Martin I. Horowitz, Yohichi Hashimoto, Ward Pigman
{"title":"牛颌下粘蛋白的血群物质活性ⅰ。牛颌下粘蛋白的活性和牛颌下粘蛋白糖肽的性质","authors":"Martin I. Horowitz, Yohichi Hashimoto, Ward Pigman","doi":"10.1016/0926-6526(64)90037-0","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The presence of blood-group-substance activity in bovine saliva prompted investigation of the immunologic behavior of bovine submaxillary mucin and of glycopeptides obtained by proteolytic digestion of the mucin.</p></span></li><li><span>2.</span><span><p>2. Purified preparations of bovine submaxillary mucin inhibited hemolysis in the bovine J-anti-J system. Removal of sialic acid from the mucin by mild acid hydrolysis increased the potency of the preparation. Periodate oxidation of sialic acid free-bovine submaxillary mucin destroyed the blood-group J activity.</p></span></li><li><span>3.</span><span><p>3. Bovine submaxillary mucin exhibited a high degree of specificity. it did not inhibit other bovine blood group systems, and it was inactive in human B, M, N and I, i systems; bovine submaxillary mucin had moderate inhibition in the A and O (H) systems.</p></span></li><li><span>4.</span><span><p>4. Bovine submaxillary mucin was digested by pronase and papain (EC 3.4.4.10), and the glycopeptides were fractionated through Sephadex G-50. Glycopeptide fractions which contained the greatest amounts of hexosamine, galactose, and fucose relative to sialic acid were the best glycopeptide inhibitors of J hemolysis.</p></span></li><li><span>5.</span><span><p>5. Bovine submaxillary mucin cross-reacted with antiserum to Type XIV pneumococcal polysaccharide only after removal of sialic acid from the mucin. Possibly some <span>d</span>-galactosyl residues were exposed by the removal of sialic acid from bovine submaxillary mucin.</p></span></li></ul></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 2","pages":"Pages 209-217"},"PeriodicalIF":0.0000,"publicationDate":"1964-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90037-0","citationCount":"3","resultStr":"{\"title\":\"Blood-group-substance activity of bovine submaxillary mucin I. J activity of bovine submaxillary mucin and properties of bovine submaxillary mucin glycopeptides\",\"authors\":\"Martin I. Horowitz, Yohichi Hashimoto, Ward Pigman\",\"doi\":\"10.1016/0926-6526(64)90037-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The presence of blood-group-substance activity in bovine saliva prompted investigation of the immunologic behavior of bovine submaxillary mucin and of glycopeptides obtained by proteolytic digestion of the mucin.</p></span></li><li><span>2.</span><span><p>2. Purified preparations of bovine submaxillary mucin inhibited hemolysis in the bovine J-anti-J system. Removal of sialic acid from the mucin by mild acid hydrolysis increased the potency of the preparation. Periodate oxidation of sialic acid free-bovine submaxillary mucin destroyed the blood-group J activity.</p></span></li><li><span>3.</span><span><p>3. Bovine submaxillary mucin exhibited a high degree of specificity. it did not inhibit other bovine blood group systems, and it was inactive in human B, M, N and I, i systems; bovine submaxillary mucin had moderate inhibition in the A and O (H) systems.</p></span></li><li><span>4.</span><span><p>4. Bovine submaxillary mucin was digested by pronase and papain (EC 3.4.4.10), and the glycopeptides were fractionated through Sephadex G-50. Glycopeptide fractions which contained the greatest amounts of hexosamine, galactose, and fucose relative to sialic acid were the best glycopeptide inhibitors of J hemolysis.</p></span></li><li><span>5.</span><span><p>5. Bovine submaxillary mucin cross-reacted with antiserum to Type XIV pneumococcal polysaccharide only after removal of sialic acid from the mucin. Possibly some <span>d</span>-galactosyl residues were exposed by the removal of sialic acid from bovine submaxillary mucin.</p></span></li></ul></div>\",\"PeriodicalId\":100172,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides\",\"volume\":\"83 2\",\"pages\":\"Pages 209-217\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6526(64)90037-0\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926652664900370\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926652664900370","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Blood-group-substance activity of bovine submaxillary mucin I. J activity of bovine submaxillary mucin and properties of bovine submaxillary mucin glycopeptides
1.
1. The presence of blood-group-substance activity in bovine saliva prompted investigation of the immunologic behavior of bovine submaxillary mucin and of glycopeptides obtained by proteolytic digestion of the mucin.
2.
2. Purified preparations of bovine submaxillary mucin inhibited hemolysis in the bovine J-anti-J system. Removal of sialic acid from the mucin by mild acid hydrolysis increased the potency of the preparation. Periodate oxidation of sialic acid free-bovine submaxillary mucin destroyed the blood-group J activity.
3.
3. Bovine submaxillary mucin exhibited a high degree of specificity. it did not inhibit other bovine blood group systems, and it was inactive in human B, M, N and I, i systems; bovine submaxillary mucin had moderate inhibition in the A and O (H) systems.
4.
4. Bovine submaxillary mucin was digested by pronase and papain (EC 3.4.4.10), and the glycopeptides were fractionated through Sephadex G-50. Glycopeptide fractions which contained the greatest amounts of hexosamine, galactose, and fucose relative to sialic acid were the best glycopeptide inhibitors of J hemolysis.
5.
5. Bovine submaxillary mucin cross-reacted with antiserum to Type XIV pneumococcal polysaccharide only after removal of sialic acid from the mucin. Possibly some d-galactosyl residues were exposed by the removal of sialic acid from bovine submaxillary mucin.
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