Cell and Tissue Biology最新文献_第3页

α-Actinin-4-Dependent Regulation of DNA Break Repair Is Not Dependent on NF-kB Activity α-Actinin-4对DNA断裂修复的调控不依赖于NF-kB的活性

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700251

D. V. Krieger, G. V. Vasilyeva, E. V. Lomert, D. G. Tentler

{"title":"α-Actinin-4-Dependent Regulation of DNA Break Repair Is Not Dependent on NF-kB Activity","authors":"D. V. Krieger, G. V. Vasilyeva, E. V. Lomert, D. G. Tentler","doi":"10.1134/s1990519x24700251","DOIUrl":"https://doi.org/10.1134/s1990519x24700251","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>α-Actinin-4 is an actin-binding protein involved in a wide range of cellular processes. Along with actin and other proteins of the actin cytoskeleton, α-actinin-4 is found not only in the cytoplasm, but also in the nucleus of various types of cells. As a nuclear protein, it takes part in regulating the activity of some transcription factors. In particular, it can regulate the activity of the NF-kB factor, which largely determines the resistance of cancer cells to apoptosis and anticancer therapy. Our previous studies revealed that α-actinin-4 can influence the resistance of cancer cells to topoisomerase II inhibitors and determine the efficiency of DNA double-strand break repair by regulating the assembly of HRR and NHEJ protein complexes. In this work, we tried to answer the question of how α-actinin-4 is involved in the regulation of the double-stranded DNA breaks repair under genotoxic stress. Our results indicate that the effect of α-actinin-4 on the repair process in H1299 non-small-cell lung-cancer cells does not depend on the activity of the transcription factor NF-kB. We found that, in the nucleus of H1299 cells, α-actinin-4 is localized not only in the nucleoplasm, but also shows close association with chromatin.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Kidney Damage Development in Patients with New Coronavirus Infection: Literature Review 新型冠状病毒感染患者肾损伤的发生机制：文献综述

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700196

E. V. Utkina, V. V. Novakovskaya, M. V. Egorova, N. V. Fomina, L. D. Chesnokova

{"title":"Mechanisms of Kidney Damage Development in Patients with New Coronavirus Infection: Literature Review","authors":"E. V. Utkina, V. V. Novakovskaya, M. V. Egorova, N. V. Fomina, L. D. Chesnokova","doi":"10.1134/s1990519x24700196","DOIUrl":"https://doi.org/10.1134/s1990519x24700196","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Every fourth person in the world currently has kidney problems to one or another degree. It is known that the novel coronavirus infection (COVID-19) is primarily a respiratory disease, but the kidney is a target organ. The coronavirus is tropic to kidney tissue due to the presence in the organ of RNA of angiotensin-converting enzyme type 2 and transmembrane serine protease 2, which is considered to be a target of this virus. The presence of renal failure in any stage is an independent unfavorable risk factor for contracting coronavirus and leads to a high frequency of hospitalization and mortality rate. Kidney failure is caused by various pathogenetic mechanisms: the direct cytopathic effect of the virus on their structures (podocytes, mesangial cells in the renal corpuscle, capillary endothelium in the glomerulus, epithelial cells in the proximal tubules), cytokine storm, damage to the renin–angiotensin–aldosterone system, and immunothrombosis. In many patients with confirmed coronavirus infection, from the first days of the disease, laboratory tests show significant changes in urine analysis (hematuria, proteinuria) and increased level of creatinine in the blood serum. The development of acute kidney injury is a main mortality risk factor. More research is needed into the exact effects of SARS-CoV-2 on the kidneys. Understanding the main pathogenetic pathways of their damage in COVID-19 is necessary to develop strategies and effective treatments.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of the Expression of NRF2 Transcription Factor Mediated by miR-155 Causes a Decrease in the Viability of Melanoma Cells Regardless of Redox Status miR-155 介导的 NRF2 转录因子的表达抑制会降低黑色素瘤细胞的活力，与氧化还原状态无关

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x2470024x

V. A. Kutsenko, D. A. Dashkova, T. G. Ruksha

引用次数: 0

The Role of Autophagy and Macrophage Polarization in the Process of Chronic Inflammation and Regeneration 自噬和巨噬细胞极化在慢性炎症和再生过程中的作用

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700184

S. G. Zubova, A. V. Morshneva

{"title":"The Role of Autophagy and Macrophage Polarization in the Process of Chronic Inflammation and Regeneration","authors":"S. G. Zubova, A. V. Morshneva","doi":"10.1134/s1990519x24700184","DOIUrl":"https://doi.org/10.1134/s1990519x24700184","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Many serious illnesses, including diabetes, obesity, osteoporosis, and neurodegenerative diseases, are caused by chronic inflammation that develops in fat tissue, bones, or the brain. This inflammation occurs due to a shift in macrophage (microglia) polarization toward a proinflammatory M1 phenotype. It has now been proven that macrophage polarization is determined by the intracellular level of autophagy in the macrophage. By modulating autophagy, it is possible to cause a switch in macrophage activity towards M1 or M2. Summarizing the material accumulated in the literature, we believe that activation of autophagy reprograms the macrophage towards M2, replacing its protein content and receptor apparatus, and activate a another type of metabolism. The term “reprogramming” is most appropriate for this process, since it is followed by a change in the functional activity of the macrophage, namely a switch from cytotoxic proinflammatory activity to anti-inflammatory (regenerative) activity. Modulation of autophagy may be an approach to the treatment of cancer, neurodegenerative disorders, osteoporosis, diabetes and other serious diseases.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Biological Activity and Therapeutic Potential of Curcuma longa: Literature Review 姜黄的生物活性和治疗潜力：文献综述

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700202

A. A. Zaporozhchenko, M. A. Subotyalov

引用次数: 0

Decellularized Prostate for Cancer Studies 用于癌症研究的脱细胞前列腺

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700160

Mozafar Khazaei, Mohammad Rasool Khazaei, Elham Ghanbari, Leila Rezakhania

{"title":"Decellularized Prostate for Cancer Studies","authors":"Mozafar Khazaei, Mohammad Rasool Khazaei, Elham Ghanbari, Leila Rezakhania","doi":"10.1134/s1990519x24700160","DOIUrl":"https://doi.org/10.1134/s1990519x24700160","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Regenerative medicine researchers are interested in extracellular matrix (ECM) investigations around fabricating scaffolds that mimic the biological environment. These structures can be utilized in different disease and drug screenings as three-dimensional (3D) models. This study aimed to decellularization of rat prostate by different methods to reach a suitable scaffold and introduce it in cancer studies. In this experimental study, rat prostates were decellularized by 1% sodium dodecyl sulfate (SDS) and 1% sodium deoxycholate (SD) methods. Decellularized prostate matrix (DPM) was stained using hematoxylin-eosin (H&E) and Masson’s trichrome (MT) techniques to determine the presence of the nucleus and collagen. A scanning electron microscope (SEM) was used to examine the morphology and cell attachment to the DPM. By culturing LNcap cells on the DPM, recellularization was studied. Both decellularization methods (1% SDS and 1% SD) completely removed the cells from the DPM. The tissue structure has been significantly preserved, which is confirmed by the staining methods and SEM images. The preservation of a large amount of collagen content was established through MT staining and using the kit. Cell adhesion in the DPM was reported by SEM. No toxicity or hemolysis was observed in the DPM. Migration of cancer cells into the DPM was seen. Prostate decellularization with SDS and SD, in addition to cell removal, can maintain the ECM structure to a large extent without having cytotoxic and hemolysis effects.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short Tandem Repeat Profile for Authentication of Immortal Murine Cancer Cell Line MH-22A 用于鉴定不死小鼠癌症细胞系 MH-22A 的短串联重复序列图

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700214

Ralf Weiskirchen, Stefanie Kankel, Thomas Liehr

引用次数: 0

Vimentin in Kolmer Cells of Spontaneously Hypertensive Rats 自发性高血压大鼠 Kolmer 细胞中的波形蛋白

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700275

D. E. Korzhevskii, V. A. Razenkova, O. V. Kirik

引用次数: 0

Role of Rho Family Small GTPases in the Regulation of Normal and Pathological Processes Rho 家族小 GTP 酶在正常和病理过程调控中的作用

Cell and Tissue Biology Pub Date : 2024-06-03 DOI: 10.1134/s1990519x24700159

D. E. Bobkov, A. V. Lukacheva, A. I. Gorb, G. G. Poljanskaya