从牛肉角膜分离硫酸角蛋白的变异

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-11-01 DOI:10.1016/0926-6526(64)90006-0

Bernard Wortman

{"title":"从牛肉角膜分离硫酸角蛋白的变异","authors":"Bernard Wortman","doi":"10.1016/0926-6526(64)90006-0","DOIUrl":null,"url":null,"abstract":"<div><p>Glycosaminoglycans were isolated from beef cornea and further fractionated on the basis of ethanol solubility. Variability of keratan sulfate was found. The forms differ in reducing activity, sulfur content, infrared spectra and specific optical rotation. Keratan sulfotransferase (3′-phosphoadenylylsulfate: keratan sulfotransferase) activity is low but detectable in beef cornea-epithelial extract. Keratan sulfate with the lower sulfate content acts as a sulfate acceptor while the other does not.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 288-295"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90006-0","citationCount":"11","resultStr":"{\"title\":\"Variability of keratan sulfate isolated from beef cornea\",\"authors\":\"Bernard Wortman\",\"doi\":\"10.1016/0926-6526(64)90006-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Glycosaminoglycans were isolated from beef cornea and further fractionated on the basis of ethanol solubility. Variability of keratan sulfate was found. The forms differ in reducing activity, sulfur content, infrared spectra and specific optical rotation. Keratan sulfotransferase (3′-phosphoadenylylsulfate: keratan sulfotransferase) activity is low but detectable in beef cornea-epithelial extract. Keratan sulfate with the lower sulfate content acts as a sulfate acceptor while the other does not.</p></div>\",\"PeriodicalId\":100172,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides\",\"volume\":\"83 3\",\"pages\":\"Pages 288-295\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6526(64)90006-0\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926652664900060\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926652664900060","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 11

摘要

从牛肉角膜中分离出糖胺聚糖，并根据乙醇溶解度对其进行进一步分离。发现了硫酸角蛋白的变异性。这些形式在还原活性、硫含量、红外光谱和比旋光度上有所不同。角蛋白硫转移酶(3 ' -磷酸腺苷硫酸酯:角蛋白硫转移酶)活性低，但在牛肉角膜上皮提取物中可检测到。硫酸盐含量较低的硫酸角聚糖作为硫酸盐受体，而另一种则不起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Variability of keratan sulfate isolated from beef cornea

Glycosaminoglycans were isolated from beef cornea and further fractionated on the basis of ethanol solubility. Variability of keratan sulfate was found. The forms differ in reducing activity, sulfur content, infrared spectra and specific optical rotation. Keratan sulfotransferase (3′-phosphoadenylylsulfate: keratan sulfotransferase) activity is low but detectable in beef cornea-epithelial extract. Keratan sulfate with the lower sulfate content acts as a sulfate acceptor while the other does not.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides

自引率

0.00%

发文量