3株分枝杆菌(静脉分枝杆菌、幸运分枝杆菌和堪萨斯分枝杆菌)细胞壁的测定

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-11-01 DOI:10.1016/0926-6526(64)90010-2

Hermine de Wijs , Pierre Jollès

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引用次数: 21

摘要

本文描述了三种分枝杆菌菌株细胞壁的分离，并测定了它们的化学成分。用胰蛋白酶(EC 3.4.4.4)、胰凝乳蛋白酶(EC 3.4.4.5)和胃蛋白酶(EC 3.4.4.1)纯化细胞壁。用溶菌酶(EC 3.2.1.17)对母鸡蛋清、鸭蛋清和人乳进行消化，释放出与氨基糖特异性试剂反应的物质。

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Cell walls of three strains of mycobacteria (Mycobacterium phlei, Mycobacterium fortuitum and Mycobacterium kansasii)

The isolation of the cell walls of three mycobacterial strains is described and their chemical compositions have been determined. The cell-wall preparations were purified by treatment with trypsin (EC 3.4.4.4), chymotrypsin (EC 3.4.4.5) and pepsin (EC 3.4.4.1). Digestion with lysozymes (EC 3.2.1.17) from hen's egg-white, duck's egg-white and human milk resulted in the liberation of substances which reacted with reagents specific for amino sugars.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides

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