S. N. Pleskova, N. A. Bezrukov, E. N. Gorshkova, S. Z. Bobyk, E. V. Lazarenko
{"title":"使用原子力显微镜和扫描离子传导显微镜对 EA.hy926 内皮细胞的研究","authors":"S. N. Pleskova, N. A. Bezrukov, E. N. Gorshkova, S. Z. Bobyk, E. V. Lazarenko","doi":"10.1134/s1990519x24010073","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A two-section analytical chamber was developed and tested for study of EA.hy926 endothelial cells culture in real time with high resolution. It has been shown that the method of scanning ion-conductance microscopy yields relevant results, since it ensures the absence of mechanical effects on cells, and allows to scan endothelial cells on membranes that ensures that cells are surrounded by a nutrient medium. The method made it possible not only to visualize changes in the cell surface during long-term scanning, but also to detect extracellular (microfilaments) and intracellular (nucleolus) structures. The creating of a rigidity map allowed it to be estimated that the mean rigidity of endothelial cell membranes was in the range from 357 to 796 Pa. After 240 min from the start of observation, the endothelial cells began to produce apoptotic bodies, wherein the rigidity of the cells gradually increased and the rigidity of the apoptotic bodies decreased.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"54 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Study of EA.hy926 Endothelial Cells Using Atomic Force and Scanning Ion Conductance Microscopy\",\"authors\":\"S. N. Pleskova, N. A. Bezrukov, E. N. Gorshkova, S. Z. Bobyk, E. V. Lazarenko\",\"doi\":\"10.1134/s1990519x24010073\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>A two-section analytical chamber was developed and tested for study of EA.hy926 endothelial cells culture in real time with high resolution. It has been shown that the method of scanning ion-conductance microscopy yields relevant results, since it ensures the absence of mechanical effects on cells, and allows to scan endothelial cells on membranes that ensures that cells are surrounded by a nutrient medium. The method made it possible not only to visualize changes in the cell surface during long-term scanning, but also to detect extracellular (microfilaments) and intracellular (nucleolus) structures. The creating of a rigidity map allowed it to be estimated that the mean rigidity of endothelial cell membranes was in the range from 357 to 796 Pa. After 240 min from the start of observation, the endothelial cells began to produce apoptotic bodies, wherein the rigidity of the cells gradually increased and the rigidity of the apoptotic bodies decreased.</p>\",\"PeriodicalId\":9705,\"journal\":{\"name\":\"Cell and Tissue Biology\",\"volume\":\"54 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell and Tissue Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1134/s1990519x24010073\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x24010073","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
A Study of EA.hy926 Endothelial Cells Using Atomic Force and Scanning Ion Conductance Microscopy
Abstract
A two-section analytical chamber was developed and tested for study of EA.hy926 endothelial cells culture in real time with high resolution. It has been shown that the method of scanning ion-conductance microscopy yields relevant results, since it ensures the absence of mechanical effects on cells, and allows to scan endothelial cells on membranes that ensures that cells are surrounded by a nutrient medium. The method made it possible not only to visualize changes in the cell surface during long-term scanning, but also to detect extracellular (microfilaments) and intracellular (nucleolus) structures. The creating of a rigidity map allowed it to be estimated that the mean rigidity of endothelial cell membranes was in the range from 357 to 796 Pa. After 240 min from the start of observation, the endothelial cells began to produce apoptotic bodies, wherein the rigidity of the cells gradually increased and the rigidity of the apoptotic bodies decreased.
期刊介绍：
The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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