Biotechnology Notes最新文献

{"title":"Cell bank system, establishment, and application in the virus research, diagnosis, and biopharmaceutical industries","authors":"Sina Soleimani ,&nbsp;Mohammadreza Ghorani","doi":"10.1016/j.biotno.2025.08.001","DOIUrl":"10.1016/j.biotno.2025.08.001","url":null,"abstract":"<div><h3>Background</h3><div>The use of cells in research and diagnostic studies is important. This is of paramount importance in the biopharmaceutical product industry because cells are one of the most important platforms in the biopharmaceutical industry.</div></div><div><h3>Objective</h3><div>The availability of highly efficient and suitable cells that can be continuously used for the mass production of biotechnological products is crucial for maintaining human health and hygiene. So, the cells used in this comprehensive system were studied, evaluated, documented, and stored for continuous and efficient use.</div></div><div><h3>Study design</h3><div>This review article discusses all aspects of cells involved in establishing this system as a Cell Bank System (CBS). Cell banking facilities, specialized laboratories, appropriate equipment, cold rooms, cryopreservation instruments, and cell authentication and characterization are mentioned. Cell handling, row materials tests, documentation, intellectual property registration, storage, backup, and transportation were explained. In addition, policies for cell retesting and revival are discussed.</div></div><div><h3>Result</h3><div>Based on the roadmap designed in this study, a comprehensive cell bank system can be formed for using in cell preparation, propagation, storage, and use of cells.</div></div><div><h3>Discussion</h3><div>Considering that in the pharmaceutical industry, there is a need for a reliable, permanent, and uniform source of cells to produce and quality control the product, which can have significant effects on the products, by using this system and performing all of its aspects, diagnostic centers, researchers, and pharmaceutical manufacturers can access a reliable and persistent source of culture for related operations.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 209-221"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Micro-biome engineering in enhancing Food safety and quality","authors":"Anand Kumar ,&nbsp;Abhishek Bisht ,&nbsp;SammraMaqsood ,&nbsp;SaiqaAmjad ,&nbsp;Sapna baghel ,&nbsp;Swapnil Ganesh Jaiswal ,&nbsp;Shuai wei","doi":"10.1016/j.biotno.2025.01.001","DOIUrl":"10.1016/j.biotno.2025.01.001","url":null,"abstract":"<div><div>Microbiome engineering has emerged as a transformative approach to enhancing food safety and quality by strategically modulating microbial communities. This review critically examines state-of-the-art techniques, including synthetic biology, artificial intelligence (AI), and systems biology, that are revolutionizing our ability to improve nutritional profiles, extend shelf life, and optimize food production processes. The review further explores complex social, ethical, and regulatory considerations, emphasizing the importance of robust public engagement and the establishment of standardized frameworks to ensure safe and effective implementation. While microbiome engineering holds significant promise for revolutionizing food safety and quality control, further research is needed to address critical challenges, including understanding microbial dynamics in complex food systems and developing harmonized regulatory frameworks. By bridging interdisciplinary gaps, this paper underscores the necessity of collaborative efforts to unlock the full potential of microbiome-driven innovations for a more resilient and sustainable food industry.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 67-78"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143132795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supercritical CO2 extraction of artemisinin from Artemisia annua plant and the biotechnological production of artemisinin precursors: A dual-focus review","authors":"Babatunde Oladipo,&nbsp;Tunde V. Ojumu","doi":"10.1016/j.biotno.2025.05.003","DOIUrl":"10.1016/j.biotno.2025.05.003","url":null,"abstract":"<div><div>Artemisinin, a vital compound renowned for its potent antimalarial properties, has garnered significant attention due to its therapeutic importance and critical role in combating malaria. The extraction process is essential in recovering artemisinin from <em>Artemisia annua</em> L. plant. Supercritical carbon dioxide (scCO₂) extraction has emerged as a highly effective and eco-friendly technique, offering improved efficiency, selectivity, and greener processing than conventional solvent-based methods. Despite this advancement, plant-derived artemisinin faces challenges in meeting global demand due to naturally low yields, seasonal variation, and agricultural limitations. Biotechnological advances have enabled the microbial production of artemisinin precursors, such as artemisinic acid and amorphadiene, which can be chemically or enzymatically converted into artemisinin, providing a scalable and sustainable production route. Despite the significance of both approaches, existing literature often treats them in isolation. Therefore, this work provides a comprehensive review, integrating scCO₂ extraction technologies with microbial-based fermentation strategies for producing artemisinin and its precursors. Key parameters influencing scCO₂ extraction efficiency, such as CO₂ flow rate, temperature, co-solvent use, and pressure, are analyzed alongside fermentation bioprocess factors such as strain selection, pH, dissolved oxygen levels, carbon sources, and fermentation modes. By evaluating these complementary strategies, this review provides a holistic perspective aimed at improving artemisinin production yield, for accessibility and sustainability, ensuring a reliable global supply. It concludes by highlighting current challenges and proposing future directions necessary for optimizing the integrated production pipeline of artemisinin and its precursors.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 143-156"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly synthesis and optimization of CuNPs using a non-pathogenic bioflocculant from Kytococcus sedentarius","authors":"Minenhle PD. Sibisi ,&nbsp;Albertus K. Basson ,&nbsp;Zuzingcebo G. Ntombela ,&nbsp;Rajasekhar V.S.R. Pullabhotla","doi":"10.1016/j.biotno.2025.02.002","DOIUrl":"10.1016/j.biotno.2025.02.002","url":null,"abstract":"<div><div>Nanotechnology is being used to solve a variety of environmental issues, including wastewater treatment. In the present study, a rapid eco-friendly method was applied to biosynthesize and optimize copper nanoparticles (CuNPs) from <em>Kytococcus sedentarius</em>. The CuNPs characteristics were identified using X-ray diffractometer (XRD), scanning electron microscope (SEM), Fourier Transform infrared (FT-IR), Transmission electron microscope (TEM), Thermogravimetric analysis (TGA) and UV–Vis spectroscope (UV–Vis). To determine the maximum metabolic yield, the optimum dosage size, pH, temperature, salinity and cations were evaluated. The antibacterial activity of the samples against Gram-negative and Gram-positive isolates was assessed using the Kirby-Bauer Disk Diffusion Test. 28.3 nm was the average crystallite size of CuNPs revealed through XRD analysis. The SEM and TEM analysis depicted the CuNPs to be agglomerated in various sizes and forms. Elements such as Carbon (25.23 % wt), Cu (23.37 % Wt) and Oxygen (20.13 % Wt) were found in CuNPs. The nanoparticles had functional groups and a Cu–O bond at 559 cm ⁻¹. The CuNPs retained 70 % of its weight whereas the bioflocculant retained only 50 % when heated at a range of 100 °C–900 °C. The samples exhibited a UV–Vis spectra between 250 and 300 nm, at a range of 200–1400 nm. The flocculating effeciency of CuNPs was optimal at 0.2 mg/mL (92 %) and cation independent (92 %). pH 7 was the peak maximum as 98 % of the flocculating activity was obtained. The CuNPs were thermally stable than the bioflocculant as over 80 % of its flocculating activity was retained even at high temperatures (121 °C). The CuNPs were not affected by the increase in NaCl concentration with the highest NaCl concentration (35 g/L) having the highest flocculating activity of 90 %. CuNPs exhibited antimicrobial activity against both bacterial strains, with greater susceptibility observed in <em>S. aureus</em> as compared to the bioflocculant. Thus, CuNPs have a potential to be applied in wastewater treatment to replace traditional flocculants.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 89-99"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Straightforward MALDI-TOF MS based screening approach for selection of recombinant protein-expressing E. coli","authors":"I.N. Kravtsov ,&nbsp;A.I. Solovyev ,&nbsp;E.A. Potemkina ,&nbsp;A.V. Kartashova ,&nbsp;M.A. Dmitrieva ,&nbsp;K.V. Danilova ,&nbsp;I.L. Tutykhina ,&nbsp;N.B. Polyakov ,&nbsp;V.D. Desinov ,&nbsp;D.A. Egorova ,&nbsp;A.L. Gintsburg","doi":"10.1016/j.biotno.2025.02.004","DOIUrl":"10.1016/j.biotno.2025.02.004","url":null,"abstract":"<div><div>Recombinant protein production is a milestone of modern biotechnology, drug development and scientific research. When obtaining recombinant protein producers, differences in expression levels among clones necessitate screening. Traditional widely used methods include protein electrophoresis and western blot hybridization. This protocol provides high-throughput advantages by eliminating time-consuming steps inherent to traditional methods, such as cell lysis, protein extraction, purification, antibody-based detection, and gel-based analysis. MALDI-TOF MS represents a simple, rapid and cost-effective method for bacterial species identification through protein fingerprint signature in clinical diagnostics, but not practically integrated into biotechnological workflow. This study proposes a fast and easy method for screening <em>E. coli</em> clones producing recombinant proteins with MALDI-TOF MS. The proposed method demonstrated efficiency in screening of <em>E. coli</em> producing several recombinant proteins with different properties: sfGFP; bacterial DNA binding proteins IHF<em>α</em>, IHF<em>β</em>, HU; bacteriophage protein GP46 and camelid VHH antibody fragments.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 100-105"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea","authors":"Kalenahalli Yogendra,&nbsp;Harika Gadeela,&nbsp;Koppula Nithya Sree,&nbsp;Wricha Tyagi","doi":"10.1016/j.biotno.2025.02.005","DOIUrl":"10.1016/j.biotno.2025.02.005","url":null,"abstract":"<div><div>The process of generating stable transformants is time-consuming, labor-intensive, and genotype-dependent. In contrast, transient gene expression techniques, such as agroinfiltration, offer a rapid assessment of gene function and expression. Agroinfiltration, widely employed for studying gene function, has been extensively applied in leaf tissues of <em>Nicotiana benthamiana</em> and various other plant species. Despite its broad utility in various plants, to our knowledge, no prior investigation has been reported in pigeonpea. In this study, we developed an agroinfiltration method for transiently expressing a green fluorescent protein (<em>mGFP5</em>) reporter gene in four pigeonpea genotypes using syringe infiltration at the seedling stage under greenhouse conditions. The expression of the reporter gene <em>mGFP5</em> was assessed at 72-, 96-, and 120 h post-infiltration (hpi). Additionally, we assessed the effect of morphogenic genes, specifically <em>growth-regulating factor 4</em> (<em>GRF4</em>) and <em>GRF-interacting factor 1</em> (<em>GIF1</em>), from both rice and pigeonpea on the expression of <em>mGFP5</em> in four pigeonpea genotypes. Our findings demonstrate that <em>OsGRF4-GIF1</em> led to enhanced <em>mGFP5</em> expression compared to <em>CcGRF4-GIF1</em> in four diverse pigeonpea genotypes. Fluorescence could be detected till 120 hpi. Furthermore, PCR, RT-PCR, and fluorescence quantification confirmed the presence and expression of <em>mGFP5</em> at 72 hpi. Our results highlight the efficacy of agroinfiltration in quickly evaluating candidate genes in four genetically diverse pigeonpea genotypes, thereby reducing the time required for the initial assessment of constructs suitable for diverse molecular biology analyses.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 117-125"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of candidate MicroRNAs and gene targets for OSA management using in silico and in-vitro approach","authors":"Gaganjyot Kaur Bakshi ,&nbsp;Sartaj Khurana ,&nbsp;Shambhavee Srivastav ,&nbsp;Rohit Kumar ,&nbsp;Mukesh Chourasia ,&nbsp;Sudeep Bose","doi":"10.1016/j.biotno.2025.01.003","DOIUrl":"10.1016/j.biotno.2025.01.003","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) have been implicated in the pathogenesis of human diseases including sleep disorders. The aim of this study is to address the involvement of miRNAs (miR-21 and miR-29) in the pathophysiology of obstructive sleep apnea (OSA). In this study we have done integrated analysis of miRNAs with their potential gene targets as a strategy for management of OSA.</div></div><div><h3>Methods</h3><div>miRNA expression levels were quantified in healthy control group and obese vs. Non-obese OSA subjects by Quantitative real-time PCR. In-silico analysis of interplay of miRNAs with potential gene targets was done using Schrödinger Release 2023-1.</div></div><div><h3>Results</h3><div>The real time expression analysis revealed a differential expression pattern in miRNAs indicating down-regulation of miR-21 in obese OSA while miR-29 showed upregulation as compared to non-obese OSA and healthy subjects with p values of ≤0.01 and &lt;0.0001respectively. A trend was observed where target genes TGFBR2, NAMPT, and NPPB were significantly increased with p-value of ≤0.0001 and TGFBR3 and INSIG2 showed decreasing trend with p-value of ≤0.0001 between obese and non-obese OSA respectively. MD simulation analysis provided valuable information regarding the stability, flexibility, compactness and solvent exposure of the complexes over time.</div></div><div><h3>Conclusion</h3><div>miR-21 and miR-29 possesses differential expressions in obese OSA subject and exihbits strong molecular interactions with potential target genes, such as TGFBR2, NPPB, NAMPT and INSIG2. Identifying the miRNAs, genes and pathways associated with OSA can help to expand our understanding of the risk factors for the disease as well as provide new avenues for potential treatment.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 79-88"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143132794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tackling carbapenem-resistant Acinetobacter baumannii (CRAB) and their virulence factors using biosynthesized silver nanoparticles combined with imipenem","authors":"Mohamed Shawky,&nbsp;Mohamed H. Kalaba,&nbsp;Gamal M. El-Sherbiny","doi":"10.1016/j.biotno.2025.07.002","DOIUrl":"10.1016/j.biotno.2025.07.002","url":null,"abstract":"<div><div>Carbapenem-resistant <em>Acinetobacter baumannii</em> is an extremely hazardous microorganism due to its high level of resistance to a wide array of antibiotics, making it a significant public health concern. Herein, this study aimed to biofabricate silver nanoparticles using a cell-free filter derived from <em>Streptomyces</em> sp., with a focus on characterizing their physicochemical properties, and use them to combat CRAB and their virulence factors. The biofabricated Ag-NPs were predominantly spherical with an average size 50 nm, confirmed through TEM analyses, while DLS measurements showed an average hydrodynamic diameter of approximately 36.78 nm. UV–Vis spectroscopy displayed a characteristic surface plasmon resonance peak in the range of 420 nm, indicative of nanoparticle formation. XRD confirmed the crystalline structure, presenting peaks corresponding to face-centered cubic silver. FTIR spectroscopy revealed active participation of metabolite compounds derived from the <em>Streptom</em>yces cell-free filter in both reduction and stabilization processes. Eight clinical bacterial isolates were identified as CRAB using the Vitek-2 system, and biofilm formation with 100 % was assessed through Congo red and microplate assays. The MIC for Ag-NPs and imipenem (IMP) were found to be between 4 and 5 μg/mL and 13 and 15 μg/mL, respectively. Additionally, the fractional inhibitory concentration index (FICI) for the synergistic combinations of Ag-NPs and IMP ranged from 0.5 to 0.375, indicating a notable decrease in the MIC values for both IMP and Ag-NPs from 14 and 5 μg/mL to 1.75 and 1.25 μg/mL, respectively. The qRT-PCR demonstrated a significant reduction in the expression levels of the <em>Bap</em> and <em>ompA</em> genes by up to 4.0-fold (p ≤ 0.001). The time-killing assay confirmed that the bacterial strain was effectively eliminated through the synergistic action of Ag-NPs and IMP. Moreover, the cytotoxicity assessment of Ag-NPs and their combination with IMP revealed low toxicity of the combination of Ag-NPs and IMP, with an IC₅₀ of 26.13 ± 0.24 and 45.33 ± 0.21 μg/mL, respectively (p &lt; 0.0019), indicating good biosafety, while the hemolysis rates were recorded at 0.4 and 0.7 at 12 and 24 h, respectively. We concluded that the combination of Ag-NPs with IMP could serve as a promising alternative strategy for treating CRAB.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 183-195"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling and simulation of soft bio-mimetic fingers with a novel soft thumb design for bionic hand applications using ANN","authors":"Eman R.A. Soliman ,&nbsp;Ayman Nada ,&nbsp;Hiroyuki Ishii ,&nbsp;Ahmed M.R. Fathelbeb","doi":"10.1016/j.biotno.2025.05.002","DOIUrl":"10.1016/j.biotno.2025.05.002","url":null,"abstract":"<div><div>The paper presents a novel design for a soft bio-mimetic finger and soft thumb structure for bionic hand applications. It introduces an anthropomorphic pneumatic flexible finger system using a PneuNets framework to enhance flexibility and maneuverability. The research investigates the influence of geometric variations (wall thickness, chamber number, and spacing) on finger deformation, demonstrating that reduced wall thickness and augmented chambers substantially improve flexibility. A key innovation is the soft thumb design that accurately replicates the complex movements of the Carpometacarpal (CMC) joint, enabling natural opposition and dexterity. Eight models were developed for four fingers and two models for the thumb. Simulation results indicate that models with thinner walls (2 mm) achieve bending angles exceeding 80° at 120 KPa, whereas 3 mm models remain below 50°. Moreover, increasing the number of chambers enhances deformation, with each added chamber contributing approximately 41 % more flexibility. For the thumb models, we successfully mapped the motion ranges and accurately mimicked the base joint, enabling natural opposition and dexterity. Furthermore, the paper also integrates Artificial Neural Networks (ANNs) to model forward kinematics, improving the estimation of bending angles and end-tip positions, which enhances the overall adaptability and control of the system.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 164-176"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-free protein synthesis platforms for accelerating drug discovery","authors":"Filippo Caschera","doi":"10.1016/j.biotno.2025.02.001","DOIUrl":"10.1016/j.biotno.2025.02.001","url":null,"abstract":"<div><div>Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were synthesised and characterised. Off-the-shelf reagents and parallelised experimentation have enabled the exploration of many different conditions for <em>in vitro</em> protein synthesis and engineering. Herein is described how machine learning algorithms were applied for protein yield maximisation as well as for protein engineering and <em>de novo</em> design. Cell-free protein synthesis provides the biotechnological platform to unlock the power and benefit of AI/ML for drug discovery and improve human health.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"6 ","pages":"Pages 126-132"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}