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Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides最新文献

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The determination of small quantities of isomeric chondroitin sulfates 硫酸异构体软骨素的少量测定
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90065-5
Marian Schmidt, Antoni Dmochowski
{"title":"The determination of small quantities of isomeric chondroitin sulfates","authors":"Marian Schmidt, Antoni Dmochowski","doi":"10.1016/0926-6526(64)90065-5","DOIUrl":"10.1016/0926-6526(64)90065-5","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 137-140"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90065-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23739782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
The effect of methylprednisolone on mucopolysaccharides of rabbit vitreous humor and costal cartilage 甲基强的松龙对兔玻璃体和肋软骨粘多糖的影响
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90056-4
David Kaplan, Barry Fisher
{"title":"The effect of methylprednisolone on mucopolysaccharides of rabbit vitreous humor and costal cartilage","authors":"David Kaplan,&nbsp;Barry Fisher","doi":"10.1016/0926-6526(64)90056-4","DOIUrl":"10.1016/0926-6526(64)90056-4","url":null,"abstract":"<div><p>Soluble methylprednisolone, given to rabbits in a dose of 3 mg in 1 ml intramuscularly per day causes (1) an increase in the concentration of mucopolysaccharide uronic acid in the vitreous humor compared with concentrations in a saline-treated control group, and (2) an increase in the absolute amount of mucopolysaccharide glucosamine in the costal cartilage of the same animals with an accompanying increased concentration of neutral sugar as measured by the anthrone reaction.</p><p>On the basis of data obtained, it is postulated that adrenal corticosteroids produce both an increase of hyaluronate in the vitreous humor and an increase in keratosulfate in costal cartilage.</p><p>It is thought that the most likely explanation of these phenomena is that these steroid hormones in small doses, regardless of whether or not they inhibit both synthesis and breakdown, cause an overall inhibition of degradation of these glucosamine-containing substances.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 102-112"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90056-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23734443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Structural studies of heparin II. Exchangeable anions 肝素II的结构研究。可交换的离子
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90059-X
J.R. Helbert , M.A. Marini
{"title":"Structural studies of heparin II. Exchangeable anions","authors":"J.R. Helbert ,&nbsp;M.A. Marini","doi":"10.1016/0926-6526(64)90059-X","DOIUrl":"10.1016/0926-6526(64)90059-X","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 122-124"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90059-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Paper chromatography of galacturonic acids to determine polygalacturonase activity 半乳糖醛酸纸层析法测定聚半乳糖醛酸酶活性
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90060-6
Robert J. Young , Malcolm E. Corden
{"title":"Paper chromatography of galacturonic acids to determine polygalacturonase activity","authors":"Robert J. Young ,&nbsp;Malcolm E. Corden","doi":"10.1016/0926-6526(64)90060-6","DOIUrl":"10.1016/0926-6526(64)90060-6","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 124-126"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90060-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Some properties and the structure of a glucosamine-aspartic fragment from ovalbumin 卵清蛋白中氨基-天冬氨酸片段的一些性质和结构
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90052-7
V.P. Bogdanov, E.D. Kaverzneva, A.P. Andreyeva
{"title":"Some properties and the structure of a glucosamine-aspartic fragment from ovalbumin","authors":"V.P. Bogdanov,&nbsp;E.D. Kaverzneva,&nbsp;A.P. Andreyeva","doi":"10.1016/0926-6526(64)90052-7","DOIUrl":"10.1016/0926-6526(64)90052-7","url":null,"abstract":"<div><p>A preparative method has been developed for the isolation of a fraction containing both glucosamine and aspartic acid by hydrolysis of the previously described polysaccharide-aspartic acid fraction from egg albumin.</p><p>It was confirmed, that the polysaccharide group is bound to the protein through a terminal glucosamine residue.</p><p>The quantitative composition and the chemical and physical properties of the fragment agree with the structure of a 2-acetamido-1-β (<span>l</span>-β-aspartamido)-1,2-deoxy-<span>d</span>-glucose.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 69-73"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90052-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
The bacteriophage receptor sites of Staphylococcus aureus 金黄色葡萄球菌的噬菌体受体部位
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90057-6
Robert R. Rosato , J.A. Cameron
{"title":"The bacteriophage receptor sites of Staphylococcus aureus","authors":"Robert R. Rosato ,&nbsp;J.A. Cameron","doi":"10.1016/0926-6526(64)90057-6","DOIUrl":"10.1016/0926-6526(64)90057-6","url":null,"abstract":"<div><p>This investigation involves an attempt to determine the chemical nature of the bacteriophage receptor site of <em>Staphylococcus aureus</em>. Cell walls of the propagating strain of phage 77 were isolated by sonic oscillation and density-gradient centrifugation. They were further disintegrated by sonic oscillation and fractionated. Fractions were tested for phage-inactivating ability and attachment of phage. Particles of approx. 200 mμ diameter were isolated which were capable of both inactivation and attachment.</p><p>Chemical analysis of these particles indicated no nucleic acid, protein or polysaccharide. The amino acids glycine, lysine, alanine and glutamic acid were found by paper chromatography. Muramic acid and <em>N</em>-<em>acetylglucosamine</em> were also found. These findings indicate that the phage receptor site of the Staphylococcus cell wall is a mucopeptide. Teichoic acid has not been implicated. Inactivating ability was destroyed by heat and trichloroacetic acid. It was enhanced by trypsin (EC 3.4.4.4.) and lysozyme (EC 3.2.1.17).</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 113-119"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90057-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
The conformation of hyaluronic acid and chondroitin sulfate C: The metachromatic reaction 透明质酸与硫酸软骨素C的构象:异色反应
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90049-7
M.D. Schoenberg, R.D. Moore
{"title":"The conformation of hyaluronic acid and chondroitin sulfate C: The metachromatic reaction","authors":"M.D. Schoenberg,&nbsp;R.D. Moore","doi":"10.1016/0926-6526(64)90049-7","DOIUrl":"10.1016/0926-6526(64)90049-7","url":null,"abstract":"<div><p>The absorption characteristics of complexes of hyaluronic acid and chondroitin sulfate C with toluidine blue were studied. From the physical requirements necessary to form the metachromatic complexes, suggestions have been made concerning the conformation of the two polysaccharides.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 42-51"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90049-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
A γ-globulin glycoprotein in bovine vitreous body 牛玻璃体中一种γ-球蛋白糖蛋白
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90047-3
E.R. Berman
{"title":"A γ-globulin glycoprotein in bovine vitreous body","authors":"E.R. Berman","doi":"10.1016/0926-6526(64)90047-3","DOIUrl":"https://doi.org/10.1016/0926-6526(64)90047-3","url":null,"abstract":"<div><p>A glycoprotein containing 4.3% neutral sugar, 2.07% amino sugar and 0.4% sialic acid has been isolated from bovine vitreous body. It is electrophoretically homogeneous and has a mobility of −2.2·10<sup>−5</sup> cm<sup>2</sup>/V sec, which places it among the γ-globulins. The sedimentation coefficient and diffusion constant indicate a molecular weight slightly lower than the generally accepted values for γ-globulins. Other examples of connective tissue proteins which resemble, but are not identical to, plasma proteins are discussed.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 27-34"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90047-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91622671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Determination of glycosaminoglycans (mucopolysaccharides) from tissues on the microgram scale 组织中糖胺聚糖(粘多糖)的微克级测定
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90045-X
C.A. Antonopoulos , S. Gardell , J.A. Szirmai , Ellen R. De Tyssonsk
{"title":"Determination of glycosaminoglycans (mucopolysaccharides) from tissues on the microgram scale","authors":"C.A. Antonopoulos ,&nbsp;S. Gardell ,&nbsp;J.A. Szirmai ,&nbsp;Ellen R. De Tyssonsk","doi":"10.1016/0926-6526(64)90045-X","DOIUrl":"https://doi.org/10.1016/0926-6526(64)90045-X","url":null,"abstract":"<div><p>Microgram quantities of glycosaminoglycans can be quantitatively liberated from tissues, fractionated and determined by the following procedure: (1) Digestion of the tissue sample with papain (EC 3.4.4.10). (2) Precipitation of the glycosaminoglycans as their cetylpyridinium complexes on an inert support of powdered cellulose packed in a column. (3) Fractional elution of the complexes from the column with salt solutions of increasing concentration. (4) Estimation of the amount of glycosaminoglycans in each fraction after hydrolysis followed by determination of the hexosamines by a modified <span>Elson and Morgan</span> method.</p><p>Experimental data are presented to validate the various steps of the procedure. The results were found to be reproducible and accurate for fractions down to 2–5 μg of hexosamine. Small differences in the solubility properties of the glycosaminoglycan-cetylpyridinium complexes could be detected and visualized by the construction of “solubility profiles”.</p><p>Application of the procedure to histological sections of the intervertebral disc and the nasal septum cartilage revealed differences in the amount and properties of glycosaminoglycans in tissue structures 100–200 μ apart. The method should be useful to study the distribution of glycosaminoglycans at the tissue level.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 1-19"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90045-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91635351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 272
Application of mass spectrometry to methylated monosaccharides identification 质谱法在甲基化单糖鉴定中的应用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-03-02 DOI: 10.1016/0926-6526(64)90064-3
N.K. Kochetkov, O.S. Chizhov
{"title":"Application of mass spectrometry to methylated monosaccharides identification","authors":"N.K. Kochetkov,&nbsp;O.S. Chizhov","doi":"10.1016/0926-6526(64)90064-3","DOIUrl":"10.1016/0926-6526(64)90064-3","url":null,"abstract":"","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 1","pages":"Pages 134-136"},"PeriodicalIF":0.0,"publicationDate":"1964-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90064-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23735964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
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