Identifying Chlorella vulgaris and Chlorella sorokiniana as sustainable organisms to bioconvert glucosamine into valuable biomass

Abstract

Chitin is a major component of various wastes such as crustacean shells, filamentous fungi, and insects. Recently, food-safe biological and chemical processes converting chitin to glucosamine have been developed. Here, we studied microalgae that can uptake glucosamine as vital carbon and nitrogen sources for valuable alternative protein biomass. Utilizing data mining and bioinformatics analysis, we identified 29 species that contain the required enzymes for glucosamine to glucose conversion. The growth performance of the selected strains was examined, and glucosamine was used in different forms and concentrations. Glucose at a concentration of 2.5 g/L was required to initiate glucosamine metabolic degradation by Chlorella vulgaris and Chlorella sorokiniana. Glucosamine HCl and glucosamine phosphate showed maximum cell counts of about 8.5 and 9.0 log/mL for C. sorokiniana and C. vulgaris in 14 days, respectively. Enzymatic hydrolysis of glucosamine increased growth performance with C. sorokiniana by about 3 folds. The adapted strains were fast-growing and could double their dry biomasses during the same incubation time. In addition, adapted C. sorokiniana was able to tolerate three times glucosamine concentration in the medium. The study illustrated possible strategies for employing C. sorokiniana and C. vulgaris to convert glucosamine into valuable biomass in a more sustainable way.