Biotechnology Notes最新文献_第3页

Potential application of menadione for antimicrobial coating of surgical sutures 甲萘醌在外科缝合线抗菌涂层中的潜在应用

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.001

Cheng Hong Yap , See Khai Lim , Yun Li Chan , Chin Fei Chee , Sun Tee Tay

{"title":"Potential application of menadione for antimicrobial coating of surgical sutures","authors":"Cheng Hong Yap , See Khai Lim , Yun Li Chan , Chin Fei Chee , Sun Tee Tay","doi":"10.1016/j.biotno.2023.02.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.02.001","url":null,"abstract":"<div><p>Staphylococcal-associated surgical site infections (SSI) are common nosocomial infections in healthcare facilities worldwide. The use of antiseptic-coated sutures has been recommended to minimise the risk of SSI in clinical settings. However, as there has been a growing concern over antibiotic resistance resulting from antiseptic usage, development of antimicrobial sutures using alternative compounds is necessary. In this study, menadione (2-methyl-1,4-napthoquinone), also known as Vitamin K<sub>3,</sub> was evaluated as a potential antimicrobial compound for suture coating. The anti-staphylococcal activity of menadione was assessed using microbroth dilution method and biofilm inhibition assays. The low menadione minimum biofilm inhibitory concentration values against both methicillin-susceptible and -resistant <em>S. aureus</em> strains indicate its inhibitory activity against staphylococcal biofilm. Menadione-coated sutures were prepared by dip-coating surgical sutures in slurries containing poly(D,L-lactide-co-glycolide) polymers (either 65:35 or 75:25) and calcium stearate. Zone of inhibition assays showed dose-dependent antimicrobial effects of the sutures up to four days. A ∼3 log10 colony forming unit/ml reduction of adherent bacteria (p < 0.05) on the sutures was demonstrated via bacterial adherence assays. The integrity and tensile strength of the sutures were unaffected by the coating procedure. In view of the increased antibiotic resistance and limited antimicrobials, menadione may be potentially useful for antimicrobial coating of surgical sutures.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 20-27"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Synthetic biology in Indonesia: Potential and projection in a country with mega biodiversity 印度尼西亚的合成生物学:在一个拥有巨大生物多样性的国家的潜力和预测

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.002

Immanuel Sanka , Ali Budhi Kusuma , Faustina Martha , Andre Hendrawan , Ihsan Tria Pramanda , Adhityo Wicaksono , Afif Pranaya Jati , Maulida Mazaya , Ari Dwijayanti , Nurul Izzati , Muhammad Farhan Maulana , Aulia Reski Widyaningrum

引用次数: 1

Decoding dye degradation: Microbial remediation of textile industry effluents 解码染料降解:纺织工业废水的微生物修复

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.10.001

Soumyajit Das , Lubhan Cherwoo , Ravinder Singh

{"title":"Decoding dye degradation: Microbial remediation of textile industry effluents","authors":"Soumyajit Das , Lubhan Cherwoo , Ravinder Singh","doi":"10.1016/j.biotno.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.10.001","url":null,"abstract":"<div><p>The extensive use of chemical dyes, primarily Azo and anthraquinone dyes, in textiles has resulted in their alarming release into the environment by textile industries. The introduction of heavy metals into these dyes leads to an increase in Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and water toxicity. Conventional physicochemical methods for treating textile effluents are costly and energy-intensive. Here introduction of new strategies is eminent, microbial bioremediation for the biodegradation and detoxification of these hazardous dyes, possesses as an innovative solution for the existing problem, discussed are specific groups of bacteria, fungi, and algae which could be one of the potential decolorizing agents that could replace the majority of other expensive processes in textile wastewater treatment by using enzymes like peroxidase, laccase, and azoreductase. These enzymes catalyzes chemical reactions that break down the dye molecules into less harmful substances. Additionally, novel strategies and advancements to enhance the effectiveness of these microbes and their products are comprehensively discussed.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 64-76"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000065/pdfft?md5=21a407f49cff5a595dbe1b0a2c0c47b0&pid=1-s2.0-S2665906923000065-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol 利用甲醇作为辅助底物生产(R)-1,3-丁二醇的大肠杆菌代谢工程

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.005

Qing Sun , Dehua Liu , Zhen Chen

{"title":"Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol","authors":"Qing Sun , Dehua Liu , Zhen Chen","doi":"10.1016/j.biotno.2023.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.005","url":null,"abstract":"<div><p>Due to its abundance, cost-effectiveness, and high reducibility, methanol has gained considerable attention in the biomanufacturing industry as a nonfood feedstock for the production of value-added chemicals. The range of chemicals that can be derived from methanol, however, remains constrained and is currently in the concept validation phase. This study aimed to develop and evaluate a hybrid methanol assimilation pathway in <em>Escherichia coli</em> to improve the production of (<em>R</em>)-1,3-butanediol ((<em>R</em>)-1,3-BDO) by utilizing methanol and sugars as co-substrates. By combining the methanol dehydrogenase (MDH) from the prokaryotes with the dihydroxyacetone synthase (DAS) from the eukaryotes, the hybrid pathway facilitates methanol conversion into the central metabolism while generating NADH at the same time. Through pathway optimization and targeted gene deletions, we have successfully developed an <em>E. coli</em> strain capable of producing 5.79 g/L (<em>R</em>)-1,3-BDO in shake flask experiments and 13.71 g/L (<em>R</em>)-1,3-BDO with a yield of 0.35 C-mol/C-mol in batch fermentation using methanol and glucose as co-substrates. Our study also showed the incorporation of <sup>13</sup>C-methanol into cellular intermediates and an increase in NAD(P)H concentration, confirming the role of methanol as a co-substrate and supplier of NADH. In addition, our study also demonstrated the co-utilization of methanol with xylose for the production of (<em>R</em>)-1,3-BDO, expanding the substrate spectrum for sustainable 1,3-BDO production.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 104-111"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000119/pdfft?md5=6b46fabdf4fad0fa9e2396f04c4a5f59&pid=1-s2.0-S2665906923000119-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138564409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae 双质粒相互作用促进缬草烯在酿酒酵母中的积累

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.004

Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li

{"title":"Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae","authors":"Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li","doi":"10.1016/j.biotno.2023.12.004","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.004","url":null,"abstract":"<div><p>Plasmids are one of the most commonly used basic tools in the construction of microbial cell factories, the use of which individually or in pairs play an important role in the expression of exogenous gene modules. However, little attention has been paid to the interactions of plasmid-plasmid and plasmid-host in the widespread use of the double plasmid system. In this study, we demonstrated that dual-plasmid interactions facilitated to cell growth and product accumulation in <em>Saccharomyces cerevisiae</em>. The strain containing both the expression plasmid pEV (a plasmid carrying the gene encoding valencene synthase) and the assistant plasmid pI (an empty plasmid expressing no extra gene) showed a significant improvement in relative growth rate, biomass and valencene production compared to the strain containing only the pEV plasmid. The transcriptional level analysis revealed an up-regulated expression of specific gene on the expression plasmid pEV stimulated by the assistant plasmid pI in the dual-plasmid interactions. Further investigations demonstrated the essential roles of the promoters of the expression plasmid pEV and the CEN/ARS element of the assistant plasmid pI in the dual-plasmid interactions. Combined with the results of predicted nucleosome occupancy, a response model of interaction based on the key T(n)C and CEN/ARS element was established. Moreover, the transformation order of the two plasmids significantly affected the response effect, implying the dominance of plasmid pI in the dual-plasmid interactions. Our finding first demonstrated that dual plasmids regulate the gene expression through spatial interactions at DNA sequences level, which provides a new perspective for the development of microbial cell factories in future.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 127-134"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000168/pdfft?md5=2d34e1efc0f7c67c058f46ddba2d6840&pid=1-s2.0-S2665906923000168-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2nd symposium on engineering biology and BioFoundry 第二届工程生物学与生物铸造学术研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.004

Yuanli Gao , Chang Dong , Jiazhang Lian, Baojun Wang

引用次数: 0

Synthetic microbes and biocatalyst designs in Thailand 泰国的合成微生物和生物催化剂设计

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.003

Duangthip Trisrivirat , Ruchanok Tinikul , Pimchai Chaiyen

引用次数: 1

Synchronous efforts for burgeoning bioeconomy: The 4th international biodesign research conference and international symposium on development and application of modern biotechnology 同步努力，促进蓬勃发展的生物经济：第四届国际生物设计研究大会暨现代生物技术发展与应用国际研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.005

Qiaoning He, Huimin Yu, Shihui Yang

引用次数: 0

Harnessing gut cells for functional insulin production: Strategies and challenges 利用肠道细胞产生功能性胰岛素:策略和挑战

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.11.005

Kelvin Baafi, John C. March

{"title":"Harnessing gut cells for functional insulin production: Strategies and challenges","authors":"Kelvin Baafi, John C. March","doi":"10.1016/j.biotno.2022.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.11.005","url":null,"abstract":"<div><p>Reprogrammed glucose-responsive, insulin + cells (“<em>β</em>-like”) exhibit the potential to bypass the hurdles of exogenous insulin delivery in treating diabetes mellitus. Current cell-based therapies-transcription factor regulation, biomolecule-mediated enteric signaling, and transgenics - have demonstrated the promise of reprogramming either mature or progenitor gut cells into surrogate “<em>β</em>-like” cells. However, there are predominant challenges impeding the use of gut “<em>β</em>-like” cells as clinical replacements for insulin therapy. Reprogrammed “<em>β</em>-like” gut cells, even those of enteroendocrine origin, mostly do not exhibit glucose – potentiated insulin secretion. Despite the exceptionally low conversion rate of gut cells into surrogate “<em>β</em>-like” cells, the therapeutic quantity of gut “<em>β</em>-like” cells needed for normoglycemia has not even been established. There is also a lingering uncertainty regarding the functionality and bioavailability of gut derived insulin. Herein, we review the strategies, challenges, and opportunities in the generation of functional, reprogrammed “<em>β</em>-like” cells.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 7-13"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-conversion of organic wastes towards polyhydroxyalkanoates 将有机废物生物转化为聚羟基烷酸酯

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.006

Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye

{"title":"Bio-conversion of organic wastes towards polyhydroxyalkanoates","authors":"Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye","doi":"10.1016/j.biotno.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.006","url":null,"abstract":"<div><p>The bio-manufacturing of products with substantial commercial value, particularly polyhydroxyalkanoates (PHA), using cost-effective carbon sources through microorganisms, has garnered heightened attention from both the scientific community and industry over the past few decades. Opting for industrial PHA production from various organic wastes, spanning industrial, agricultural, municipal, and food-based sources, emerges as a wiser choice. This strategy not only eases the burden of recycling organic waste and curbs environmental pollution but also trims down PHA production costs, rendering these materials more competitive in commercial markets. In addition, PHAs are a family of renewable, environmentally friendly, fully biodegradable and biocompatible polyesters with a multitude of applications. This review provides an overview of recent developments in PHA production from organic wastes. It covers the optimization of diverse metabolic pathways for producing various types of PHA from organic waste sources, pre-treatment and downstream processing for PHA using unrelated organic wastes, and challenges in industrial production of PHA using unrelated organic waste feedstocks and the challenges faced in industrial PHA production from organic wastes, along with potential solutions. Lastly, this study suggests underlying research endeavors aimed at further enhancing of the feasibility of industrial PHA production from organic wastes as an alternative to current petroleum-based plastics in the near future.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 118-126"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000120/pdfft?md5=5995995eb74f3869768728151c55ef72&pid=1-s2.0-S2665906923000120-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138570577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0