Journal of Sustainable Agriculture and Environment最新文献

{"title":"Extracts From Perilla frutescens and Artemisia argyi Offer a Novel Strategy for Sustainable Integrated Management of Citrus Huanglongbing","authors":"Libo Wu,&nbsp;Weiping Zhu,&nbsp;Shaolong Zhang,&nbsp;Hang Chen,&nbsp;Yu Shi,&nbsp;Chunxi Zhao,&nbsp;Gecheng Ouyang,&nbsp;Hao Liu,&nbsp;Xiaolin Xie,&nbsp;Wenli Chen","doi":"10.1002/sae2.70192","DOIUrl":"https://doi.org/10.1002/sae2.70192","url":null,"abstract":"<p>Citrus Huanglongbing (HLB), a devastating global citrus disease caused by the unculturable phloem-limited bacterium <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las), poses a significant threat to citrus production. Addressing the challenges associated with the in vitro culture of <i>C</i>Las and the limitations of current chemical control strategies, such as drug resistance and environmental pollution, this study investigated the potential of traditional Chinese medicinal extracts. In vitro antimicrobial assays revealed that extracts from <i>Perilla frutescens</i> (<i>P. frutescens)</i>, <i>Artemisia argyi</i> (<i>A. argyi</i>), and their combination exhibited concentration-dependent growth inhibition against two <i>C</i>Las close lineage bacteria like <i>Agrobacterium tumefaciens</i> GV3101 (GV3101) and <i>Sinorhizobium meliloti</i> 1201 (S.M), as well as antimicrobial activity against plant pathogens like <i>Pseudomonas syringae</i> pv. <i>Maculicola</i> (<i>Psm</i>) ES4326/<i>AvrRpt2</i> and <i>Pseudomonas syringae</i> pv<i>. Tomato</i> (<i>Pst</i>) DC3000/<i>AvrRps4</i>. These extracts significantly reduced <i>C</i>Las proliferation in leaves of <i>Catharanthus roseus</i> (periwinkle)and <i>Citrus</i>. RT-qPCR analysis indicated a significant upregulation (three- to fivefold increase) of key marker genes <i>NPR1</i>(non‑expressor of PR genes 1, a master regulator of SAR), <i>PR1</i>(pathogenesis‑related 1, a marker of SAR) of systemic acquired resistance (SAR) and <i>RBOHD</i>(respiratory burst oxidase homolog D, a ROS‑producing enzyme in PTI) of pathogen-associated molecular pattern-triggered immunity (PTI) pathways. Notably, these extracts exhibited a repellency rate exceeding 90% against adults of the <i>Diaphorina citri</i> (Asian citrus psyllid). Field treatment further confirmed its efficacy in reducing <i>C</i>Las. This study is the first to systematically reveal triple functions of <i>P. frutescens</i>—<i>A. argyi</i> extract, encompassing “antibacterial activity, immune activation, and vector control,” offering a novel strategy for the sustainable integrated management of HLB, this eco-friendly alternative exhibits significant potential for the biocontrol of plant diseases and insect pests.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding Cities at the Fringe: The Role and Value of Peri-Urban Agriculture in Western Sydney, Australia","authors":"Ian Sinclair,&nbsp;Basant Maheshwari,&nbsp;Jason K. Reynolds","doi":"10.1002/sae2.70210","DOIUrl":"https://doi.org/10.1002/sae2.70210","url":null,"abstract":"<p>Producing food in the face of global population growth and urbanisation has become a critical challenge. Peri-urban areas, the transitional spaces on the outskirts of cities, play a crucial role in agricultural food production, yet they are subject to rapid changes due to urban encroachment. This article examines the peri-urban conflict in Western Sydney, Australia, where agricultural lands are consumed by urban and rural residential development. Using data from the Australian Bureau of Statistics (ABS), the study analyses the contribution of peri-urban areas to vegetable production. The results reveal that the Sydney Peri-Urban Area is the primary region for perishable vegetable production in New South Wales, contributing 46% of the total production value. This study is the first to combine detailed ABS data sets to demonstrate both the scale and value of peri-urban food production in Sydney. It highlights the significance of peri-urban areas in supplying fresh produce close to the market and population centres. It emphasises the need to preserve agricultural land to ensure food security. The changing food production scenarios in peri-urban areas demand continuous spatial reconfiguration, making the protection of these lands a complex challenge. Effective preservation strategies require addressing land use conflicts, negative public perceptions, economic factors and environmental concerns. This research highlights the significance of peri-urban agriculture in maintaining food supply and advocates for informed urban planning policies that protect agricultural lands during urban expansion.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149145638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Root Inducing Biostimulants on Vegetative Recovery, Establishment and Phenological Development of Globe Artichoke Under Field Conditions: Preliminary Results","authors":"Marco Cossu,&nbsp;Paola A. Deligios,&nbsp;Luigi Ledda,&nbsp;Giulia Pudda,&nbsp;Michele Saba,&nbsp;Maria Teresa Tiloca,&nbsp;Sara Melito","doi":"10.1002/sae2.70206","DOIUrl":"https://doi.org/10.1002/sae2.70206","url":null,"abstract":"<p>Globe artichoke (<i>Cynara cardunculus</i> L. var. <i>scolymus</i> (L.) Fiori) is a perennial crop widely cultivated in Mediterranean regions. Spinoso sardo, a Sardinian varietal type propagated agamically through quiescent buds (“ovoli”), is often characterized by heterogeneous growth, asynchronous phenological development, variable yields and sanitary constraints. This study evaluated the effects of two commercial root-targeted biostimulants (Radifarm® and Kendal Root®), applied to <i>ovoli</i> and/or as foliar treatments, either individually or in combination, under open-field forcing conditions. Biostimulant treatments were associated with trends in phenological development over time, with treated plants generally showing more advanced BBCH stages compared with the control, although differences were not statistically significant at <i>p</i> &lt; 0.05. In contrast, biostimulant applications significantly reduced the incidence of plants failing to resume vegetative growth, with the highest proportion of missing plants observed in the control treatment (χ2 = 14.16, df = 6, <i>p</i> = 0.028). Overall, the results suggest that root-targeted biostimulants may support early plant establishment and influence phenological progression of globe artichoke under forcing cultivation conditions. Further research is required to confirm these effects under different environments and growing conditions.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Nitrogen-Starved Wheat: A Multi-Omics Perspective from Epigenome Regulation to Grain Quality”","authors":"","doi":"10.1002/sae2.70209","DOIUrl":"https://doi.org/10.1002/sae2.70209","url":null,"abstract":"<p>Ali, B., Z. Khan, and N. Imin. 2026. “Nitrogen-Starved Wheat: A Multi-Omics Perspective From Epigenome Regulation to Grain Quality.” <i>Journal of Sustainable Agriculture and Environment</i> 5, no.3: e70193. https://doi.org/10.1002/sae2.70193.</p><p>The authors would like to draw readers' attention to the following errors.</p><p><b>1. Correspondence details</b></p><p>The corresponding author's email address was given incorrectly as “<span>[email protected]</span>”. This should have read: “<span>[email protected]</span>”.</p><p><b>3. Section 3.3</b></p><p>The first sentence of the second paragraph reads:</p><p>“Moderate N reduction to 210 kg ha⁻¹ delays flag leaf senescence”</p><p>This is incorrect and it should have been:</p><p>“Moderate N reduction to 210 kg N ha⁻¹ delays flag leaf senescence.”</p><p><b>4. Sections 3.3 and 5</b></p><p>Section 3.3 should have concluded: “The molecular basis of this yield loss across developmental stages is examined in Section 5.”</p><p><b>5. Section 4.5.3</b></p><p>In the last paragraph of this section, the second sentence reads:</p><p>“Whether analogous transgenerational epigenetic inheritance occurs in wheat remains to be demonstrated remains undemonstrated.”</p><p>This is incorrect and it should have read:</p><p>“Whether analogous transgenerational epigenetic inheritance occurs in wheat remains to be demonstrated.”</p><p>We apologize for these errors.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Soil Organic Carbon Potential in Sustainable Agriculture","authors":"Pratima Poudel,&nbsp;Rajan Ghimire","doi":"10.1002/sae2.70208","DOIUrl":"https://doi.org/10.1002/sae2.70208","url":null,"abstract":"<p>Soil organic carbon (SOC) is central to soil health, crop productivity, and agroecosystem resilience because of its influence on soil physical, chemical, and biological properties and processes. However, increasing SOC does not uniformly improve crop productivity, as SOC responses to management are often slow and the resulting agronomic benefits depend on soil, climate, and management context. In this narrative review, we synthesize evidence from arable field studies without constraining geographic boundaries to examine SOC-associated soil processes, long-term SOC changes under agricultural management, and relationships between SOC content and crop yield. Nine long-term field studies spanning diverse geographic and climatic regions were used to summarize changes in SOC over time under contrasting management practices. To explore SOC-yield relationships, studies reporting both SOC and yield were compiled from long-term field studies of corn and wheat as examples. Across these studies, SOC content was positively associated with corn and wheat yields, although the strength of these relationships varied and likely reflected interactions among soil, climate, cropping systems, and management. Specifically, the slow and context-dependent nature of SOC responses, climate variability, trade-offs between C sequestration and agronomic benefits, and socio-economic barriers to implementing SOC-enhancing practices pose major challenges in adopting management strategies that increase SOC. Addressing these challenges will require long-term field monitoring, optimization of C and nutrient inputs and soil disturbance, climate-adaptive management, and supportive economic and policy frameworks. Overall, SOC management should move beyond increasing C stocks alone toward context-specific strategies that enhance SOC-associated soil functions and support crop productivity and agroecosystem resilience.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148848844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nutrient-Based Biostimulant Application Partially Alleviates Negative Effects of Glyphosate Drift on Wheat","authors":"Juqi Li,&nbsp;Peter Boutsalis,&nbsp;Zed Rengel,&nbsp;Christopher Preston","doi":"10.1002/sae2.70207","DOIUrl":"https://doi.org/10.1002/sae2.70207","url":null,"abstract":"<p>The extensive use of glyphosate for weed control in-crop poses significant risks to non-target crops, such as wheat, due to spray drift. Biostimulants are widely used to improve nutrient uptake, stimulate root growth, and enhance tolerance to abiotic stress. These growth effects of biostimulants may aid in alleviating glyphosate-induced damage in non-target crops. This study evaluated three biostimulants (Popul8, Nero and Bio-Forge) applied as foliar treatment 3 days prior to simulated glyphosate drift. Glyphosate was applied to wheat (<i>Triticum aestivum</i> L.) at Zadoks 13 growth stage at 2%, 5% and 10% v/v of the recommended rate (equivalent to 18, 45 and 90 g ae ha⁻¹ respectively). None of biostimulants alleviate glyphosate-induced growth inhibition under moderate glyphosate exposure (5% and 10% v/v of the recommended rate). However, Bio-Forge significantly enhanced wheat tolerance to low glyphosate exposure (2% v/v of the recommended rate), and shoot and root dry weight were 20% and 35% higher, respectively, than in the glyphosate-only treatment (G2). The protective effect of Bio-Forge at low glyphosate exposure may be associated with modulation of ethylene-associated pathways and enhanced root development rather than direct neutralisation of glyphosate activity. The precise physiological mechanisms underlying the protective effect of Bio-Forge require further investigation.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148848785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Iron Oxide Nanoparticles Confer Induced Pre-sowing Drought Tolerance in Glycine max via Enhanced Soil-Plant Physiological Responses","authors":"Usman Shafqat,&nbsp;Awais Maqsood,&nbsp;Farasat Haider,&nbsp;Ali Raza Kashif,&nbsp;Basit Ali Khan,&nbsp;Abdullah K. Alanazi,&nbsp;Roberto Rodriguez-Ramirez,&nbsp;Basit Ali","doi":"10.1002/sae2.70203","DOIUrl":"https://doi.org/10.1002/sae2.70203","url":null,"abstract":"<p>Drought stress severely affects soybean (<i>Glycine max</i> L.) growth and productivity by disrupting physiological and metabolic processes. This study evaluated the potential of biogenic iron oxide nanoparticles green synthesised using <i>Pistacia khinjuk</i> L. leaf extract to improve soybean tolerance to water deficit. The iron oxide nanoparticles were characterised using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier-transform infrared spectroscopy and X-ray diffraction. The nanoparticles were applied to soil at concentrations of 0, 25, 50, 75 and 100 ppm under well-watered conditions at 100% pot capacity and drought-stressed conditions at 70% pot capacity. Drought stress reduced germination by 22.6%, plant growth by 18.7%–25.0%, and chlorophyll content by 20.5%. Meanwhile, soil application of iron oxide nanoparticles, particularly at 75–100 ppm, improved germination, plant growth and photosynthetic pigment content. Nanoparticle treatments also enhanced enzymatic and non-enzymatic antioxidant defences and reduced malondialdehyde accumulation, hydrogen peroxide content and electrolyte leakage by up to 49.8%, 43.2%, and 46.7%, respectively. Net photosynthesis, stomatal conductance, relative water content, plant iron accumulation, soil microbial biomass carbon and dehydrogenase activity were also improved. Multivariate analyses indicated strong positive relationships among antioxidant activity, water status, photosynthetic performance and plant growth, whereas oxidative damage indicators were negatively associated with these traits. Overall, soil-applied biogenic iron oxide nanoparticles improved soybean physiological resilience under controlled drought conditions. However, field-scale validation and long-term environmental assessment are required before practical agricultural application.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature-Based Solutions for Climate-Smart Agriculture: Current Status and Perspectives","authors":"Jilliane Clare Lu,&nbsp;Sagun Mahajan,&nbsp;Cheng-Chun He,&nbsp;Shu-Yuan Pan","doi":"10.1002/sae2.70205","DOIUrl":"https://doi.org/10.1002/sae2.70205","url":null,"abstract":"<p>Nature-based solutions (NbS) have emerged as a promising strategy for agricultural systems to mitigate and adapt to climate change. These include practices such as agroforestry, organic amendments, and conservation agriculture, which can provide several ecosystem services and benefits including enhanced soil health, carbon sequestration, and increased biodiversity. Despite their growing prominence, robust evidence on the overall performance of NbS remains limited, particularly regarding their co-benefits, trade-offs, and environmental impacts. This study presents global case studies that highlight the advantages and limitations of various NbS practices, as well as current challenges to their widespread implementation. We explore opportunities for integrating NbS into the transition from linear, intensive farming towards circular agriculture, unfolding multiple benefits across environmental, economic, and social dimensions. We evaluate the role of life cycle assessment (LCA) as a comprehensive tool for quantifying environmental impacts and supporting evidence-based decision-making. Drawing on these insights, we conceptualise a framework that integrates NbS and circularity principles into agricultural systems, with LCA as the quantitative backbone complemented by other economic and social assessments. Nevertheless, further research is still necessary to improve conventional LCA so that it can capture the multifunctionality and diverse ecosystem services provided by NbS. Advancing these methods will aid in the development of a robust framework for implementing, assessing, and monitoring NbS in agricultural systems, supporting the creation of policies that promote climate-smart agriculture.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using P-Mobilization Traits in Modern Maize Varieties for Optimizing the P Fertiliser Use Efficiency of Struvite Under P Limitation: First Evidence From a Pot and Field Trial","authors":"Michelle Natalie Herrmann,&nbsp;Daniel Wanke,&nbsp;Peteh Mehdi Nkebiwe,&nbsp;Torsten Müller","doi":"10.1002/sae2.70202","DOIUrl":"https://doi.org/10.1002/sae2.70202","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Current recycling measures for closing the phosphorus (P) cycle are limited by the lower fertiliser use efficiencies of recycled products. Increasing P use efficiency (PUE) through maize genotype selection and management may offer a sustainable solution, but the potential of variety choice and its interaction with placed fertilisation for enhancing recycled fertilisers' efficiency remains unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Four maize genotypes differing in P efficiency were tested in a pot trial, where microbial P, phosphatase activity, and Olsen-P were measured and their links to PUE were investigated. Three of these genotypes were examined in a two year field trial comparing two fertiliser types (diammonium phosphate (DAP); struvite) and application methods (broadcast vs. placed).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>Stabil</i> showed higher microbial biomass P and <i>LG_30258</i> higher phosphatase activity than other varieties. Although not significant, the PUE, expressed as biomass divided by fertilisation, varied among genotypes (<i>Amaroc</i> 3.4 x higher than <i>Ricardinio</i>). In the field, no genotype × management interaction was detected and genotypic differences became minimal (<i>LG_30258</i> 1.4 x higher than <i>Stabil</i>). Struvite performed comparably to DAP across both years, while fertiliser placement only increased biomass and P offtake in the drier year, 2022. P mobilisation traits could not predict PUE, however, P offtake in the pot and field trial followed the same ranking order as the P mobilization traits.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study highlights that the slow-release fertiliser, struvite, has a comparable fertiliser efficiency than DAP, and fertiliser placement can further enhance PUE in dry seasons, independent of the fertiliser source. Although slight genotypic variance in P mobilisation exists in modern maize varieties and can predict P responsiveness, the influence of genotype choice and management options appeared additive rather than synergistic since genotype x management interaction was not significant. Future research should further explore how to exploit synergies between genotypes, management, and fertilisers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compartment-Specific Functional and Taxonomic Responses of Plant Growth-Promoting Microbiomes Following Oxytetracycline Trunk Injection in Citrus","authors":"Antonio Castellano-Hinojosa,&nbsp;Jesús González-López,&nbsp;Sarah L. Strauss,&nbsp;Ute Albrecht","doi":"10.1002/sae2.70200","DOIUrl":"https://doi.org/10.1002/sae2.70200","url":null,"abstract":"<p>Huanglongbing (HLB), caused by <i>Candidatus</i> Liberibacter asiaticus, is the most destructive disease affecting citrus production worldwide. Trunk injection of oxytetracycline (OTC) is increasingly used to suppress the pathogen and improve tree performance, yet its ecological consequences for plant-associated microbiomes remain poorly understood. Here, we evaluated the compartment-specific responses of plant growth–promoting microbiomes to systemic OTC treatment in HLB-affected citrus trees under commercial orchard conditions. Using shotgun metagenomics combined with targeted detection of plant growth–promoting genes, we analyzed functional and taxonomic dynamics across leaves, bark, roots, and rhizosphere at three time points (3 days, 3 weeks, and 3 months) following treatment. OTC injection reduced pathogen abundance in leaves and improved yield, fruit weight, and juice quality. Aggregated plant growth–promoting functional profiles remained largely stable across compartments. However, taxonomic shifts were nonetheless detected within microbial lineages carrying these functions, suggesting that comparable functional potential may be maintained despite changes in the microbial lineages encoding it. Bark communities showed transient enrichment of nutrient acquisition and interaction-related functions, while the rhizosphere exhibited taxonomic turnover without consistent functional shifts. In contrast, root microbiomes displayed temporally structured reorganization of interaction-associated functions and were the only compartment where functional composition correlated with plant performance. Genome-resolved analyses further suggested early enrichment of stress-tolerant and metabolically versatile taxa following treatment. We conclude that systemic OTC trunk injection in citrus was associated with taxonomic redistribution rather than broad functional loss of plant-associated microbiomes, with functionally relevant consequences emerging selectively in the root microbiome. These findings highlight the importance of spatially resolved microbiome analyses for understanding the ecological impacts of antibiotic use in perennial crop systems and for evaluating the sustainability of disease-management strategies.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148754050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}