Pharmacological Research - Natural Products最新文献

{"title":"Evaluation of wound healing potential of lawsone from Lawsonia innermis and its organometallic complexes in rats","authors":"Purnima V ,&nbsp;Chhaya Harihar Gadgoli","doi":"10.1016/j.prenap.2024.100035","DOIUrl":"10.1016/j.prenap.2024.100035","url":null,"abstract":"<div><h3>Aim of the study</h3><p>To study the utility of Lawsone along with its zinc and copper complexes for wound healing activity using excision and incision wound models in rats.</p></div><div><h3>Methods</h3><p>The complex of plant nathoquinone from <em>Lawsonia inermis</em> called Lawsone viz Law and the metals viz. copper and zinc were synthesized. Molecular Docking (with GSK 3β protein) and Molecular Dynamic studies were carried out. The wound healing activity was evaluated using excision and incision wound models in Albino Wistar rats. The six groups comprised disease control, vehicle control, and standard, groups treated with Lawsone (Law) and its Zn Complex (ZnLaw) and copper complex (CuLaw). The progress of excision wound healing was monitored through wound contraction and biochemical and antioxidant parameters along with histopathology of granulation tissue, while for incision wounds, breaking and tensile strength were determined.</p></div><div><h3>Results</h3><p>The molecular docking studies revealed that ZnLaw has the highest affinity with GSK 3β protein. The Molecular Dynamic study determined the stability of Law and its metal complexes with GSK 3β protein and was found to be stable in the environment. The test compounds Law, ZnLaw, and CuLaw displayed significant (p&lt;0.05) wound contraction. There was a significant (P&lt; 0.01) decrease in the period of epithelisation as compared to vehicle control. ZnLaw showed maximum reduction in the period of epithelisation (16.03±0.942). There was a significant improvement in the antioxidant parameters from the granulation tissues of wounds treated with Law, ZnLaw, and CuLaw. The metal complexes of Law viz. ZnLaw and CuLaw showed a remarkable increase in hydroxyproline and hexosamine content as compared to the control. Histopathological findings indicated re-epithelisation, neovascularization, and increased collagen deposition as compared to the disease control. The incision model also revealed a significant (P&lt; 0.001) increase in the tensile strength and breaking strength of the healed tissue as compared to the control.</p></div><div><h3>Conclusion</h3><p>Law and its Zinc and copper complexes exhibited good potential in healing of both incision and excision wounds.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140398983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and bioavailability studies on orodispersible tablets containing standardized extracts of Glycyrrhiza glabra, Curcuma longa and Piper nigrum","authors":"Chhaya Gadgoli,&nbsp;Archana Naik,&nbsp;Kavya Bangera,&nbsp;Shweta Joshi","doi":"10.1016/j.prenap.2024.100032","DOIUrl":"10.1016/j.prenap.2024.100032","url":null,"abstract":"<div><h3>Background</h3><p>Glycyrrhizin, Curcumin and Piperine indicated good antiasthamatic properties owing to their ability to inhibit airways constriction and anti-inflammatory properties. Orodispersible tablets have advantage of faster drug release and has patient compliance in asthmatic patients.</p></div><div><h3>Hypothesis / Purpose</h3><p>To develop an orodispersible polyherbal tablets using the standardized extracts of <em>Glycyrrhiza glabra, Curcuma longa and Piper nigrum</em> with respect to glycyrrhizin, curcumin and piperine respectively and evaluate bioavailability of the markers from the formulation.</p></div><div><h3>Methods</h3><p>Alcoholic extracts of <em>G. glabra, C. longa,</em> and <em>P. nigrum</em> were standardized using HPTLC for glycyrrhizin, curcumin, and piperine content, respectively. The orodispersible tablet formulation was created and standardized for curcumin (50 mg), glycyrrhizin (75 mg), and piperine (2.5 mg) per tablet, using the HPTLC method. The absolute Bioavailability studies were conducted for glycyrrhetinic acid and curcumin in rats by administering pure marker compounds intravenously (2 mg/kg b.w) and orally (10 mg/kg b.w.), followed by determining relative bioavailability through oral administration of the orodispersible tablet formulations.</p></div><div><h3>Results</h3><p>Orodispersible tablets could be successfully developed using the standardized extracts. The HPTLC bioanalytical method was developed and validated as per M 10 guideline and results were in accordance to specifications. The bioavailability studies in rats indicated absolute bioavailability of Glycyrrhetinic acid and Curcumin to be 20.35 and 2.57% respectively and relative bioavailabilities of orodispersible formulation F1 were found to be 75 and 38.67%.</p></div><div><h3>Conclusion</h3><p>A standardized orodispersible formulation can be a better option for asthma patients. The low absolute bioavailability of curcumin was significantly (P&lt;0.05) increased in both the formulations F1 and F2. This may be attributed to the extracts containing saponin glycosides like Glycyrrhizin aiding into greater dissolution of curcumin. Formulation with piperine (F1) showed significant increase in bioavailability is due to inhibition of human P-glycoprotein, cytochrome P450 3A4 (CYP3A4) and increase secretion of bile acids.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of essential oils from Eucalyptus camaldulensis Dehn. and Eucalyptus tetragona F.Muell","authors":"Abdulaziz Abrahim ,&nbsp;Muhidin Tahir ,&nbsp;J.M. Sasikumar ,&nbsp;Misrak Kebede ,&nbsp;Meseret C. Ejigu ,&nbsp;Amin Mohammed Yones","doi":"10.1016/j.prenap.2024.100033","DOIUrl":"10.1016/j.prenap.2024.100033","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Eucalyptus</em> plants are used as a source of essential oils in Ethiopia and are traditionally used for the treatment of the common cold, stomachache, swelling and wounds. This study aims to determine the chemical composition and antibacterial activity of the essential oils of <em>E. camaldulensis</em> Dehn. and <em>E. tetragona</em> Muell.</p></div><div><h3>Methodology</h3><p>The chemical composition of the essential oils of <em>E. camaldulensis</em> Dehn. and <em>E. tetragona</em> F.Muell. was determined using gas chromatography<img>mass spectrometry (GC<img>MS), and their antibacterial activity of was evaluated against <em>E. coli</em> ATCC 25922 and <em>S.</em> ATCC 25923.</p></div><div><h3>Results</h3><p>The yield of essential oil from the leaves of <em>E. camaldulensis</em> was 0.72% w/w, and the yield of essential oil from the leaves of <em>E. tetragona</em> was 0.80% w/w on the basis of fresh leaf weight. A total of 17 and 14 components were identified in <em>E. camaldulensis</em> and <em>E. tetragona,</em> respectively. The main components identified were 1,8-cineole, β-cymene and α–pinene in both <em>Eucalyptus</em> species analysed. The extracted essential oils displayed moderate to strong antibacterial activity. <em>E. tetragona</em> inhibited <em>S. aureus</em> at a lower concentration (0.625 μg/ml), while <em>E. coli</em> was less sensitive to the EOs of <em>E. camaldulensis</em>, with an MIC of 2.5 μg/ml.</p></div><div><h3>Conclusion</h3><p>The essential oils of <em>E. camaldulensis</em> and <em>E. tetragona</em> exhibited significant antibacterial activity against <em>E. coli</em> and <em>S. aureus</em>. The results showed that the essential oils of <em>E. camaldulensis</em> and E. <em>tetragona</em> may be useful for treating various infectious diseases. Essential oils, particularly 1,8-cineole, can be used as alternative natural antimicrobial agents in the pharmaceutical and food industries.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140278986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical characterization, in vivo anti-oxidant, anti-inflammatory and anti-apoptotic properties of Peperomia pellucida in modulating vascular tone in Swiss mice exposed to aluminium chloride","authors":"Jerome Ndudi Asiwe ,&nbsp;Godwin D. Yovwin ,&nbsp;Vincent-junior Onoriode Igben ,&nbsp;Blessing Abe ,&nbsp;Sokiprim Akoko ,&nbsp;Benjamin Oritsemuelebi ,&nbsp;Joseph Chimezie ,&nbsp;Anthony Emeka Ojieh ,&nbsp;Gwendolin Dagogo","doi":"10.1016/j.prenap.2024.100034","DOIUrl":"https://doi.org/10.1016/j.prenap.2024.100034","url":null,"abstract":"<div><h3>Background</h3><p>Regulation of balance between vasoconstriction and vasodilation has become a therapeutic target to vascular complications. However, Aluminium chloride, a naturally abundant environmental toxicant has been reported to disrupt this balance via increased oxidative stress and inflammation in several studies. Whether <em>Peperomia pellucida</em>, a popular medicinal plant known for its pharmacological benefits can modulate vascular tone in mice exposed to aluminium chloride is yet to be unravelled. This study investigated the effect of <em>Peperomia pellucida</em> ethanol leaf extract on aluminium chloride induced vascular dysfunction in male Swiss mice.</p></div><div><h3>Methods</h3><p>Phytochemical profiling of <em>Peperomia pellucida</em> was done using GC-MS and vascular effect of <em>Peperomia pellucida</em> was investigated by administering 50 mg and 100 mg of the extract alongside aluminium chloride orally for 14 days. Aorta was harvested for biochemical (SOD, CAT, GSH, MDA, nitrites), ELISA (endothelin-1, TNF-α and IL-6) assays as well as immunohistochemical studies (occludin and caspase-3).</p></div><div><h3>Results</h3><p>GC-MS profiling showed 8 phytocompounds with 3,5-Dimethoxybenzamide as the highest peak area. Antioxidant armouries such as SOD catalase, glutathione as well as vascular occludin expression was significantly enhanced by <em>Peperomia pellucida</em> (50 mg/kg and 100 mg/kg) against aluminium chloride perturbations while suppressing TNF-α, IL-6, ET-1 activities and caspase-3 expression against increase caused by aluminium chloride intoxication.</p></div><div><h3>Conclusion</h3><p>Our result showed that <em>Peperomia pellucida</em> leaf extract modulated vascular tone via antioxidant, anti-inflammatory and ant-apoptotic properties which was mediated by its phytocompounds</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140191554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caffeic acid attenuates acrylamide induced biochemical, hematological, and histological alterations in rats","authors":"Divya Gupta,&nbsp;Sadhana Shrivastava,&nbsp;Shamli S. Gupte,&nbsp;Sangeeta Shukla","doi":"10.1016/j.prenap.2024.100031","DOIUrl":"10.1016/j.prenap.2024.100031","url":null,"abstract":"<div><p>Acrylamide, a ubiquitous industrial chemical, and food contaminant, has been implicated in various toxicological effects, including biochemical, hematological, and histological alterations. In this study, we investigated the potential protective effects of caffeic acid against acrylamide-induced toxicity in a rat model. Rats were divided into three different groups, including a control group, an acrylamide-exposed group (19.13 mg/kg), and groups treated with acrylamide (19.13 mg/kg for 28 days) + caffeic acid (20 mg/kg after 28 days exposure to acrylamide). Biochemical parameters, hematological indices, and histopathological changes were systematically evaluated to assess the impact of acrylamide and the ameliorative effects of caffeic acid. Our results revealed that acrylamide exposure led to significant alterations in biochemical markers, hematological and serological parameters, and histological architecture in liver, kidney, and brain tissues. However, administration of caffeic acid demonstrated attenuation of these adverse effects. The protective effects of caffeic acid were evident through the restoration of key biochemical parameters, maintenance of hematological and serological homeostasis, and preservation of tissue histology. Furthermore, our findings suggest that caffeic acid may exert its protective effects through antioxidant mechanisms, as evidenced by the reduction in oxidative stress markers. In conclusion, this study provides comprehensive insights into the protective potential of caffeic acid against acrylamide-induced biochemical, hematological, serological, and histological alterations in rats. The observed ameliorative effects underscore the therapeutic potential of caffeic acid as a promising adjunct in mitigating the toxicological consequences of acrylamide exposure.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and enzyme inhibitory potentials of phytochemicals isolated from Dioclea reflexa (Hook F.) stem: in-vitro and in-silico studies","authors":"Abdulkabir Oladele Oladimeji ,&nbsp;Solomon Oluwaseun Akinnawo ,&nbsp;Damilola Alex Omoboyowa ,&nbsp;Mehreen Lateef","doi":"10.1016/j.prenap.2024.100030","DOIUrl":"10.1016/j.prenap.2024.100030","url":null,"abstract":"<div><p>The objective of the study was to isolate and characterize phytochemicals from the stem extract of <em>D. reflexa</em> and investigate their biochemical activities. Five flavonoids together with two terpenoids, were isolated. Their structures were identified using 1D and 2D nuclear magnetic resonance (NMR) and mass spectroscopic techniques in combination with circular dichroism (CD) spectroscopy, as well as comparisons with published data. The <em>in vitro</em> reactive oxygen species scavenging potential of the compounds was investigated. The lipoxygenase and urease inhibitory potential of the compounds were tested using <em>in vitro</em> and <em>in silico</em> methods. Compound <strong>1</strong> and <strong>2</strong> exhibited potent antioxidant activity with lower IC₅₀ values of 38.5 ± 0.25 and 39.5± 0.14 μM respectively compared with the standard used (BHA IC₅₀ value = 39.5± 0.14) in DPPH assay. Compound <strong>1</strong> and <strong>2</strong> also exhibit good reducing ability and superoxide radical scavenging activity reflected by IC₅₀ value 44.6 ± 0.30 and 48.5 ± 0.16 respectively compared with BHA (IC₅₀ 45.6 ± 0.54). Compound <strong>2</strong> and <strong>3</strong> showed significant inhibitory effect against urease with IC₅₀ values 26.2 ± 0.29 μM and 36.5 ± 0.37 μM respectively, while compound <strong>4</strong> with IC₅₀ value 33.3 ± 0.74 μM is the most active against lipoxygenase. All the seven compounds showed varying degrees of binding affinity against the targets with compounds 1–5 having the better docking score compared with the co-crystalized ligands of both lipoxygenase and urease. The density functional theory reveals the replicate of the transitional state between the compounds and their respective receptor and stability of the compounds was predicted from the energy gap (E_LUMO – E_HOMO). These compounds are potential drug against stomach ulcers, inflammation and oxidative stress associated diseases. It is also worth mentioning that the complete assignments of NMR data 1 and 2 were reported for the first time in this study.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140276035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanolic extract of Cleome droserifolia mitigates epinephrine-induced cardiac injury","authors":"Sahar A. Abou Halek ,&nbsp;Hanan M. Rashwan ,&nbsp;Hala M. Ebaid ,&nbsp;Heba M.A. Abdelrazek ,&nbsp;Heba N. Gad El Hak","doi":"10.1016/j.prenap.2024.100028","DOIUrl":"10.1016/j.prenap.2024.100028","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><p><em>Cleome droserifolia</em> treatment has potent antioxidant, antimicrobial, and immunomodulatory potentials, which can help recover the general health status.</p></div><div><h3>Aim of the study</h3><p>This research aims to estimate the potential cardioprotective properties of the methanolic extract <em>of Cleome droserifolia</em> (MEC) against epinephrine-induced cardiac injury (MI) in rats.</p></div><div><h3>Materials and methods</h3><p>Thirty-six male Wistar rats were separated equally into 6 groups. The control group (Cont) received oral distilled water for 30 uninterrupted days and was administered subcutaneous saline on the 31^st and 32^nd days. The MEC 100 group received MEC 100 mg/kg, P.O., for 30 uninterrupted days and administered subcutaneous saline on the 31^st and 32^nd days. The MEC 200 group received MEC 200 mg/kg, P.O. for 30 uninterrupted days and subcutaneous saline was administered on the 31^st and 32^nd days. The epinephrine group (EP) received distilled water orally for 30 uninterrupted days and administered subcutaneous epinephrine (2 mg/kg, s.c.) divided into two doses (1 mg/kg, s.c) each on the 31^st and 32^nd days. MEC 100+Ep group received MEC 100 mg/kg, P.O., for 30 uninterrupted days and administered subcutaneous epinephrine (2 mg/kg, s.c.) divided into two doses (1 mg/kg, s.c) each on the 31^st and 32^nd days. The MEC 200+EP received MEC 200 mg/kg, P.O. for 30 uninterrupted days and administered subcutaneous epinephrine (2 mg/kg, s.c.) divided into two doses (1 mg/kg, s.c) each on the 31^st and 32^nd days. Electrocardiography (ECG), biochemical, oxidative stress, inflammatory mediators, histopathological, immunohistochemical analysis and the expression of phosphoinositide 3-kinases (PI3K) and protein kinase B (AKT) were assessed.</p></div><div><h3>Results</h3><p>MEC reversed epinephrine-induced lessening of heart rate, prolonged QT interval and ST displacement. Pretreatment <em>with</em> MEC significantly reduced serum aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) levels in MI rats. The MEC pretreatment promoted total antioxidant capacity (TAC), glutathione (GSH), and total oxidant status (TOS). In addition, it reduced interleukin-1β (IL-1β), interleukin-6 (IL-6), malondialdehyde (MDA), and tumour necrosis factor (TNF-α) in MI rats. Furthermore, MEC statistically reduced immunohistochemical promotion of nuclear factor–κB (NF-κB) and expression of AKT and PI3K in cardiac tissue and perfected histopathological changes.</p></div><div><h3>Conclusion</h3><p>The MEC, rich in phenolic and flavonoid contents, had a cardioprotective effect on rats suffering from epinephrine-induced MI. Such effect was achieved via improving cardiac function, antioxidant status, attenuating ECG pattern and histological picture, as we","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140278487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioassay guided triterpene isolation and its biological evaluation using branches extract of a significant medicinal plant; Monotheca buxifolia","authors":"Arfah Naveed Dar ,&nbsp;Javeria Shahzad ,&nbsp;Joham Sarfraz Ali ,&nbsp;Usama Sarwar ,&nbsp;Anila Sajjad ,&nbsp;Muhammad Zia","doi":"10.1016/j.prenap.2024.100026","DOIUrl":"https://doi.org/10.1016/j.prenap.2024.100026","url":null,"abstract":"<div><h3>Objective</h3><p>The present study focuses on column chromatography of ethyl acetate fractions and re fractions of <em>M.buxifolia</em> branches for potential compound isolation alongside their biological and phytochemical evaluation and TLC analysis.</p></div><div><h3>Methods</h3><p>The obtained EtOAc fractions were subjected to phytochemical (TPC, TFC), antioxidant (TRP, TAC, DPPH, MC, ABTS), antibacterial and enzyme inhibition (α-amylase, lipase and urease) assays. Fractions indicating the presence of a compound were selected for running successive columns and their factions also analyzed employing the above mentioned bioassays.</p></div><div><h3>Results</h3><p>A collective none-to-low antibacterial activity was observed. MbBE1₁ presented the highest antidiabetic potential (79.92%±3.9) besides moderate phytochemical and antioxidant activity. Highest phenolic content (75.85±3.6 μgGAE/mg), flavonoid content (45.39±2μgQE/mg), antioxidant capacity (99.9±3.7 μgAAE/mg), reducing power (94.82±3.9 μgAAE/mg) and radical scavenging activity (DPPH; 86.28±3%, ABTS; 98.9±2.3%, MC; 59.01±2.7%) was reported by MbBE1₆ making it a potential source of antioxidant agents. Column chromatography of MbBE1₁ produced two refractions (MbBE2₁ and MbBE2₂) which reported moderate phytochemical and high antioxidant activity. High α-amylase and urease inhibition with low lipase inhibition was observed. MbBE2₁ depicted the largest ZOI against <em>S.enterica</em> (SE) (12±0.7 mm) and MbBE2₂ against Methicillin-resistant <em>S.aureus</em> (MRSA) (12±0.5 mm). Column chromatography of MbBE1₆ produced four refractions (MbBE3₁ – MbBE3₄) with moderate phytochemical and antioxidant activity without a single dominant fraction. Antibacterial activity remained low with the average ZOI sized at 7±0.5 mm. MbBE1₅ reported the highest anti-urease at 95.7%±4.7 and significant lipase inhibition at 85.5%±3. MbBE1₅, MbBE2₂ MbBE3₂ resulted in crystal formation later characterized as Lupeol with moderate phytochemical and antioxidant activity.</p></div><div><h3>Conclusion</h3><p>The study concludes that <em>M.buxifolia</em> is a potential source of bioactive components and can be further explored through modern strategies.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140180016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective benefits of ethyl alcohol extract of Piper betel L. to prevent colon carcinogenesis","authors":"V. Radhalakshmi,&nbsp;Maya Raman,&nbsp;Minnu Rose Joy","doi":"10.1016/j.prenap.2024.100024","DOIUrl":"10.1016/j.prenap.2024.100024","url":null,"abstract":"<div><p><em>Piper betel L</em>. is a traditional nutritional and medicinal food, known for its rich phytochemical components. In the current study, the role of the ethyl alcohol extract of betel leaf (<em>Piper betel</em>) against human colorectal carcinoma (HT29 cells), was investigated. The extract consists of phenolic compounds and flavonoids that are potent antioxidants and antimicrobial agents. Exposure of human colon carcinoma cells, to the 80% ethyl alcohol extract resulted in apoptotic cell death, fragmentation, downregulation in the expression levels of Bcl-XL, and upregulation of p53. The results indicated the inhibition of cell proliferation in a dose-dependent manner. The results highlight the activation of the mitochondrial apoptotic pathway in response to the treatment with ethanolic extracts of betel leaves. The treatment induced DNA damage leading to G1 cell cycle arrest and apoptosis. Low cytotoxicity and anticarcinogenic effects of the ethyl alcohol extracts of betel leaf on HT29 suggest an alternative approach to human colon cancer therapy (combination therapy) and highlights its significance as a functional food or food supplement.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100024"},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Propolis of Tubuna bee (Scaptotrigona bipunctata) from the Meliponini tribe alleviates glycaemic, oxidative, and inflammatory conditions during STZ induced diabetes in Wistar rats also aiding in injury recovery","authors":"Henrique Mautone Gomes ,&nbsp;Gabriel Conti Dalló ,&nbsp;Alberto Martins Silva ,&nbsp;Alexandre Kleber Silveira ,&nbsp;Jaison Ferreira dos Santos ,&nbsp;Lucas dos Santos da Silva ,&nbsp;Ingrid Vicente Farias ,&nbsp;Wania Aparecida Partata ,&nbsp;Flávio Reginatto ,&nbsp;Daniel Pens Gelain ,&nbsp;José Cláudio Fonseca Moreira","doi":"10.1016/j.prenap.2024.100025","DOIUrl":"10.1016/j.prenap.2024.100025","url":null,"abstract":"<div><p>This study was designed to characterize and describe the <em>in vivo</em> effects of an ethanolic propolis extract (EHAP) from the native Brazilian bee <em>Tubuna (Scaptotrigona bipunctata).</em> Propolis has been recently proposed as an adjuvant during diabetes, mainly because propolis possesses various natural compounds without the elevated sugar quantities found in pure honey. Compounds with biological activity and <em>in vitro</em> antioxidant potential have been detected in propolis from <em>Tubuna</em>. Streptozotocin (STZ) is a well-documented model to induce experimental diabetes in rodents. We have detected potential compounds in <em>Tubuna</em> propolis and observed the effects of daily oral treatment with EHAP in Wistar rats during experimental diabetes. EHAP improved glucose tolerance in STZ animals without significantly changing net and organ weight. STZ increases levels of TBARS and Carbonyl, and reduces levels of -SH, effects partially controlled by EHAP, particularly in serum. EHAP decreases inflammatory mediators TnF-ɑ and IL-1β in diabetic animals and decreases paw swelling during carrageenan induced edema. In conclusion, <em>Tubuna</em> propolis constituents possess interesting biological activities to aid in several diseases, but this still needs further studies in these particular pathologies. This is the first work to characterize <em>Tubuna</em> propolis from Rio Grande do Sul and the first work to describe its effects in an animal model of experimental diabetes or any animal model of disease. Treatment with EHAP shows potential to regulate conditions during STZ induced diabetes, aiding in injury recovery paw edema. Mechanistic studies, other models of long-term diabetes or metabolic syndrome, and a complete characterization of propolis from <em>Tubuna</em> are potential targets for future studies.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}