Cell Regeneration最新文献_第2页

Dasatinib demonstrates efficacy in organoid derived paclitaxel-resistant Trp53/Cdh1-deficient mouse gastric adenocarcinoma with peritoneal metastasis. 达沙替尼对类器官来源的紫杉醇耐药Trp53/ cdh1缺陷小鼠胃腺癌伴腹膜转移有效。

IF 4

Cell Regeneration Pub Date : 2025-04-29 DOI: 10.1186/s13619-025-00232-2

Wenshuai Liu, Lingmeng Li, Leilei Guo, Haojie Li, Zhaoqing Tang, Xuefei Wang, Liyu Huang, Yihong Sun

{"title":"Dasatinib demonstrates efficacy in organoid derived paclitaxel-resistant Trp53/Cdh1-deficient mouse gastric adenocarcinoma with peritoneal metastasis.","authors":"Wenshuai Liu, Lingmeng Li, Leilei Guo, Haojie Li, Zhaoqing Tang, Xuefei Wang, Liyu Huang, Yihong Sun","doi":"10.1186/s13619-025-00232-2","DOIUrl":"https://doi.org/10.1186/s13619-025-00232-2","url":null,"abstract":"Gastric cancer peritoneal metastasis (GCPM) typically indicates a poor clinical prognosis and is frequently observed in diffuse gastric cancer (GC) patients with CDH1 loss of function. GCPM characterized for its aggressiveness and resistance to chemotherapy, most notably paclitaxel (PTX), poses significant treatment challenges. Previously, no mouse gastric adenocarcinoma (MGA) cell lines with Trp53 (encoding mouse p53) and Cdh1 (encoding mouse E-cadherin) mutations and a high potential for peritoneal metastasis in mice have been established. Here, we derived a mouse GC cell line, called MTC, from subcutaneously transplanted mouse Trp53-/-Cdh1-/- GC organoids. Through matching the short tandem repeat profile of MTC with those in current cell banks, we verified the uniqueness of MTC. Furtherly, we confirmed the features of MTC by detecting the expression of p53, E-cadherin, and pan-CK. After long-term exposure of the original MTC line to PTX, we developed a more aggressive, PTX-resistant cell line, termed MTC-R. Compared with MTC, MTC-R demonstrated enhanced tumorigenicity and high potential for peritoneal metastasis in subcutaneous and intraperitoneal tumour models both in BALB/c nude mice and C57BL/6 J mice. Transcriptome analysis revealed the ECM‒receptor interaction pathway activation during the development of PTX resistance, and dasatinib (DASA) was identified as a potential drug targeting this pathway. DASA showed promise in ameliorating disease progression and improving overall survival in MTC-R GCPM model in C57BL/6 J mice. Overall, we established a novel MGA cell line with Trp53 and Cdh1 mutations and its PTX-resistant variant and demonstrated the efficacy of DASA in treating PTX-resistant GCPM.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"16"},"PeriodicalIF":4.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving the spatial organization of fetal liver hematopoiesis by SeekSpace. SeekSpace解决胎儿肝脏造血的空间组织。

IF 4

Cell Regeneration Pub Date : 2025-04-22 DOI: 10.1186/s13619-025-00234-0

Xinyu Thomas Tang, Lin Veronica Chen, Bo O Zhou

{"title":"Resolving the spatial organization of fetal liver hematopoiesis by SeekSpace.","authors":"Xinyu Thomas Tang, Lin Veronica Chen, Bo O Zhou","doi":"10.1186/s13619-025-00234-0","DOIUrl":"https://doi.org/10.1186/s13619-025-00234-0","url":null,"abstract":"The fetal liver is the primary site for the expansion of hematopoietic stem and progenitor cells (HSPCs) during fetal hematopoiesis. However, the spatial organization of different hematopoietic progenitor populations within the fetal liver remains poorly characterized. In this study, we utilized SeekSpace, a high-resolution single-nucleus spatial transcriptomics platform, to map the spatial distribution of hematopoietic stem cells and multipotent progenitor cells (HSC/MPPs) and downstream restricted progenitors (RPs) in relation to other hematopoietic and stromal cell populations in the fetal liver at embryonic day 13.5. Using SeekSpace, we constructed a detailed single-cell spatial transcriptomic atlas of fetal liver hematopoiesis, revealing that both HSC/MPPs and many RPs undergo active expansion in the fetal liver, a process distinct from their behavior in adult bone marrow. Proximity analysis and in situ imaging demonstrated that HSC/MPPs expansion occurs in close association with macrophages and endothelial cells throughout the fetal liver, supported by signaling pathways involving IGF and collagen. In contrast, RPs exhibited no specific spatial proximity to other cell populations during their expansion. Collectively, this study provides a comprehensive resource for understanding the spatial and molecular mechanisms underlying HSC/MPPs and RP expansion during fetal liver hematopoiesis.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"15"},"PeriodicalIF":4.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy in adult stem cell homeostasis, aging, and disease therapy. 自噬在成体干细胞稳态、衰老和疾病治疗中的作用。

IF 4

Cell Regeneration Pub Date : 2025-04-10 DOI: 10.1186/s13619-025-00224-2

Ke Zhao, Indigo T C Chan, Erin H Y Tse, Zhiyao Xie, Tom H Cheung, Yi Arial Zeng

{"title":"Autophagy in adult stem cell homeostasis, aging, and disease therapy.","authors":"Ke Zhao, Indigo T C Chan, Erin H Y Tse, Zhiyao Xie, Tom H Cheung, Yi Arial Zeng","doi":"10.1186/s13619-025-00224-2","DOIUrl":"https://doi.org/10.1186/s13619-025-00224-2","url":null,"abstract":"Autophagy is a crucial cellular process that facilitates the degradation of damaged organelles and protein aggregates, and the recycling of cellular components for the energy production and macromolecule synthesis. It plays an indispensable role in maintaining cellular homeostasis. Over recent decades, research has increasingly focused on the role of autophagy in regulating adult stem cells (SCs). Studies suggest that autophagy modulates various cellular processes and states of adult SCs, including quiescence, proliferation, self-renewal, and differentiation. The primary role of autophagy in these contexts is to sustain homeostasis, withstand stressors, and supply energy. Notably, the dysfunction of adult SCs during aging is correlated with a decline in autophagic activity, suggesting that autophagy is also involved in SC- and aging-associated disorders. Given the diverse cellular processes mediated by autophagy and the intricate mechanisms governing adult SCs, further research is essential to elucidate both universal and cell type-specific regulatory pathways of autophagy. This review discusses the role of autophagy in regulating adult SCs during quiescence, proliferation, self-renewal, and differentiation. Additionally, it summarizes the relationship between SC aging and autophagy, providing therapeutical insights into treating and ameliorating aging-associated diseases and cancers, and ultimately promoting longevity.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"14"},"PeriodicalIF":4.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preserving blood-retinal barrier integrity: a path to retinal ganglion cell protection in glaucoma and traumatic optic neuropathy. 保护血液-视网膜屏障的完整性：青光眼和外伤性视神经病变视网膜神经节细胞保护的途径。

IF 4

Cell Regeneration Pub Date : 2025-04-02 DOI: 10.1186/s13619-025-00228-y

Lai-Yang Zhou, Zhen-Gang Liu, Yong-Quan Sun, Yan-Zhong Li, Zhao-Qian Teng, Chang-Mei Liu

{"title":"Preserving blood-retinal barrier integrity: a path to retinal ganglion cell protection in glaucoma and traumatic optic neuropathy.","authors":"Lai-Yang Zhou, Zhen-Gang Liu, Yong-Quan Sun, Yan-Zhong Li, Zhao-Qian Teng, Chang-Mei Liu","doi":"10.1186/s13619-025-00228-y","DOIUrl":"10.1186/s13619-025-00228-y","url":null,"abstract":"Retinal ganglion cells (RGCs) are the visual gateway of the brain, with their axons converging to form the optic nerve, making them the most vulnerable target in diseases such as glaucoma and traumatic optic neuropathy (TON). In both diseases, the disruption of the blood-retinal barrier(BRB) is considered an important mechanism that accelerates RGC degeneration and hinders axon regeneration. The BRB consists of the inner blood-retinal barrier (iBRB) and the outer blood-retinal barrier (oBRB), which are maintained by endothelial cells(ECs), pericytes(PCs), and retinal pigment epithelial (RPE), respectively. Their functions include regulating nutrient exchange, oxidative stress, and the immune microenvironment. However, in glaucoma and TON, the structural and functional integrity of the BRB is severely damaged due to mechanical stress, inflammatory reactions, and metabolic disorders. Emerging evidence highlights that BRB disruption leads to heightened vascular permeability, immune cell infiltration, and sustained chronic inflammation, creating a hostile microenvironment for RGC survival. Furthermore, the dynamic interplay and imbalance among ECs, PCs, and glial cells within the neurovascular unit (NVU) are pivotal drivers of BRB destruction, exacerbating RGC apoptosis and limiting optic nerve regeneration. The intricate molecular and cellular mechanisms underlying these processes underscore the BRB's critical role in glaucoma and TON pathophysiology while offering a compelling foundation for therapeutic strategies targeting BRB repair and stabilization. This review provides crucial insights and lays a robust groundwork for advancing research on neural regeneration and innovative optic nerve protective strategies.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"13"},"PeriodicalIF":4.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell reprogramming: methods, mechanisms and applications. 细胞重编程：方法、机制和应用。

IF 4

Cell Regeneration Pub Date : 2025-03-27 DOI: 10.1186/s13619-025-00229-x

Fei Zhu, Guangjun Nie

{"title":"Cell reprogramming: methods, mechanisms and applications.","authors":"Fei Zhu, Guangjun Nie","doi":"10.1186/s13619-025-00229-x","DOIUrl":"10.1186/s13619-025-00229-x","url":null,"abstract":"Cell reprogramming represents a powerful approach to achieve the conversion cells of one type into cells of another type of interest, which has substantially changed the landscape in the field of developmental biology, regenerative medicine, disease modeling, drug discovery and cancer immunotherapy. Cell reprogramming is a complex and ordered process that involves the coordination of transcriptional, epigenetic, translational and metabolic changes. Over the past two decades, a range of questions regarding the facilitators/barriers, the trajectories, and the mechanisms of cell reprogramming have been extensively investigated. This review summarizes the recent advances in cell reprogramming mediated by transcription factors or chemical molecules, followed by elaborating on the important roles of biophysical cues in cell reprogramming. Additionally, this review will detail our current understanding of the mechanisms that govern cell reprogramming, including the involvement of the recently discovered biomolecular condensates. Finally, the review discusses the broad applications and future directions of cell reprogramming in developmental biology, disease modeling, drug development, regenerative/rejuvenation therapy, and cancer immunotherapy.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"12"},"PeriodicalIF":4.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNA as an emerging regulator of endoderm differentiation: progress and perspectives. 长链非编码RNA作为一种新兴的内胚层分化调节剂：进展和前景。

IF 4

Cell Regeneration Pub Date : 2025-03-26 DOI: 10.1186/s13619-025-00230-4

Jie Yang, Donghui Zhang, Wei Jiang

引用次数: 0

Standard: human liver-on-a-chip. 标准：人类肝脏芯片。

IF 4

Cell Regeneration Pub Date : 2025-03-24 DOI: 10.1186/s13619-025-00226-0

Haitao Liu, Xu Zhang, Yaqing Wang, Min Zhang, Peng Wang, Jing Shang, Zhongqiang Li, Likun Gong, Xin Xie, Dongyang Liu, Jingbo Pi, Xinghua Gao, Xianliang Li, Wei Ding, Dianbing Wang, Yun Long, Lan Wang, Song Li, Xingchao Geng, Pingkun Zhou, Wanjin Tang, Xian'en Zhang, Chunying Chen, Shengli Yang, Jianhua Qin

{"title":"Standard: human liver-on-a-chip.","authors":"Haitao Liu, Xu Zhang, Yaqing Wang, Min Zhang, Peng Wang, Jing Shang, Zhongqiang Li, Likun Gong, Xin Xie, Dongyang Liu, Jingbo Pi, Xinghua Gao, Xianliang Li, Wei Ding, Dianbing Wang, Yun Long, Lan Wang, Song Li, Xingchao Geng, Pingkun Zhou, Wanjin Tang, Xian'en Zhang, Chunying Chen, Shengli Yang, Jianhua Qin","doi":"10.1186/s13619-025-00226-0","DOIUrl":"10.1186/s13619-025-00226-0","url":null,"abstract":"Organs-on-chips are microphysiological systems designed to replicate key functions of human organs, thereby accelerating innovation in life sciences, such as disease modeling, drug development, and precision medicine. However, the lack of standardized definitions, structural designs, cell sources, model constructions, and functional validations has posed challenges to their widespread translational applications. On April 29, 2024, the Chinese Society of Biotechnology introduced \"Organs-on-chips: Liver\", China's first group standard for human liver-on-a-chip technology. This pioneering standard provides comprehensive guidelines, including scope, terminology, definitions, technical requirements, detection methods, and quality control measures for developing liver models on chips. The introduction of this standard is set to facilitate the establishment of institutional protocols, promote widespread adoption, and drive the international standardization of liver-on-a-chip technologies.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"9"},"PeriodicalIF":4.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissecting endothelial cell heterogeneity with new tools. 用新工具解剖内皮细胞异质性。

IF 4

Cell Regeneration Pub Date : 2025-03-23 DOI: 10.1186/s13619-025-00223-3

Jing Zhong, Rong-Rong Gao, Xin Zhang, Jia-Xin Yang, Yang Liu, Jinjin Ma, Qi Chen

{"title":"Dissecting endothelial cell heterogeneity with new tools.","authors":"Jing Zhong, Rong-Rong Gao, Xin Zhang, Jia-Xin Yang, Yang Liu, Jinjin Ma, Qi Chen","doi":"10.1186/s13619-025-00223-3","DOIUrl":"10.1186/s13619-025-00223-3","url":null,"abstract":"The formation of a blood vessel network is crucial for organ development and regeneration. Over the past three decades, the central molecular mechanisms governing blood vessel growth have been extensively studied. Recent evidence indicates that vascular endothelial cells-the specialized cells lining the inner surface of blood vessels-exhibit significant heterogeneity to meet the specific needs of different organs. This review focuses on the current understanding of endothelial cell heterogeneity, which includes both intra-organ and inter-organ heterogeneity. Intra-organ heterogeneity encompasses arterio-venous and tip-stalk endothelial cell specialization, while inter-organ heterogeneity refers to organ-specific transcriptomic profiles and functions. Advances in single-cell RNA sequencing (scRNA-seq) have enabled the identification of new endothelial subpopulations and the comparison of gene expression patterns across different subsets of endothelial cells. Integrating scRNA-seq with other high-throughput sequencing technologies promises to deepen our understanding of endothelial cell heterogeneity at the epigenetic level and in a spatially resolved context. To further explore human endothelial cell heterogeneity, vascular organoids offer powerful tools for studying gene function in three-dimensional culture systems and for investigating endothelial-tissue interactions using human cells. Developing organ-specific vascular organoids presents unique opportunities to unravel inter-organ endothelial cell heterogeneity and its implications for human disease. Emerging technologies, such as scRNA-seq and vascular organoids, are poised to transform our understanding of endothelial cell heterogeneity and pave the way for innovative therapeutic strategies to address human vascular diseases.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"10"},"PeriodicalIF":4.0,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing bone healing: the role of 3D models. 革命性的骨愈合：3D模型的作用。

IF 4

Cell Regeneration Pub Date : 2025-03-21 DOI: 10.1186/s13619-025-00225-1

Raffaella De Pace, Maria Rosa Iaquinta, Assia Benkhalqui, Antonio D'Agostino, Lorenzo Trevisiol, Riccardo Nocini, Chiara Mazziotta, John Charles Rotondo, Ilaria Bononi, Mauro Tognon, Fernanda Martini, Elisa Mazzoni

{"title":"Revolutionizing bone healing: the role of 3D models.","authors":"Raffaella De Pace, Maria Rosa Iaquinta, Assia Benkhalqui, Antonio D'Agostino, Lorenzo Trevisiol, Riccardo Nocini, Chiara Mazziotta, John Charles Rotondo, Ilaria Bononi, Mauro Tognon, Fernanda Martini, Elisa Mazzoni","doi":"10.1186/s13619-025-00225-1","DOIUrl":"10.1186/s13619-025-00225-1","url":null,"abstract":"The increasing incidence of bone diseases has driven research towards Bone Tissue Engineering (BTE), an innovative discipline that uses biomaterials to develop three-dimensional (3D) scaffolds capable of mimicking the natural environment of bone tissue. Traditional approaches relying on two-dimensional (2D) models have exhibited significant limitations in simulating cellular interactions and the complexity of the bone microenvironment. In response to these challenges, 3D models such as organoids and cellular spheroids have emerged as effective tools for studying bone regeneration. Adult mesenchymal stem cells have proven crucial in this context, as they can differentiate into osteoblasts and contribute to bone tissue repair. Furthermore, the integration of composite biomaterials has shown substantial potential in enhancing bone healing. Advanced technologies like microfluidics offer additional opportunities to create controlled environments for cell culture, facilitating more detailed studies on bone regeneration. These advancements represent a fundamental step forward in the treatment of bone pathologies and the promotion of skeletal health. In this review, we report on the evolution of in vitro culture models applied to the study of bone healing/regrowth, starting from 2 to 3D cultures and microfluids. The different methodologies of in vitro model generation, cells and biomaterials are presented and discussed.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"7"},"PeriodicalIF":4.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies. 人类诱导多能干细胞衍生的中性粒细胞具有很强的抗微生物能力。

IF 4

Cell Regeneration Pub Date : 2025-03-21 DOI: 10.1186/s13619-025-00227-z

Xing Hu, Baoqiang Kang, Mingquan Wang, Huaisong Lin, Zhiyong Liu, Zhishuai Zhang, Jiaming Gu, Yuchan Mai, Xinrui Guo, Wanli Ma, Han Yan, Shuoting Wang, Jingxi Huang, Junwei Wang, Jian Zhang, Tianyu Zhang, Bo Feng, Yanling Zhu, Guangjin Pan

{"title":"Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies.","authors":"Xing Hu, Baoqiang Kang, Mingquan Wang, Huaisong Lin, Zhiyong Liu, Zhishuai Zhang, Jiaming Gu, Yuchan Mai, Xinrui Guo, Wanli Ma, Han Yan, Shuoting Wang, Jingxi Huang, Junwei Wang, Jian Zhang, Tianyu Zhang, Bo Feng, Yanling Zhu, Guangjin Pan","doi":"10.1186/s13619-025-00227-z","DOIUrl":"10.1186/s13619-025-00227-z","url":null,"abstract":"Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented. Here, we develop a scalable approach to generate iNEUs in a chemical defined condition. iNEUs display typical neutrophil characters in terms of phagocytosis, migration, formation of neutrophil extracellular traps (NETs), etc. Importantly, iNEUs display a strong killing potency against various bacteria such as K.pneumoniae, P.aeruginosa, E.coli and S.aureus. Moreover, transfusions of iNEUs in mice with neutrophil dysfunction largely enhance their survival in lethal infection of different bacteria. Together, our data show that hiPSCs derived neutrophils hold strong anti-microbial potencies to protect severe infections under neutrophil dysfunction conditions.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"14 1","pages":"8"},"PeriodicalIF":4.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0