Biotechnology Notes最新文献

筛选
英文 中文
Improving Spinach2-and Broccoli-based biosensors for single and double analytes 改进基于菠菜2和西兰花的单、双分析生物传感器
Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.01.001
Shuo-Fu Yuan , Hal S. Alper
{"title":"Improving Spinach2-and Broccoli-based biosensors for single and double analytes","authors":"Shuo-Fu Yuan ,&nbsp;Hal S. Alper","doi":"10.1016/j.biotno.2020.01.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2020.01.001","url":null,"abstract":"<div><p>The use of “Spinach”-based RNA sensors for the detection of small metabolites and proteins has received growing interest in the recent years. While this approach can be used <em>in vivo</em> for cell sensing and <em>in vitro</em> for microfluidic assays, their overall utility is limited as a result of a high magnesium ion dependence. Here, we alleviate this limitation through incorporating a human tRNA<sup>Lys3</sup> or an engineered viral F30 (a three-way junction RNA motif) scaffold to facilitate aptamer folding <em>in vitro</em> and improve the performance of selected streptavidin, tyrosine, and thrombin aptamers as exemplary cases. Furthermore, we demonstrate the use of a Broccoli aptamer scaffold in conjunction with the viral F30 to enable simultaneous sensing of two molecules in a logic gate type fashion. Our proof-of-concept results demonstrate the ability to redesign these aptamer sensors for improved brightness as well as signal stability without the need for high magnesium—both traits that can further enhance downstream screening applications.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"1 ","pages":"Pages 2-8"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biotno.2020.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91684844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Directed evolution of VanR biosensor specificity in yeast 酵母中VanR生物传感器特异性的定向进化
Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.01.002
Vasil D'ambrosio, S. Pramanik, Kati Goroncy, Tadas Jakočiūnas, David Schönauer, M. Davari, U. Schwaneberg, J. Keasling, M. K. Jensen
{"title":"Directed evolution of VanR biosensor specificity in yeast","authors":"Vasil D'ambrosio, S. Pramanik, Kati Goroncy, Tadas Jakočiūnas, David Schönauer, M. Davari, U. Schwaneberg, J. Keasling, M. K. Jensen","doi":"10.1016/j.biotno.2020.01.002","DOIUrl":"https://doi.org/10.1016/j.biotno.2020.01.002","url":null,"abstract":"","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83494182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信