Pharmacological Research - Natural Products最新文献

{"title":"Assessment of in vitro antioxidant, antidiabetic, anti-inflammatory, and cytotoxicity properties and HPTLC-EDA based phytochemical screening of Macropanax undulatus leaves: An ethnomedicinal plant of Darjeeling Himalayan region","authors":"Ankrita Thapa ,&nbsp;Priya Das ,&nbsp;Tarun Kumar Dua ,&nbsp;Paramita Paul ,&nbsp;Gouranga Nandi ,&nbsp;Sangita Dey ,&nbsp;Anoop Kumar ,&nbsp;Ranabir Sahu","doi":"10.1016/j.prenap.2025.100220","DOIUrl":"10.1016/j.prenap.2025.100220","url":null,"abstract":"<div><div><em>Macropanax undulatus</em>, a plant indigenous to the Eastern Himalayan region has not been studied yet, although the locals claim it to be a valuable medicinal plant. The research focuses on its <em>in vitro</em> pharmacological activities, including antioxidant, antidiabetic, anti-inflammatory, and cytotoxic properties of various extracts from leafy parts. Antioxidant was done by using DPPH, metal chelating, and reducing power assays. α-Amylase and α-glucosidase inhibition assays were used to evaluate antidiabetic potential of the plant extracts. Protein denaturation assay was used to perform anti-inflammatory activity. MTT assay was employed to evaluate the cytotoxicity activity. HPTLC based effected directed analysis (EDA) was used to identify antioxidant and antidiabetic agents. Extracts, other than pet. ether and <em>n</em>-hexane extracts, showed high TPC where all extracts showed better TFC. Methanolic (IC₅₀:77.22 ± 0.53 µg/mL) and hydroalcoholic extracts (IC₅₀:75.94 ± 5.82 µg/mL) were observed to possess prominent antioxidant property. The plant also exhibited significant antidiabetic (hydroalcoholic, IC₅₀: 181.90 ± 15.44 µg/mL), as well as anti-inflammatory properties (aqueous, IC₅₀: 91.96 ± 6.03 µg/mL). Aqueous extract showed highest cytotoxicity properties among the other extracts. Further, the chemical composition of those extracts was evaluated with HPTLC based EDA which revealed the presence of stigmasterol and gallic acid in leaves extracts in qualitative as well as in quantitative manner. These results suggest that <em>M. undulatus</em> may have therapeutic potential for the treatment of oxidative damage, diabetes, anti-cancer and inflammatory illnesses. Presence of gallic acid and stigmasterol may be responsible for the therapeutical properties of the plant.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100220"},"PeriodicalIF":0.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the anti-infective potential of phytoconstituents derived from Euphorbia neriifolia Linn.: Molecular docking, pharmacokinetics, drug-likeliness, and toxicological evaluations","authors":"Md. Jamal Hossain ,&nbsp;Md. Shohel Hossen ,&nbsp;Md Anisur Rahman ,&nbsp;Md. Hosan Khan ,&nbsp;Joy Johon Roy ,&nbsp;Hujjout Ullah ,&nbsp;Kazi Md. Asraful Islam ,&nbsp;Morium Benta Mohasin ,&nbsp;Mst. Monisha Akter Mimi ,&nbsp;Marsia Haque Meghla ,&nbsp;Md. Rejaul Karim Bhuiya ,&nbsp;Utsha Barua ,&nbsp;Mohammad A. Rashid","doi":"10.1016/j.prenap.2025.100219","DOIUrl":"10.1016/j.prenap.2025.100219","url":null,"abstract":"<div><h3>Background and aim</h3><div>Growing resistance to conventional antibiotics has promoted interest in bioactive molecules derived from plants that may have anti-infective effects. <em>Euphorbia neriifolia</em> has long been used for its therapeutic benefits, exhibited potential as a source of antimicrobial natural products. The present research aimed to investigate the molecular interactions of several lead compounds of <em>E. neriifolia</em> focusing on antimicrobial effects, pharmacokinetics and toxicological properties, including their drug-likeliness profiles.</div></div><div><h3>Methods</h3><div>A total of 18 lead compounds of <em>E. neriifolia</em> were retrieved from the literature based on their antimicrobial properties. PyRx, PyMol, and Discovery Studio (v4.5) were used for molecular docking against CTX-M-9 Beta-lactamase, dihydrofolate reductase enzyme, DNA gyrase, and OmpF Porin for antibacterial properties; 3CL protease, RNA dependent RNA polymerase (RdRp), and spike protein for anti-SARS-CoV-2 potential; neuraminidase and hemagglutinin H1 for anti-influenza; old yellow enzyme for antifungal; and HIV-1 reverse transcriptase for anti-HIV activities. Absorption, distribution, metabolism, excretion, and toxicity (ADME/T) with drug likeliness properties were analyzed using pkCSM and Swiss ADME online tools.</div></div><div><h3>Results</h3><div>Among the 18 compounds, rutin and tulipanian exhibited higher binding affinities against most targets than their corresponding standard ligands. Most compounds, including rutin, friedelin, lupenone, beta-amyrin, and tulipanin, showed OmpF Porin protein inhibitory properties. At least 10 compounds, including quercetin, rutin, tulipanin, afzelin, pachypodol, and euphonerin A-D, exhibited higher binding affinities against the fungal old yellow enzyme, revealing promising antifungal properties. Furthermore, most compounds exerted favorable ADME/T properties and showed drug likeliness by obeying the Lipinski rule of five.</div></div><div><h3>Conclusion</h3><div>Based on the current findings, this study offers the possibility of making a natural remedy for treating microbial infections from <em>E. neriifolia</em>. These results can be considered as the groundwork for developing plant-based medicinal compounds and conducting further <em>in silico</em> and experimental validation.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100219"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Aegle marmelos (L.) Corrêa extracts in the management of breast cancer: Phytochemical profiling and molecular docking studies","authors":"Nehal Rami ,&nbsp;Keyur Bhatt","doi":"10.1016/j.prenap.2025.100218","DOIUrl":"10.1016/j.prenap.2025.100218","url":null,"abstract":"<div><h3>Background</h3><div>Breast cancer is the most prevalent cancer among women and a leading cause of cancer-related fatalities worldwide. Addressing the ongoing challenges of drug resistance, alongside the need for effective, affordable prevention and treatment strategies, is essential for reducing its impact.</div></div><div><h3>Objective</h3><div>This study evaluates the anticancer potential of <em>Aegle marmelos</em> (L.) Corrêa leaf extracts through in vitro assays and in silico molecular studies.</div></div><div><h3>Methods</h3><div>Hexane, methanol, and aqueous extracts of <em>Aegle marmelos</em> (L.) Corrêa leaves were screened for phytochemical composition. Antioxidant potential was assessed using the DPPH assay, while anticancer activity against MCF-7 breast cancer cells was evaluated using the MTT assay. The most potent extract underwent GC-MS and LC-MS analyses to identify bioactive compounds. Selected phytochemicals were subjected to molecular docking studies against Human Estrogen Alpha receptor (PDB ID: 3ERT) and Epidermal Growth Factor Receptor (PDB ID: 2J5F), followed by molecular dynamics simulations to assess complex stability. Toxicity predictions were conducted to evaluate the safety profile of lead compounds.</div></div><div><h3>Results</h3><div>Phytochemical analysis confirmed the presence of bioactive compounds, including flavonoids, phenols, and phytosterols. The methanol extract showed strong antioxidant activity (IC50:31.82 ± 0.46 µg/mL), while the hexane extract demonstrated significant cytotoxicity against MCF-7 cells (IC50:24.27 ± 2.39 µg/mL). GC-MS and LC-MS analyses identified 22 bioactive compounds, among which Caryophyllene oxide emerged as a promising therapeutic candidate due to its strong binding affinity for both Human Estrogen Alpha receptor (PDB ID: 3ERT) and Epidermal Growth Factor Receptor (PDB ID: 2J5F). Molecular dynamics simulations confirmed its stable interactions, and toxicity assessments indicated a favorable safety profile, reinforcing its potential for breast cancer treatment.</div></div><div><h3>Conclusion</h3><div>These findings highlight the potential of <em>Aegle marmelos</em> (L.) Corrêa leaf extracts as a natural anticancer agent, with Caryophyllene oxide emerging as a promising lead compound due to its strong binding affinity, stability, and favorable safety profile. This study provides a foundation for further preclinical and clinical investigations to explore its therapeutic potential for breast cancer treatment.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100218"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of marine macro algal-derived compounds as antiviral drugs: A review on the types, mechanisms of action, and challenges","authors":"Yuvaraj Dinakarkumar ,&nbsp;Gnanasekaran Ramakrishnan ,&nbsp;Janaki Ramaiah Mekala ,&nbsp;Mallu Maheshwara Reddy ,&nbsp;Rinish Mortin John ,&nbsp;Aishwarya Lakshmi Thasvanth Raj ,&nbsp;Sahiti Chamarthy","doi":"10.1016/j.prenap.2025.100215","DOIUrl":"10.1016/j.prenap.2025.100215","url":null,"abstract":"<div><div>The rise of viral diseases has prompted significant interest in marine natural products due to their diverse biological activities. Seaweeds, in particular, have emerged as abundant sources of bioactive compounds, including potent antiviral agents, alongside other therapeutic properties. This review aims to explore the bioactive compounds present in seaweeds and their efficacy against viruses. It also seeks to discuss these compounds' extraction and isolation methodologies, highlighting various techniques involved. Furthermore, it endeavors to address the challenges and opportunities in seaweed research, shaping the future of investigations in this area. This review analyses existing literature on the bioactive compounds derived from seaweeds and their antiviral properties. It evaluates extraction and isolation techniques employed in obtaining these compounds, providing insights into methodologies utilized in their study. Additionally, it analyzes challenges and opportunities in seaweed research, offering perspectives on future directions in the field. Seaweeds harbor a diverse array of bioactive compounds with significant potential in combating viral diseases. The exploration of these compounds and their antiviral efficacy provides valuable insights into novel therapeutic avenues. The field of seaweed research promises exciting opportunities for the development of new antiviral treatments and underscores the importance of continued exploration in this area.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100215"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of co-administering the flavonoids quercetin and biochanin A on simvastatin toxicity in HepG2 cultures","authors":"Joshua Zechner ,&nbsp;Susan M. Britza ,&nbsp;Rachael Farrington ,&nbsp;Roger W. Byard ,&nbsp;Ian F. Musgrave","doi":"10.1016/j.prenap.2025.100209","DOIUrl":"10.1016/j.prenap.2025.100209","url":null,"abstract":"<div><h3>Aims</h3><div>Inhibition of hepatic organic anion transporter polypeptide 1B1 (OATP1B1) or cytochrome 3A4 (CYP3A4) via pharmacokinetic interactions is known to increase the risk of simvastatin-induced myopathy. The flavonoids quercetin and biochanin A are known to inhibit both OATP1B1 and CYP3A4, however it is unknown whether co-administration of these flavonoids with simvastatin is likely to lead to myopathy. Thus, quercetin and biochanin A were co-administered with simvastatin to investigate whether hepatic absorption or metabolism was inhibited.</div></div><div><h3>Main methods</h3><div>The hepatic carcinoma cell line, HepG2 cells was used to model pharmacokinetic interactions. CYP3A4 activity in the HepG2 cultures was confirmed through rifampicin (40 µM) pre-treatment inducing paracetamol (20–50 mM) toxicity. OATP activity in HepG2 cultures was validated using the fluorescent probe pyranine (0–50 µM), followed by inhibition of specific pyranine uptake by the flavonoids quercetin and biochanin A and the drugs gemfibrozil and diltiazem. Toxicity screenings were performed using an MTT assay for simvastatin (20–50 µM) in the absence or presence of diltiazem, gemfibrozil, quercetin and biochanin A (40 µM).</div></div><div><h3>Key findings</h3><div>Quercetin and biochanin A (40 µM) inhibited specific pyranine uptake comparable to the known OATP inhibitor gemfibrozil (40 µM) (n = 4, P ≤ 0.05). 40 µM of gemfibrozil significantly reduced simvastatin toxicity at 20 µM (n = 4, P ≤ 0.05). Quercetin and diltiazem (40 µM) did not modulate simvastatin toxicity. Biochanin A at 40 µM significantly induced simvastatin toxicity at concentrations 20–50 µM (n = 4, P ≤ 0.05).</div></div><div><h3>Significance</h3><div>Quercetin and biochanin A inhibited OATP, possibly implicating them in drug-drug interactions, but simvastatin toxicity in HepG2 cells was not attenuated as initially hypothesised. Biochanin A significantly synergistically increased simvastatin toxicity in HepG2 cultures, which warrants further studies.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100209"},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophilic fraction of Selaginella convoluta (Arn.) Spring shoots extract: Chemical characterization and embryotoxicity in zebrafish (Danio rerio)","authors":"Luiz Fernando Rodrigues de Souza ,&nbsp;Fernanda Priscila Santos Reginaldo ,&nbsp;João Luiz Bronzel Júnior ,&nbsp;Ana Carolina Luchiari ,&nbsp;Raquel Brandt Giordani","doi":"10.1016/j.prenap.2025.100216","DOIUrl":"10.1016/j.prenap.2025.100216","url":null,"abstract":"<div><div>As part of our ongoing efforts to better comprehend the species <em>Selaginella convolute</em> (Arn.) Spring, a desiccation-tolerant plant native to the semiarid Caatinga biome, this study aimed to investigate the chemical composition and safety of the hydrophilic fraction (SHF) derived from their shoots extract using the embryotoxicity test in an <em>in vivo</em> model. UPLC-MS/MS analysis revealed an array of phenolic compounds, such as biflavonoids, selaginellins and hydroxycinnamic derivatives. Some, such as selaginpulvilin A and seladoeflavone, are being reported for the first time in <em>S. convoluta</em>. Embryos exposed to concentrations of 2.5, 5.0, and 10.0 mg/ml of the SHF exhibited no signs of toxicity or teratogenicity to zebrafish embryos during the observation period. No mortality was observed among embryos exposed to any concentration of the SHF. However, continuous exposure at a concentration of 10 mg/ml resulted in mortality after 4 hours, with a survival rate below 50 % at 16 hours. The study confirmed the low toxic potential of the SHF during acute exposure and the absence of teratogenic effects across the tested concentrations. A hundred metabolites were detected and just 6.5 % could be annotated regarding online and in-house database. The intriguing chemo diversity of the SHF makes it a promising resource for discovering new chemical entities and developing novel drugs. Moreover, the fraction demonstrates a low toxic potential upon acute exposure <em>in vivo</em>, enhancing its potential for pharmaceutical applications which could drive a biomonitorated isolation to achieve an unequivocal structural assignment.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100216"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of pectin and its vital role in the bioavailability of phytochemicals associated with therapeutic properties","authors":"Venkatesh Kumar R.,&nbsp;Devika Srivastava,&nbsp;Abhishek Verma,&nbsp;Akash Mishra","doi":"10.1016/j.prenap.2025.100214","DOIUrl":"10.1016/j.prenap.2025.100214","url":null,"abstract":"<div><div>Pectin is a complex and multifunctional heteropolysaccharide that plays a significant role in various industries, including food, pharmaceuticals, and cosmetics. This review comprehensively explores the biosynthesis of pectin, highlighting its formation in plants and subsequent digestion in the form of fermentation, primarily through fermentation by the gut microbiome. Furthermore, the role of pectin in enhancing the bioavailability of plant-derived nutraceuticals is discussed, emphasizing its potential as a delivery vehicle for bioactive compounds. The paper also examines the wide-ranging biological activities of pectin, including its anticancer, antidiabetic, cardioprotective, hepatoprotective, neuroprotective, anti-inflammatory, and antioxidant properties. By integrating current research, this review provides a holistic understanding of pectin's multifaceted roles in human health, positioning it as a promising functional food ingredient and therapeutic agent.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100214"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antidiabetic potential of Ceiba pentandra bark powder: Chemical characterization and bioactivity of a new stigmastane","authors":"Jounda Nadège Nelly ,&nbsp;Nouga Bissoué Achille ,&nbsp;Assiéné Agamou Julien Armel ,&nbsp;Happi Nguefa Emmanuel","doi":"10.1016/j.prenap.2025.100217","DOIUrl":"10.1016/j.prenap.2025.100217","url":null,"abstract":"<div><div>Ethnopharmacological relevance: The management of diabetic patients (DP) with conventional drugs is increasingly being replaced by plant extracts. Among these plants, the bark of <em>Ceiba pentandra</em> (CP) is known for its antidiabetic activities due to its bioactive compounds (BC), the chemical characteristics of which are not fully identified. The objective of this study was to determine the chemical and structural characteristics of the compounds isolated from CP bark powder extracts, as well as those exhibiting the best antidiabetic activity. The bark of CP was harvested and processed into powders. The structures of the chemical compounds isolated from the methanolic extracts of CP bark powder were determined, and the inhibitory activities against digestive enzymes (alpha-amylase, alpha-glucosidase, beta-galactosidase) and antioxidant activities of the extracts and chemical compounds were evaluated. It was found that of the eight identified BC, seven are known and one, belonging to the stigmastane class (phytosterol), with a chemical structure corresponding to stigmastane-1,3,5-triol, is a new compound. This new compound, unlike the extracts of CP, exhibited a higher antihyperglycemic potential than others, including acarbose, and a higher antioxidant activity linked to iron-reducing power (FRAP) than vitamin C. The knowledge of the antidiabetic potential of stigmastane-1,3,5-triol is important in the use of CP bark powders for the management of blood glucose levels in diabetic patients and may contribute to the development of an improved traditional medicine.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100217"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lutein abates doxorubicin-induced testicular toxicity via modulation of Beclin-1/mTOR signaling pathway mediating inhibition of apoptosis, inflammation and oxidonitrergic stress","authors":"Jennifer Efe Jaiyeoba-Ojigho ,&nbsp;Jerome Ndudi Asiwe ,&nbsp;Blessing Zeinab Ovili-Odili ,&nbsp;Taniyohwo Mamerhi Enaohwo ,&nbsp;Lilian Ebele Chris-Ozoko ,&nbsp;Alexander Obidike Naiho ,&nbsp;Emmanuel Ikechukwu Okolie ,&nbsp;Blessing Ngozi Nwanneka ,&nbsp;Mercy Jesuovotekevwe Aghale ,&nbsp;David Osaze Isehrenhren ,&nbsp;Greatman Nelson Akotonou ,&nbsp;Annie Aiweruosuoghene Ogboru","doi":"10.1016/j.prenap.2025.100210","DOIUrl":"10.1016/j.prenap.2025.100210","url":null,"abstract":"<div><h3>Background</h3><div>Doxorubicin (DOX) is a medication utilized in several solid tumor treatment. However, using it raises the possibility of serious dose-dependent injury to non-target organs such as the testis. Meanwhile, research has shown that the naturally occurring carotenoid lutein, has androgenic, anti-inflammatory and antioxidant properties. It is unclear, though, if lutein can lessen the damage that doxorubicin causes on the testicles. Therefore, the purpose of this study was to determine how lutein ameliorated doxorubicin-induced testicular toxicity in male Wistar rats.</div></div><div><h3>Methods</h3><div>Animals were randomly assigned to four groups and treated with 10 ml, of saline, 15 mg/kg of doxorubicin, 40 mg/kg of lutein and DOX with lutein, respectively. Treatment waas done intraperitoneally for 28 days. Hormonal assay, androgenic enzyme quantification accompanied with antioxidant, apoptotic players, pro-inflammatory cytokine and autophagy mediator assays were done using UV/VIS spectrophotometry, ELISA and histological techniques.</div></div><div><h3>Results</h3><div>The results showed that doxorubicin caused a dysfunctional pituitary-testicular hormonal axis accompanied with low sperm count and semen quality. Also, oxidative stress leading to activation of autophagy which was accompanied with inflammation, apoptosis and fibrosis were all associated with doxorubicin-induced testicular toxicity. However, treatment with lutein significantly abated these changes and restored normal testicular functions.</div></div><div><h3>Conclusion</h3><div>Lutein abated doxorubicin-induced testicular toxicity via modulation of Beclin-1/mTOR signaling pathway mediating inhibition of apoptosis, inflammation and oxidonitrergic stress.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100210"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of antihypertensive and hypocholesterolemic effect of white lupine (Lupinus albus L.): A review","authors":"Kibur Hunie Tesfa ,&nbsp;Asrat Tadele Ewunetie ,&nbsp;Chernet Desalegn Gebeyehu","doi":"10.1016/j.prenap.2025.100207","DOIUrl":"10.1016/j.prenap.2025.100207","url":null,"abstract":"<div><div>Lupine is a legume that is the main source of plant-derived protein in human nutrition. The high protein content of the lupine seed is a foundation of bioactive peptides. The biological actions of lupine protein and peptides include hypocholesterolemic, hypoglycemic, antibacterial, immunomodulatory, and anti-inflammatory effects. <em>Lupinus albus L.</em> seeds induce vasorelaxation by stimulating the endothelial nitric oxide synthase-nitric oxide-cyclic guanosine monophosphate pathway and directly inhibiting voltage-dependent calcium channels. By acting on the renin-angiotensin system, blood pressure is regulated. And, peptides with this potential are called angiotensin-converting enzyme inhibitory peptides. Lupine protein hydrolysate induces complementary cholesterol-lowering effects by inhibiting 3-hydroxy-3-methylglutaryl CoA reductase activity, the contact between proprotein convertase subtilisin/kexin type 9 and the low-density lipoprotein receptor. This dual influence controls both the proprotein convertase subtilisin/kexin type 9 and the low-density lipoprotein receptor signaling pathways. This facilitates hypocholesterolemic effects of lupine peptides and inhibits the activity of 3-hydroxy-3-methylglutaryl CoA reductase leading to upregulation of the low-density lipoprotein receptor protein levels by activation of the sterol regulatory element binding protein 2 pathways. In this article, we attempt to address various health benefits that lupine brings and the mechanistic pathway by which lupine protein takes action.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100207"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}