Biotechnology Notes最新文献_第5页

Decoding dye degradation: Microbial remediation of textile industry effluents 解码染料降解:纺织工业废水的微生物修复

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.10.001

Soumyajit Das , Lubhan Cherwoo , Ravinder Singh

{"title":"Decoding dye degradation: Microbial remediation of textile industry effluents","authors":"Soumyajit Das , Lubhan Cherwoo , Ravinder Singh","doi":"10.1016/j.biotno.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.10.001","url":null,"abstract":"<div><p>The extensive use of chemical dyes, primarily Azo and anthraquinone dyes, in textiles has resulted in their alarming release into the environment by textile industries. The introduction of heavy metals into these dyes leads to an increase in Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and water toxicity. Conventional physicochemical methods for treating textile effluents are costly and energy-intensive. Here introduction of new strategies is eminent, microbial bioremediation for the biodegradation and detoxification of these hazardous dyes, possesses as an innovative solution for the existing problem, discussed are specific groups of bacteria, fungi, and algae which could be one of the potential decolorizing agents that could replace the majority of other expensive processes in textile wastewater treatment by using enzymes like peroxidase, laccase, and azoreductase. These enzymes catalyzes chemical reactions that break down the dye molecules into less harmful substances. Additionally, novel strategies and advancements to enhance the effectiveness of these microbes and their products are comprehensively discussed.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 64-76"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000065/pdfft?md5=21a407f49cff5a595dbe1b0a2c0c47b0&pid=1-s2.0-S2665906923000065-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol 利用甲醇作为辅助底物生产(R)-1,3-丁二醇的大肠杆菌代谢工程

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.005

Qing Sun , Dehua Liu , Zhen Chen

{"title":"Metabolic engineering of Escherichia coli to utilize methanol as a co-substrate for the production of (R)-1,3-butanediol","authors":"Qing Sun , Dehua Liu , Zhen Chen","doi":"10.1016/j.biotno.2023.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.005","url":null,"abstract":"<div><p>Due to its abundance, cost-effectiveness, and high reducibility, methanol has gained considerable attention in the biomanufacturing industry as a nonfood feedstock for the production of value-added chemicals. The range of chemicals that can be derived from methanol, however, remains constrained and is currently in the concept validation phase. This study aimed to develop and evaluate a hybrid methanol assimilation pathway in <em>Escherichia coli</em> to improve the production of (<em>R</em>)-1,3-butanediol ((<em>R</em>)-1,3-BDO) by utilizing methanol and sugars as co-substrates. By combining the methanol dehydrogenase (MDH) from the prokaryotes with the dihydroxyacetone synthase (DAS) from the eukaryotes, the hybrid pathway facilitates methanol conversion into the central metabolism while generating NADH at the same time. Through pathway optimization and targeted gene deletions, we have successfully developed an <em>E. coli</em> strain capable of producing 5.79 g/L (<em>R</em>)-1,3-BDO in shake flask experiments and 13.71 g/L (<em>R</em>)-1,3-BDO with a yield of 0.35 C-mol/C-mol in batch fermentation using methanol and glucose as co-substrates. Our study also showed the incorporation of <sup>13</sup>C-methanol into cellular intermediates and an increase in NAD(P)H concentration, confirming the role of methanol as a co-substrate and supplier of NADH. In addition, our study also demonstrated the co-utilization of methanol with xylose for the production of (<em>R</em>)-1,3-BDO, expanding the substrate spectrum for sustainable 1,3-BDO production.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 104-111"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000119/pdfft?md5=6b46fabdf4fad0fa9e2396f04c4a5f59&pid=1-s2.0-S2665906923000119-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138564409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae 双质粒相互作用促进缬草烯在酿酒酵母中的积累

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.004

Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li

{"title":"Dual-plasmid interactions stimulate the accumulation of valencene in Saccharomyces cerevisiae","authors":"Chaoyi Zhu, Shengliang Cai, Peiling Liu, Dongying Chen, Jingtao Zhou, Min Zhuo, Shuang Li","doi":"10.1016/j.biotno.2023.12.004","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.12.004","url":null,"abstract":"<div><p>Plasmids are one of the most commonly used basic tools in the construction of microbial cell factories, the use of which individually or in pairs play an important role in the expression of exogenous gene modules. However, little attention has been paid to the interactions of plasmid-plasmid and plasmid-host in the widespread use of the double plasmid system. In this study, we demonstrated that dual-plasmid interactions facilitated to cell growth and product accumulation in <em>Saccharomyces cerevisiae</em>. The strain containing both the expression plasmid pEV (a plasmid carrying the gene encoding valencene synthase) and the assistant plasmid pI (an empty plasmid expressing no extra gene) showed a significant improvement in relative growth rate, biomass and valencene production compared to the strain containing only the pEV plasmid. The transcriptional level analysis revealed an up-regulated expression of specific gene on the expression plasmid pEV stimulated by the assistant plasmid pI in the dual-plasmid interactions. Further investigations demonstrated the essential roles of the promoters of the expression plasmid pEV and the CEN/ARS element of the assistant plasmid pI in the dual-plasmid interactions. Combined with the results of predicted nucleosome occupancy, a response model of interaction based on the key T(n)C and CEN/ARS element was established. Moreover, the transformation order of the two plasmids significantly affected the response effect, implying the dominance of plasmid pI in the dual-plasmid interactions. Our finding first demonstrated that dual plasmids regulate the gene expression through spatial interactions at DNA sequences level, which provides a new perspective for the development of microbial cell factories in future.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 127-134"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000168/pdfft?md5=2d34e1efc0f7c67c058f46ddba2d6840&pid=1-s2.0-S2665906923000168-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2nd symposium on engineering biology and BioFoundry 第二届工程生物学与生物铸造学术研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.004

Yuanli Gao , Chang Dong , Jiazhang Lian, Baojun Wang

引用次数: 0

Synthetic microbes and biocatalyst designs in Thailand 泰国的合成微生物和生物催化剂设计

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.02.003

Duangthip Trisrivirat , Ruchanok Tinikul , Pimchai Chaiyen

引用次数: 1

Synchronous efforts for burgeoning bioeconomy: The 4th international biodesign research conference and international symposium on development and application of modern biotechnology 同步努力，促进蓬勃发展的生物经济：第四届国际生物设计研究大会暨现代生物技术发展与应用国际研讨会

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.12.005

Qiaoning He, Huimin Yu, Shihui Yang

引用次数: 0

Harnessing gut cells for functional insulin production: Strategies and challenges 利用肠道细胞产生功能性胰岛素:策略和挑战

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.11.005

Kelvin Baafi, John C. March

{"title":"Harnessing gut cells for functional insulin production: Strategies and challenges","authors":"Kelvin Baafi, John C. March","doi":"10.1016/j.biotno.2022.11.005","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.11.005","url":null,"abstract":"<div><p>Reprogrammed glucose-responsive, insulin + cells (“<em>β</em>-like”) exhibit the potential to bypass the hurdles of exogenous insulin delivery in treating diabetes mellitus. Current cell-based therapies-transcription factor regulation, biomolecule-mediated enteric signaling, and transgenics - have demonstrated the promise of reprogramming either mature or progenitor gut cells into surrogate “<em>β</em>-like” cells. However, there are predominant challenges impeding the use of gut “<em>β</em>-like” cells as clinical replacements for insulin therapy. Reprogrammed “<em>β</em>-like” gut cells, even those of enteroendocrine origin, mostly do not exhibit glucose – potentiated insulin secretion. Despite the exceptionally low conversion rate of gut cells into surrogate “<em>β</em>-like” cells, the therapeutic quantity of gut “<em>β</em>-like” cells needed for normoglycemia has not even been established. There is also a lingering uncertainty regarding the functionality and bioavailability of gut derived insulin. Herein, we review the strategies, challenges, and opportunities in the generation of functional, reprogrammed “<em>β</em>-like” cells.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 7-13"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-conversion of organic wastes towards polyhydroxyalkanoates 将有机废物生物转化为聚羟基烷酸酯

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.006

Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye

{"title":"Bio-conversion of organic wastes towards polyhydroxyalkanoates","authors":"Zhe-Yi Kuang , Hao Yang , Shi-Wei Shen , Yi-Na Lin , Shu-Wen Sun , Markus Neureiter , Hai-Tao Yue , Jian-Wen Ye","doi":"10.1016/j.biotno.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.006","url":null,"abstract":"<div><p>The bio-manufacturing of products with substantial commercial value, particularly polyhydroxyalkanoates (PHA), using cost-effective carbon sources through microorganisms, has garnered heightened attention from both the scientific community and industry over the past few decades. Opting for industrial PHA production from various organic wastes, spanning industrial, agricultural, municipal, and food-based sources, emerges as a wiser choice. This strategy not only eases the burden of recycling organic waste and curbs environmental pollution but also trims down PHA production costs, rendering these materials more competitive in commercial markets. In addition, PHAs are a family of renewable, environmentally friendly, fully biodegradable and biocompatible polyesters with a multitude of applications. This review provides an overview of recent developments in PHA production from organic wastes. It covers the optimization of diverse metabolic pathways for producing various types of PHA from organic waste sources, pre-treatment and downstream processing for PHA using unrelated organic wastes, and challenges in industrial production of PHA using unrelated organic waste feedstocks and the challenges faced in industrial PHA production from organic wastes, along with potential solutions. Lastly, this study suggests underlying research endeavors aimed at further enhancing of the feasibility of industrial PHA production from organic wastes as an alternative to current petroleum-based plastics in the near future.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 118-126"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000120/pdfft?md5=5995995eb74f3869768728151c55ef72&pid=1-s2.0-S2665906923000120-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138570577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational engineering approaches for establishing insect olfaction reporters in yeast 在酵母中建立昆虫嗅觉报告基因的合理工程方法

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2023.11.002

Emma Elise Hoch-Schneider, Tatyana Saleski, Emil D. Jensen, Michael Krogh Jensen

{"title":"Rational engineering approaches for establishing insect olfaction reporters in yeast","authors":"Emma Elise Hoch-Schneider, Tatyana Saleski, Emil D. Jensen, Michael Krogh Jensen","doi":"10.1016/j.biotno.2023.11.002","DOIUrl":"https://doi.org/10.1016/j.biotno.2023.11.002","url":null,"abstract":"<div><p>Insect olfaction directly impacts insect behavior and thus is an important consideration in the development of smart farming tools and in integrated pest management strategies. Insect olfactory receptors (ORs) have been traditionally studied using <em>Drosophila</em> empty neuron systems or with expression and functionalization in HEK293 cells or <em>Xenopus laevis</em> oocytes<em>.</em> Recently, the yeast <em>Saccharomyces cerevisia</em>e (<em>S. cerevisiae</em>) has emerged as a promising chassis for the functional expression of heterologous seven transmembrane receptors. <em>S. cerevisiae</em> provides a platform for the cheap and high throughput study of these receptors and potential deorphanization. In this study, we explore the foundations of a scalable yeast-based platform for the functional expression of insect olfactory receptors by employing a genetically encoded calcium sensor for quantitative evaluation of fluorescence and optimized experimental parameters for enhanced functionality. While the co-receptor of insect olfactory receptors remains non-functional in our yeast-based system, we thoroughly evaluated various experimental variables and identified future research directions for establishing an OR platform in <em>S. cerevisiae</em>.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 90-99"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665906923000089/pdfft?md5=d694b5387164088876d639cb0d7fc7f5&pid=1-s2.0-S2665906923000089-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138467299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SynBio Africa's story from the grassroots, the present, and the future 非洲合成生物的故事从基层，现在和未来

Biotechnology Notes Pub Date : 2023-01-01 DOI: 10.1016/j.biotno.2022.11.003

Geoffrey Otim , Sandra Matinyi , Erikan Baluku , Ivy S.G. Chimulwa , George P. Magoola , Alex Katumba , Stephen Mukuze , Alex Kyabarongo , Stephen O. Opiyo

{"title":"SynBio Africa's story from the grassroots, the present, and the future","authors":"Geoffrey Otim , Sandra Matinyi , Erikan Baluku , Ivy S.G. Chimulwa , George P. Magoola , Alex Katumba , Stephen Mukuze , Alex Kyabarongo , Stephen O. Opiyo","doi":"10.1016/j.biotno.2022.11.003","DOIUrl":"https://doi.org/10.1016/j.biotno.2022.11.003","url":null,"abstract":"<div><p>SynBio Africa is a forum for researchers, students, citizen scientists, policymakers and the public to convene and develop successful pathways for the propagation of synthetic biology technologies, products, and services throughout Africa. Our vision is to have a healthy, safe, and sustainable world through synthetic biology. In Africa, synthetic biology has the potential to greatly contribute to national development agenda through the following ways: i) by anchoring a sustainable bioeconomy; ii) by helping develop innovative medicines; iii) by reducing pollution, and iv) by increasing crop production to reduce hunger. However, there is little to no information on synthetic biology and its regulatory policies in Africa. Across the continent, scientists, policy makers, researchers and others are still working in silos—only partaking in consultative meetings to try and develop a set of unified policy guidelines. SynBio Africa is therefore proposing to establish the first Center of Excellence in Synthetic Biology in Africa with six themes, namely: research, capacity development, innovation hub, biosafety and biosecurity, and bioinformatics and data science, and one-health. Accordingly, SynBio Africa will work with collaborators from government and non-governmental organizations, the public and private sectors, and educational institutions from Uganda, Africa, and around the world to implement these six themes.</p></div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"4 ","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49716401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1