Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides最新文献

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Immunochemical studies of the homogeneity of bovine submaxillary mucins 牛颌下黏液同质性的免疫化学研究
Martin I. Horowitz, Leonore Martinez, V.L.N. Murty
{"title":"Immunochemical studies of the homogeneity of bovine submaxillary mucins","authors":"Martin I. Horowitz,&nbsp;Leonore Martinez,&nbsp;V.L.N. Murty","doi":"10.1016/0926-6526(64)90008-4","DOIUrl":"10.1016/0926-6526(64)90008-4","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Immunochemical methods were used to study the homogeneity and immunological relationships of bovine submaxillary mucin preparations isolated according to the methods described by <span>Gottschalk</span><sup>1</sup> and by <span>Pigman</span><sup>2</sup> and their collaborators and disgnated BSM-G (“described by <span>Gottschalk</span>” and BSM-G (“described by <span>Pigman</span>”), respectively.</p></span></li><li><span>2.</span><span><p>2. Antisera suitable for immunodiffusion and immunoelectrophoresis were obtained after intravenous and subcutaneous (with Freunds's adjuvant) injections into rabbits of submaxillary gland extracts and mucins. Antibodies directed against the principal mucin components were produced after injection of BSM-P; this was demonstrated by the quantitative precipitation of sialic acid upon addition of BSM-P to anti-BSM-P.</p></span></li><li><span>3.</span><span><p>3. Neither BSM-G nor BSM-P were homogenous by precipitin-curve analysis. Immunoelectrophoresis showed that BSM-G preparations contained from four to nine components, three of which were glycoproteins. BSM-P did not contain extraneous proteins, but it consisted of at least two glycoproteins. Two of the glycoproteins in BSM-G formed confluent arcs with BSM-P.</p></span></li><li><span>4.</span><span><p>4. Further fractionation<sup>2</sup> BSM-G afforded a fraction which was indistinguishable from BSM-P and which corresponds to approx. 60% of the dry weight of BSM-G.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 305-317"},"PeriodicalIF":0.0,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90008-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23785829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Reduction of ovomucin by mercaptoethanol 巯基乙醇还原卵磷脂
D.S. Robinson, J.B. Monsey
{"title":"Reduction of ovomucin by mercaptoethanol","authors":"D.S. Robinson,&nbsp;J.B. Monsey","doi":"10.1016/0926-6526(64)90021-7","DOIUrl":"10.1016/0926-6526(64)90021-7","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 368-370"},"PeriodicalIF":0.0,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90021-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23789291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Variability of keratan sulfate isolated from beef cornea 从牛肉角膜分离硫酸角蛋白的变异
Bernard Wortman
{"title":"Variability of keratan sulfate isolated from beef cornea","authors":"Bernard Wortman","doi":"10.1016/0926-6526(64)90006-0","DOIUrl":"10.1016/0926-6526(64)90006-0","url":null,"abstract":"<div><p>Glycosaminoglycans were isolated from beef cornea and further fractionated on the basis of ethanol solubility. Variability of keratan sulfate was found. The forms differ in reducing activity, sulfur content, infrared spectra and specific optical rotation. Keratan sulfotransferase (3′-phosphoadenylylsulfate: keratan sulfotransferase) activity is low but detectable in beef cornea-epithelial extract. Keratan sulfate with the lower sulfate content acts as a sulfate acceptor while the other does not.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 288-295"},"PeriodicalIF":0.0,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23785827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
A rapid procedure for the preparation of small quantities of uridine diphosphate-N-[14C]acetylhexosamine 快速制备少量尿苷二磷酸- n -[14C]乙酰己糖胺的方法
Paul J. O'Brien, Elizabeth F. Neufeld
{"title":"A rapid procedure for the preparation of small quantities of uridine diphosphate-N-[14C]acetylhexosamine","authors":"Paul J. O'Brien,&nbsp;Elizabeth F. Neufeld","doi":"10.1016/0926-6526(64)90015-1","DOIUrl":"10.1016/0926-6526(64)90015-1","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 352-354"},"PeriodicalIF":0.0,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90015-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23785836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Cell walls of three strains of mycobacteria (Mycobacterium phlei, Mycobacterium fortuitum and Mycobacterium kansasii) 3株分枝杆菌(静脉分枝杆菌、幸运分枝杆菌和堪萨斯分枝杆菌)细胞壁的测定
Hermine de Wijs , Pierre Jollès
{"title":"Cell walls of three strains of mycobacteria (Mycobacterium phlei, Mycobacterium fortuitum and Mycobacterium kansasii)","authors":"Hermine de Wijs ,&nbsp;Pierre Jollès","doi":"10.1016/0926-6526(64)90010-2","DOIUrl":"10.1016/0926-6526(64)90010-2","url":null,"abstract":"<div><p>The isolation of the cell walls of three mycobacterial strains is described and their chemical compositions have been determined. The cell-wall preparations were purified by treatment with trypsin (EC 3.4.4.4), chymotrypsin (EC 3.4.4.5) and pepsin (EC 3.4.4.1). Digestion with lysozymes (EC 3.2.1.17) from hen's egg-white, duck's egg-white and human milk resulted in the liberation of substances which reacted with reagents specific for amino sugars.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 326-332"},"PeriodicalIF":0.0,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90010-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83638149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Studies on the biochemistry of heart valves II. The effect of aging and anti-inflammatory drugs on the synthesis of glucosamine 6-phosphate and phosphoadenosine phosphosulfate by bovine heart valves 心脏瓣膜的生物化学研究2。衰老和抗炎药物对牛心脏瓣膜合成6-磷酸氨基葡萄糖和硫酸磷酸腺苷的影响
Bernard Jacobson , Harry Boström
{"title":"Studies on the biochemistry of heart valves II. The effect of aging and anti-inflammatory drugs on the synthesis of glucosamine 6-phosphate and phosphoadenosine phosphosulfate by bovine heart valves","authors":"Bernard Jacobson ,&nbsp;Harry Boström","doi":"10.1016/0926-6526(64)90031-X","DOIUrl":"10.1016/0926-6526(64)90031-X","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. <span>l</span>-Glutamine-<span>d</span>-fructose 6-phosphate aminotransferase (EC 2.6.1.16) has been isolated from liquid nitrogen-powdered aortic and pulmonary-valves of calves and cows.</p></span></li><li><span>2.</span><span><p>2. Young valves show greater enzymatic activity than older, while, in calves, the aortic-valve aminotransferase is more active than the pulmonary-valve enzyme.</p></span></li><li><span>3.</span><span><p>3. Sodium salicylate produces a marked inhibition of the aortic and pulmonary-valve aminotransferase, while its isomer, <em>p</em>-hydroxybenzoic acid has a much less inhibitory effect. Chloroquine diphosphate causes a marked inhibition only at high concentration.</p></span></li><li><span>4.</span><span><p>4. The aortic and pulmonary-valve 3′-phosphoadenosine-5′-phosphosulphate-synthesizing systems have been isolated and have been found to be unaffected by the drugs studied.</p></span></li><li><span>5.</span><span><p>5. The possibility of the enzymatic synthesis of glucosamine 6-phosphate being one control point for the action of some anti-inflammatory drugs is discussed.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 152-164"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90031-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Blood-group-substance activity of bovine submaxillary mucin I. J activity of bovine submaxillary mucin and properties of bovine submaxillary mucin glycopeptides 牛颌下粘蛋白的血群物质活性ⅰ。牛颌下粘蛋白的活性和牛颌下粘蛋白糖肽的性质
Martin I. Horowitz, Yohichi Hashimoto, Ward Pigman
{"title":"Blood-group-substance activity of bovine submaxillary mucin I. J activity of bovine submaxillary mucin and properties of bovine submaxillary mucin glycopeptides","authors":"Martin I. Horowitz,&nbsp;Yohichi Hashimoto,&nbsp;Ward Pigman","doi":"10.1016/0926-6526(64)90037-0","DOIUrl":"10.1016/0926-6526(64)90037-0","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The presence of blood-group-substance activity in bovine saliva prompted investigation of the immunologic behavior of bovine submaxillary mucin and of glycopeptides obtained by proteolytic digestion of the mucin.</p></span></li><li><span>2.</span><span><p>2. Purified preparations of bovine submaxillary mucin inhibited hemolysis in the bovine J-anti-J system. Removal of sialic acid from the mucin by mild acid hydrolysis increased the potency of the preparation. Periodate oxidation of sialic acid free-bovine submaxillary mucin destroyed the blood-group J activity.</p></span></li><li><span>3.</span><span><p>3. Bovine submaxillary mucin exhibited a high degree of specificity. it did not inhibit other bovine blood group systems, and it was inactive in human B, M, N and I, i systems; bovine submaxillary mucin had moderate inhibition in the A and O (H) systems.</p></span></li><li><span>4.</span><span><p>4. Bovine submaxillary mucin was digested by pronase and papain (EC 3.4.4.10), and the glycopeptides were fractionated through Sephadex G-50. Glycopeptide fractions which contained the greatest amounts of hexosamine, galactose, and fucose relative to sialic acid were the best glycopeptide inhibitors of J hemolysis.</p></span></li><li><span>5.</span><span><p>5. Bovine submaxillary mucin cross-reacted with antiserum to Type XIV pneumococcal polysaccharide only after removal of sialic acid from the mucin. Possibly some <span>d</span>-galactosyl residues were exposed by the removal of sialic acid from bovine submaxillary mucin.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 209-217"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Études sur la structure d'une α1-glycoprotéine α1-糖蛋白结构的研究
Roland Bourrillon , René Got , Denise Meyer
{"title":"Études sur la structure d'une α1-glycoprotéine","authors":"Roland Bourrillon ,&nbsp;René Got ,&nbsp;Denise Meyer","doi":"10.1016/0926-6526(64)90033-3","DOIUrl":"10.1016/0926-6526(64)90033-3","url":null,"abstract":"<div><p>A sialic acid-free glycopeptide preparation was isolated from a proteolytic digest of <em>α</em><sub>1</sub>-glycoprotein (pleuromucoid). By paper electrophoresis at pH 6.4, this fraction showed four zones of same carbohydrate composition but of different amino acid composition and sequence.</p><p>This preparation was submitted to digestion with response, resulting in the release of some free or combined amino acids. After purification by gel filtration on Sephadex G 25, a new glycopeptide was obtained; only a limited number of amino acids were present: aspartic and glutamic acids, threonine, proline, glycine and lysine. By paper electrophoresis at pH 6.4, three zones were identified; their amino acid composition and sequence were determined.</p><p>The structure of the three glycopeptide was found different. Similar results were obtained with orosomucoid.</p><p>The present evidence suggests that the diverse glycopeptide chains of the <em>α</em><sub>1</sub>-glycopeptide don't have all the same consitutition.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 178-188"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90033-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83036688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
On the formation of mucin in tracheal mucosa of the rat 大鼠气管粘膜粘蛋白形成的研究
P. Formijne, J.B. Van der Schoot, Ineke De Nie
{"title":"On the formation of mucin in tracheal mucosa of the rat","authors":"P. Formijne,&nbsp;J.B. Van der Schoot,&nbsp;Ineke De Nie","doi":"10.1016/0926-6526(64)90042-4","DOIUrl":"10.1016/0926-6526(64)90042-4","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 239-241"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90042-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Parallel labelling of non-dialyzable components of rabbit urine following 75SeO4 and 35SO4 injection 注射75SeO4和35SO4后兔尿中不可透析成分的平行标记
John T. Galambos, Irving Green
{"title":"Parallel labelling of non-dialyzable components of rabbit urine following 75SeO4 and 35SO4 injection","authors":"John T. Galambos,&nbsp;Irving Green","doi":"10.1016/0926-6526(64)90036-9","DOIUrl":"10.1016/0926-6526(64)90036-9","url":null,"abstract":"<div><p>Following the intravenous administration of Na<sub>2</sub><sup>75</sup>SeO<sub>4</sub> and Na<sub>2</sub><sup>35</sup>SO<sub>4</sub> in rabbits, the non-dialyzable solids of urine were labelled with <sup>35</sup>S and <sup>75</sup>Se in a parallel manner both on BaCO<sub>3</sub>-fractionated specimens and when chondroitin sulfate A and C were isolated by glass-paper chromatography. <sup>75</sup>Se-labelled selenate may be a useful adjunct to the tools currently available for the metabolic studies of sulfomucopolysaccharides.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 204-208"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90036-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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