An Immunohistochemical Study of Matrix Metalloproteinase 2 and 9 in the Left Ventricle of the Heart of Preterm Born Rats in the Late Period of Ontogenesis

Q4 Biochemistry, Genetics and Molecular Biology

Cell and Tissue Biology Pub Date : 2024-04-15 DOI:10.1134/s1990519x24010036

V. V. Ivanova, O. N. Serebryakova, A. V. Erokhina, A. D. Nikonova, I. V. Milto

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Abstract

Immunohistochemical detection of matrix metalloproteinase (MMP) 2 and 9 was carried out in the myocardium of the left ventricle of preterm born (21 and 21.5 days of pregnancy) and full-term (22 days of pregnancy) rats on days 42, 56, and 180 of the postnatal period of ontogenesis. The intensity of immunopositive staining was assessed semiquantitatively. Preterm birth leads to increased immunopositive reaction intensity to MMP-2 and MMP-9 in the wall of the left ventricle of the rat heart. An increase in the intensity of the positive reaction to MMP-2 in the left ventricle of the heart of rats is observed the earlier, the greater the degree of prematurity. The intensity of the positive reaction to MMP-9 in the left ventricle of the heart of male rats increases with increasing degree of prematurity. An increase in the intensity of the positive reaction to MMP-2 and MMP-9 in the wall of the left ventricle of female rats due to preterm birth is determined exclusively in animals born on the 21st day of pregnancy, that is, with a greater degree of prematurity.

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早产大鼠心脏发育后期左心室基质金属蛋白酶 2 和 9 的免疫组织化学研究

摘要在早产大鼠（妊娠21天和21.5天）和足月大鼠（妊娠22天）出生后第42天、56天和180天的左心室心肌中对基质金属蛋白酶（MMP）2和9进行了免疫组织化学检测。对免疫阳性染色的强度进行了半定量评估。早产会导致大鼠左心室壁上 MMP-2 和 MMP-9 的免疫阳性反应强度增加。早产程度越高，大鼠左心室中 MMP-2 的阳性反应强度越高。雄性大鼠心脏左心室对 MMP-9 的阳性反应强度随着早产程度的增加而增加。早产导致的雌性大鼠左心室壁 MMP-2 和 MMP-9 阳性反应强度的增加仅在妊娠第 21 天（即早产程度越高）出生的动物中出现。

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来源期刊

Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

0.80

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0.00%

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期刊介绍： The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.