Cell Regeneration最新文献_第10页

TAD reorganization: a new mechanism for cell fate determination. TAD重组:细胞命运决定的新机制。

Cell Regeneration Pub Date : 2021-12-02 DOI: 10.1186/s13619-021-00100-9

Zhou Songyang

引用次数: 1

Diverse biological and engineering strategies towards organ regeneration. 器官再生的多种生物和工程策略。

Cell Regeneration Pub Date : 2021-11-02 DOI: 10.1186/s13619-021-00098-0

Junjie Hou, Qinchao Zhou, Xiaojun Zhu, Jinrong Peng, Jing-Wei Xiong

引用次数: 1

Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium. 单细胞分析确定了子宫颈上皮干细胞的谱系可塑性。

Cell Regeneration Pub Date : 2021-11-01 DOI: 10.1186/s13619-021-00096-2

Zixian Zhao, Yujia Wang, Yingchuan Wu, Dandan Li, Ting Zhang, Yu Ma, Xiaoming Teng, Wei Zuo

{"title":"Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium.","authors":"Zixian Zhao, Yujia Wang, Yingchuan Wu, Dandan Li, Ting Zhang, Yu Ma, Xiaoming Teng, Wei Zuo","doi":"10.1186/s13619-021-00096-2","DOIUrl":"https://doi.org/10.1186/s13619-021-00096-2","url":null,"abstract":"Information about the dynamic change and post-injury regeneration of cervical epithelium is relatively rare, even though it is tightly related to gynecologic malignancy. Here, using a feeder cell-based culturing system, we stably cloned mouse and human P63 and KRT5 expressing cells from the adult cervix as putative cervical stem/progenitor cells (CVSCs). When subjected to differentiation, the cultured cells gave rise to mature cervical epithelium by differentiating into squamous or glandular cells. The ability of endogenous mouse CVSCs to reconstitute cervical epithelium after injury was also evident from the genetic lineage tracing experiments. Single-cell transcriptomic analysis further classified the CVSCs into three subtypes and delineated their bi-lineage differentiation roadmap by pseudo-time analysis. We also tracked the real-time differentiation routes of two representing single CVSC lines in vitro and found that they recapitulated the predicted roadmap in pseudo-time analysis. Signaling pathways including Wnt, TGF-beta, Notch and EGFR were found to regulate the cervical epithelial hierarchy and implicated the different roles of distinct types of cells in tissue homeostasis and tumorigenesis. Collectively, the above data provide a cloning system to achieve stable in vitro culture of a bi-lineage stem/progenitor cell population in the cervix, which has profound implications for our understanding of the cervix stem/progenitor cell function in homeostasis, regeneration, and disease and could be helpful for developing stem cell-based therapies in future.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"10 1","pages":"36"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39578047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Correction to: CRISPR/Cas: a potential gene-editing tool in the nervous system. CRISPR/Cas:神经系统中潜在的基因编辑工具。

Cell Regeneration Pub Date : 2021-10-13 DOI: 10.1186/s13619-021-00095-3

Yanxia Gao, Kexin Gao, Hui Yang

引用次数: 1

Establishment of porcine and monkey colonic organoids for drug toxicity study. 猪和猴结肠类器官药物毒性研究的建立。

Cell Regeneration Pub Date : 2021-10-02 DOI: 10.1186/s13619-021-00094-4

Haonan Li, Yalong Wang, Mengxian Zhang, Hong Wang, Along Cui, Jianguo Zhao, Weizhi Ji, Ye-Guang Chen

引用次数: 5

Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation. 骨骼肌谱系发育和多能干细胞分化的主要调控因子。

Cell Regeneration Pub Date : 2021-10-01 DOI: 10.1186/s13619-021-00093-5

Joana Esteves de Lima, Frédéric Relaix

{"title":"Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation.","authors":"Joana Esteves de Lima, Frédéric Relaix","doi":"10.1186/s13619-021-00093-5","DOIUrl":"https://doi.org/10.1186/s13619-021-00093-5","url":null,"abstract":"In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells, during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinct genetic hierarchies. The major regulators of trunk and limb muscle specification are the paired-homeobox transcription factors PAX3 and PAX7. Distinct gene regulatory networks drive the formation of the different muscles of the head. Despite the redeployment of diverse upstream regulators of muscle progenitor differentiation, the commitment towards the myogenic fate requires the expression of the early myogenic regulatory factors MYF5, MRF4, MYOD and the late differentiation marker MYOG. The expression of these genes is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth. In order to achieve myogenic cell commitment while maintaining an undifferentiated pool of muscle progenitors, several signaling pathways regulate the switch between proliferation and differentiation of myoblasts. The identification of the gene regulatory networks operating during myogenesis is crucial for the development of in vitro protocols to differentiate pluripotent stem cells into myoblasts required for regenerative medicine.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"10 1","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39476793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Coordination of EZH2 and SOX2 specifies human neural fate decision. EZH2和SOX2的协调决定了人类神经的命运。

Cell Regeneration Pub Date : 2021-09-06 DOI: 10.1186/s13619-021-00092-6

Yuan Zhao, Tianyu Wang, Yanqi Zhang, Liang Shi, Cong Zhang, Jingyuan Zhang, Jiao Yao, Qianyu Chen, Xiaofen Zhong, Yanxing Wei, Yongli Shan, Guangjin Pan

{"title":"Coordination of EZH2 and SOX2 specifies human neural fate decision.","authors":"Yuan Zhao, Tianyu Wang, Yanqi Zhang, Liang Shi, Cong Zhang, Jingyuan Zhang, Jiao Yao, Qianyu Chen, Xiaofen Zhong, Yanxing Wei, Yongli Shan, Guangjin Pan","doi":"10.1186/s13619-021-00092-6","DOIUrl":"https://doi.org/10.1186/s13619-021-00092-6","url":null,"abstract":"Polycomb repressive complexes (PRCs) are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells (hESCs), but the underlying molecular basis remains unclear. Here in this study, by employing an array of different approaches, such as gene knock-out, RNA-seq, ChIP-seq, et al., we uncover that EZH2, an important PRC factor, specifies the normal neural fate decision through repressing the competing meso/endoderm program. EZH2-/- hESCs show an aberrant re-activation of meso/endoderm genes during neural induction. At the molecular level, EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural fate. Moreover, EZH2 also supports the proliferation of human neural progenitor cells (NPCs) through repressing the aberrant expression of meso/endoderm program during culture. Together, our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"10 1","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2021-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39405983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Deciphering and reconstitution of positional information in the human brain development. 人脑发育过程中位置信息的破译与重构。

Cell Regeneration Pub Date : 2021-09-01 DOI: 10.1186/s13619-021-00091-7

Yi-Fan Wang, Cong Liu, Peng-Fei Xu

引用次数: 3

The truth lies somewhere in the middle: the cells responsible for liver tissue maintenance finally identified. 真相介于两者之间:负责肝组织维护的细胞终于被发现了。

Cell Regeneration Pub Date : 2021-08-04 DOI: 10.1186/s13619-021-00090-8

Tohru Itoh

引用次数: 0

Role of PTEN-less in cardiac injury, hypertrophy and regeneration. PTEN-less在心脏损伤、肥厚和再生中的作用。

Cell Regeneration Pub Date : 2021-08-02 DOI: 10.1186/s13619-021-00087-3

Tian Liang, Feng Gao, Jinghai Chen

{"title":"Role of PTEN-less in cardiac injury, hypertrophy and regeneration.","authors":"Tian Liang, Feng Gao, Jinghai Chen","doi":"10.1186/s13619-021-00087-3","DOIUrl":"https://doi.org/10.1186/s13619-021-00087-3","url":null,"abstract":"Cardiovascular diseases are the leading cause of death worldwide. Cardiomyocytes are capable of coordinated contractions, which are mainly responsible for pumping blood. When cardiac stress occurs, cardiomyocytes undergo transition from physiological homeostasis to hypertrophic growth, proliferation, or apoptosis. During these processes, many cellular factors and signaling pathways participate. PTEN is a ubiquitous dual-specificity phosphatase and functions by dephosphorylating target proteins or lipids, such as PIP3, a second messenger in the PI3K/AKT signaling pathway. Downregulation of PTEN expression or inhibiting its biologic activity improves heart function, promotes cardiomyocytes proliferation, reduces cardiac fibrosis as well as dilation, and inhibits apoptosis following ischemic stress such as myocardial infarction. Inactivation of PTEN exhibits a potentially beneficial therapeutic effects against cardiac diseases. In this review, we summarize various strategies for PTEN inactivation and highlight the roles of PTEN-less in regulating cardiomyocytes during cardiac development and stress responses.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"10 1","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2021-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39267370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12