Chemistry & Biodiversity最新文献

Larvicidal Activity of Phytochemicals From Handroanthus impetiginosus Seeds Against Culex quinquefasciatus. 刺手花种子植物化学物质对致倦库蚊的杀幼虫活性研究。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501126

Luana Cristina Diniz Santos, Lucas Santos Azevedo, Ezequias Pessoa de Siqueira, Mairon César Coimbra, Stênio Nunes Alves, Ana Hortência Fonsêca Castro, Luciana Alves Rodrigues Dos Santos Lima

{"title":"Larvicidal Activity of Phytochemicals From Handroanthus impetiginosus Seeds Against Culex quinquefasciatus.","authors":"Luana Cristina Diniz Santos, Lucas Santos Azevedo, Ezequias Pessoa de Siqueira, Mairon César Coimbra, Stênio Nunes Alves, Ana Hortência Fonsêca Castro, Luciana Alves Rodrigues Dos Santos Lima","doi":"10.1002/cbdv.202501126","DOIUrl":"https://doi.org/10.1002/cbdv.202501126","url":null,"abstract":"Handroanthus impetiginosus is a species known to produce bioactive substances, including compounds with larvicidal activity. This study evaluated the toxicity of hexane extract (HE), fatty acids (FAs), and FA methyl esters (FAMEs) from H. impetiginosus seeds against Culex quinquefasciatus larvae. FAs were obtained by hydrolysis of triglycerides present in HE for 30 and 60 min, and FAMEs were produced by transesterification of FAs for the same time. HE was analyzed by gas chromatography-mass spectrometry (GC-MS). Oleic acid and ethyl oleate were found in abundance in HE. HE, FA30s, and FA60s produced mortality rates above 90% in C. quinquefasciatus larvae; FA60s produced a 100% mortality rate at the highest concentration at which they were tested (250 µg/mL). LD50 values ranged from 59.70 µg/mL for HE to 1255.41 µg/mL for FAME60s. LD90 ranged from 194.72 µg/mL for FA30s to 295.56 µg/mL for FA60s. The results indicate that HE and FAs have larvicidal potential.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01126"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignin From Dinizia excelsa: Isolation, Characterization, and Evaluation of Antioxidant, Cytotoxic, Antitumor, Antimicrobial, and Immunomodulatory Activities. 木质素：分离、表征及抗氧化、细胞毒、抗肿瘤、抗菌和免疫调节活性的评价。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501385

Lisandra da Silva Lima, Karla Crystina Costa Dos Santos, Arthur Felix Freire da Silva, Diego Santa Clara Marques, André de Lima Aires, Maria do Carmo Alves de Lima, Iranildo José da Cruz Filho

{"title":"Lignin From Dinizia excelsa: Isolation, Characterization, and Evaluation of Antioxidant, Cytotoxic, Antitumor, Antimicrobial, and Immunomodulatory Activities.","authors":"Lisandra da Silva Lima, Karla Crystina Costa Dos Santos, Arthur Felix Freire da Silva, Diego Santa Clara Marques, André de Lima Aires, Maria do Carmo Alves de Lima, Iranildo José da Cruz Filho","doi":"10.1002/cbdv.202501385","DOIUrl":"https://doi.org/10.1002/cbdv.202501385","url":null,"abstract":"Alkaline lignin extracted from Dinizia excelsa wood was characterized by Fourier-transform infrared (FTIR), ultraviolet-visible (UV-Vis), NMR, gel permeation chromatography (GPC), and thermogravimetric analysis (TGA). Spectra revealed a predominance of guaiacyl units, giving a highly condensed structure with notable thermal stability. Biological assays showed moderate antioxidant activity and no cytotoxicity toward normal cells (viability ≥90.00%). In contrast, significant antitumor effects were observed against MCF‑7 and Jurkat cell lines, together with antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The lignin also stimulated splenocyte proliferation and boosted secretion of IL‑2, IL‑4, IL‑10, and IFN‑γ, indicating an immunomodulatory profile. These results position D. excelsa lignin as a promising phenolic metabolite source for biomedical applications and highlight the importance of Amazonian biodiversity in the search for new bioactive compounds.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01385"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbazole Alkaloids as Antimicrobial and Antibiofilm Agents: A Focus on Plant-Derived Natural Alkaloids. 咔唑类生物碱作为抗菌和抗生物膜剂：植物源性天然生物碱的研究进展。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202500242

Khaled Hussien, Sherouk Hussein Sweilam, Riham A El-Shiekh, Ghadir A Sayed, Mohamed S Abd El Hafeez, Haidy A Abbas, Madonna G Attallah, Marwa Ali-Tammam

{"title":"Carbazole Alkaloids as Antimicrobial and Antibiofilm Agents: A Focus on Plant-Derived Natural Alkaloids.","authors":"Khaled Hussien, Sherouk Hussein Sweilam, Riham A El-Shiekh, Ghadir A Sayed, Mohamed S Abd El Hafeez, Haidy A Abbas, Madonna G Attallah, Marwa Ali-Tammam","doi":"10.1002/cbdv.202500242","DOIUrl":"https://doi.org/10.1002/cbdv.202500242","url":null,"abstract":"With the increasing challenge of antimicrobial resistance (AMR) and a decline in the development of new antimicrobial drugs, there is an urgent need to discover and develop novel agents to combat antibiotic resistance and prevent a potential return to a post-antibiotic era. Various factors contribute to microbial resistance, including both inherent and acquired mechanisms. Biofilm formation stands out as a key virulence determinant in many bacterial infections, significantly enhancing bacterial resistance to antibiotics and evading host defenses. Although overcoming pathogens can be difficult, exploring compounds with diverse modes or mechanisms of action, distinct from conventional drugs, holds promise in tackling AMR. Natural products have long been recognized as a valuable source of bioactive compounds, characterized by diverse structures and functional group chirality. There is an immediate need to investigate natural products, particularly those derived from plants, as potential sources of antimicrobial agents, including antibacterial and antifungal agents. Alkaloids, known for their protective nature, can serve as powerful weapons against resistant microorganisms, either alone or in combination with synthetic antimicrobials. Our review sheds light on carbazole alkaloids and their derivatives as potential antibiotic drugs, highlighting their important mechanisms of action and their potential in eradicating biofilm formation in multidrug-resistant pathogens.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e00242"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted Study of Medicago marina Essential Oil: Chemical Composition, Antidiabetic, and Phytotoxic Insights via In Vitro and In Silico Approaches. 紫花苜蓿精油的多方面研究：化学成分，抗糖尿病，通过体外和计算机方法的植物毒性见解。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501574

Marwa Melliti, Assia Hamdi, Mabrouk Horchani, Eliza de Jesus Barros Dos Santos, Hichem Ben Jannet, Mohammad Faisal, Abdulrahman A Alatar, Marcello Iriti, Mozaniel Santana de Oliveirae, Hayet Edziri

{"title":"Multifaceted Study of Medicago marina Essential Oil: Chemical Composition, Antidiabetic, and Phytotoxic Insights via In Vitro and In Silico Approaches.","authors":"Marwa Melliti, Assia Hamdi, Mabrouk Horchani, Eliza de Jesus Barros Dos Santos, Hichem Ben Jannet, Mohammad Faisal, Abdulrahman A Alatar, Marcello Iriti, Mozaniel Santana de Oliveirae, Hayet Edziri","doi":"10.1002/cbdv.202501574","DOIUrl":"https://doi.org/10.1002/cbdv.202501574","url":null,"abstract":"The essential oil of Medicago marina was subjected to analysis at vegetative and reproductive stages of development, with a particular emphasis on its chemical composition, α-amylase inhibition, and allelopathic activities. The results of the gas chromatography and molecular docking studies demonstrated alterations in oil yield and composition throughout the developmental stages. Before blooming, hydrocarbons were the predominant compounds, while monoterpenes were abundant during the reproductive stage, with higher concentrations of eugenol (8.86%) and β-ionone (17.67%) compared to earlier stages. The α-amylase activity exhibited variation across the growth stages, with the highest enzyme inhibition (87.33 ± 0.001%) observed at 1.25 mg/mL during the reproductive stage. The essential oil demonstrated pronounced allelopathic effects, markedly reducing radish stem elongation (94.09 ± 0.62%) and root growth (95.71 ± 0.2%). Furthermore, molecular docking analysis confirmed that five principal oil components exhibited strong inhibitory activity against human pancreatic α-amylase and 4-hydroxyphenylpyruvate dioxygenase (HPPD). These findings underscore the potential of M. marina essential oil as a hypoglycemic and allelopathic agent, influencing seed germination and the growth of radish and lettuce.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01574"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPLC-ESI/MS-Based Polyphenolic Profiling and Bioactivities of Pistacia atlantica and Oudneya africana: Antioxidant, Anti-Inflammatory, Antimicrobial, and Photoprotective Potential. 基于HPLC-ESI/ ms的大西洋黄连木和非洲乌桕的多酚分析和生物活性：抗氧化、抗炎、抗菌和光保护潜力

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501781

Hajer Tlili, Ahmed Neji, Abdelkarim Ben Arfa, Walid Elfalleh, Chaima Benine, Mohamed Neffati, Hanen Najjaa

{"title":"HPLC-ESI/MS-Based Polyphenolic Profiling and Bioactivities of Pistacia atlantica and Oudneya africana: Antioxidant, Anti-Inflammatory, Antimicrobial, and Photoprotective Potential.","authors":"Hajer Tlili, Ahmed Neji, Abdelkarim Ben Arfa, Walid Elfalleh, Chaima Benine, Mohamed Neffati, Hanen Najjaa","doi":"10.1002/cbdv.202501781","DOIUrl":"https://doi.org/10.1002/cbdv.202501781","url":null,"abstract":"Pistacia atlantica and Oudneya africana are medicinal plants from arid Tunisian regions, traditionally used for their therapeutic properties. This study comparatively evaluated their ethanol and aqueous leaf extracts for phytochemical content and biological activities. LC-ESI-MS identified 19 bioactive compounds, including flavonoids and phenolic acids. The ethanolic extracts exhibited significantly higher total phenolic contents (119.80 ± 2.45 mg GAE/g DW for O. africana vs. 110.14 ± 3.50 mg GAE/g DW for P. atlantica). Antioxidant assays revealed that P. atlantica aqueous extract exhibited the strongest DPPH and ABTS scavenging activities (IC50 = 113 and 8.96 µg/mL, respectively), while O. africana ethanolic extract showed the highest ferric-reducing power (EC50 = 51.00 µg/mL). Antimicrobial testing showed the ethanolic extracts had MIC values as low as 0.625 mg/mL against Candida albicans and Escherichia coli, indicating superior efficacy. Photoprotective analysis revealed P. atlantica ethanolic extract possessed a higher SPF (47.4) than O. africana (39.9), suggesting greater potential as a natural sunscreen agent. These comparative results highlight the advantages of ethanolic extraction for maximizing bioactive compound yield and biological effectiveness and emphasize P. atlantica's stronger potential in antioxidant and photoprotective applications compared to O. africana. This study underscores the value of both species as sources of natural bioactive compounds, with differential properties suited to various medicinal and cosmetic uses.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01781"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Physicochemical Properties and Anti-Fatigue Efficacy of a Polysaccharide-Rich Fraction Derived From Millettia speciosa Champ. 密菇多糖提取物的理化性质及抗疲劳作用。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501744

Fu-Yao Luo, Shuai-Ming Zhu, Hao Ma, Jia Fu, Wen Li, Rui Xu, Yu Yang, Zi-Jia Jin, Chun-Xue Gao, You-Zhi Zhang, Chang-Wei Li, Rui Xue

{"title":"The Physicochemical Properties and Anti-Fatigue Efficacy of a Polysaccharide-Rich Fraction Derived From Millettia speciosa Champ.","authors":"Fu-Yao Luo, Shuai-Ming Zhu, Hao Ma, Jia Fu, Wen Li, Rui Xu, Yu Yang, Zi-Jia Jin, Chun-Xue Gao, You-Zhi Zhang, Chang-Wei Li, Rui Xue","doi":"10.1002/cbdv.202501744","DOIUrl":"https://doi.org/10.1002/cbdv.202501744","url":null,"abstract":"The root of Millettia speciosa Champ has been traditionally and widely used for anti-fatigue properties in folk culture. However, the active components and underlying mechanisms of M. speciosa's anti-fatigue efficacy are still unclear. Notably, polysaccharides are considered the primary component for anti-fatigue action in many traditional Chinese medicines. This study aimed to characterize the physicochemical properties of a polysaccharide-rich fraction from M. speciosa (MSCP) and investigate its anti-fatigue efficacy and underlying mechanisms. The results showed that MSCP was primarily composed of glucose, arabinose, and galactose in a ratio of 90.0:5.2:4.8 and contained two sugar-peaks with molecular weights of 19 544 and 13 166 Da. The behavioral tests showed that MSCP could effectively extend the exhaustive swimming time and running time in the exercise-induced fatigue model and combined stress-induced fatigue model in mice. Treatment with MSCP reduced levels of blood urea nitrogen, blood lactic acid, lactate dehydrogenase, creatine kinase, and malondialdehyde, while increasing levels of muscle glycogen, hepatic glycogen, and superoxide dismutase. Furthermore, MSCP modulated the gut microbiota, which potentially contributes to its anti-fatigue efficacy. Our research provides theoretical support for incorporating MSCP as a novel dietary supplement in functional foods designed to enhance anti-fatigue properties.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01744"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro and In Silico Approaches to Exploring the Potential Therapeutic Effects of Curcuma amada Rhizome Essential Oil. 姜黄根茎精油的体外和计算机实验研究。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501395

Ankita Gogoi, Prajisha Jayaprakash, Twahira Begum, Mohan Lal

{"title":"In Vitro and In Silico Approaches to Exploring the Potential Therapeutic Effects of Curcuma amada Rhizome Essential Oil.","authors":"Ankita Gogoi, Prajisha Jayaprakash, Twahira Begum, Mohan Lal","doi":"10.1002/cbdv.202501395","DOIUrl":"https://doi.org/10.1002/cbdv.202501395","url":null,"abstract":"Curcuma amada (mango ginger) is a medicinal herb valued for its traditional uses and pharmacological properties. This study investigates the essential oil (EO) from C. amada rhizome (Curcuma amada rhizome EO [CAREO]), highlighting its anti-microbial, anti-diabetic, and anti-inflammatory activities through in vitro and in silico methods. Gas chromatography-mass spectrometry (GC-MS) analysis showed that CAREO is rich in monoterpenoids (66.69%) and sesquiterpenes (29.04%), with camphor (52.48%) as the major constituent. Molecular docking revealed strong binding affinities (-4.4 to -9.0 kcal/mol) with different protein targets. CAREO demonstrated superior IC50 values in anti-diabetic (21.03 µg/mL) and anti-inflammatory (21.67 µg/mL) assays compared to standard drugs. It also exhibited significant anti-microbial effects, with different inhibition zones of 15, 18, and 19 mm against Staphylococcus aureus, Salmonella typhimurium, and Aspergillus niger at 500 µg/mL, respectively. Meanwhile, genotoxicity assays yielded negative results, indicating no toxicity. The present work highlights that the anti-microbial, anti-diabetic, and anti-inflammatory activities stand out prominently, as demonstrated by both in vitro and in silico studies. This research emphasizes the pharmacological importance of compounds identified in CAREO, highlighting their potential medical applications and social benefits.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01395"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile Chemical Defense Constituents and Antifungal Mechanisms of Medicago sativa in Response to Ascochyta medicaginicola Infection. 紫花苜蓿挥发性化学防御成分及抗真菌机制研究

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501423

Xunfeng Chen, Fan Li, Dahai Mei, Xuhong Wei, Yongsha Guo, Lijun Gu, Zhibiao Nan, Qiuyan Song

{"title":"Volatile Chemical Defense Constituents and Antifungal Mechanisms of Medicago sativa in Response to Ascochyta medicaginicola Infection.","authors":"Xunfeng Chen, Fan Li, Dahai Mei, Xuhong Wei, Yongsha Guo, Lijun Gu, Zhibiao Nan, Qiuyan Song","doi":"10.1002/cbdv.202501423","DOIUrl":"https://doi.org/10.1002/cbdv.202501423","url":null,"abstract":"Medicago sativa, known as the \"King of Forage,\" is crucial in the feed industry but is threatened by spring black stem and leaf spot caused by Ascochyta medicaginicola. Research on volatile defensive substances against this fungus has been limited. Volatile oils from healthy and diseased alfalfa (inoculated with A. medicaginicola) were collected using dynamic headspace adsorption and analyzed by gas chromatography-mass spectrometry. Key differential metabolites in diseased alfalfa were 1,2-diethylbenzene and 1-(4-ethylphenyl)-ethanone. Antifungal tests showed that 1,2-diethylbenzene had a 12.15% inhibition rate against A. medicaginicola, whereas 1-(4-ethylphenyl)-ethanone achieved a much higher rate of 92.49%. At 23.6 µg/mL, 1-(4-ethylphenyl)-ethanone provided both therapeutic and protective effects against the disease. Its antifungal mechanism included inhibiting pigment deposition, disrupting cell membranes, inducing reactive oxygen species (ROS) accumulation, and triggering apoptosis in mycelial cells. Thus, 1-(4-ethylphenyl)-ethanone shows great potential for controlling alfalfa's black stem disease.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01423"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myricetin Alleviates Formaldehyde-Exacerbated Inflammation in Asthmatic Mice Through Inhibition of Ferrous Iron Accumulation in Pulmonary Macrophages. 杨梅素通过抑制肺巨噬细胞铁积累减轻甲醛加重的哮喘小鼠炎症。

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-31 DOI: 10.1002/cbdv.202501341

Huijuan Ma, Peiyao Wang, Junfeng Wang, Huan Xu

{"title":"Myricetin Alleviates Formaldehyde-Exacerbated Inflammation in Asthmatic Mice Through Inhibition of Ferrous Iron Accumulation in Pulmonary Macrophages.","authors":"Huijuan Ma, Peiyao Wang, Junfeng Wang, Huan Xu","doi":"10.1002/cbdv.202501341","DOIUrl":"https://doi.org/10.1002/cbdv.202501341","url":null,"abstract":"Myricetin, a natural flavonoid known for its anti-inflammatory bioactivity, has also been documented as a chelator of iron in cells. The aim of the study was to examine whether myricetin could alleviate formaldehyde (FA)-exacerbated asthmatic inflammation through chelation of Fe(II). In this study, FA exposure at 2.0 mg/m3 exacerbated inflammation in lung tissues and induced iron accumulation and pro-inflammatory responses in pulmonary macrophages (PMs) in ovalbumin-induced asthmatic murine models, which was abated by deferoxamine via Fe(II) removal. Myricetin administration at 100 mg/kg relieved FA-exacerbated inflammation and alleviated Fe(II) overload in PMs. Myricetin treatment in isolated PMs cultured in vitro at 50 µM also attenuated pro-inflammatory responses of PMs through restoration of iron homeostasis. The results in this study not only showed that FA could enhance inflammatory condition in asthma through increased Fe(II) accumulation in PMs but also demonstrated the anti-inflammatory activity of myricetin via Fe(II) chelation in both FA-exacerbated asthmatic mice and in vitro PMs. Therefore, myricetin has the potential to be developed as a therapeutic agent for inflammatory conditions related to FA exposures.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e01341"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Extraction of Alkaloids From Sophora moorcroftiana Seeds via Enzyme and Ultrasound-Assisted Coupling and Their Antibacterial Activity. 酶-超声耦合高效提取苦参种子生物碱及其抑菌活性研究

IF 2.5 3区化学

Chemistry & Biodiversity Pub Date : 2025-07-30 DOI: 10.1002/cbdv.202500635

Sidan Zhao, Lingxiao He, Ning Ma, Chaoqun You, Yu Zhang, Xun Li, Fei Wang

{"title":"Efficient Extraction of Alkaloids From Sophora moorcroftiana Seeds via Enzyme and Ultrasound-Assisted Coupling and Their Antibacterial Activity.","authors":"Sidan Zhao, Lingxiao He, Ning Ma, Chaoqun You, Yu Zhang, Xun Li, Fei Wang","doi":"10.1002/cbdv.202500635","DOIUrl":"https://doi.org/10.1002/cbdv.202500635","url":null,"abstract":"Sophora moorcroftiana, widely distributed in Xizang and rich in alkaloids, warrants further exploration and utilization. This study aims to analyze the efficiency of enzyme-assisted ultrasonic extraction with the standard solvent method comparatively for the qualitative and quantitative analysis of alkaloids in S. moorcroftiana seeds. The research identified optimal extraction conditions, which included an enzyme (cellulose-hemicellulose complex enzyme) concentration of 10% (w/w), an ultrasonic duration of 45 min, an ultrasonic power of 267.01 W, a solid-liquid ratio of 1:44.82 (v/v), and an ethanol concentration of 77.18%. Under these conditions, the extraction efficiency reached 90.65%, and successfully isolated sophocarpine with a purity of 91.41%. The seeds contained 6.85% moisture and 4.00% alkaloids, with oxymatrine accounting for 2.45%, oxysophocarpine 0.35%, matrine 0.73%, and sophocarpine 0.47%. In addition, the employment of enzymatic hydrolysis-assisted ultrasonic coupling extraction methodology resulted in a 32.5% increase in extraction efficiency compared to the conventional solvent extraction approach. Total alkaloids and the compound sophocarpine demonstrate significant inhibitory effects on both Escherichia coli and Staphylococcus aureus. This study demonstrates that ultrasound-assisted enzyme-coupled extraction is a sustainable and efficient technique with potential applications in the extraction of natural products.","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e00635"},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0