetic and diuretic potentials of the Colocasia esculenta L. (Taro) vegetable flower extract accentuating its ethnobotanical importance","authors":"Mahathir Mohammad ,&nbsp;Md. Rabiul Hossain ,&nbsp;Md. Mahmudul Hasan ,&nbsp;Fahmida Tasnim Richi ,&nbsp;Abdul Motaleb Bhuiya ,&nbsp;Shantu Chowdhury ,&nbsp;Rajia Sultana ,&nbsp;Safaet Alam ,&nbsp;Rashadul Hossain","doi":"10.1016/j.prenap.2025.100177","DOIUrl":"10.1016/j.prenap.2025.100177","url":null,"abstract":"<div><div><em>Colocasia esculenta</em> L. (Family: Araceae), also known as taro, is a tropical plant indigenous to Southeast Asia and the Pacific Islands with diversified ethnobotanical importance. This plant has long been utilized for its medicinal value in managing diabetes and hypertension. This study aims to explore the anti-diabetic and diuretic potentials of methanolic extracts of <em>Colocasia esculenta</em> flowers (CEF-ME). The anti-diabetic activity was evaluated by using <em>in-vitro</em> alpha-amylase and alpha-glucosidase enzyme inhibition assays while the diuretic effect of CEF-ME were employing Wistar rats model by measuring urine volume and electrolyte excretion level (Na⁺, K⁺, Cl⁻), with furosemide acting as the reference drug. The acute oral toxicity also carried on to shed light on the safety profile. Compounds identified in this plant were further subjected to molecular docking and ADME/T studies to validate the wet lab findings and to establish its drug-likeness profile. In hypoglycemic assays, CEF-ME exhibited a strong inhibition of alpha-amylase and alpha-glucosidase, with IC₅₀ values of 466.92 μg/mL (R² = 0.9899) and 653.53 (R² = 0.9878) μg/mL, respectively comparable to standard acarbose. At a 750 mg/kg dose, CEF-ME showed a 95 % increase in urine excretion, comparable to furosemide, and promoted electrolyte balance with increased Na⁺ excretion. Molecular docking studies revealed Cyclohexane-12-diol as a potent bioactive compound in CEF-ME, exhibiting strong binding affinities with responsible targets. These findings propose that CEF-ME could be a potential natural therapeutic agent for dealing with diabetes and hypertension, offering a dual action of glycemic control and fluid-electrolyte regulation.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100177"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stilbenes in breast cancer treatment: Nanostilbenes an approach to improve therapeutic efficacy","authors":"Javed Ahamad ,&nbsp;Sana Aza Ismail ,&nbsp;Abeer Zuheyr Jalal ,&nbsp;Mohammad Shabib Akhtar ,&nbsp;Javed Ahmad","doi":"10.1016/j.prenap.2025.100173","DOIUrl":"10.1016/j.prenap.2025.100173","url":null,"abstract":"<div><div>Stilbenes are polyphenolic secondary metabolites abundantly present in the various plant families and having a general structural formula of C₆-C₂-C₆ with two phenyl rings. Major bioactive phytochemicals belonging to stilbenes such as resveratrol, pterostilbene, and piceatannol are extensively studied for their role in the prevention and treatment of different cancers including breast cancer. These stilbenes show their anticancer potential through autophagy by regulating different signaling pathways (such as the PI3K/AKT, RAS/RAF/ERK, PKCδ, and AMPK) and promoting apoptosis. The major limitations in the utilization of these phytochemicals as a therapeutic are their low aqueous solubility and extensive metabolism that leads to poor biopharmaceutical performance in the <em>in vivo</em> systems. To overcome these limitations, nanotechnology emerged as a frontline approach that involved the fabrication of nanoparticulate systems of polymeric/lipidic/inorganic origin to encapsulate the stilbenes category of bioactive phytochemicals as a therapeutic cargo for the prevention and treatment of breast cancers. Nnaostilbenes as a nanoparticulate system are well-designed with the capability for site-specific delivery of loaded bioactives and significantly improve the therapeutic efficacy of encapsulated bioactives. Nanostilbenes in the forms of micelles, liposomes, nanoemulsions, nanotubes, and nanoparticles have been found to increase biopharmaceutical performance by improving aqueous solubility, decrease rapid degradation/elimination, and enhance cellular uptake at the breast tumor site. The present review provides a comprehensive discussion on biogenesis, chemistry, and biopharmaceutical challenges of different stilbenes. Furthermore, nanostilbenes may provide better efficacy against breast cancer due to improved biopharmaceutical performance of loaded bioactives employing a nanotechnology approach.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100173"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico screening and metabolomic profiling identify Naltrindole, a unique isoquinoline from Lentinus squarrosulus (Mont.,1842) mushroom having potent anti-diabetic properties","authors":"Neha Majumder,&nbsp;Debarupa Hajra,&nbsp;Ishita Bhattacharya,&nbsp;Ribhu Ray,&nbsp;Santanu Paul","doi":"10.1016/j.prenap.2025.100175","DOIUrl":"10.1016/j.prenap.2025.100175","url":null,"abstract":"<div><div>The increasing mortality associated with diabetes mellitus mandates the development of safer alternatives to the currently prescribed drugs that often lead to various health complications. Traditional herbal medicines are safer alternative interventions for managing diabetes mellitus. This study was carried out to determine the anti-diabetic efficacy of <em>Lentinus squarrosulus</em> (Mont.,1842)<em>,</em> a medicinal mushroom belonging to the family Polyporaceae. The methanolic extract of <em>Lentinus squarrosulus</em> (Mont.,1842) showed potent antioxidant and anti-diabetic effectuality indicated by robust 2,2-dipheyl-1-picrylhydrazyl (DPPH) radical scavenging activity (EC₅₀ value of 235 ± 4.66 µg/mL) and effective inhibition of the two crucial enzymes associated with diabetes i.e., α-amylase (IC₅₀ value of 317.2 ± 2.50 µg/mL) and α-glucosidase (588.1 ± 8.33 µg/mL). This result encouraged us to carry out the metabolomic profiling of the <em>Lentinus squarrosulus</em> (Mont.,1842) lead extract by liquid chromatography-mass spectrometry (LC-MS), which identified 52 lead mycocompounds. Absorption distribution metabolism excretion toxicity (ADMET) screening affirmed the drug-likeness of only 11 compounds. Robust <em>in-silico</em> screening of the 11 compounds present in <em>Lentinus squarrosulus</em> (Mont.,1842) against five key protein targets involved in diabetes pathophysiology α-amylase, α-glucosidase, GLUT4, IRS1, and GLP1 identified naltrindole, an isoquinoline, as the most effective anti-diabetic mycocompound. Naltrindole shows stronger binding affinities to the five target proteins than prescribed drugs acarbose and metformin. The molecular dynamic simulation study performed suggests the strong interactions of the naltrindole-α amylase complex as evidenced by stable root mean square deviation (RMSD) and root mean square fluctuation (RMSF) plots. Hence, this study reports the identification of naltrindole, resourced from <em>Lentinus squarrosulus</em> (Mont.,1842)<em>,</em> a medicinal mushroom, as a promising anti-diabetic drug molecule for the 21st century.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100175"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the bioactive compounds and anticoagulant effects of Bidens pilosa cultivated in yemen through chromatographic and molecular docking approaches","authors":"Bushra Abdulkarim Moharram ,&nbsp;Tareq Al-Maqtari ,&nbsp;Saleh A.M.A. Alomaisi ,&nbsp;Wafa M. Al-Madhagi ,&nbsp;Ibrahim Ali Al-Hakami ,&nbsp;Hassan M. AL-Mahbashi","doi":"10.1016/j.prenap.2025.100174","DOIUrl":"10.1016/j.prenap.2025.100174","url":null,"abstract":"<div><div>Blood coagulation is a rapid and tightly regulated process essential for hemostasis, with disruptions linked to various diseases. This study evaluated the phytochemical composition, total phenolic content (TPC), antioxidant, and anticoagulant activities of <em>Bidens pilosa</em> leaf extract. Chemical constituents were identified using gas chromatography-mass spectroscopy (GC-MS). The TPC was determined spectrophotometrically using the Folin Ciocalteu method. The antioxidant activity of the extract was assessed through the 2.2 – diphenyl −1- picrylhydrazyl (DPPH) free radical scavenging assay. The anticoagulant activity was evaluated via coagulation tests and molecular docking analysis. GC-MS identified methyl palmitate (52.22 %), methyl stearate (29.02 %), and methyl α-linolenate (4.93 %) as major constituents. TPC was 5.94 mg GAE/g, and the extract exhibited strong antioxidant activity (IC₅₀ = 18.2 µg/ml). The extract dose-dependently prolonged clotting time, prothrombin time (PT), and activated partial thromboplastin time (aPTT) in human blood (p &lt; 0.05–0.01). In rabbits, medium doses (50 mg/kg) significantly increased clotting and bleeding times, while higher doses (100 mg/kg) primarily affected bleeding time. Molecular docking confirmed anticoagulant activity, with methyl α-linolenate forming hydrogen bonds with prothrombin. These results highlight <em>B. pilosa</em>'s antioxidant and anticoagulant potential, suggesting its therapeutic application in coagulation disorders.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100174"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Antimicrobial activity of selected native Australian Terminalia spp. against gastrointestinal pathogens and potentiation of selected antibiotics” [Pharmacol. Res. Nat. Prod. 6 (2025) 100158]","authors":"Muhammad Jawad Yousaf Zai ,&nbsp;Matthew James Cheesman ,&nbsp;Ian Edwin Cock","doi":"10.1016/j.prenap.2025.100165","DOIUrl":"10.1016/j.prenap.2025.100165","url":null,"abstract":"","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100165"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}