Chemistry & Biodiversity最新文献

{"title":"Physicochemical Characterization and In Vitro Hypoglycemic Activity of a Low-Molecular-Weight Extracellular Polysaccharide From Deep-Sea Beauveria bassiana.","authors":"Xu Qiu, Lihang Jiao, Jingjing Xue, Guangxin Xu, Shen Yang, Xixiang Tang","doi":"10.1002/cbdv.202503649","DOIUrl":"https://doi.org/10.1002/cbdv.202503649","url":null,"abstract":"<p><p>Deep-sea microorganisms constitute a valuable source of structurally diverse natural products. In this study, an extracellular polysaccharide was isolated from the deep-sea fungus Beauveria bassiana T2-2 by ultrasonic-assisted hot water extraction followed by Sephadex LH-20 chromatography, yielding a fraction designated BPS-2. Physicochemical characterization revealed that BPS-2 is a glucose-rich polysaccharide (>90% molar ratio) with a weight-average molecular weight of approximately 3.62 kDa and moderate levels of uronic acid (2.84%) and sulfate (3.15%). In vitro enzyme assays demonstrated that BPS-2 inhibited α-amylase and α-glucosidase in a concentration-dependent manner, with an IC₅₀ value of 1.3 × 10^-4 M for α-glucosidase. Within the tested range, its inhibitory activity was stronger than that of Astragalus polysaccharide (APS) but weaker than that of acarbose. In a palmitic acid-induced insulin-resistant HepG2 model, BPS-2 significantly enhanced glucose consumption and increased 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2-NBDG) uptake by 44.9% at 0.75 mg/mL without detectable cytotoxicity, suggesting a potential role in improving cellular glucose utilization under insulin-resistant conditions. To the best of our knowledge, this is the first report describing hypoglycemic-related activity of extracellular polysaccharides derived from deep-sea B. bassiana. These findings provide initial evidence linking the structural features of BPS-2 to glucose metabolism-associated bioactivity, while further studies are required to clarify detailed structural characteristics and underlying molecular mechanisms.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03649"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal Potential of Essential Oils from Origanum onites, Lavandula angustifolia, and Foeniculum vulgare Against Agonoscena pistaciae: Integrated In Vitro and In Silico Approaches.","authors":"Cemile Yaş, Halil Dilmen, Mesut Gok, Yunus Başar","doi":"10.1002/cbdv.202503318","DOIUrl":"https://doi.org/10.1002/cbdv.202503318","url":null,"abstract":"<p><p>This study evaluated the insecticidal potential of essential oils (EOs) obtained from Origanum onites, Lavandula angustifolia, and Foeniculum vulgare against Agonoscena pistaciae. The phytochemical content of the essential oils was determined by GC-MS. Furthermore, molecular docking analyses were performed to theoretically investigate the inhibitory properties of the dominant compounds against the detoxification enzymes glutathione S-transferase (GST) and cytochrome P450. In the GC-MS analysis, the dominant components were identified as follows: O. onites (60.51% carvacrol, 9.73% linalool, and 7.53% cis-cymene); L. angustifolia (25.44% linalyl acetate, 23.47% linalool, and 7.37% cis-cymene); and F. vulgare (49.41% anethol, 14.62% p-anisaldehyde, and 9.35% estragol). As a result of molecular docking, carvacrol showed the strongest binding affinity to both enzymes, surpassing the reference inhibitors metronidazole and coniferil ferulate, achieving docking scores of -5.40 kcal/mol and -5.70 kcal/mol, respectively. Visualization of molecular interactions revealed strong hydrogen bonds and hydrophobic contacts between carvacrol and the enzyme active sites. Biological analyses confirmed that carvacrol-rich O. onites EOs caused significantly higher mortality rates in A. pistaciae larvae compared to other applications and controls. These results demonstrate that carvacrol derived from O. onites is a promising natural insecticide targeting detoxification enzymes in pests and offering a sustainable alternative to synthetic pesticides.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03318"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemistry and Potential Applications of the Samaras of the Invasive Plant Species Ailanthus altissima (Mill.) Swingle.","authors":"Afef Bejaoui, Emira Noumi, Mejdi Snoussi, Karim Hosni","doi":"10.1002/cbdv.202503628","DOIUrl":"https://doi.org/10.1002/cbdv.202503628","url":null,"abstract":"<p><p>In this study, the samaras of the alien invasive plant species Ailanthus altissima were chemically analyzed and evaluated for their in vitro antioxidant activity and in silico biological activities. Elemental analysis revealed the presence of essential macroelements (Ca > K > P > Mg > Na) and trace elements (Fe > Zn > Cu > Mn > Ni). With their high content (> 12% w/w) and particular fatty acid profile (C18:1 + C18:2 > 90%), samara lipids show limited potential for biodiesel development, as several key physicochemical properties of the prospected biodiesel did not fully adhere to international specifications (ASTM D-6751, EN 14214:2008, BG/T 20828:2007). The phenolic profile, particularly rich in phenolic acids, flavonols, and coumarins, was correlated with strong antioxidant activity. In silico analysis revealed that this untapped feedstock could have promising biological activities with pharmacological potential.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03628"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Characterization of Mannich-Derived 1,4-Naphthoquinones From Lawsone: Photophysical Properties, Molecular Docking, BSA/DNA Interactions, and Antioxidant Activity.","authors":"Yavuz Derin, Büşra Albayrak Misir, Özge Güngör, Seyit Ali Güngör, Raşit Fikret Yilmaz, Taoufik Rohand, Ahmet Tutar","doi":"10.1002/cbdv.202503544","DOIUrl":"https://doi.org/10.1002/cbdv.202503544","url":null,"abstract":"<p><p>The present study focuses on the synthesis of eight novel 2-hydroxy-1,4-naphthoquinone (lawsone) derivatives in moderate to high yields (31%-90%) via the Mannich reaction. The structures of the synthesized compounds (1-8) were characterized using a combination of analytical techniques, including HRMS, as well as ¹H and ¹³C NMR spectroscopy. DNA and BSA binding interactions, along with the antioxidant activities of the newly synthesized 1,4-naphthoquinone derivatives, were systematically evaluated to assess the influence of various substituent groups on their biological performance. Among the tested compounds, compound 2, bearing a para-methyl substituent, exhibited the strongest DNA binding affinity with a binding constant of K_b = 4.33 × 10⁵ M^- ¹. Additionally, ethidium bromide (EB) displacement assays were performed, and corresponding K_sv values were calculated to further elucidate the mode of DNA interaction. BSA binding studies also revealed distinct K_sv values for each compound. Evaluation of antioxidant properties demonstrated that all derivatives exhibited concentration-dependent radical scavenging activity within the range of 6.25-200 µg/mL. Notably, compound 8 exhibited superior antioxidant performance, displaying approximately twice the activity of standard antioxidants. Molecular docking studies were performed on compounds exhibiting the highest biological activity in vitro, and their binding properties and interaction profiles to target proteins were investigated.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03544"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analytical and Electrochemical Strategies for Glycoside Analysis and Detection: Running Head: Analytical Techniques for Detection of Glycosides.","authors":"Arshdeep Chopra, Yogindra Kumari, Samarth Kumar, Renuka Sharma, Nipun Sharma, Ajay Pal Singh, Ankush Kumar, Rohit Bhatia","doi":"10.1002/cbdv.202503581","DOIUrl":"https://doi.org/10.1002/cbdv.202503581","url":null,"abstract":"<p><p>Glycosides represent 16.19% of natural products documented in the dictionary of natural products (DNP), forming a diverse group of secondary metabolites in various plant parts. Structurally, glycosides comprise sugar moieties and aglycones, playing crucial roles in biological systems including cellular stability, enzyme recognition, and defense mechanisms. They exhibit broad pharmacological properties, such as antiviral, antidiabetic, and anticancer effects, often mediated by glycosylation. The identification of glycosides is important for evaluating their potential toxicity and the health risks related to their consumption. Analytical techniques, including chromatographic and electrochemical methods, are central to identifying and quantifying glycosides in biological matrices. Several analytical techniques are examined, including spectroscopic, chromatographic, and electrochemical methods to quantify quercetin glycosides in various samples. UV-vis spectrophotometry is simple and cost-effective; however, it does not offer high selectivity. Chromatographic techniques such as HPLC and GC provide both selectivity and sensitivity, whereas electrochemical methods are known for their high resolution and low detection limits. This review aims to examine the methodologies for determining glycosides in various matrices, focusing on pharmaceutical and biological specimens. Moreover, this study also offers an analytical basis for the development and use of methods for analysing glycosides in real samples.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03581"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity and Cytotoxicity Evaluation of 5-Amino-4-cyano-1,3-oxazole Derivatives: Synthesis, In Silico, and In Vitro Studies.","authors":"Diana Hodyna, Maryna Kachaeva, Yurii Shulha, Olena Trokhimenko, Vasyl Kovalishyn, Volodymyr Brovarets, Larysa Metelytsia","doi":"10.1002/cbdv.202503788","DOIUrl":"https://doi.org/10.1002/cbdv.202503788","url":null,"abstract":"<p><p>Bacterial infections contribute to many human diseases and cancer development, and their increasing incidence, together with rising antimicrobial resistance, underscores the urgent need for new potent biologically active compounds. Quantitative structure-activity relationship (QSAR) modeling was conducted to evaluate a series of oxazole derivatives as antibacterials. Eighteen new 5-amino-4-cyano-1,3-oxazoles with promising predictive properties were synthesized and reliably characterized. Fourteen compounds were found to inhibit bacterial growth (MICs varied from 8 to 512 µg/mL). Eight derivatives exhibited the highest activity against colistin-resistant Escherichia coli and Staphylococcus aureus isolates with MICs of 8-64 µg/mL. Moreover, six compounds exhibited activities greater or similar to that of reference oxacillin and cefepime against clinical isolates. Analysis of the studied 5-amino-4-cyano-1,3-oxazoles as target-directed glutathione S-transferase (GST) inhibitors showed that derivatives 7, 11, and 12 inhibited GSTA1-1 with an IC₅₀ range of 3.2-7.5 µM. Molecular docking results indicate that these compounds can occupy the enzyme's active site. The cytotoxicity results in L20B and RD cell lines showed that compounds 11 and 12 demonstrated significant cytostatic effects, with CD₅₀ values ranging from 0.02 to 2 µg/mL. Additionally, these two compounds showed moderate acute toxicity toward D. magna, with LC₅₀ values of 7.27 ± 1.22 and 5.10 ± 1.07 mg/L (D.R. Passino classification).</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03788"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study on Physicochemical Characteristics and Synergistic Anticancer Effects of Polysaccharides from Different Sources of Radix Bupleuri.","authors":"Xinwen Geng, Shiying Zhao, Jianfeng Gan, Yayun Wu, Lijuan Liu, Ya Zhao, Ruizhi Zhao","doi":"10.1002/cbdv.202503695","DOIUrl":"https://doi.org/10.1002/cbdv.202503695","url":null,"abstract":"<p><p>Radix Bupleuri polysaccharides (RBPs) show synergistic antitumor effects with chemotherapy, but source variation causes inconsistent quality and activity. To evaluate the physicochemical properties and synergistic antitumor activity of RBPs from different species and origins, eight batches from five Bupleurum species were analyzed. Results showed varied physicochemical properties across batches. Polysaccharides from Bupleurum Chinese DC. (BCD) and Bupleurum bicaule Helm (BBH) contained the highest levels of GalA, and Rha, while those from Bupleurum marginatum Wall.ex DC. (BMW), Bupleurum falcatum L. (BFL), and Bupleurum marginatum var. stenophyllum (Wolff) Shan et Y.Li (BMS) were rich in Glc. In combination with cisplatin (DDP), RBPs from BCD showed the strongest synergistic inhibition on Huh7 and A549/DDP cell proliferation. Pearson analysis indicated that Rha and GalA positively correlated with antitumor enhancement, whereas Glc showed a negative effect, suggests that monosaccharide composition is associated with synergistic efficacy.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03695"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-Assisted Synthesis of Oxaphospholene-2-Oxide Derivatives: Biological Evaluation, and In Silico Studies.","authors":"Rayene Sayad, Zineb Aouf, Abdeslem Bouzina, Chahrazed Benzaid, Manel Lina Djendi, Rachida Zerrouki, Nour-Eddine Aouf","doi":"10.1002/cbdv.202503597","DOIUrl":"https://doi.org/10.1002/cbdv.202503597","url":null,"abstract":"<p><p>A practical and efficient approach was developed for the synthesis of novel 1,2-oxaphospholene-2-oxide derivatives, a class of oxygen- and phosphorus-containing heterocycles. This one pot method, performed under microwave (MW) irradiation using provides a rapid and eco-compatible route to access these novel structures. The synthesized compounds were structurally confirmed through ¹H, ¹ ³C, ³¹P NMR, IR, and MS analyses. Their biological potential was explored through antimicrobial and antioxidant evaluations. Several derivatives exhibited promising antibacterial and antifungal activities, particularly in inhibiting microbial growth and biofilm formation, while 2,2-diphenyl-1-pycrilhydrazyl (DPPH) assays revealed moderate to strong antioxidant activity. Complementary in silico studies, including molecular docking, provided insights into the binding affinities and possible interaction modes of the compounds with biological targets. Density functional theory (DFT) calculations further elucidated electronic structures and reactive sites, while ADMET (Absorption, Distribution, Metabolism, Toxicity) predictions using SwissADME highlighted favorable pharmacokinetic and drug-likeness properties.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03597"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgo biloba as a Promising Candidate for Traumatic Brain Injury: Insights From Preclinical and Clinical Studies.","authors":"Jilan A Nazeam, Omnia Mahmoud, Amira Saad, Amira Hassan, Omnia Saleh, Amira Alaaeldein, Ethar Ali, Andrew Ezzat, Ehdaa Bahaa, Andrew Mgahd, Mohamed El-Shazly, Sabah H Elgayed","doi":"10.1002/cbdv.202502901","DOIUrl":"https://doi.org/10.1002/cbdv.202502901","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) is a leading cause of mortality and long-term neurological disability, while effective pharmacological therapies for its neurobehavioral consequences remain limited. This systematic review evaluates the therapeutic potential of Ginkgo biloba (GB), particularly the standardized extract EGb 761, in TBI management. A comprehensive literature search was conducted in PubMed, Scopus, and Web of Science up to February 2026. Sixty-five studies met the inclusion criteria, including 42 in vivo experimental studies and 23 clinical trials examining neuroprotective, cognitive, behavioral, or functional outcomes following GB administration. Preclinical evidence consistently demonstrates that Ginkgo biloba (GB) exerts neuroprotective effects through attenuation of oxidative stress, as evidenced by increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, alongside reduced malondialdehyde (MDA) levels. These effects are accompanied by modulation of the Slc7a11-Eif4ebp1 signaling axis, suppression of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, and regulation of key molecular pathways. Specifically, GB inhibits JAK/STAT and TXNIP/NLRP3 signaling while activating PI3K/AKT-associated pathways and autophagy. Clinical studies indicate potential improvements in cognitive and functional outcomes. However, adverse events, particularly bleeding, have been reported, especially when GB is co-administered with anticoagulants or nonsteroidal anti-inflammatory drugs. This underscores the importance of careful patient monitoring. The current evidence supports the promising neuroprotective potential of GB in traumatic brain injury. Nevertheless, clinical data remain insufficient to justify routine therapeutic use. Large-scale, well-designed, randomized controlled trials are necessary to confirm efficacy, optimize dosing regimens, and establish long-term safety profiles.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e02901"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Content, Antioxidant Activity, Phytochemical Diversity, and Toxicological Potential of Tetradenia riparia (Hochst.) Codd Leaves.","authors":"Caroline Dos Santos Giuliani, Carla Andressa Almeida Farias, Patrícia Kolling Marquezan, Karoline Longo de Aguiar, Elize Musachio, Andreara Rodrigues Dos Reis, Nathália Cardoso de Afonso Bonotto, Fernanda Barbisan, Milene Teixeira Barcia, Ana Lúcia Souza Silva Mateus, Aline Sobreira Bezerra, José Laerte Nörnberg","doi":"10.1002/cbdv.202503506","DOIUrl":"https://doi.org/10.1002/cbdv.202503506","url":null,"abstract":"<p><p>Tetradenia riparia (Hochst.) Codd is a medicinal plant widely used in traditional medicine. However, comprehensive studies integrating its chemical composition, antioxidant capacity, and toxicological safety are still limited. This study aimed to characterize the phenolic content, antioxidant activity, phytochemical diversity, and toxicological potential of T. riparia. The plant material was lyophilized to obtain a homogeneous sample, which was directly used for proximate composition and fatty acid profile. Aqueous extracts prepared from the lyophilized material were employed for the determination of total phenolic content, antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay, toxicological evaluations, and phenolic profiling by HPLC-ESI-QqQ-MS/MS. The chemical analyses revealed a diverse phenolic composition, predominantly composed of phenolic acids and flavonoids, which was associated with relevant antioxidant capacity. Toxicological assessments using human fibroblasts (HFF-1) and genotoxicity assays indicated low toxicity at lower concentrations, supporting the safe use of the species under the evaluated conditions. Overall, these findings provide an integrated chemical and biological assessment, contributing novel data on the phytochemical composition and safety profile of T. riparia.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":"23 5","pages":"e03506"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}