工程技术最新文献

{"title":"Attenuated heterogeneity of hippocampal neuron subsets in response to novelty induced by amyloid-β.","authors":"Xiaoxin Ren, Yimeng Wang, Xin Li, Xueling Wang, Zhaodi Liu, Jiajia Yang, Ling Wang, Chenguang Zheng","doi":"10.1007/s11571-025-10237-x","DOIUrl":"10.1007/s11571-025-10237-x","url":null,"abstract":"<p><p>Alzheimer's disease (AD) patients exhibited episodic memory impairments including location-object recognition in a spatial environment, which was also presented in animal models with amyloid-β (Aβ) accumulation. A potential cellular mechanism was the unstable representation of spatial information and lack of discrimination ability of novel stimulus in the hippocampal place cells. However, how the firing characteristics of different hippocampal subsets responding to diverse spatial information were interrupted by Aβ accumulation remains unclear. In this study, we observed impaired novel object-location recognition in Aβ-treated Long-Evans rats, with larger receptive fields of place cells in hippocampal CA1, compared with those in the saline-treated group. We identified two subsets of place cells coding object information (ObjCell) and global environment (EnvCell) during the task, with firing heterogeneity in response to introduced novel information. ObjCells displayed a dynamic representation responding to the introduction of novel information, while EnvCells exhibited a stable representation to support the recognition of the familiar environment. However, the dynamic firing patterns of these two subsets of cells were disrupted to present attenuated heterogeneity under Aβ accumulation. The impaired spatial representation novelty information could be due to the disturbed gamma modulation of neural activities. Taken together, these findings provide new evidence for novelty recognition impairments of AD rats with spatial representation dysfunctions of hippocampal subsets.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"56"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered effective connectivity within brain lesioned regions and cognitive impairment after stroke.","authors":"Jing Zhang, Hui Tang, Lijun Zuo, Hao Liu, Zixiao Li, Jing Jing, Yongjun Wang, Tao Liu","doi":"10.1007/s11571-024-10209-7","DOIUrl":"10.1007/s11571-024-10209-7","url":null,"abstract":"<p><p>Poststroke cognitive impairments (PSCI) reflect widespread network dysfunction due to structural damage, abnormal neural activity, or abnormal connections in affected brain regions. The exact influence of these lesioned regions on the related functional network and their role in PSCI remains unclear. We recruited 35 first-time stroke patients who had basal ganglia infarcts and PSCI, along with 29 age-matched healthy controls. We utilized T1-weighted imaging to inspect structural damage with regional gray matter volume (GMV). Resting-state fMRI data were utilized to examine spontaneous activities with regional Wavelet-ALFF metric, investigate dynamic functional connectivity (dFC) by seeding the region with damaged GMV, and further study effective connectivity within the abnormal dFC network and its impact on PSCI. In comparison to HC, patients showed significant reduced GMV in the bilateral Rolandic operculum (ROL), along with notable abnormal Wavelet-ALFF values in the right Precuneus (PCUN) and left Cerebellum_9 (CER9). Particularly, an abnormal dFC network seeded in the left ROL, demonstrating significantly differential between PSCI and HC groups and remaining consistent across all time windows, was observed. This abnormal dFC network comprised the left ROL as the seed region, the right ROL, bilateral PCUN, bilateral CER9, right Superior Temporal Gyrus (STG), and right Parahippocampal Gyrus (PHG). Notably, in patients, impaired functions across various cognitive domains significantly influenced the altered effective connections among the abnormal regions, particularly impacting the connections between structurally damaged regions and those with abnormal spontaneous activity. These findings suggest that altered effective connectivity networks within lesioned regions may contribute to deficits in various cognitive domains in PSCI.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11571-024-10209-7.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"36"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/21691401.2025.2473244","DOIUrl":"https://doi.org/10.1080/21691401.2025.2473244","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"87"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture.","authors":"Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta","doi":"10.1080/21691401.2025.2475099","DOIUrl":"10.1080/21691401.2025.2475099","url":null,"abstract":"<p><p>Tendon injuries significantly impact quality of life, prompting the exploration of innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Tendon Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in a Growth-Differentiation Factor-5-supplemented medium to promote tenogenic differentiation under static and dynamic conditions using a custom-made perfusion bioreactor. Once at 80% confluence, cells were transitioned to a serum-free medium for conditioned media collection. Ultracentrifugation revealed the presence of vesicles with a 10⁶ particles/mL concentration and sub-200nm diameter size. Dynamic culture yielded a 3-fold increase in EV protein content compared to static culture, as confirmed by Western-blot analysis. Differences in surface marker expression were also shown by flow cytometric analysis. Data suggest that we efficiently developed a protocol for extracting EVs from human TSPCs, particularly under dynamic conditions. This approach enhances EV protein content, offering potential therapeutic benefits for tendon regeneration. However, further research is needed to fully understand the role of EVs in tendon regeneration.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-16"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The integration of metabolites from <i>Forsythia suspensa</i> and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies.","authors":"Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang","doi":"10.1080/21691401.2025.2475088","DOIUrl":"10.1080/21691401.2025.2475088","url":null,"abstract":"<p><p>This study integrates metabolites from Forsythia suspensa (FS) and gut microbiota GM to assess combined therapeutic efficacy against drug-induced liver injury (DILI) using network pharmacology and molecular docking. Metabolites of FS and GM were retrieved from the NPASS and gutMGene databases, respectively. Relevant targets for metabolites and DILI-related targets were identified through public databases. The PPI network and KEGG pathway analysis were employed to identify hub targets and key signalling pathways. Furthermore, we performed a molecular docking assay on the active metabolites and targets to verify the network pharmacological concept. The physicochemical properties and toxicity of identified key metabolites were assessed using in silico platforms. 19 final targets were recognized as key proteins responsible for the alleviation of DILI by FS and GM metabolites, with ESR1 emerging as a central target in the PPI network. The estrogen signalling pathway, particularly involving ESR1, ESR2 and JUN genes, was identified as a key mediator in the therapeutic effects. Four GM metabolites (baicalein, luteolin, lunularin and 2,3-bis(3,4-dihydroxybenzyl)butyrolactone) and two FS metabolites (pinoresinol and isolariciresinol) were identified as non-toxic, promising candidates. In conclusion, metabolites from FS and GM may exert a potent synergistic effect on DILI through modulation of the estrogen signalling pathway.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"105-121"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic modeling of astrocyte-neuron interactions under the influence of Aβ deposition.","authors":"JiangNing Wang, XiaoLi Yang","doi":"10.1007/s11571-025-10246-w","DOIUrl":"https://doi.org/10.1007/s11571-025-10246-w","url":null,"abstract":"<p><p>β-amyloid (Aβ) protein accumulation is recognized as a key factor in Alzheimer's disease (AD) pathogenesis. Its effects on astrocyte function appear primarily as disturbances to intracellular calcium signaling, which, in turn, affects neuronal excitability. We propose an innovative neuron-astrocyte interaction model to examine how Aβ accumulation influences astrocyte calcium oscillation and neuronal excitability, emphasizing its significance in AD pathogenesis. This comprehensive model describes not only the response of the astrocyte to presynaptic neuron stimulation but also the release of the downstream signaling glutamate and its consequential feedback on neurons. Our research concentrates on changes within two prominent pathways affected by Aβ: the creation of Aβ astrocyte membrane pores and the enhanced sensitivity of ryanodine receptors. By incorporating these adjustments into our astrocyte model, we can reproduce previous experimental findings regarding aberrant astrocyte calcium activity and neural behavior associated with Aβ from a neural computational viewpoint. Within a specified range of Aβ influence, our numerical analysis reveals that astrocyte cytoplasmic calcium rises, calcium oscillation frequency increases, and the time to the first calcium peak shortens, indicating the disrupted astrocyte calcium signaling. Simultaneously, the neuronal firing rate and cytosolic calcium concentration increase while the threshold current for initiating repetitive firing diminishes, implying heightened neuronal excitability. Given that increased neuronal excitability commonly occurs in early AD patients and correlates with cognitive decline, our findings may highlight the importance of Aβ accumulation in AD pathogenesis and provide a theoretical basis for identifying neuronal markers in the early stages of the disease.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"60"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanistic action of mogroside V in the alleviation of oxidative aging.","authors":"Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen","doi":"10.1080/21691401.2025.2486752","DOIUrl":"https://doi.org/10.1080/21691401.2025.2486752","url":null,"abstract":"<p><strong>Introduce: </strong>Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.</p><p><strong>Methods: </strong>We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.</p><p><strong>Results: </strong>By down-regulating the <i>EGFR</i>/<i>p38</i>/<i>JNK</i> pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of <i>Ptp1b</i> mRNA had increased significantly while the levels of <i>c-Jun</i> mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including <i>Bacteroidales</i> and <i>Lactobacillaceae</i>.</p><p><strong>Conclusion: </strong>Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"166-180"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilevel Inter-modal and Intra-modal Transformer network with domain adversarial learning for multimodal sleep staging.","authors":"Yang-Yang He, Jian-Wei Liu","doi":"10.1007/s11571-025-10262-w","DOIUrl":"10.1007/s11571-025-10262-w","url":null,"abstract":"<p><p>Sleep staging identification is a fundamental task for the diagnosis of sleep disorders. With the development of biosensing technology and deep learning technology, it is possible to automatically decode sleep process through electroencephalogram signals. However, most sleep staging methods do not consider multimodal sleep signals such as electroencephalogram and electrooculograms signals simultaneously for sleep staging due to the limitation of performance improvement. To this regard, we design a Multilevel Inter-modal and Intra-modal Transformer network with domain adversarial learning for multimodal sleep staging, we introduce a multilevel Transformer structure to fully capture the temporal dependencies within sleep signals of each modality and the interdependencies among different modalities. Simultaneously, we strive for the multi-scale CNNs to learn time and frequency features separately. Our research promotes the application of Transformer models in the field of sleep staging identification. Moreover, considering individual differences among subjects, models trained on one group's data often perform poorly when applied to another group, known as the domain generalization problem. While domain adaptation methods are commonly used, fine-tuning on the target domain each time is cumbersome and impractical. To effectively address these issues without using target domain information, we introduce domain adversarial learning to help the model learn domain-invariant features for better generalization across domains. We validated the superiority of our model on two commonly used datasets, significantly outperforming other baseline models. Our model efficiently extracts dependencies of intra-modal level and inter-modal level from multimodal sleep data, making it suitable for scenarios requiring high accuracy.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"80"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of type II chalcone isomerase (<i>CHI</i>) genes in regulating soybean (<i>Glycine max L</i>.) nodule formation.","authors":"Xinyue Wang, Jingwen Li, Yuxue Zhou, Jinhao Zhang, Le Wang, Yajing Liu, Xuguang Yang, Hongshuang Han, Qingyu Wang, Ying Wang","doi":"10.1080/21645698.2025.2486280","DOIUrl":"10.1080/21645698.2025.2486280","url":null,"abstract":"<p><p>Biological nitrogen fixation (BNF) is the most cost-effective and environmentally benign method for nitrogen fertilization. Isoflavones are important signaling factors for BNF in leguminous plants. Whether chalcone isomerase (<i>CHI</i>), the key enzyme gene in the flavonoid synthesis pathway, contributes to soybean (<i>Glycine max</i>) nodulation has not yet been fully clarified. In the present study, we identified the functions of three types of <i>GmCHI</i> for BNF using a hairy root system. The results showed that <i>GmCHI1A</i> and <i>GmCHI1B1</i> positively increased nodulation while <i>GmCHI1B2</i> did not, with the <i>GmCHI1A</i> gene having a greater effect than <i>GmCHI1B1</i>. Meanwhile, the daidzein and genistein contents were significantly increased in composite plants overexpressing <i>GmCHI1A</i> and reduced in composite plants, thus interfering with <i>GmCHI1A</i>. However, overexpression of <i>GmCHI1B1</i> significantly increased the content of glycitein but not daidzein, genistein content implied that homologous genes exhibit functional differentiation. These results provide a reference for subsequent studies on improving nitrogen fixation in soybeans and providing functional genes for the improvement of new varieties.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"305-317"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat.","authors":"Naglaa A Abdallah, Hany Elsharawy, Hamiss A Abulela, Roger Thilmony, Abdelhadi A Abdelhadi, Nagwa I Elarabi","doi":"10.1080/21645698.2022.2120313","DOIUrl":"10.1080/21645698.2022.2120313","url":null,"abstract":"<p><p>Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3'(2'), 5'-bisphosphate nucleotidase (<i>TaSal1</i>) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active <i>TaSal1</i> homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat <i>TaSal1</i> genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable <i>TaSal1</i> mutations in the M₁ progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and bended stems, but there were no significant changes in the internode length and width, leaf morphology, and stem shape. Anatomical and scanning electron microscope studies of the young leaves of mutated <i>TaSal1</i> lines showed closed stomata, increased stomata width and increase in the size of the bulliform cells. <i>Sal1</i> mutant seedlings germinated and grew better on media containing polyethylene glycol than wildtype seedlings. Our results indicate that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing is efficient tool for mutating more multiple TaSal1 loci in hexaploid wheat.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-17"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33490173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}