Short Tandem Repeat Profile for Authentication of Immortal Murine Cancer Cell Line MH-22A

Q4 Biochemistry, Genetics and Molecular Biology

Cell and Tissue Biology Pub Date : 2024-06-03 DOI:10.1134/s1990519x24700214

Ralf Weiskirchen, Stefanie Kankel, Thomas Liehr

引用次数: 0

Abstract

The murine cell line MH-22A was originally established at least 50 years ago from a non-metastasizing tumor induced by 3-methylcholanthrene in C3HA mice and further adapted for in vitro growth. MH-22A cells grow predominantly epithelioid and they lead to the formation of transplantable tumors when injected subcutaneously into mice. In silico translation of genome data of this nearly triploid cell line to the human genome has recently characterized MH-22A as a suitable model for hepatoblastoma or early hepatocellular carcinoma. Here we established a multi-loci short tandem repeat signature for this cell line that allows quick and reliable cell line authentication.

Abstract Image

查看原文本刊更多论文

用于鉴定不死小鼠癌症细胞系 MH-22A 的短串联重复序列图

摘要小鼠细胞系 MH-22A 最初是在至少 50 年前从 C3HA 小鼠体内由 3-甲基胆蒽诱发的非转移性肿瘤中建立的，后经进一步改造用于体外生长。MH-22A 细胞主要呈上皮样生长，向小鼠皮下注射后可形成可移植肿瘤。最近，将这一近乎三倍体细胞系的基因组数据转化为人类基因组的硅学研究表明，MH-22A 是肝母细胞瘤或早期肝细胞癌的合适模型。在这里，我们为该细胞系建立了一个多基因短串联重复特征，可以快速可靠地鉴定细胞系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.