Cell and Tissue Biology最新文献

{"title":"Surfactant Proteins SP-A and SP-D and Conventional Risk Factors for Chronic Noncommunicable Human Diseases","authors":"K. Yu. Nikolaev, O. S. Kharlamova, I. A. Kosarev, N. F. Dadashova, Y. K. Lapitskay","doi":"10.1134/s1990519x2370013x","DOIUrl":"https://doi.org/10.1134/s1990519x2370013x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Surfactant proteins SP-A and SP-D, members of the type C collagen-containing lectin family, are used as diagnostic and prognostic markers for many acute and chronic diseases of the bronchopulmonary system. The aim of the study was to assess the influence of conventional risk factors for chronic noncommunicable diseases on the level of surfactant proteins SP-A and SP-D through a system-structural analysis based on current publications from international databases and official WHO reports. Based on the results of the analytical review, it is concluded that widespread expression of SP-A and SP-D have been documented in numerous studies, and, although the lung remains the main site of the synthesis of surfactant protein, they can be expected to have a significant influence on the immune and inflammatory response in many organs and tissues. The authors note that a number of extrapulmonary effects of these proteins are known. However, many mechanisms of additional cellular effects of SP-A and SP-D outside the bronchopulmonary system still remain unexplored, which indicates the promise of further research in this area.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and Comparison of the Characteristics of Mesenchymal Stem Cells from Pulp of the Milk Teeth of Children of Different Sex","authors":"A. S. Musorina, V. I. Turilova, A. N. Shatrova, T. K. Yakovleva, G. G. Poljanskaya","doi":"10.1134/s1990519x24010061","DOIUrl":"https://doi.org/10.1134/s1990519x24010061","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Two new nonimmortalized fibroblast-like cell lineages (MSC-DP-1 and MSC-DP-2) isolated from the pulp of milk teeth of two 6-year-old children of different sex were obtained and characterized. The status of mesenchymal stem cells was confirmed by comparative analysis of a number of characteristics of these cells at early and late passages. The long-term cultivation revealed significant interstrain differences in growth characteristics and replicative senescence (RS). The MSC-DP-1 cells were characterized by a later entry into the MS active stage and more active proliferation compared to MSC-DP-2 cells. Karyotypic analysis showed that both lineages at early passages have a normal human diploid karyotype. Late (18th passage) in the stage of active RS, MSC-DP-2 cells retain the normal karyotype. MSC-DP-1 cells, which enter the active RS much later (at the 42nd passage), had an abnormal karyotype with a large the number of clonal and nonclonal chromosomal rearrangements. Both lineages showed a high proportion of cells carrying surface antigens characteristic of human MSCs—CD44, CD73, CD90, CD105, and HLA-ABC—and a low frequency of cells with CD34, CD45, and HLA-DR antigens. Cells of both lineages at an early passage demonstrated the ability to differentiate in the adipogenic, osteogenic, and chondrogenic directions. However, MSC-DP-2 cells showed a weaker differentiation capacity in the adipogenic direction than MSC-DP-1 cells. During RS, adipogenic differentiation in MSC-DP-1 cells is significantly weakened, while in MSC-DP-2 cells it disappeared. The activity of other differentiations did not change during RS. In general, the results obtained confirmed the MSC status of obtained lineages and revealed interlineage differences in the RS. However, the comparison with the previously obtained MSC-DP lineages did not indicate a gendered nature of the observed differences between these cells. Apparently, they are associated with the genetic characteristics of different donors.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Organization of the Rat Hippocampal Formation","authors":"C. M. Zimatkin, T. V. Klimut, A. V. Zaerko","doi":"10.1134/s1990519x24010115","DOIUrl":"https://doi.org/10.1134/s1990519x24010115","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The review examines historical aspects of hippocampal nomenclature. His anatomical study began with the ancient Greeks, who named the unusual-looking structure the “ram’s horn,” the cornu ammonis. The term “hippocampus” (Ancient Greek: ἱππόκαμπος, from ἵππος “horse” and κάμπος “sea monster” or “seahorse”) was first coined in the 16th century by the anatomist J.C. Arantius. The term “hippocampal formation” is currently applied to a group of cytoarchitectonically distinct adjacent regions, including, along with the hippocampus itself, the dentate gyrus, subiculum, presubiculum, parasubiculum, and entorhinal cortex. The rationale for including these six regions in the hippocampal formation group is that they are connected to each other by unique and largely unidirectional pathways. The review is devoted to the spatial, morphological, and cyto- and myeloarchitectonic organization of all parts of the rat hippocampal formation and the distinctive neuroanatomical characteristics of its parts. The comparative features of the structure of the hippocampal formation of rats, monkeys, and human beings are described. Although the volume of the hippocampus is approximately ten times larger in monkeys and 100 times larger in human beings than in rats, the basic architecture of the hippocampal formation is common, although there are some species differences. The relatively simple organization of the main cell layers, combined with the highly organized laminar distribution of hippocampal neuronal processes, facilitates its use as a model system in modern neurobiology.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of the Effect of Glycodelin on the Level of T-Regulatory Lymphocytes and Acute Phase Proteins in Wistar Rats during the Administration of Allogenic Bone Marrow Cells","authors":"S. A. Zamorina, M. S. Bochkova, V. P. Timganova, S. V. Uzhviyuk, K. Yu. Shardina, V. V. Vlasova, M. B. Rayev","doi":"10.1134/s1990519x24010103","DOIUrl":"https://doi.org/10.1134/s1990519x24010103","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The amniotic variant of glycodelin (GdA) has pronounced immunomodulatory properties, being involved in the formation of immune tolerance during pregnancy. The effect of GdA on the amount of T-regulatory lymphocytes (Treg) and the level of acute phase proteins (α-2-macroglobulin (α-2M), orosomucoid, C-reactive protein (CRP)) were studied during the administration of allogeneic red bone marrow (BM) cells to Wistar rats in a dynamic in vivo experiment. It was established that the administration of GdA against the background of the administration of allogeneic BM caused an increase in the portion of peripheral Tregs among CD4⁺ lymphocytes at the end of the experiment (on the 21st day) as compared with the group, which received BM. It was demonstrated that GdA decreased the level of CRP and α-2M, but increased the orosomucoid concentration in the serum of experimental animals at the beginning of the experiment (third day); however, normalization of the content of acute phase proteins to the level of intact animals was observed by the end of the experiment (21st day) in all groups of experimental animals. Thus, glycodelin is able to realize an immunosuppressive effect relative to the allogeneic cells through an increase in the level of Treg and orosomucoid, as well as a decrease in the concentration of CRP and α-2M.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism and Receptor Mechanisms of Niacin Action","authors":"S. E. Boronovskiy, V. S. Kopylova, Y. R. Nartsissov","doi":"10.1134/s1990519x23700025","DOIUrl":"https://doi.org/10.1134/s1990519x23700025","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The paper discusses the metabolism of niacin, also known as vitamin B3 or PP, and the mechanisms of its receptor-induced functions in the human body. Niacin exists as several molecular compounds that act as the nicotinamide coenzymes precursors. These coenzymes play a crucial role in metabolism, being electron donors or acceptors in redox reactions catalyzed by various enzymes. Maintaining the intracellular pool of niacin is vital not only for redox metabolism, but also for the NAD-dependent pathways functioning. However, pathophysiological situations and changes in enzyme activity may influence the need for different forms of niacin. In addition to indirect effects via nicotinamide coenzymes, it also has a number of direct effects, including antilipolytic, vasodilatory, and neuroprotective functions, which exact mechanisms have not been studied fully up to date. Overall, niacin plays a vital role in maintaining efficient cellular function, and further study of its effects on various physiological processes, including the gut microbiome and epigenetic regulation, may lead to new discoveries and treatments for various diseases.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"207 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Immunohistochemical Study of Matrix Metalloproteinase 2 and 9 in the Left Ventricle of the Heart of Preterm Born Rats in the Late Period of Ontogenesis","authors":"V. V. Ivanova, O. N. Serebryakova, A. V. Erokhina, A. D. Nikonova, I. V. Milto","doi":"10.1134/s1990519x24010036","DOIUrl":"https://doi.org/10.1134/s1990519x24010036","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Immunohistochemical detection of matrix metalloproteinase (MMP) 2 and 9 was carried out in the myocardium of the left ventricle of preterm born (21 and 21.5 days of pregnancy) and full-term (22 days of pregnancy) rats on days 42, 56, and 180 of the postnatal period of ontogenesis. The intensity of immunopositive staining was assessed semiquantitatively. Preterm birth leads to increased immunopositive reaction intensity to MMP-2 and MMP-9 in the wall of the left ventricle of the rat heart. An increase in the intensity of the positive reaction to MMP-2 in the left ventricle of the heart of rats is observed the earlier, the greater the degree of prematurity. The intensity of the positive reaction to MMP-9 in the left ventricle of the heart of male rats increases with increasing degree of prematurity. An increase in the intensity of the positive reaction to MMP-2 and MMP-9 in the wall of the left ventricle of female rats due to preterm birth is determined exclusively in animals born on the 21st day of pregnancy, that is, with a greater degree of prematurity.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of EA.hy926 Endothelial Cells Using Atomic Force and Scanning Ion Conductance Microscopy","authors":"S. N. Pleskova, N. A. Bezrukov, E. N. Gorshkova, S. Z. Bobyk, E. V. Lazarenko","doi":"10.1134/s1990519x24010073","DOIUrl":"https://doi.org/10.1134/s1990519x24010073","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A two-section analytical chamber was developed and tested for study of EA.hy926 endothelial cells culture in real time with high resolution. It has been shown that the method of scanning ion-conductance microscopy yields relevant results, since it ensures the absence of mechanical effects on cells, and allows to scan endothelial cells on membranes that ensures that cells are surrounded by a nutrient medium. The method made it possible not only to visualize changes in the cell surface during long-term scanning, but also to detect extracellular (microfilaments) and intracellular (nucleolus) structures. The creating of a rigidity map allowed it to be estimated that the mean rigidity of endothelial cell membranes was in the range from 357 to 796 Pa. After 240 min from the start of observation, the endothelial cells began to produce apoptotic bodies, wherein the rigidity of the cells gradually increased and the rigidity of the apoptotic bodies decreased.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial Cell Reprogramming as a Way to Revitalize Living Systems","authors":"M. A. Shorokhova","doi":"10.1134/s1990519x23700104","DOIUrl":"https://doi.org/10.1134/s1990519x23700104","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Aging and associated diseases are acute problems of modern biology and medicine. Although aging cannot currently be prevented, its impact on the lifespan and health of older adults can potentially be minimized through interventions aimed at returning cells to normal function. The constant search for ways to rejuvenate and improve the regenerative capacity of cells led to the discovery in 2016 of a partial reprogramming method based on short-term expression of reprogramming factors (Oct4, Sox2, Klf4, and c-Myc). As a result, the youthful epigenetic signature of aging cells is restored. The effectiveness of the method is shown as in the system in vitro and in vivo. The presented review discusses the main successes of partial reprogramming, as well as the problems and unresolved questions that researchers have encountered. Data on molecular changes during the process of partial reprogramming are discussed separately. The partial reprogramming method provides a wide range of opportunities for fundamental research on aging and rejuvenation. Further work in this direction may lead to the development of therapeutic strategies to alleviate age-related diseases and, thus, improve health and longevity.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of Neurodegenerative Changes in the CA1 Region of the Dorsal Hippocampus in Adult Rats with Prenatal Hyperhomocysteinemia","authors":"N. L. Tumanova, D. S. Vasiliev, N. M. Dubrovskaya","doi":"10.1134/s1990519x23700116","DOIUrl":"https://doi.org/10.1134/s1990519x23700116","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The work is devoted to the study of neurodegenerative changes in the ultrastructural organization of hippocampal CA1 in adult rats that have suffered prenatal hyperhomocysteinemia (pHHC). Electron microscopy in the neural networks of the CA1 region of the dorsal hippocampus in adult rats with pHHC, in contrast to control animals, revealed signs of pathological changes: degeneration of pyramidal neurons and destruction of the myelin sheath of axons, as well as destruction of the axial cylinders of basal and apical dendrites directed from a pyramidal layer of neurons in the direction of <i>tractus temporammonic</i> or Schaffer collaterals, respectively. In control animals, on the distal branches of dendrites in the layers of the <i>stratum oriens</i> and <i>stratum radiatum</i>, using the Golgi method, a dense network of varicose dendritic extensions was identified, providing an increase in the area of synaptic contacts. In rats that have undergone pHHC, significant destructive changes are found in these dendritic varicosities: destruction of mitochondrial cristae and the appearance of dilated cisterns. In adult rats with pHHC, it completely eliminates the preference for the smell of valerian, which is normally a physiologically significant stimulus, which indicates a negative effect of pHHC on the functioning of the olfactory analyzer, the activity of which is closely connected with the hippocampus. The obtained facts indicate the detrimental effect of homocysteine on the structure and interneuronal connections in the nervous tissue of the CA1 region of the dorsal hippocampus, as a morphological substrate for the integration of stimuli entering it.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Cytokine Profile of Myocardial Cells with Coronary Heart Disease and Ischemic Cardiomyopathy","authors":"A. I. Stelmashenko, S. L. Andreev, L. S. Litvinova, V. V. Malashchenko, N. M. Todosenko, N. D. Gazatova, I. A. Khlusov, V. M. Shipulin","doi":"10.1134/s1990519x24010097","DOIUrl":"https://doi.org/10.1134/s1990519x24010097","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this work, a comparative analysis of the myocardial cytokine profile was carried out in patients with coronary heart disease (CHD) and in patients with ischemic cardiomyopathy (ICM) against the background of CHD. The concentrations of 41 cytokines secreted by a 24-h tissue culture of the myocardium, intraoperatively collected from the right atrial (RA) appendage (control) and a peri-infarct zone (PZ) of the left ventricle (LV), were determined by the flow fluorimetry method using a multiplex test system. The aim of the work was to study in vitro the cytokine profile of myocardial cells to search for possible predictors of unfavorable outcomes of the surgical treatment of patients with CHD and ICM. The myocardial secretion of the pro-inflammatory molecules GM-CSF and IFN-γ increased significantly in patients with ICM against the background of CHD (up to 78–80 pg/g, <i>p</i> &lt; 0.05) as compared with zero values in the case of CHD. At the same time, a threefold decrease in the concentration of the Fractalkine 3 ligand (Flt-3L, fms-like tyrosine kinase 3 ligand) was observed. A decrease in the secretion of Flt-3L was noted namely in the PZ–LV as compare with the RA appendage. In addition, the concentrations of fibroblast growth factor-2 (FGF-2), platelet-derived growth factor AB/BB (PDGF-AB/BB), interleukins IL-15 and IL-4, as well as regulated upon activation, normal T cell expressed and secreted (RANTES/CCL5), sharply decreased in the tissue myocardial PZ–LV culture as compared with RA appendage. The detected changes are analyzed using correlation and regression analyses; possible predictors of the risk of surgical treatment of patients from two groups are suggested. Pro-inflammatory cytokines (IL-5, IL-6) and chemokines (Flt-3L, IL-8), as well as factors of angiogenesis (VEGF) and angiostasis (IP-10), are suggested to be considered as potential markers of unfavorable outcome of the surgical treatment of cardiovascular diseases.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}