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Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides最新文献

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Studies of the spore coats of fungi I. Isolation and composition of the spore coats of Aspergillus oryzae 真菌孢子被膜的研究I.米曲霉孢子被膜的分离与组成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90035-7
Koki Horikoshi, Shigeji Iida
{"title":"Studies of the spore coats of fungi I. Isolation and composition of the spore coats of Aspergillus oryzae","authors":"Koki Horikoshi,&nbsp;Shigeji Iida","doi":"10.1016/0926-6526(64)90035-7","DOIUrl":"10.1016/0926-6526(64)90035-7","url":null,"abstract":"<div><p>Spore coats of <em>Aspergillus oryzae</em> have been mechanically isolated. They exhibited a complex chemical composition consisting of polysaccharide (mannose, glucose, galactose and glucosamine), phosphate, protein and nucleic acid. Spore coats were partially hydrolyzed by the lytic enzyme from <em>Bacillus circulans</em>, and glucose, laminaribiose and other unknown sugars were detected in the hydrolyzate. No qualitative differences were found between the cell walls and the spore coats. The major quantitative difference was in protein content, which was higher in the spore coats.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 197-203"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90035-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 46
Characterization of trichloroacetic acid-soluble plasma protein and its variations in renal insufficiency 三氯乙酸溶性血浆蛋白的特征及其在肾功能不全中的变化
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90040-0
Kurt H. Stenzel , Glenn D. Lubash , Peter F. Davison , Albert L. Rubin
{"title":"Characterization of trichloroacetic acid-soluble plasma protein and its variations in renal insufficiency","authors":"Kurt H. Stenzel ,&nbsp;Glenn D. Lubash ,&nbsp;Peter F. Davison ,&nbsp;Albert L. Rubin","doi":"10.1016/0926-6526(64)90040-0","DOIUrl":"10.1016/0926-6526(64)90040-0","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Protein material in 5% trichloroacetic acid extracts of human plasma appears physically homogeneous and closely resembles <em>α</em><sub>1</sub>-acid glycoprotein (orosomucoid). Molecular weight is estimated at 41000 (±2000), <em>E</em><sub>1%,icm<sup>278</sup></sub> is 7.7, and the protein has a high carbohydrate and sialic acid content.</p></span></li><li><span>2.</span><span><p>2. Concentrations of this protein are markedly elevated in renal insufficiency.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 231-236"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90040-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Acid mucopolysaccharides in aortic tissue of the dog 狗主动脉组织中的酸性粘多糖
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90030-8
Otakar V. Sirek , Sara Schiller , Albert Dorfman
{"title":"Acid mucopolysaccharides in aortic tissue of the dog","authors":"Otakar V. Sirek ,&nbsp;Sara Schiller ,&nbsp;Albert Dorfman","doi":"10.1016/0926-6526(64)90030-8","DOIUrl":"10.1016/0926-6526(64)90030-8","url":null,"abstract":"<div><p>Thoracic and abdominal aortas from dogs were analyzed for their mucopolysaccharide content by a modification of existing methods. Acid mucopolysaccharides represented approx. 0.7% of the dry weight. The major components were chondroitinsulfuric acid A and C, with lesser amounts of heparitin sulfate and negligible amounts of hyaluronic acid and chondroitinsulfuric acid B.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 148-151"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90030-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Modulation of the intrinsic viscosity of hyaluronic acid formed by human “fibroblasts” in vitro: The effects of hydrocortisone and colchicine 人“成纤维细胞”在体外形成的透明质酸固有粘度的调节:氢化可的松和秋水仙碱的作用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90032-1
C. William Castor, Robert K. Prince
{"title":"Modulation of the intrinsic viscosity of hyaluronic acid formed by human “fibroblasts” in vitro: The effects of hydrocortisone and colchicine","authors":"C. William Castor,&nbsp;Robert K. Prince","doi":"10.1016/0926-6526(64)90032-1","DOIUrl":"10.1016/0926-6526(64)90032-1","url":null,"abstract":"<div><p>Data are presented to show that the intrinsic viscosity of hyaluronic acid elaborated into the culture medium supportinf human connective-tissue cells <em>in vitro</em> is modulated in a systematic manner by the cells. Intrinsic viscosity is reduced during rapid cell division and by hydrocortisone treatment. Conversely, the initial lag phase of culture growth and colchicine treatment are attended by formation of hyaluronic acid with a high intrinsic viscosity.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 165-177"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90032-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Incorporation of sulfate and the synthesis of collagen by cultures of embryonic chondrocytes 胚胎软骨细胞培养中硫酸盐的掺入和胶原的合成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90034-5
Darwin J. Prockop, Olive Pettengill, Howard Holtzer
{"title":"Incorporation of sulfate and the synthesis of collagen by cultures of embryonic chondrocytes","authors":"Darwin J. Prockop,&nbsp;Olive Pettengill,&nbsp;Howard Holtzer","doi":"10.1016/0926-6526(64)90034-5","DOIUrl":"10.1016/0926-6526(64)90034-5","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. In order to determine whether the collagen found in cartilage is synthesized by the same cells which synthesize chondroitin sulfate, <sup>3</sup>H or <sup>14</sup>C-labeled proline was administered to pure cultures of freshly liberated chondrocytes, and the synthesis of collagen was followed by the appearance of labeled hydroxyproline. The results indicated that the chondrocytes synthesized collagen</p></span></li><li><span>2.</span><span><p>2. Double-label experiments with the chondrocytes indicated that the collagen synthesis occurred concurrently with sulfate incorporation.</p></span></li><li><span>3.</span><span><p>3. The incorporation of [<sup>35</sup>S]sulfate and synthesis of labeled hydroxyproline was also examined in the progeny of chondrocytes which were grown on fibrin clots for several generations. These “de-differentiated chondrocytes” did not incorporate [<sup>35</sup>S]-sulfate or synthesize labeled hydroxyproline even though the rates of labeled proline incorporation were similar to those observed with freshly liberated chondrocytes.</p></span></li><li><span>4.</span><span><p>4. The failure of de-differentiated chondrocytes to incorporate sulfate and to synthesize collagen suggests that the pathways for the synthesis of sulfated mucopolysaccharides and collagen may be closely interrelated.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 189-196"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 38
Incorporation of [14C]glucose into lactose and neuramin-lactose by rat mammary glands [14C]葡萄糖在大鼠乳腺中与乳糖和神经氨酸乳糖的结合
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90039-4
Raul Carubelli , Bariah Taha , Raul E. Trucco , Ranwel Caputto
{"title":"Incorporation of [14C]glucose into lactose and neuramin-lactose by rat mammary glands","authors":"Raul Carubelli ,&nbsp;Bariah Taha ,&nbsp;Raul E. Trucco ,&nbsp;Ranwel Caputto","doi":"10.1016/0926-6526(64)90039-4","DOIUrl":"10.1016/0926-6526(64)90039-4","url":null,"abstract":"<div><p>Incubation of rat-mammary-gland slices in a physiological solution containing [<sup>14</sup>C]glucose disclosed incorporation of radioactivity into both lactose and neuraminlactose. After 1 min incubation the labeling was restricted to the glucose moiety of these molecules while administering the glucose <em>in vivo</em> resulted in labeling of all components of these molecules after 3 h.</p><p>The specific activities of free lactose and lactose from neuramin-lactose are of similar order of magnitude in all experiments and, although these findings are compatible with the currently known biosynthetic pathways of these substances, the possibility of an alternate pathway involving the action of neuraminidase is suggested in connection with the rapid equilibration of the specific activities of these substances.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 224-230"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90039-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Biochemistry of the cell wall of Nitrosomonas europaea 欧洲亚硝化单胞菌细胞壁的生物化学研究
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90041-2
Ronald F. Lewis
{"title":"Biochemistry of the cell wall of Nitrosomonas europaea","authors":"Ronald F. Lewis","doi":"10.1016/0926-6526(64)90041-2","DOIUrl":"10.1016/0926-6526(64)90041-2","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 237-239"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90041-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The interaction between polysaccharides and other macromolecules VII. The effects of various polymers on the sedimentation rates of serum albumin and α-crystallin 多糖与其他大分子的相互作用7。不同聚合物对血清白蛋白和α-结晶蛋白沉降速率的影响
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90029-1
Torvard C. Laurent, Håkan Persson
{"title":"The interaction between polysaccharides and other macromolecules VII. The effects of various polymers on the sedimentation rates of serum albumin and α-crystallin","authors":"Torvard C. Laurent,&nbsp;Håkan Persson","doi":"10.1016/0926-6526(64)90029-1","DOIUrl":"10.1016/0926-6526(64)90029-1","url":null,"abstract":"<div><p>The sedimentation behaviour of two globular proteins of different size has been studied in solutions of polymers of varied structure. That the movement of the larger protein was in all instances retarded to a greater extent than that of the smaller indicated that the polymers investigated had a sieving effect. The ability of a polymer to inhibit the migration of another substance was demonstrated to increase with its degree of polymerization and decrease with branching. Charged substituents in a polymer were found to augment materially its effectiveness in restricting transport.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 141-147"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90029-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40871783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
The effect of neuraminidase on the properties of γ-glutamyl transpeptidase 神经氨酸酶对γ-谷氨酰转肽酶性质的影响
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90038-2
A. Szewczuk, G.E. Connell
{"title":"The effect of neuraminidase on the properties of γ-glutamyl transpeptidase","authors":"A. Szewczuk,&nbsp;G.E. Connell","doi":"10.1016/0926-6526(64)90038-2","DOIUrl":"10.1016/0926-6526(64)90038-2","url":null,"abstract":"<div><p>γ-Glutamyl transpeptidase preparations contain bound neuraminic acids which can be removed by treatment with neuraminidase (EC 3.2.1.18). The products of enzymatic digestion have been identified as <em>N</em>-acetyl- and <em>N</em>-glycolylneuraminic acids by paper chromatography. The activity of the enzyme is not affected by digestion with neuraminidase. The stability in acid solution is increased, and the electrophoretic behaviour and the sedimentation patterns are altered after treatment with the enzyme.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 218-223"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90038-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Galacturonic acid in hydrolyzates of defatted human brain 脱脂人脑水解物中的半乳糖醛酸
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI: 10.1016/0926-6526(64)90043-6
Z. Stary, A. Wardi, D. Turner
{"title":"Galacturonic acid in hydrolyzates of defatted human brain","authors":"Z. Stary,&nbsp;A. Wardi,&nbsp;D. Turner","doi":"10.1016/0926-6526(64)90043-6","DOIUrl":"10.1016/0926-6526(64)90043-6","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 242-244"},"PeriodicalIF":0.0,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40764244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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