复方Febrojith对实验性动物模型中CD4+/CD8+ T细胞及相关炎症因子的免疫调节作用

Svenia P. Jose , S. Sheethal , M. Ratheesh , A. Sindhu , S. Sandya , Aditya Asish , Sony Rajan
{"title":"复方Febrojith对实验性动物模型中CD4+/CD8+ T细胞及相关炎症因子的免疫调节作用","authors":"Svenia P. Jose ,&nbsp;S. Sheethal ,&nbsp;M. Ratheesh ,&nbsp;A. Sindhu ,&nbsp;S. Sandya ,&nbsp;Aditya Asish ,&nbsp;Sony Rajan","doi":"10.1016/j.prenap.2025.100211","DOIUrl":null,"url":null,"abstract":"<div><div>Herbal medicines have been in the mainstay of alternative system of medicine, effectively employed for thousands of years to modulate immune system functions. In the present study, we aimed to investigate the immunomodulatory action of a polyherbal formulation febrojith (FB), on cyclophosphamide-induced immunosuppressed rats. For this study, rats were treated with 10 mg/kg of cyclophosphamide (Cy) orally for 30 days. Levamisole (Std) was used as the standard drug. The animals were divided into four groups: Group 1: Normal control (NC); Group 2: Cy treated; Group 3: Cy + Std (50 mg/kg bwt); Group 4: Cy + FB (100 mg/ kg bwt). The experimental duration was 30 days. The immunomodulatory effects of FB were evaluated by analyzing various parameters associated with humoral and cell-mediated immune responses. The results showed that the FB treatment in immunosuppressed rats alleviated significant changes in organ index and haematological markers. Furthermore, FB treatment significantly balanced Th1 type cytokines (IFN-γ and IL-2) and Th2 type cytokines (IL-4 and IL-10) levels and thereby regulating and restoring immune functions. FB also enhanced the humoral immune response. Additionally, FB normalized antioxidant enzymes levels compared to the Cy treated group, thereby protecting the normal functioning and architecture of spleen and thymus. Flow cytometry analysis of T-cells subsets (CD 3+, CD4+ and CD 8+) revealed that FB treated group significantly improved the progression of immune cells compared to the Cy- treated group. Histopathological analysis further confirmed that FB treatment reversed Cy induced pathological alterations and protected immune organs. These findings demonstrate that FB can serve as an effective phytotherapeutic agent with potent immunomodulatory action.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100211"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunomodulatory effect of a polyherbal formulation (Febrojith) on CD4+/CD8+ T cells and associated inflammatory cytokines in experimental animal model\",\"authors\":\"Svenia P. Jose ,&nbsp;S. Sheethal ,&nbsp;M. Ratheesh ,&nbsp;A. Sindhu ,&nbsp;S. Sandya ,&nbsp;Aditya Asish ,&nbsp;Sony Rajan\",\"doi\":\"10.1016/j.prenap.2025.100211\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Herbal medicines have been in the mainstay of alternative system of medicine, effectively employed for thousands of years to modulate immune system functions. In the present study, we aimed to investigate the immunomodulatory action of a polyherbal formulation febrojith (FB), on cyclophosphamide-induced immunosuppressed rats. For this study, rats were treated with 10 mg/kg of cyclophosphamide (Cy) orally for 30 days. Levamisole (Std) was used as the standard drug. The animals were divided into four groups: Group 1: Normal control (NC); Group 2: Cy treated; Group 3: Cy + Std (50 mg/kg bwt); Group 4: Cy + FB (100 mg/ kg bwt). The experimental duration was 30 days. The immunomodulatory effects of FB were evaluated by analyzing various parameters associated with humoral and cell-mediated immune responses. The results showed that the FB treatment in immunosuppressed rats alleviated significant changes in organ index and haematological markers. Furthermore, FB treatment significantly balanced Th1 type cytokines (IFN-γ and IL-2) and Th2 type cytokines (IL-4 and IL-10) levels and thereby regulating and restoring immune functions. FB also enhanced the humoral immune response. Additionally, FB normalized antioxidant enzymes levels compared to the Cy treated group, thereby protecting the normal functioning and architecture of spleen and thymus. Flow cytometry analysis of T-cells subsets (CD 3+, CD4+ and CD 8+) revealed that FB treated group significantly improved the progression of immune cells compared to the Cy- treated group. Histopathological analysis further confirmed that FB treatment reversed Cy induced pathological alterations and protected immune organs. These findings demonstrate that FB can serve as an effective phytotherapeutic agent with potent immunomodulatory action.</div></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"7 \",\"pages\":\"Article 100211\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199725000710\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725000710","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

草药一直是替代医学系统的中流砥柱,数千年来有效地用于调节免疫系统功能。在本研究中,我们旨在研究复方febrojith (FB)对环磷酰胺诱导的免疫抑制大鼠的免疫调节作用。在本研究中,大鼠口服10 mg/kg环磷酰胺(Cy) 30天。左旋咪唑(Std)作为标准药物。将实验动物分为四组:第一组:正常对照组;第二组:Cy治疗;第三组:Cy + Std(50 mg/kg bwt);第4组:Cy + FB(100 mg/ kg bwt)。试验期30 d。通过分析与体液和细胞介导的免疫反应相关的各种参数来评估FB的免疫调节作用。结果表明,免疫抑制大鼠经FB治疗后,其脏器指数和血液学指标均有明显改善。此外,FB处理显著平衡Th1型细胞因子(IFN-γ和IL-2)和Th2型细胞因子(IL-4和IL-10)水平,从而调节和恢复免疫功能。FB还能增强体液免疫反应。此外,与Cy处理组相比,FB使抗氧化酶水平正常化,从而保护脾脏和胸腺的正常功能和结构。t细胞亚群(cd3 +、CD4+和cd8 +)的流式细胞术分析显示,与Cy治疗组相比,FB治疗组显著改善了免疫细胞的进展。组织病理学分析进一步证实,FB治疗可逆转Cy诱导的病理改变,保护免疫器官。这些结果表明,白藜芦醇可以作为一种有效的植物治疗剂,具有强大的免疫调节作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunomodulatory effect of a polyherbal formulation (Febrojith) on CD4+/CD8+ T cells and associated inflammatory cytokines in experimental animal model
Herbal medicines have been in the mainstay of alternative system of medicine, effectively employed for thousands of years to modulate immune system functions. In the present study, we aimed to investigate the immunomodulatory action of a polyherbal formulation febrojith (FB), on cyclophosphamide-induced immunosuppressed rats. For this study, rats were treated with 10 mg/kg of cyclophosphamide (Cy) orally for 30 days. Levamisole (Std) was used as the standard drug. The animals were divided into four groups: Group 1: Normal control (NC); Group 2: Cy treated; Group 3: Cy + Std (50 mg/kg bwt); Group 4: Cy + FB (100 mg/ kg bwt). The experimental duration was 30 days. The immunomodulatory effects of FB were evaluated by analyzing various parameters associated with humoral and cell-mediated immune responses. The results showed that the FB treatment in immunosuppressed rats alleviated significant changes in organ index and haematological markers. Furthermore, FB treatment significantly balanced Th1 type cytokines (IFN-γ and IL-2) and Th2 type cytokines (IL-4 and IL-10) levels and thereby regulating and restoring immune functions. FB also enhanced the humoral immune response. Additionally, FB normalized antioxidant enzymes levels compared to the Cy treated group, thereby protecting the normal functioning and architecture of spleen and thymus. Flow cytometry analysis of T-cells subsets (CD 3+, CD4+ and CD 8+) revealed that FB treated group significantly improved the progression of immune cells compared to the Cy- treated group. Histopathological analysis further confirmed that FB treatment reversed Cy induced pathological alterations and protected immune organs. These findings demonstrate that FB can serve as an effective phytotherapeutic agent with potent immunomodulatory action.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信