Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides最新文献_第5页

Enzymic degradation of uridine diphosphoacetylglucosamine 尿苷二磷酸乙酰氨基葡萄糖的酶降解

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90053-9

T.N. Pattabiraman, T.N. Sekhara Varma, B.K. Bachhawat

引用次数: 21

Macromolecular properties and biological activity of heparin III. Paper electrophoretic studies of histamine binding 肝素III的大分子性质及生物活性。组胺结合的纸电泳研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90058-8

Grant H. Barlow

引用次数: 11

The purification and characterization of a new active immunizing polysaccharide prepared from Staphylococcus aureus 一种新型金黄色葡萄球菌活性免疫多糖的纯化及特性研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90048-5

Theodore H. Haskell, Stephen Hanessian

引用次数: 18

Enzymic activities associated with the induction of chondrogenesis in vitro 体外诱导软骨形成的酶活性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90054-0

Mary Catherine Glick , James W. Lash, John W. Madden

{"title":"Enzymic activities associated with the induction of chondrogenesis in vitro","authors":"Mary Catherine Glick , James W. Lash, John W. Madden","doi":"10.1016/0926-6526(64)90054-0","DOIUrl":"10.1016/0926-6526(64)90054-0","url":null,"abstract":"<div>Chick embryo somites, Stages 16–17, when grown in vitro with embryonic notochord or spinal cord, form cartilage, in contrast to somites grown in the absence of notochord or spinal cord.In a study of this system of embryonic induction, cell-free extracts of both types of cultures were examined for the presence of ATP-sulfurylase (ATP:sulfate adenylyl-transferase, EC 2.7.7.4) and adenosine-5′-phosphosulfate kinase (ATP:adenylylsulfate 3′-phosphotransferase, EC 2.7.1.25). Both enzymes were demonstrated in the somites stimulated with notochord by the ability of the enzymic preparations to form 3′-phosphoadenosine-5′-phosphosulfate from ATP, Mg2+ and 35SO42-. Formation of 3′-phosphoadenosine-5′-phosphosulfate was not detected in somites cultured without notochord.3′-Phosphoadenosine-5′-phosphosulfate generated by extracts of cartilage-forming somites was characterized by electrophoretic and chromatographic behavior, lability to mild acid, ability to serve as substrate with rabbit-liver sulfokinase (3′-phosphoadenylylsulfate: phenol sulfotransferase, EC 2.8.2.1), and the formation of adenosine 5′-phosphosulfate after incubation with alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1).Cell-free extracts were also prepared from tissues which were isolated from the embryo but not cultured. These extracts of somites, notochords and spinal cords, and mesonephri had no demonstrable activity for ATP-sulfurylase and adenosine-5′-phosphosulfate kinase. Cell-free preparations from the whole trunk, however, showed considerable activity for these enzymes.</div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 84-92"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90054-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Incorporation of radioactive glucosamine into the serum proteins of intact rats and rabbits 放射性葡萄糖胺掺入完整大鼠和家兔血清蛋白

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90055-2

M.R. Shetlar, Jerry C. Capps, Danny L. Hern

{"title":"Incorporation of radioactive glucosamine into the serum proteins of intact rats and rabbits","authors":"M.R. Shetlar, Jerry C. Capps, Danny L. Hern","doi":"10.1016/0926-6526(64)90055-2","DOIUrl":"10.1016/0926-6526(64)90055-2","url":null,"abstract":"<div>d-[i-14C]Glucosamine was administered to rats and rabbits by intraperitoneal injection in order to study the incorporation of hexosamines into the glycoproteins of liver and serum. Maximum radioactivity of the fraction soluble in trichloroacetic acid was found in rat livers within 30 min after injection and in rabbit livers within 1 h. Highest values for radioactivity in the trichloroacetic acid-insoluble fraction were found at 3 h for the rat. Maximum protein-bound radioactivity in the serum was found at 3 h for the rat and at 7 h for the rabbit. Efficient incorporation into serum proteins was found in both species, being 24 % and 28 % of the administered dose for rats and rabbits, respectively, at the maximum level of radioactivity. About 22 % of the bound radioactivity of serum was found associated with sialic acid in the rat, most of the remaining 78 % was associated with glucosamine, and only negligible amounts of radioactivity were found in the hexoses. Liver glycogen was not radioactive. Glucosamine was isolated from serum protein hydrolysates with the radioactivity in the number-one position. Therefore, it appears that parenterally administered glucosamine is incorporated into liver and tissue glycoproteins without degradation. The value of [i-14C]glucosamine as a tool for further studies of glycoprotein metabolism is indicated.</div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 93-101"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90055-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Alkaline degradation of the phosphomucopolysaccharide from cell walls of Bacillus megaterium KM 巨芽孢杆菌细胞壁磷多聚糖的碱性降解

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90063-1

J.M. Ghuysen

引用次数: 10

Acid mucopolysaccharide sulphokinases in human serum 人血清中的酸性粘多糖磺酸激酶

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90061-8

J.B. Adams

引用次数: 21

N-glycolyl-8-O-methylneuraminic acid A new form of sialic acid in the starfish Asterias forbesi n -羟基乙酸-8- o -甲基神经氨酸:海星中唾液酸的一种新形式

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90062-X

Leonard Warren

引用次数: 39

Isolation of 2-amino-2-deoxy-d-galactoside disaccharide from chondroitin sulfate C 硫酸软骨素中2-氨基-2-脱氧-d-半乳糖糖苷双糖的分离

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90046-1

Konoshin Onodera, Tohru Komano, Shigehiro Hirano

{"title":"Isolation of 2-amino-2-deoxy-d-galactoside disaccharide from chondroitin sulfate C","authors":"Konoshin Onodera, Tohru Komano, Shigehiro Hirano","doi":"10.1016/0926-6526(64)90046-1","DOIUrl":"10.1016/0926-6526(64)90046-1","url":null,"abstract":"<div>Purified chondroitin sulfate prepared from shark cartilage (chondroitin sulfate C) was desulfated and esterified with methanolic HCl. Chondroitin methyl ester (II) was reduced with NaBH4 to give carboxyl-reduced chondroitin (III), which was submitted to hydrazinolysis to remove the acetyl group of the 2-acetamido-2-deoxy-d-galactopyranose moiety. The N-deacetylated carboxyl-reduced chondroitin (IV) was dyrolyzed with HCl and the hydrolyzate was passed down a Dowex-50 (H+) column. A disaccharide (V) and 2-amino-2-deoxy-d-galactopyranose adsorbed on the column were eluted with dilute HCl. Both the substances were N-acetylated and separated effectively by means of a cellulose powder column (Solvent B), and a novel disaccharide, <math><msub><mi></mi><mn>4</mn></msub><mtext>-O-(β-2-</mtext><mtext>acetamido-2-deoxy</mtext><mtext>-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>galactopyranosyl</mtext><mtext>)-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>glucopyranose</mtext></math> (VII), was isolated. The reducing end of the isolated disaccharide was reduced with NaBH4 to give <math><msub><mi></mi><mn>4</mn></msub><mtext>-O-(β-2-</mtext><mtext>acetamido-2-deoxy</mtext><mtext>-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>galactopyranosyl</mtext><mtext>)-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>glucitol</mtext></math> (VIII). Acid hydrolysis of this substance gave d-glucitol (IX) and 2-amino-2-deoxy-d-galactopyranose (X), which were characterized by infrared spectra and by paper chromatography. The structures of these substances (VII, VIII) were confirmed by means of periodate oxidation.</div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 20-26"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90046-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Differences in glycosaminoglycans derived from chick embryo chondrocytes grown in vitro and in vivo 鸡胚软骨细胞体外和体内提取的糖胺聚糖的差异

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90051-5

Mary Catherine Glick , Frank E. Stockdale

{"title":"Differences in glycosaminoglycans derived from chick embryo chondrocytes grown in vitro and in vivo","authors":"Mary Catherine Glick , Frank E. Stockdale","doi":"10.1016/0926-6526(64)90051-5","DOIUrl":"10.1016/0926-6526(64)90051-5","url":null,"abstract":"<div>Vertebral chondrocytes from 10-day-old chick embryos were examined after growth for five days in vitro and in vivo. The chondrocytes, including the hyaline matrix, from both methods of growth were extracted for glycosaminoglycuronoglycans and the partially purified polymers were analyzed for hexosamines. It was found that the chondrocytes, when removed from their cartilage matrix and grown in vitro, synthesized sulfated glycosaminoglycans which migrated in electrophoresis as chondroitin sulfates. The partially purified glycosaminoglycans were not, however, chondroitin sulfates since glucosamine was characterized as the major hexosamine. Neither galactose nor glucuronic acid were demonstrated as part of the glucosamine-containing polymer. The polymer was produced by the cells grown in vitro regardless of age or original location, dorsal or ventral, in the vertebral column. In contrast, chondrocytes which were grown in vivo for 10 or 15 days synthesized predominately a polymer which had characteristics of chondroitin 4- or 6-sulfate.</div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 61-68"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90051-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11