Biotechnology Notes最新文献_第5页

2nd symposium on engineering biology and BioFoundry 第二届工程生物学与生物铸造学术研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.004

Yuanli Gao , Chang Dong , Jiazhang Lian, Baojun Wang

引用次数: 0

Synthetic microbes and biocatalyst designs in Thailand 泰国的合成微生物和生物催化剂设计

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.003

Duangthip Trisrivirat , Ruchanok Tinikul , Pimchai Chaiyen

引用次数: 1

Synchronous efforts for burgeoning bioeconomy: The 4th international biodesign research conference and international symposium on development and application of modern biotechnology 同步努力，促进蓬勃发展的生物经济：第四届国际生物设计研究大会暨现代生物技术发展与应用国际研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.005

Qiaoning He, Huimin Yu, Shihui Yang

引用次数: 0

Harnessing gut cells for functional insulin production: Strategies and challenges 利用肠道细胞产生功能性胰岛素:策略和挑战

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.11.005

Kelvin Baafi, John C. March

{"title":"Harnessing gut cells for functional insulin production: Strategies and challenges","authors":"Kelvin Baafi, John C. March","doi":"10.1016/j.biotno.2022.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.11.005","url":null,"abstract":"<div>Reprogrammed glucose-responsive, insulin + cells (“β-like”) exhibit the potential to bypass the hurdles of exogenous insulin delivery in treating diabetes mellitus. Current cell-based therapies-transcription factor regulation, biomolecule-mediated enteric signaling, and transgenics - have demonstrated the promise of reprogramming either mature or progenitor gut cells into surrogate “β-like” cells. However, there are predominant challenges impeding the use of gut “β-like” cells as clinical replacements for insulin therapy. Reprogrammed “β-like” gut cells, even those of enteroendocrine origin, mostly do not exhibit glucose – potentiated insulin secretion. Despite the exceptionally low conversion rate of gut cells into surrogate “β-like” cells, the therapeutic quantity of gut “β-like” cells needed for normoglycemia has not even been established. There is also a lingering uncertainty regarding the functionality and bioavailability of gut derived insulin. Herein, we review the strategies, challenges, and opportunities in the generation of functional, reprogrammed “β-like” cells.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 7-13"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-conversion of organic wastes towards polyhydroxyalkanoates 将有机废物生物转化为聚羟基烷酸酯

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.006

Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye

{"title":"Bio-conversion of organic wastes towards polyhydroxyalkanoates","authors":"Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye","doi":"10.1016/j.biotno.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.006","url":null,"abstract":"<div>The bio-manufacturing of products with substantial commercial value, particularly polyhydroxyalkanoates (PHA), using cost-effective carbon sources through microorganisms, has garnered heightened attention from both the scientific community and industry over the past few decades. Opting for industrial PHA production from various organic wastes, spanning industrial, agricultural, municipal, and food-based sources, emerges as a wiser choice. This strategy not only eases the burden of recycling organic waste and curbs environmental pollution but also trims down PHA production costs, rendering these materials more competitive in commercial markets. In addition, PHAs are a family of renewable, environmentally friendly, fully biodegradable and biocompatible polyesters with a multitude of applications. This review provides an overview of recent developments in PHA production from organic wastes. It covers the optimization of diverse metabolic pathways for producing various types of PHA from organic waste sources, pre-treatment and downstream processing for PHA using unrelated organic wastes, and challenges in industrial production of PHA using unrelated organic waste feedstocks and the challenges faced in industrial PHA production from organic wastes, along with potential solutions. Lastly, this study suggests underlying research endeavors aimed at further enhancing of the feasibility of industrial PHA production from organic wastes as an alternative to current petroleum-based plastics in the near future.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 118-126"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000120/pdfft?md5=5995995eb74f3869768728151c55ef72&pid=1-s2.0-S2665906923000120-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138570577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational engineering approaches for establishing insect olfaction reporters in yeast 在酵母中建立昆虫嗅觉报告基因的合理工程方法

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.002

Emma Elise Hoch-Schneider, Tatyana Saleski, Emil D. Jensen, Michael Krogh Jensen

{"title":"Rational engineering approaches for establishing insect olfaction reporters in yeast","authors":"Emma Elise Hoch-Schneider, Tatyana Saleski, Emil D. Jensen, Michael Krogh Jensen","doi":"10.1016/j.biotno.2023.11.002","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.002","url":null,"abstract":"<div>Insect olfaction directly impacts insect behavior and thus is an important consideration in the development of smart farming tools and in integrated pest management strategies. Insect olfactory receptors (ORs) have been traditionally studied using Drosophila empty neuron systems or with expression and functionalization in HEK293 cells or Xenopus laevis oocytes. Recently, the yeast Saccharomyces cerevisiae (S. cerevisiae) has emerged as a promising chassis for the functional expression of heterologous seven transmembrane receptors. S. cerevisiae provides a platform for the cheap and high throughput study of these receptors and potential deorphanization. In this study, we explore the foundations of a scalable yeast-based platform for the functional expression of insect olfactory receptors by employing a genetically encoded calcium sensor for quantitative evaluation of fluorescence and optimized experimental parameters for enhanced functionality. While the co-receptor of insect olfactory receptors remains non-functional in our yeast-based system, we thoroughly evaluated various experimental variables and identified future research directions for establishing an OR platform in S. cerevisiae.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 90-99"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000089/pdfft?md5=d694b5387164088876d639cb0d7fc7f5&pid=1-s2.0-S2665906923000089-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138467299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SynBio Africa's story from the grassroots, the present, and the future 非洲合成生物的故事从基层，现在和未来

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.11.003

Geoffrey Otim , Sandra Matinyi , Erikan Baluku , Ivy S.G. Chimulwa , George P. Magoola , Alex Katumba , Stephen Mukuze , Alex Kyabarongo , Stephen O. Opiyo

{"title":"SynBio Africa's story from the grassroots, the present, and the future","authors":"Geoffrey Otim , Sandra Matinyi , Erikan Baluku , Ivy S.G. Chimulwa , George P. Magoola , Alex Katumba , Stephen Mukuze , Alex Kyabarongo , Stephen O. Opiyo","doi":"10.1016/j.biotno.2022.11.003","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.11.003","url":null,"abstract":"<div>SynBio Africa is a forum for researchers, students, citizen scientists, policymakers and the public to convene and develop successful pathways for the propagation of synthetic biology technologies, products, and services throughout Africa. Our vision is to have a healthy, safe, and sustainable world through synthetic biology. In Africa, synthetic biology has the potential to greatly contribute to national development agenda through the following ways: i) by anchoring a sustainable bioeconomy; ii) by helping develop innovative medicines; iii) by reducing pollution, and iv) by increasing crop production to reduce hunger. However, there is little to no information on synthetic biology and its regulatory policies in Africa. Across the continent, scientists, policy makers, researchers and others are still working in silos—only partaking in consultative meetings to try and develop a set of unified policy guidelines. SynBio Africa is therefore proposing to establish the first Center of Excellence in Synthetic Biology in Africa with six themes, namely: research, capacity development, innovation hub, biosafety and biosecurity, and bioinformatics and data science, and one-health. Accordingly, SynBio Africa will work with collaborators from government and non-governmental organizations, the public and private sectors, and educational institutions from Uganda, Africa, and around the world to implement these six themes.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Over-expression of α-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox 利用金门DNA组装工具箱进行脂质体耶氏菌代谢工程对α-双abolene的过表达

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.12.005

Walid M. El-Sharoud , Samar A. Zalma , Leonardo Rios-Solis , Rodrigo Ledesma-Amaro

{"title":"Over-expression of α-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox","authors":"Walid M. El-Sharoud , Samar A. Zalma , Leonardo Rios-Solis , Rodrigo Ledesma-Amaro","doi":"10.1016/j.biotno.2022.12.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.12.005","url":null,"abstract":"<div>Yarrowia lipolytica is a modern workhorse for biotechnology that is amenable to genetic manipulations and can produce high levels of various enzymes. The present study was designed to engineer Y. lipolytica for the overexpression of α-bisabolene, a valuable biofuel precursor and pharmaceutical, making use of this yeast's ability to accumulate lipids, and with the use of a golden gate DNA assembly (GG) toolbox. By transforming Y. lipolytica with a GG genetic construct involving truncated 3-hydroxy-3-methyl-glutaryle coenzyme A reductase (tHMG) and α-bisabolene synthase (Bis) genes controlled by the strong TEF promoter and Lip2 terminator, the engineered yeast was able to produce 489 mg l−1 of α-bisabolene. This was increased to 816 mg l−1 by transforming a lipid-over-accumulating Y. lipolytica strain with the same genetic construct. Higher production titers of up to 1243 mg l−1 could be also achieved by varying the culture conditions of the transformed strains.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 14-19"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49761128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The electrical transport and antibacterial properties of Fe doped MgO nanoparticles synthesized by a soft chemical technique 软化学技术合成的掺铁氧化镁纳米粒子的电传输和抗菌特性

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.002

Joyshree Maji , Sanjeev Pandey , Soumen Basu

{"title":"The electrical transport and antibacterial properties of Fe doped MgO nanoparticles synthesized by a soft chemical technique","authors":"Joyshree Maji , Sanjeev Pandey , Soumen Basu","doi":"10.1016/j.biotno.2023.12.002","DOIUrl":"10.1016/j.biotno.2023.12.002","url":null,"abstract":"<div>Fe doped MgO nanoparticles were synthesized using a straightforward soft chemical method. We conducted a comprehensive examination of the electrical properties of Fe-doped MgO nanoparticles with a crystalline size range of 7–10 nm. Simultaneously, we explored their antibacterial capabilities. Our findings indicate that an increase in the concentration of Fe-doped MgO correlates with an enhanced bactericidal effect. To gain a deeper understanding of charge transfer processes, the AC conductivity and dielectric characteristics of the samples across various temperatures and frequencies was studied.The antibacterial activity was studied utilising the MIC methodology, the live count (LC) method, and the agar cup technique in addition to the electrical characteristics. After exposure to nanoparticles, we observed the disruption of pathogenic cell walls through transmission electron microscopy (TEM) analysis. These results suggest that Fe-doped MgO nanoparticles hold promise for the development of novel, more effective antibacterial drugs. The ½ MIC for E.coli was found to be 2.75 μg/ml, while for Bacillus sp., it was 1.75 μg/ml when exposed to Fe-doped MgO nanoparticles. This dosage level may find applications in the medical field. However, further investigations are required to assess potential toxicity and long-term environmental and human health effects. If successful in vivo tests follow, Fe-doped MgO nanoparticles could emerge as valuable antibacterial agents.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 150-159"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000144/pdfft?md5=685971f28d934a479bde548ad1c8eba7&pid=1-s2.0-S2665906923000144-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-tuning the cell morphology of Corynebacterium glutamicum via dual-valve regulation for enhanced hyaluronic acid production 通过双阀调节微调谷氨酸棒状杆菌的细胞形态以提高透明质酸产量

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.003

Shuting Yuan , Yukun Zheng , Yan Du , Mingye Song , Claudia Chen Sun , Fangyu Cheng , Huimin Yu

{"title":"Fine-tuning the cell morphology of Corynebacterium glutamicum via dual-valve regulation for enhanced hyaluronic acid production","authors":"Shuting Yuan , Yukun Zheng , Yan Du , Mingye Song , Claudia Chen Sun , Fangyu Cheng , Huimin Yu","doi":"10.1016/j.biotno.2023.12.003","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.003","url":null,"abstract":"<div>Enhanced synthesis of hyaluronic acid (HA) with recombinant Corynebacterium glutamicum as production host was achieved in this work. Hyaluronan synthase (HAS), which is a membrane protein acting as a key enzyme in HA biosynthesis, impacts both HA yield and its molecular weight. Cell morphology, which includes size, shape, and surface area, has a large impact on the expression and activity of HAS. Therefore, deliberate regulation of cell morphology holds the potential to enhance HA production. Here, we constructed three modules, namely the transporter module, the morphology tuning module and the HA synthesis module. The transporter module contains a strong constitutive promoter Ptuf and arabinose transport protein was used to control the maximum amount of inducer entering the cell, thus reducing excessive cell deformation. The morphology tuning module contains an arabinose-inducible weak promoter PBAD and a cell-division-relevant gene was used to sense intracellular inducer concentrations and achieve different degrees of change in cell size. These two modules worked together, described as a dual-valve regulation, to achieve fine-tuning of cell morphology, resulting in a 1.87-fold increase in cell length and a 2.08-fold increase in cell membrane. When combined with the HA synthesis module, the HA titer reached 16.0 g/L, which was 1.6 times the yield reported in the previous morphology-engineered strain. Hence, for the first time, a morphologically engineered strain resulting in both high cell density and HA titer was constructed.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 135-145"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000156/pdfft?md5=38eb27e8aab2ba5af848b5013bd6c72b&pid=1-s2.0-S2665906923000156-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0