Biotechnology Notes最新文献_第4页

Engineered microbial consortia for next-generation feedstocks 用于下一代原料的工程微生物联合体

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.01.002

Gazi Sakir Hossain , Yuanmei Liang , Jee Loon Foo , Matthew Wook Chang

引用次数: 0

A comprehensive study on anaerobic digestion of organic solid waste: A review on configurations, operating parameters, techno-economic analysis and current trends 有机固体废物厌氧消化综合研究：关于配置、运行参数、技术经济分析和当前趋势的综述

Biotechnology Notes Pub Date : 2024-01-01 DOI: 10.1016/j.biotno.2024.02.001

D.Jaya Prasanna Kumar , Ranjeet Kumar Mishra , Sampath Chinnam , Prakash Binnal , Naveen Dwivedi

{"title":"A comprehensive study on anaerobic digestion of organic solid waste: A review on configurations, operating parameters, techno-economic analysis and current trends","authors":"D.Jaya Prasanna Kumar , Ranjeet Kumar Mishra , Sampath Chinnam , Prakash Binnal , Naveen Dwivedi","doi":"10.1016/j.biotno.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2024.02.001","url":null,"abstract":"<div><p>The excessive discharge and accumulation of solid organic waste into the environment is of severe concern across the globe. Thus, an efficient waste management system is important to mitigate health risks to humans, minimize harmful impacts on the environment, and ensure a sustainable ecosystem. The organic waste is converted into value-added products either using microorganisms or heat energy; these methods are commonly known as biochemical and thermochemical techniques. The biochemical process has the advantage of higher selectivity of the products and lower processing temperatures. The principal conversion processes of this category are fermentation and anaerobic digestion (AD). This review article focuses on AD, a potential method for treating organic waste and creating a variety of products with added value. Here we present the digestibility of various organic wastes, the role of microorganisms, the decomposition process, co-substrates, digester designs, biogas yields, by-products, environmental impacts, and overall techno-economical effectiveness of the process. Further, this review offers insights into new directions for AD for waste treatment and future research without compromising the overall feasibility and environmental sustainability.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"5 ","pages":"Pages 33-49"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906924000047/pdfft?md5=697f3bc9004f1454bf22f245f86a3bf8&pid=1-s2.0-S2665906924000047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140016320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and bioactive component analysis of filamentous bacterium XJ-16 丝状细菌XJ-16的鉴定及活性成分分析

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.03.001

Ke Jiang , Ruoxuan Bai , Xianglian Luo , Ting Gao , Fangxu Xu , Hongxin Zhao , Dianpeng Zhang

{"title":"Characterization and bioactive component analysis of filamentous bacterium XJ-16","authors":"Ke Jiang , Ruoxuan Bai , Xianglian Luo , Ting Gao , Fangxu Xu , Hongxin Zhao , Dianpeng Zhang","doi":"10.1016/j.biotno.2023.03.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.03.001","url":null,"abstract":"<div><p>Actinomycetes, which can produce a variety of bioactive compounds in the metabolic process, is one of the important sources of novel drugs, enzymes, anti-tumor drugs and enzyme inhibitors. It has been the focus of researchers to find and develop <em>Actinomycetes</em> with special characters. Strain XJ-16 is a blue alkali-resistant filamentous bacterium with high antimicrobial activity isolated from saline-alkali land of Xinjiang. Based on the classification, the enzyme production, metabolite antibacterial activity, and antibacterial substance isolation of XJ-16 were explored. which showed that XJ-16 belongs to the blue group of <em>Streptomyces</em> sp, and it can secrete cellulase, lipase, urease, protease, catalase and oxidase during metabolism. In addition, the bacteriostatic substance secreted by the strain XJ-16 showed inhibitory effects against both Gram-positive and Gram-negative bacteria, as well as the yeast <em>Candida albicans</em>. Then it was found that the bacteriostasis produced by XJ-16 has strong tolerance to acid, weak tolerance to alkali, and easy to be inactivated. After tested by HPLC, the retention time of antimicrobial substance was 13.261 min. This study provides new research ideas and theoretical support for searching for new antibacterial compounds and further developing the blue alkaline <em>Actinomycete</em> XJ-16.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 49-57"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49731575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suitability evaluation of toehold switch and EXPAR for cell-free MicroRNA biosensor development 支点开关和EXPAR在无细胞MicroRNA生物传感器开发中的适用性评价

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.003

Caroline E. Copeland , Yong-Chan Kwon

{"title":"Suitability evaluation of toehold switch and EXPAR for cell-free MicroRNA biosensor development","authors":"Caroline E. Copeland , Yong-Chan Kwon","doi":"10.1016/j.biotno.2023.11.003","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.003","url":null,"abstract":"<div><p>The development of a robust and cost-effective sensing platform for microRNA (miRNA) is of paramount importance in detecting and monitoring various diseases. Current miRNA detection methods are marred by low accuracy, high cost, and instability. The toehold switch riboregulator has shown promising results in detecting viral RNAs integrated with the freeze-dried cell-free system (CFS). This study aimed to leverage the toehold switch technology and portability to detect miRNA in the CFS and to incorporate the exponential amplification reaction (EXPAR) to bring the detection to clinically relevant levels. We assessed various EXPAR DNA templates under different conditions to enhance the accuracy of the sensing platform. Furthermore, different structures of toehold switches were tested with either high-concentration synthetic miRNA or EXPAR product to assess sensitivity. Herein, we elucidated the mechanisms of the toehold switch and EXPAR, presented the findings of these optimizations, and discussed the potential benefits and drawbacks of their combined use.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 83-89"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000090/pdfft?md5=61d37cf4a5204994790dfb4c07164302&pid=1-s2.0-S2665906923000090-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138439364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2023 The 1st National Competition of “Bloomag Cup” in CO2 capture, conversion, and utilization 2023 年首届 "布鲁马格杯 "全国二氧化碳捕获、转化和利用竞赛

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.001

Ziman Chen, Dan Wang, Zhenyi Wu, Yongqin Lv

{"title":"2023 The 1st National Competition of “Bloomag Cup” in CO2 capture, conversion, and utilization","authors":"Ziman Chen, Dan Wang, Zhenyi Wu, Yongqin Lv","doi":"10.1016/j.biotno.2023.12.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.001","url":null,"abstract":"<div><p>The inaugural National Competition for Carbon Dioxide Capture, Conversion and Utilization Innovation (“Bloomag Cup”) was successfully held on July 30, 2023 in Beijing. This competition was initiated by Professor Tianwei Tan and Prof. Yongqin Lv from Beijing University of Chemical Technology (BUCT), and jointly organized by BUCT and Chongqing University. The competition is slated for annual recurrence, with a rotational hosting arrangement involving various academic institutions. The ongoing competition underscores the primacy of pioneering and exploratory facets inherent to technological innovation. Its principal objective is to catalyze the development of foundational and cutting-edge technological competencies within the realm of CO<sub>2</sub> capture, conversion, and utilization. The overarching goals encompass identifying promising technological breakthroughs, fostering emerging talent in scientific and technological innovation, facilitating high-quality sustainable economic growth within China, and actively contributing to global efforts towards peak carbon emissions and achieving sustainable development goals for humanity. This inaugural Bloomag Cup saw wide participation from students and researchers, generating fruitful discussions that advance the nascent field. It is hoped this competition will continue cultivating solutions that help mitigate anthropogenic climate change through innovative carbon dioxide technologies.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 112-117"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000132/pdfft?md5=e434bed0478aec98b2cd4b2862b56c4d&pid=1-s2.0-S2665906923000132-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138564404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential application of menadione for antimicrobial coating of surgical sutures 甲萘醌在外科缝合线抗菌涂层中的潜在应用

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.001

Cheng Hong Yap , See Khai Lim , Yun Li Chan , Chin Fei Chee , Sun Tee Tay

{"title":"Potential application of menadione for antimicrobial coating of surgical sutures","authors":"Cheng Hong Yap , See Khai Lim , Yun Li Chan , Chin Fei Chee , Sun Tee Tay","doi":"10.1016/j.biotno.2023.02.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.02.001","url":null,"abstract":"<div><p>Staphylococcal-associated surgical site infections (SSI) are common nosocomial infections in healthcare facilities worldwide. The use of antiseptic-coated sutures has been recommended to minimise the risk of SSI in clinical settings. However, as there has been a growing concern over antibiotic resistance resulting from antiseptic usage, development of antimicrobial sutures using alternative compounds is necessary. In this study, menadione (2-methyl-1,4-napthoquinone), also known as Vitamin K<sub>3,</sub> was evaluated as a potential antimicrobial compound for suture coating. The anti-staphylococcal activity of menadione was assessed using microbroth dilution method and biofilm inhibition assays. The low menadione minimum biofilm inhibitory concentration values against both methicillin-susceptible and -resistant <em>S. aureus</em> strains indicate its inhibitory activity against staphylococcal biofilm. Menadione-coated sutures were prepared by dip-coating surgical sutures in slurries containing poly(D,L-lactide-co-glycolide) polymers (either 65:35 or 75:25) and calcium stearate. Zone of inhibition assays showed dose-dependent antimicrobial effects of the sutures up to four days. A ∼3 log10 colony forming unit/ml reduction of adherent bacteria (p < 0.05) on the sutures was demonstrated via bacterial adherence assays. The integrity and tensile strength of the sutures were unaffected by the coating procedure. In view of the increased antibiotic resistance and limited antimicrobials, menadione may be potentially useful for antimicrobial coating of surgical sutures.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 20-27"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49757647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Synthetic biology in Indonesia: Potential and projection in a country with mega biodiversity 印度尼西亚的合成生物学:在一个拥有巨大生物多样性的国家的潜力和预测

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.002

Immanuel Sanka , Ali Budhi Kusuma , Faustina Martha , Andre Hendrawan , Ihsan Tria Pramanda , Adhityo Wicaksono , Afif Pranaya Jati , Maulida Mazaya , Ari Dwijayanti , Nurul Izzati , Muhammad Farhan Maulana , Aulia Reski Widyaningrum

引用次数: 1

Decoding dye degradation: Microbial remediation of textile industry effluents 解码染料降解:纺织工业废水的微生物修复

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.10.001

Soumyajit Das , Lubhan Cherwoo , Ravinder Singh

{"title":"Decoding dye degradation: Microbial remediation of textile industry effluents","authors":"Soumyajit Das , Lubhan Cherwoo , Ravinder Singh","doi":"10.1016/j.biotno.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.10.001","url":null,"abstract":"<div><p>The extensive use of chemical dyes, primarily Azo and anthraquinone dyes, in textiles has resulted in their alarming release into the environment by textile industries. The introduction of heavy metals into these dyes leads to an increase in Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and water toxicity. Conventional physicochemical methods for treating textile effluents are costly and energy-intensive. Here introduction of new strategies is eminent, microbial bioremediation for the biodegradation and detoxification of these hazardous dyes, possesses as an innovative solution for the existing problem, discussed are specific groups of bacteria, fungi, and algae which could be one of the potential decolorizing agents that could replace the majority of other expensive processes in textile wastewater treatment by using enzymes like peroxidase, laccase, and azoreductase. These enzymes catalyzes chemical reactions that break down the dye molecules into less harmful substances. Additionally, novel strategies and advancements to enhance the effectiveness of these microbes and their products are comprehensively discussed.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 64-76"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000065/pdfft?md5=21a407f49cff5a595dbe1b0a2c0c47b0&pid=1-s2.0-S2665906923000065-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol 利用甲醇作为辅助底物生产(R)-1,3-丁二醇的大肠杆菌代谢工程

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.005

Qing Sun , Dehua Liu , Zhen Chen

{"title":"Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol","authors":"Qing Sun , Dehua Liu , Zhen Chen","doi":"10.1016/j.biotno.2023.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.005","url":null,"abstract":"<div><p>Due to its abundance, cost-effectiveness, and high reducibility, methanol has gained considerable attention in the biomanufacturing industry as a nonfood feedstock for the production of value-added chemicals. The range of chemicals that can be derived from methanol, however, remains constrained and is currently in the concept validation phase. This study aimed to develop and evaluate a hybrid methanol assimilation pathway in <em>Escherichia coli</em> to improve the production of (<em>R</em>)-1,3-butanediol ((<em>R</em>)-1,3-BDO) by utilizing methanol and sugars as co-substrates. By combining the methanol dehydrogenase (MDH) from the prokaryotes with the dihydroxyacetone synthase (DAS) from the eukaryotes, the hybrid pathway facilitates methanol conversion into the central metabolism while generating NADH at the same time. Through pathway optimization and targeted gene deletions, we have successfully developed an <em>E. coli</em> strain capable of producing 5.79 g/L (<em>R</em>)-1,3-BDO in shake flask experiments and 13.71 g/L (<em>R</em>)-1,3-BDO with a yield of 0.35 C-mol/C-mol in batch fermentation using methanol and glucose as co-substrates. Our study also showed the incorporation of <sup>13</sup>C-methanol into cellular intermediates and an increase in NAD(P)H concentration, confirming the role of methanol as a co-substrate and supplier of NADH. In addition, our study also demonstrated the co-utilization of methanol with xylose for the production of (<em>R</em>)-1,3-BDO, expanding the substrate spectrum for sustainable 1,3-BDO production.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 104-111"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000119/pdfft?md5=6b46fabdf4fad0fa9e2396f04c4a5f59&pid=1-s2.0-S2665906923000119-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138564409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae 双质粒相互作用促进缬草烯在酿酒酵母中的积累

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.004

Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li

{"title":"Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae","authors":"Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li","doi":"10.1016/j.biotno.2023.12.004","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.004","url":null,"abstract":"<div><p>Plasmids are one of the most commonly used basic tools in the construction of microbial cell factories, the use of which individually or in pairs play an important role in the expression of exogenous gene modules. However, little attention has been paid to the interactions of plasmid-plasmid and plasmid-host in the widespread use of the double plasmid system. In this study, we demonstrated that dual-plasmid interactions facilitated to cell growth and product accumulation in <em>Saccharomyces cerevisiae</em>. The strain containing both the expression plasmid pEV (a plasmid carrying the gene encoding valencene synthase) and the assistant plasmid pI (an empty plasmid expressing no extra gene) showed a significant improvement in relative growth rate, biomass and valencene production compared to the strain containing only the pEV plasmid. The transcriptional level analysis revealed an up-regulated expression of specific gene on the expression plasmid pEV stimulated by the assistant plasmid pI in the dual-plasmid interactions. Further investigations demonstrated the essential roles of the promoters of the expression plasmid pEV and the CEN/ARS element of the assistant plasmid pI in the dual-plasmid interactions. Combined with the results of predicted nucleosome occupancy, a response model of interaction based on the key T(n)C and CEN/ARS element was established. Moreover, the transformation order of the two plasmids significantly affected the response effect, implying the dominance of plasmid pI in the dual-plasmid interactions. Our finding first demonstrated that dual plasmids regulate the gene expression through spatial interactions at DNA sequences level, which provides a new perspective for the development of microbial cell factories in future.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 127-134"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000168/pdfft?md5=2d34e1efc0f7c67c058f46ddba2d6840&pid=1-s2.0-S2665906923000168-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0