Biotechnology Notes最新文献

Recombinant production and initial characterization of a novel lectin-like protein from the red alga Kappaphycus alvarezii 一种红藻凝集素样蛋白的重组制备及初步表征

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-09-30 DOI: 10.1016/j.biotno.2026.09.001

Éllen Francine Rodrigues, Gabrielly Oliveira do Couto, Lauane Ferreira de Almeida, Ana Lúcia Fachin, Renê de Oliveira Beleboni, Mozart Marins

{"title":"Recombinant production and initial characterization of a novel lectin-like protein from the red alga Kappaphycus alvarezii","authors":"Éllen Francine Rodrigues, Gabrielly Oliveira do Couto, Lauane Ferreira de Almeida, Ana Lúcia Fachin, Renê de Oliveira Beleboni, Mozart Marins","doi":"10.1016/j.biotno.2026.09.001","DOIUrl":"10.1016/j.biotno.2026.09.001","url":null,"abstract":"<div><div>Marine macroalgae are essential sources of bioactive compounds, widely explored for their pharmacological and biotechnological properties. <em>Kappaphycus alvarezii</em> (Rhodophyta), a red macroalga widely cultivated worldwide as a major source of κ-carrageenan, is emerging as a rich source of lectins with high carbohydrate-binding specificity, offering promising applications in diagnostics and antitumor therapies. This study aimed to recombinantly produce, purify, and characterize the biological activities of a novel lectin-like protein from <em>K. alvarezii</em>, designated KAL-1 lectin-like, with emphasis on its anticancer potential against human osteosarcoma U2OS cells. The recombinant KAL-1 lectin-like protein was expressed in CHO cells, purified by affinity chromatography, and subsequently evaluated for cytotoxicity and cellular selectivity using the MTT viability assay in HaCaT and U2OS cell lines. KAL-1 significantly reduced the viability of U2OS cells at very low concentrations (0.025 μM), without inducing relevant inhibitory effects on the viability of normal epithelial cells (HaCaT). These results indicate a selective antitumor action of KAL-1, suggesting its potential as a promising candidate for the development of targeted anticancer agents.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 203-210"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149140713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green-mediated gold nanoparticles from Allium cepa: Synthesis, characterization and antimicrobial properties 绿色介导的葱金纳米颗粒：合成、表征及抗菌性能。

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-02-14 DOI: 10.1016/j.biotno.2026.01.004

Aanuoluwapo Eunice Adegbola , Tesleem Olatunde Abolarinwa , Omolola Esther Fayemi

{"title":"Green-mediated gold nanoparticles from Allium cepa: Synthesis, characterization and antimicrobial properties","authors":"Aanuoluwapo Eunice Adegbola , Tesleem Olatunde Abolarinwa , Omolola Esther Fayemi","doi":"10.1016/j.biotno.2026.01.004","DOIUrl":"10.1016/j.biotno.2026.01.004","url":null,"abstract":"<div><div>Green synthesis of metal nanoparticles has gained significant interest in biomedicine due to its cost‐effectiveness and environmental compatibility. In this study, aqueous extracts of <em>Allium cepa</em> bulb and peel were employed as biogenic reducing, capping, and stabilizing agents for the synthesis of gold nanoparticles (AuNPs). The synthesized nanoparticles were tested against pathogenic bacteria. UV–Vis spectroscopy confirmed nanoparticle formation with distinct surface plasmon resonance peaks at 551 nm for bulb-derived AuNPs (OBNPs) and 539 nm for peel-derived AuNPs (OPNPs). FT-IR analysis indicated potential involvement of flavonoids, phenolic constituents, and sulphur-containing compounds in the reduction and stabilization processes, while X-ray diffraction (XRD) revealed characteristic face-centered cubic crystalline structures. SEM and TEM micrographs further showed extract-dependent morphological variations: OBNPs appeared irregular, polydisperse, and agglomerated, whereas OPNPs exhibited well-defined anisotropic features such as nanoplates and truncated plate-like structures. The broad particle size distribution (5–200 nm) reflects the influence of phytochemical composition on nucleation and growth. Energy-dispersive X-ray spectroscopy (EDX) confirmed the elemental presence of gold (>94%) in the nanoparticles. Both OBNPs and OPNPs exhibited strong antibacterial effects, with MIC and MBC values ranged from 0.625 to 1.25 and 5–10 mg/mL, respectively. Completed bacteria elimination was archived within 12–24 h. Overall, this work establishes <em>Allium cepa</em>, particularly onion peel waste, as a valuable, low-cost, and sustainable resource for stable AuNPs synthesis with potential for biomedical and antimicrobial applications.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 44-57"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147278169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico and In vitro studies of EGFR kinase and Anilide derivatives as inhibitors against Breast Cancer EGFR激酶和苯胺衍生物作为乳腺癌抑制剂的体内和体外研究

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-08-06 DOI: 10.1016/j.biotno.2026.06.002

Mala M. Parab, Pramodkumar P. Gupta, Shraddha Shetty, Santosh S. Chhajed, Geetanjali Shukla, Kshitij Bapat, Debjani Dasgupta, Prerona Boruah

{"title":"In silico and In vitro studies of EGFR kinase and Anilide derivatives as inhibitors against Breast Cancer","authors":"Mala M. Parab, Pramodkumar P. Gupta, Shraddha Shetty, Santosh S. Chhajed, Geetanjali Shukla, Kshitij Bapat, Debjani Dasgupta, Prerona Boruah","doi":"10.1016/j.biotno.2026.06.002","DOIUrl":"10.1016/j.biotno.2026.06.002","url":null,"abstract":"<div><div>The benzanilide scaffold is well known for its biological activities, such as antifungal, antibacterial, herbicide, and anti-tumor activity. Benzanilides with halogen substitution were computationally designed and screened against EGFR kinase, with erlotinib as a reference compound, using molecular docking. Two compounds were synthesized by the Schotten-Baumann reaction and confirmed based on FT-IR spectrophotometry, TLC, and sharp, narrow-range melting point analyses. The synthesized compounds were tested for antiproliferative activity and acute toxicity using the MTT assay with the (MCF-7) human breast cancer cell line. The effect of the compounds on non-cancerous cells in the human body was tested using blood lymphocytes. The mechanism of action evaluated by an in vitro cytotoxicity study revealed IC<sub>50</sub> values of compound A = 75.54 ± 4.7 μM and compound B = 178.5 ± 9.0 μM (mean ± SEM, n = 3), showing promising growth-inhibitory cytotoxic actions with increasing concentrations against EGFR in breast cancer. Docking studies revealed that synthesized compounds interacted with a similar enzyme and ATP binding site of EGFR-TK in the reference drug erlotinib, along with additional strong interactions indicating efficacy and potency. To predict better tolerability and late failure, toxicity predictions were made for the synthesized compounds. Therefore, this research aimed to demonstrate the activity of several promising benzanilide molecules.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 156-167"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148735530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics, postbiotics, and synbiotics in immune system modulation: A narrative review 免疫系统调节中的益生菌、后益生菌和合成菌：综述。

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-06-24 DOI: 10.1016/j.biotno.2026.06.001

Mina Owrang

引用次数: 0

MicroRNAs in systemic lupus erythematosus: Molecular networks, pathway dysregulation, and therapeutic targeting strategies 系统性红斑狼疮中的microrna：分子网络、通路失调和治疗靶向策略

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-09-01 DOI: 10.1016/j.biotno.2026.07.002

Mohamed M. Sadaty, Rana Mohamed, Emad El-Zayat, Salma M. Mekhemer, Amira El-Ansary, Nahla O. Mousa

{"title":"MicroRNAs in systemic lupus erythematosus: Molecular networks, pathway dysregulation, and therapeutic targeting strategies","authors":"Mohamed M. Sadaty, Rana Mohamed, Emad El-Zayat, Salma M. Mekhemer, Amira El-Ansary, Nahla O. Mousa","doi":"10.1016/j.biotno.2026.07.002","DOIUrl":"10.1016/j.biotno.2026.07.002","url":null,"abstract":"<div><div>Systemic lupus erythematosus (SLE) is a complex autoimmune disorder characterized by immune dysregulation, chronic inflammation, and multi-organ involvement. Its pathogenesis is multifactorial, involving genetic, epigenetic, and environmental factors that collectively contribute to the loss of immune tolerance. In recent years, considerable attention has focused on epigenetic mechanisms, particularly microRNAs (miRNAs), which function as post-transcriptional regulators of gene expression and play pivotal roles in immune cell differentiation and function. Emerging evidence suggests that dysregulated expressions of specific miRNAs, such as miR-146a and miR-155, may contribute to aberrant immune responses, including altered T- and B-cell activity and cytokine production in SLE. These alterations have been associated with disease activity and immunopathological processes. In addition, circulating miRNAs have been investigated as potential non-invasive biomarkers for diagnosis and disease monitoring. Advances in high-throughput technologies have facilitated the identification of miRNA expression profiles associated with SLE; however, variability between studies and limited clinical validation remain challenges. While miRNA-based therapeutic strategies are being explored, their clinical application is still under investigation.</div><div>This review highlights the role of miRNAs in SLE pathogenesis, their potential as biomarkers, and their emerging relevance as therapeutic targets, while also addressing current limitations and challenges in clinical translation.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 183-194"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of antibacterial and anti-biofilm activities of marine fungal epiphytes associated with red and green algae of the Kenyan coast 肯尼亚海岸与红藻和绿藻相关的海洋真菌附生菌的抗菌和抗生物膜活性评估。

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-06-24 DOI: 10.1016/j.biotno.2026.06.003

Aragaw Zemene Sendekie, Kimang'a Andrew Nyerere, Purity Kinya Kaaria

{"title":"Assessment of antibacterial and anti-biofilm activities of marine fungal epiphytes associated with red and green algae of the Kenyan coast","authors":"Aragaw Zemene Sendekie, Kimang'a Andrew Nyerere, Purity Kinya Kaaria","doi":"10.1016/j.biotno.2026.06.003","DOIUrl":"10.1016/j.biotno.2026.06.003","url":null,"abstract":"<div><div>Marine-derived fungal epiphytes represent an important yet largely unexplored source of bioactive compounds. This study investigates the antibacterial and anti-biofilm properties of cultivable fungal epiphytes associated with red and green algae collected from a single coastal site in Kenya. A total of 330 fungal isolates were initially identified based on their morphological characteristics. Following agar-plug screening, nine active isolates have been identified through ITS-rDNA sequencing. Ethyl acetate and methanolic fungal extracts were evaluated against six multidrug-resistant microorganisms using disc diffusion, MIC, MBC, and microtiter biofilm disruption assays. The five best-performing extracts were subjected to SEM imaging, with two (Cer sp-2 and Ulr-1) further analyzed via GC-MS. Extracts from both solvents showed remarkable antibacterial effects, producing zones of inhibition that spanned between 10.00 ± 0.00 and 29.00 ± 0.00 mm. Furthermore, MIC and MBC assays revealed strong activity, with the lowest recorded values being 0.039 mg/mL and 0.156 mg/mL, respectively. Scanning electron microscopy (SEM) analysis displayed structural changes in bacterial cells, supporting a membrane-targeting mechanism of action. They also exhibited significant anti-biofilm properties (P < 0.05-0.0001) compared to the PBS-treated control. Although most biofilm reduction percentages were relatively low, the extracts displayed measurable activity in disrupting pre-formed biofilms. GC-MS identified a diverse profile of bioactive metabolites in the two representative extracts. Overall, Kenyan coastal algae harbor bioactive fungal epiphytes with promising potential to combat multidrug-resistant and biofilm-forming pathogens. Comprehensive studies are needed to discover novel therapeutic candidates in the future.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 120-141"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenic synthesis of zinc oxide nanoparticles using Pichia cactophila-derived β-glucan as a novel capping agent: Characterization, antioxidant, antibacterial, and cytotoxicity evaluation 用毕赤酵母衍生β-葡聚糖作为新型封盖剂合成氧化锌纳米粒子：表征、抗氧化、抗菌和细胞毒性评价

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-07-17 DOI: 10.1016/j.biotno.2026.06.004

Koushik Bandlu Raghuram, Seema J. Patel, Jayalakshmi Kabbur Rajashekar, Ganesh Gopal Tilve

{"title":"Biogenic synthesis of zinc oxide nanoparticles using Pichia cactophila-derived β-glucan as a novel capping agent: Characterization, antioxidant, antibacterial, and cytotoxicity evaluation","authors":"Koushik Bandlu Raghuram, Seema J. Patel, Jayalakshmi Kabbur Rajashekar, Ganesh Gopal Tilve","doi":"10.1016/j.biotno.2026.06.004","DOIUrl":"10.1016/j.biotno.2026.06.004","url":null,"abstract":"<div><div>The present study reports the isolation and molecular identification of <em>Pichia cactophila</em> JP strain from Jogimatti Forest soil, Karnataka, India, and the first-ever use of its extracted β-glucan as a biogenic capping agent for ZnO nanoparticle synthesis. β-glucan was isolated via acid-alkali extraction (yield: 4.6% w/w dry cell weight) and employed in a co-precipitation method to synthesize YGZnNP-1. Physicochemical characterization confirmed a crystalline hexagonal wurtzite ZnO structure (JCPDS No. 36-1451), an average crystallite size of ∼28.5 nm, and a UV absorption peak at 372 nm. FTIR spectroscopy verified effective β-glucan surface functionalization. YGZnNP-1 exhibited potent dose-dependent antioxidant activity (DPPH IC<sub>50</sub>: 79.73 μg/mL), significant antibacterial activity against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em>, and favourable biocompatibility against NIH 3T3 fibroblast cells (IC<sub>50</sub>: 98.20 μg/mL). These findings establish YGZnNP-1 as a promising multifunctional nanomaterial for biomedical applications.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 142-155"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148523847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface plasmon resonance biosensors: Advancements, applications, and future directions in molecular detection 表面等离子体共振生物传感器：分子检测的进展、应用和未来方向。

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-02-13 DOI: 10.1016/j.biotno.2026.01.002

Nidal El Biyari

{"title":"Surface plasmon resonance biosensors: Advancements, applications, and future directions in molecular detection","authors":"Nidal El Biyari","doi":"10.1016/j.biotno.2026.01.002","DOIUrl":"10.1016/j.biotno.2026.01.002","url":null,"abstract":"<div><div>Surface Plasmon Resonance (SPR) biosensors have emerged as one of the most potent and adaptable methods for detecting molecular interactions in real-time and without labeling. SPR enables the accurate monitoring of biomolecular interactions in various contexts by detecting changes in refractive index near the sensor's surface. Over the last few decades, SPR technology has advanced dramatically, with improvements in sensor sensitivity, resolution, and throughput.</div><div>This study provides a comprehensive overview of SPR biosensors, highlighting recent advances in sensor technology, materials, and detection methodologies. We cover the fundamentals of SPR sensing and the factors that influence sensor performance, including metal selection, surface functionalization, and immobilization techniques. The report also looks at the wide range of applications for SPR biosensors, including drug development and illness diagnostics, as well as environmental monitoring and food safety.</div><div>The promise of SPR technology is further investigated by studying new advancements such as the incorporation of nanomaterials, microfluidics, and multi-analyte detection systems. We also explore the SPR biosensor's future directions, including existing limits and potential applications in customized medicine, point-of-care diagnostics, and quick environmental monitoring.</div><div>This review seeks to give a comprehensive overview of SPR biosensors, highlighting their potential to revolutionize molecular detection in a variety of disciplines, as well as outlining the obstacles and possibilities that lie ahead for their future development.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 22-43"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147278161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Between FAMEs-Qualitative and Biomass-Quantitative: A review study on oleaginous fungi 在名目-定性与生物量-定量之间：产油真菌研究综述。

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-02-19 DOI: 10.1016/j.biotno.2026.01.005

Kadhim Fadhil Kadhim , Inaam Mahmood Najem Alrubayae , Mohammed Hussein Minati

{"title":"Between FAMEs-Qualitative and Biomass-Quantitative: A review study on oleaginous fungi","authors":"Kadhim Fadhil Kadhim , Inaam Mahmood Najem Alrubayae , Mohammed Hussein Minati","doi":"10.1016/j.biotno.2026.01.005","DOIUrl":"10.1016/j.biotno.2026.01.005","url":null,"abstract":"<div><div>Energy scarcity remains a major global challenge, particularly in the transition toward sustainable and secure energy systems. Fossil energy plays a pivotal role in exacerbating environmental pollution. Consequently, modern bio-refineries and bioenergy have been crucial in utilizing biomass to produce a range of high-value bio-chemicals and biofuels, all in the effort to achieve a climate-neutral future. Fungal microbial lipids could represent essential alternative raw materials for biodiesel production, which may perhaps contribute to partially addressing the problem of declining global oil production. Oleaginous fungi accumulate lipids exceeding 20% of their dry biomass, predominantly as triacylglycerols comprising saturated and unsaturated fatty acids, which closely resemble fatty acids found in vegetable oils that are currently utilized in biodiesel production. Despite the significant advantages of these fungi, large-scale industrial production of fungal lipids has not yet been commercialized. One of the most important challenges that oleaginous fungi must overcome is their ability to produce very large quantities of biomass by using very cheap raw materials, in addition to the fact that these masses contain large quantities of oils of high quality to achieve economic feasibility. This review highlights the investment possibility of oleaginous fungi in biomass-based biodiesel production, their characterization as well as <em>in vitro</em> screening advantages, and indicates the need for further research to improve their industrial applications and increase their efficiency.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 58-74"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147313958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and production of a Bacillus mycoides Bioflocculant for sustainable effluent treatment 一种用于可持续污水处理的芽孢杆菌生物絮凝剂的特性和生产

Biotechnology Notes Pub Date : 2026-01-01 Epub Date: 2026-02-07 DOI: 10.1016/j.biotno.2026.01.001

Karthikeyan Harinisri, Balasubramanian Thamarai Selvi

{"title":"Characterization and production of a Bacillus mycoides Bioflocculant for sustainable effluent treatment","authors":"Karthikeyan Harinisri, Balasubramanian Thamarai Selvi","doi":"10.1016/j.biotno.2026.01.001","DOIUrl":"10.1016/j.biotno.2026.01.001","url":null,"abstract":"<div><div>Industrial effluent treatment relies on chemical coagulants, which incur higher costs and generate toxic sludge. This study isolated and identified bioflocculant-producing <em>Bacillus mycoides</em> (S39) strain and enhanced yield using one-factor-at-a-time optimization. Characterization studies of purified bioflocculant reveal a crystalline polysaccharide bearing amine, hydroxyl, and carboxylate groups that drive adsorption and polymer bridging. The bioflocculant exhibited flocculation activities of 95.56 % for textile effluent and 92.84 % for steel wastewater with Ca<sup>2+</sup> activation. Bioflocculant remained stable across pH and temperature. Kinetics followed a pseudo-first-order model, yielding rate constants, an optimized dosage, and assistance with sizing. Hemolysis (<5 %) and viability (>80 %) assays indicated non-cytotoxicity. The bioflocculant maintained performance ranged from 0.01 to 10 L, supporting S39 as a sustainable alternative to chemical coagulants. This study investigated the potential of <em>B</em>. <em>mycoides</em> bioflocculant, which showed promise as a sustainable and high-efficiency alternative to conventional coagulants and, to the best of our knowledge, the first kinetic and scale-relevant evaluation of this strain for wastewater treatment.</div></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"7 ","pages":"Pages 1-15"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0