Mozafar Khazaei, Mohammad Rasool Khazaei, Elham Ghanbari, Leila Rezakhania
{"title":"Decellularized Prostate for Cancer Studies","authors":"Mozafar Khazaei, Mohammad Rasool Khazaei, Elham Ghanbari, Leila Rezakhania","doi":"10.1134/s1990519x24700160","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Regenerative medicine researchers are interested in extracellular matrix (ECM) investigations around fabricating scaffolds that mimic the biological environment. These structures can be utilized in different disease and drug screenings as three-dimensional (3D) models. This study aimed to decellularization of rat prostate by different methods to reach a suitable scaffold and introduce it in cancer studies. In this experimental study, rat prostates were decellularized by 1% sodium dodecyl sulfate (SDS) and 1% sodium deoxycholate (SD) methods. Decellularized prostate matrix (DPM) was stained using hematoxylin-eosin (H&E) and Masson’s trichrome (MT) techniques to determine the presence of the nucleus and collagen. A scanning electron microscope (SEM) was used to examine the morphology and cell attachment to the DPM. By culturing LNcap cells on the DPM, recellularization was studied. Both decellularization methods (1% SDS and 1% SD) completely removed the cells from the DPM. The tissue structure has been significantly preserved, which is confirmed by the staining methods and SEM images. The preservation of a large amount of collagen content was established through MT staining and using the kit. Cell adhesion in the DPM was reported by SEM. No toxicity or hemolysis was observed in the DPM. Migration of cancer cells into the DPM was seen. Prostate decellularization with SDS and SD, in addition to cell removal, can maintain the ECM structure to a large extent without having cytotoxic and hemolysis effects.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"51 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x24700160","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Regenerative medicine researchers are interested in extracellular matrix (ECM) investigations around fabricating scaffolds that mimic the biological environment. These structures can be utilized in different disease and drug screenings as three-dimensional (3D) models. This study aimed to decellularization of rat prostate by different methods to reach a suitable scaffold and introduce it in cancer studies. In this experimental study, rat prostates were decellularized by 1% sodium dodecyl sulfate (SDS) and 1% sodium deoxycholate (SD) methods. Decellularized prostate matrix (DPM) was stained using hematoxylin-eosin (H&E) and Masson’s trichrome (MT) techniques to determine the presence of the nucleus and collagen. A scanning electron microscope (SEM) was used to examine the morphology and cell attachment to the DPM. By culturing LNcap cells on the DPM, recellularization was studied. Both decellularization methods (1% SDS and 1% SD) completely removed the cells from the DPM. The tissue structure has been significantly preserved, which is confirmed by the staining methods and SEM images. The preservation of a large amount of collagen content was established through MT staining and using the kit. Cell adhesion in the DPM was reported by SEM. No toxicity or hemolysis was observed in the DPM. Migration of cancer cells into the DPM was seen. Prostate decellularization with SDS and SD, in addition to cell removal, can maintain the ECM structure to a large extent without having cytotoxic and hemolysis effects.
期刊介绍：
The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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