Biotechnology Notes最新文献_第3页

Biotechnology Notes: A Year of Milestones

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.11.004

Matthew Wook Chang

引用次数: 0

Understanding virus retention mechanisms on protein a chromatography based on using different wash buffers – Evaluating the possibility for a generic wash buffer toolbox to improve virus clearance capacity 通过使用不同的清洗缓冲液，了解病毒在蛋白 a 层析上的滞留机制 - 评估通用清洗缓冲液工具箱的可能性，以提高病毒清除能力

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.03.001

Sandra Krause , Florian Capito , Verena Oeinck , Hendrik Flato , Holger Hoffmann , Ozan Ötes , Annette Berg

{"title":"Understanding virus retention mechanisms on protein a chromatography based on using different wash buffers – Evaluating the possibility for a generic wash buffer toolbox to improve virus clearance capacity","authors":"Sandra Krause , Florian Capito , Verena Oeinck , Hendrik Flato , Holger Hoffmann , Ozan Ötes , Annette Berg","doi":"10.1016/j.biotno.2024.03.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2024.03.001","url":null,"abstract":"<div><p>During manufacturing of mammalian-cell derived monoclonal antibodies (mAbs) virus clearance capacity of the downstream process has to be demonstrated. The protein A chromatography step typically achieves less than 4 log<sub>10</sub> and is not considered as a major contributing step. Having been successfully applied to host cell protein removal before, we used different wash buffers for three mAbs with two model viruses (Minute virus of mice and Murine leukemia virus) in series as well as separately to further understand major contributing interactions for virus retention and potentially design a generic toolbox of stringent wash buffers to be applied to various mAbs. Results indicate a major relevance of hydrophobic interaction for Murine leukemia virus (xMuLV) and mAb A, based on improved clearance for buffers additionally containing increased levels of hydrophobic compounds. This effect was less pronounced for Minute virus of mice (MVM), whereby hydrogen-bonds were expected to play a stronger role for this model virus. Additionally, electrostatic interactions presumably are more relevant for MVM retention compared to xMuLV under the conditions evaluated. A generic mAb and virus-independent stringent wash buffer toolbox could not be identified. However, based on our results a customized mAb and virus wash buffer design with improved virus clearance is possible, with here demonstrated log reduction increase by 1.3 log<sub>10</sub> for MVM and 2.2 log<sub>10</sub> for xMuLV for the protein A step compared to equilibration buffer alone.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 50-57"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000060/pdfft?md5=d90d78b9590b013af6078b5615adb6ad&pid=1-s2.0-S2665906924000060-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140348246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incorporating omics-based tools into endophytic fungal research 将基于omics的工具纳入内生真菌研究

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2023.12.006

Vinita Verma , Alok Srivastava , Sanjay Kumar Garg , Vijay Pal Singh , Pankaj Kumar Arora

{"title":"Incorporating omics-based tools into endophytic fungal research","authors":"Vinita Verma , Alok Srivastava , Sanjay Kumar Garg , Vijay Pal Singh , Pankaj Kumar Arora","doi":"10.1016/j.biotno.2023.12.006","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.006","url":null,"abstract":"<div><p>Fungal endophytes are valuable sources of bioactive compounds with diverse applications. The exploration of these compounds not only contributes to our understanding of ecological interactions but also holds promise for the development of novel products with agricultural, medicinal, and industrial significance. Continued exploration of fungal endophyte diversity and understanding the ecological roles of bioactive compounds present opportunities for new discoveries and applications. Omics techniques, which include genomics, transcriptomics, proteomics, and metabolomics, contribute to the discovery of novel bioactive compounds produced by fungal endophytes with their potential applications. The omics techniques play a critical role in unraveling the complex interactions between fungal endophytes and their host plants, providing valuable insights into the molecular mechanisms and potential applications of these relationships. This review provides an overview of how omics techniques contribute to the study of fungal endophytes.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 1-7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000259/pdfft?md5=222a86c032e0d932ffc20dd4a771b7fe&pid=1-s2.0-S2665906923000259-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139108011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of brain-derived neurotrophic factor and its recombinant pro-isoforms in depressive disorder: Open questions 脑源性神经营养因子及其重组原异构体在抑郁障碍中的作用：悬而未决的问题

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.09.001

Éllen F. Rodrigues , Ana L. Fachin , Mozart Marins , Felipe Britto Letieri , Rodrigo G. Stabeli , Renê O. Beleboni

引用次数: 0

The future of cell-free synthetic biology

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.11.001

Yuan Lu

引用次数: 0

Unlocking the potential of biosurfactants: Innovations in metabolic and genetic engineering for sustainable industrial and environmental solutions 释放生物表面活性剂的潜力：新陈代谢和基因工程创新：可持续的工业和环境解决方案

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.07.001

Sameer Chabhadiya , D.K. Acharya , Amitsinh Mangrola , Rupal Shah , Edwin A. Pithawala

{"title":"Unlocking the potential of biosurfactants: Innovations in metabolic and genetic engineering for sustainable industrial and environmental solutions","authors":"Sameer Chabhadiya , D.K. Acharya , Amitsinh Mangrola , Rupal Shah , Edwin A. Pithawala","doi":"10.1016/j.biotno.2024.07.001","DOIUrl":"10.1016/j.biotno.2024.07.001","url":null,"abstract":"<div><p>Biosurfactants, synthesized by microorganisms, hold potential for various industrial and environmental applications due to their surface-active properties and biodegradability. Metabolic and genetic engineering strategies enhance biosurfactant production by modifying microbial pathways and genetics. Strategies include optimizing biosurfactant biosynthesis pathways, expanding substrate utilization, and improving stress responses. Genetic engineering allows customization of biosurfactant characteristics to meet industrial needs. Notable examples include engineering <em>Pseudomonas aeruginosa</em> for enhanced rhamnolipid production and creating synthetic biosurfactant pathways in non-native hosts like <em>Escherichia coli</em>. CRISPR-Cas9 technology offers precise tools for genetic manipulation, enabling targeted gene disruption and promoter optimization to enhance biosurfactant production efficiency. Synthetic promoters enable precise control over biosurfactant gene expression, contributing to pathway optimization across diverse microbial hosts. The future of biosurfactant research includes sustainable bio-processing, customized biosurfactant engineering, and integration of artificial intelligence and systems biology. Advances in genetic and metabolic engineering will enable tailor-made biosurfactants for diverse applications, with potential for industrial-scale production and commercialization. Exploration of untapped microbial diversity may lead to novel biosurfactants with unique properties, expanding the versatility and sustainability of biosurfactant-based solutions.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 111-119"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000114/pdfft?md5=4bf3ad577ced0d7cd38282ad249883ae&pid=1-s2.0-S2665906924000114-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9 improves targeted knock-in efficiency in Aspergillus oryzae CRISPR/Cas9 提高了黑曲霉的定向基因敲入效率

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.03.002

Takehiko Todokoro, Yoji Hata, Hiroki Ishida

{"title":"CRISPR/Cas9 improves targeted knock-in efficiency in Aspergillus oryzae","authors":"Takehiko Todokoro, Yoji Hata, Hiroki Ishida","doi":"10.1016/j.biotno.2024.03.002","DOIUrl":"https://doi.org/10.1016/j.biotno.2024.03.002","url":null,"abstract":"<div><p><em>Aspergillus oryzae</em> is an important fungus in food and industrial enzyme production. In <em>A. oryzae</em>, targeted knock-in transformation is primarily limited to homologous recombination (HR)-based systems, in which non-homologous end-joining (NHEJ)-disruptant hosts are required. However, preparation of hosts and transformation templates for such systems is laborious, in addition to other disadvantages. In the present study, we examined alternative targeted knock-in mediated by CRISPR/Cas9, in which a microhomology-mediated end-joining (MMEJ) and single-strand annealing (SSA) repair system was employed. This approach enabled the efficient development of targeted knock-in transformants without host preparation using only a short homology template. We conclude that this new method could be applied to facilitate the transformation of <em>A. oryzae</em>, and will make it easier to acquire targeted knock-in transformants, especially from industrially important non-model strains.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 58-63"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000072/pdfft?md5=c3071d5a3a69f15bfd265d3655c1bb84&pid=1-s2.0-S2665906924000072-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140351146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling current breakthroughs in Scorodocarpus borneensis phytochemical therapeutics: A systematic review 黄芩生理化学疗法中的电流断裂：系统回顾

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.05.001

Muhammad Hamizan Zawawi , Siti Azhani-Amran , Zuraidah Abdullah , Sabreena Safuan

{"title":"Unraveling current breakthroughs in Scorodocarpus borneensis phytochemical therapeutics: A systematic review","authors":"Muhammad Hamizan Zawawi , Siti Azhani-Amran , Zuraidah Abdullah , Sabreena Safuan","doi":"10.1016/j.biotno.2024.05.001","DOIUrl":"10.1016/j.biotno.2024.05.001","url":null,"abstract":"<div><p><em>Scorodocarpus borneensis</em>, also known as the Kulim tree or Garlic tree, has been consumed by the local communities in Southeast Asia as traditional spice using its old leaves, stem bark, and seeds. The locals also used Kulim tree parts as conventional alternative to treat many diseases such as hemorrhoids, leprosy, diabetes, and diarrhea. However, there was limited scientific evidence to support these traditional claims. Therefore, this systematic review aims to present and evaluate a detailed overview of the phytochemical constituents of <em>S. borneensis</em> from previous existing studies, shedding light on their chemical composition, bioactivity, and potential applications. In addition, current studies regarding <em>S. borneensis</em> are still on a fundamental level. Hence, we aim that this review will reveal the research gap from the previous literature and provide an insight to implement a new research approach in the future. A literature search was conducted using four databases: ScienceDirect, Scopus, Web of Science, and PubMed. The articles were screened and data were extracted based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guideline. 8 studies satisfied the inclusion criteria that focused on the phytochemicals from <em>S. borneensis</em>. The major phytochemical compound reported was phenolic compound. S. borneensis also exhibits several therapeutic outcomes such as antioxidant, antimicrobial, and anticancer but current studies are not enough to support the claims regarding <em>S. borneensis</em> health benefit. In conclusion, this review highlights the current understanding of <em>S. borneensis’</em> phytochemical composition and its therapeutic applications which are important in preventing human diseases especially non-communicable diseases.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 100-110"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000084/pdfft?md5=2778f6f37bfba7a5ed673fe883e37908&pid=1-s2.0-S2665906924000084-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141401655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Chlorella vulgaris and Chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass 将小球藻和小球藻确定为将氨基葡萄糖生物转化为有价值生物质的可持续生物体

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.01.003

Hosam Elhalis , Mohamed Helmy , Sherilyn Ho , Sharon Leow , Yan Liu , Kumar Selvarajoo , Yvonne Chow

{"title":"Identifying Chlorella vulgaris and Chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass","authors":"Hosam Elhalis , Mohamed Helmy , Sherilyn Ho , Sharon Leow , Yan Liu , Kumar Selvarajoo , Yvonne Chow","doi":"10.1016/j.biotno.2024.01.003","DOIUrl":"https://doi.org/10.1016/j.biotno.2024.01.003","url":null,"abstract":"<div><p>Chitin is a major component of various wastes such as crustacean shells, filamentous fungi, and insects. Recently, food-safe biological and chemical processes converting chitin to glucosamine have been developed. Here, we studied microalgae that can uptake glucosamine as vital carbon and nitrogen sources for valuable alternative protein biomass. Utilizing data mining and bioinformatics analysis, we identified 29 species that contain the required enzymes for glucosamine to glucose conversion. The growth performance of the selected strains was examined, and glucosamine was used in different forms and concentrations. Glucose at a concentration of 2.5 g/L was required to initiate glucosamine metabolic degradation by <em>Chlorella vulgaris</em> and <em>Chlorella sorokiniana</em>. Glucosamine HCl and glucosamine phosphate showed maximum cell counts of about 8.5 and 9.0 log/mL for C. <em>sorokiniana</em> and <em>C. vulgaris</em> in 14 days, respectively. Enzymatic hydrolysis of glucosamine increased growth performance with <em>C. sorokiniana</em> by about 3 folds. The adapted strains were fast-growing and could double their dry biomasses during the same incubation time. In addition, adapted <em>C. sorokiniana</em> was able to tolerate three times glucosamine concentration in the medium. The study illustrated possible strategies for employing <em>C. sorokiniana</em> and <em>C. vulgaris</em> to convert glucosamine into valuable biomass in a more sustainable way.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 13-22"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000035/pdfft?md5=2d02dd1a924a37191fa8804cbe12d15c&pid=1-s2.0-S2665906924000035-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139494007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Therapeutic advancements in nanomedicine: The multifaceted roles of silver nanoparticles" "纳米医学的治疗进展：银纳米粒子的多方面作用"

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.05.002

Karthik K Karunakar , Binoy Varghese Cheriyan , Krithikeshvaran R , Gnanisha M , Abinavi B

{"title":"\"Therapeutic advancements in nanomedicine: The multifaceted roles of silver nanoparticles\"","authors":"Karthik K Karunakar , Binoy Varghese Cheriyan , Krithikeshvaran R , Gnanisha M , Abinavi B","doi":"10.1016/j.biotno.2024.05.002","DOIUrl":"10.1016/j.biotno.2024.05.002","url":null,"abstract":"<div><p>Nanotechnology has the advantages of enhanced bioactivity, reduced toxicity, target specificity, and sustained release and NPs can penetrate cell membranes. The small size of silver nanoparticles, AgNPs, large surface area, and unique physicochemical properties contribute to cell lysis and increased permeability of cell membranes used in the field of biomedicine. Functional precursors integrate with phytochemicals to create distinctive therapeutic properties and the stability of the nanoparticles can be enhanced by Surface coatings and encapsulation methods, The current study explores the various synthesis methods and characterization techniques of silver nanoparticles (AgNPs) and highlights their intrinsic activity in therapeutic applications, Anti-cancer activity noted at a concentration range of 5–50 μg/ml and angiogenesis is mitigated at a dosage range of 10–50 μg/ml, Diabetes is controlled within the same concentration. Wound healing is improved at concentrations of 10–50 μg/ml and with a typical range of 10–08 μg/ml for bacteria with antimicrobial capabilities. Advancement of silver nanoparticles with a focus on the future use of AgNPs-coated wound dressings and medical devices to decrease the risk of infection. Chemotherapeutic drugs can be administered by AgNPs, which reduces adverse effects and an improvement in treatment outcomes. AgNPs have been found to improve cell proliferation and differentiation, making them beneficial for tissue engineering and regenerative medicine. Our study highlights emerging patterns and developments in the field of medicine, inferring potential future paths.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 64-79"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000096/pdfft?md5=e8e08526b06a4c8603d010ed4bfdd106&pid=1-s2.0-S2665906924000096-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0