Rosemary Essential Oil Microemulsion for Fungal Keratitis Treatment.

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry & Biodiversity Pub Date : 2025-10-15 DOI:10.1002/cbdv.202502124

Saulo Ribeiro, Mariana Coelho Moraes, Denise de Oliveira Scoaris, Jovita Eugênia Gazinelli Cruz Madeira, Silvia Ligório Fialho, Carolina Paula de Souza Moreira

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Abstract

This study investigated the use of Rosmarinus officinalis (rosemary) essential oil in a microemulsion (ME) formulation for the treatment of ocular fungal keratitis, a serious infection that can lead to blindness. The ME was characterized in terms of pH, stability, homogeneity, osmolarity, and other physicochemical properties. The Hen's Egg Test-Chorioallantoic Membrane assay was used to assess ocular irritancy, and antifungal efficacy was evaluated using the minimum inhibitory concentration method. The oil extraction yielded 1.2%, with eucalyptol (37.89%) as the major compound. The formulation showed a suitable pH (6.96), particle size of 11.35 nm, good stability, and was classified as non-irritant with an irritation score = 1.5. It exhibited strong antifungal activity against Candida parapsilosis (96.1%), C. krusei (100%), C. albicans (99.1%), Fusarium graminearum (90.5%), and Aspergillus parasiticus (79.5%). The results highlight the potential of rosemary essential oil as a base for developing eye drops for the treatment of fungal keratitis.

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迷迭香精油微乳治疗真菌性角膜炎。

本研究调查了迷迭香精油在微乳液（ME）制剂中用于治疗眼部真菌性角膜炎（一种可导致失明的严重感染）的使用。从pH、稳定性、均匀性、渗透压和其他理化性质等方面对ME进行了表征。采用鸡蛋试验-绒毛膜-尿囊膜法评估眼刺激性，采用最小抑制浓度法评估抗真菌效果。桉叶精油提取率为1.2%，主要化合物为桉叶精油（37.89%）。该配方pH值为6.96，粒径为11.35 nm，稳定性好，刺激性评分为1.5，属于无刺激性。对假丝酵母菌（96.1%）、克氏假丝酵母菌（100%）、白色假丝酵母菌（99.1%）、谷物镰刀菌（90.5%）和寄生曲霉（79.5%）具有较强的抑菌活性。研究结果强调了迷迭香精油作为治疗真菌性角膜炎滴眼液基础的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry & Biodiversity 环境科学-化学综合

CiteScore

3.40

自引率

10.30%

发文量

475

审稿时长

2.6 months

期刊介绍： Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.