Cell and Tissue Biology最新文献_第5页

Antioxidant Properties of Brown Algae in 3D Model for Colorectal Cancer 褐藻在结直肠癌三维模型中的抗氧化特性

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700128

Mozafar Khazaei, Saeed Seyfi, Mohammad Rasool Khazaei, Azam Bozorgi Zarrini, Leila Rezakhani

{"title":"Antioxidant Properties of Brown Algae in 3D Model for Colorectal Cancer","authors":"Mozafar Khazaei, Saeed Seyfi, Mohammad Rasool Khazaei, Azam Bozorgi Zarrini, Leila Rezakhani","doi":"10.1134/s1990519x23700128","DOIUrl":"https://doi.org/10.1134/s1990519x23700128","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A potential scientific viewpoint is provided by three-dimensional (3D) cancer models, which have as their main objective bridging the differences between two-dimensional (2D) models, animal models, and clinical research. We used a tissue engineering approach to engineer colorectal cancer (CT26 cell line) with a decellularized sheep colon to create a 3D biologic model. A decellularized colon matrix (DCM) was prepared with 1% sodium dodecyl sulfate (SDS) and its DNA content, biocompatibility, hemocompatibility, histology, cell adhesion, and tissue ultrastructure were characterized. Brown algae exhibit various biological activities, including anticancer activity, connected to the impact of carotenoids, glyceroglycolipids, fucoidan sulfate polysaccharides, or iodine compounds. In both a 2D culture (culture plate) and 3D (DCM) model, CT26 cells were treated with brown algae extract and doxorubicin (DOX), and their viability, total antioxidant capacity (TAC), and nitric oxide (NO) secretion were assessed. DCM retains a significant amount of its biological and structural characteristics. In both models, cell survival was decreased. Groups that received algae demonstrated antioxidant activity. A decrease in NO secretion was demonstrated in cancer cells that had been treated with algae and DOX, the 3D model’s drug sensitivity was lower than the 2D model. Due to the biological activity of the extracellular matrix, the use of decellularized scaffolds in the construction of cancer models can thus be a potent tool for future research and drug screens.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized Extracellular Matrix Slows Down Premature Senescence of Human Endometrial Mesenchymal Stromal Cells 脱细胞细胞外基质可延缓人类子宫内膜间充质基质细胞的过早衰老

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700037

E. B. Burova, I. E. Perevoznikov, R. E. Ushakov

{"title":"Decellularized Extracellular Matrix Slows Down Premature Senescence of Human Endometrial Mesenchymal Stromal Cells","authors":"E. B. Burova, I. E. Perevoznikov, R. E. Ushakov","doi":"10.1134/s1990519x23700037","DOIUrl":"https://doi.org/10.1134/s1990519x23700037","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The extracellular matrix (ECM), the main component of the extracellular space, mediates signal transmission between cells and controls their key functions: proliferation, differentiation, and migration. The relevance of studying ECM research is stipulated to the wide range of its biological properties, which can be used in regenerative medicine and bioengineering. Particular interest is presented the study of the regulatory activity on various cellular functions of cell-derived decellularized ECM (dECM). In this work, we tested the hypothesis about the modulating effect of dECM deposited by young MSC from Wharton’s jelly on the aging phenotype of endometrial human multipotent mesenchymal stromal cells (eMSCs), which the cells acquired in response to oxidative stress. This aspect of ECM functioning in the context of eMSCs has not yet been considered. A comparative study of H<sub>2</sub>O<sub>2</sub>-induced senescence of eMSCs cultured on dECM and on plastic for a long time showed a significant change in the hallmarks of aging in the cell population maintained on dECM. Taken together, the results obtained suggest that the dECM is able to partially reverse (retard) premature senescence of eMSCs in response to oxidative stress, as well as expanding the understanding of the ECM as a regulator of the functional activity of cells.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of the Synthetic Polycation Poly-2-Dimethylaminoethyl Methacrylate on the Biological Activity of Resident and Nonresident Cells of Mammals 合成聚阳离子聚-2-二甲基氨基乙基甲基丙烯酸酯对哺乳动物常驻细胞和非常驻细胞生物活性的影响

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x24010024

V. P. Ivanova, L. L. Alekseenko, O. V. Nazarova, I. V. Mindukshev

{"title":"The Influence of the Synthetic Polycation Poly-2-Dimethylaminoethyl Methacrylate on the Biological Activity of Resident and Nonresident Cells of Mammals","authors":"V. P. Ivanova, L. L. Alekseenko, O. V. Nazarova, I. V. Mindukshev","doi":"10.1134/s1990519x24010024","DOIUrl":"https://doi.org/10.1134/s1990519x24010024","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Cationic polymers are positively charged high-molecular compounds that have N-containing functional groups, such as primary, secondary, and tertiary amine groups, quaternary ammonium groups, etc. In this work, we studied the effect of the synthetic polycation poly-2-dimethylaminoethyl methacrylate (PDMAEM) on the biological activity of Chinese hamster fibroblasts and human erythrocytes. Analysis of the effect of the polycation on cell adhesion was carried out using a fibroblast culture. Culture plastic, treated or untreated with polycation, was used as a substrate. Adsorption of the polycation on the polystyrene surface did not lead to a change in the adhesive ability of fibroblasts. Pretreatment of fibroblasts with PDMAEM in low concentrations (0.1 and 1 μg/mL) did not affect the adhesive properties of cells seeded on untreated plastic. At concentrations of 10 and 100 μg/mL, PDMAEM inhibited the attachment of fibroblasts to this substrate. A relationship has been determined between the suppression of cell adhesion under the influence of the polymer and its toxic effect on the viability of fibroblasts. Treatment of human erythrocytes with the polycation at concentrations of 10 and 100 μg/mL resulted in cell damage and release of hemoglobin from erythrocytes. At low doses, the polycation had practically no effect on the processes of hemolysis of erythrocytes. It was shown that PDMAEM caused morphological changes in erythrocytes and their aggregation. The toxic effect of the polycation on human erythrocytes generally coincided with that for animal fibroblasts. Possible cellular targets that may be affected by the studied polycation are discussed.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Features of the Wall of the Ascending Aorta of Premature Rats 早产大鼠升主动脉壁的结构特征

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700098

O. N. Serebryakova, V. V. Ivanova, I. V. Milto

引用次数: 0

Gap Junction Protein Connexin-43 in a Rat Dorsal Root Ganglion 大鼠背根神经节中的缝隙连接蛋白 Connexin-43

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700049

E. A. Kolos, D. E. Korzhevsky

{"title":"Gap Junction Protein Connexin-43 in a Rat Dorsal Root Ganglion","authors":"E. A. Kolos, D. E. Korzhevsky","doi":"10.1134/s1990519x23700049","DOIUrl":"https://doi.org/10.1134/s1990519x23700049","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study aimed at investigating the dynamics of the distribution and localization of the gap junction protein connexin-43 (Cx43) in rat dorsal root ganglion (DRG) cells at different stages of postnatal ontogenesis to assess morphological signs of age-related changes in intercellular interactions. The work was performed on Wistar rats aged 4 and 18 months using immunohistochemical methods. Glial cells were detected using antibodies to glutamine synthetase, and Iba-1 marker was used for macrophages. It has been established that connexin-43-containing structures are identified predominantly in satellite glial cells of young and aging animals. Sensory neurons, as well as DRG macrophages, of the examined age groups do not contain connexin-43. When analyzing age-related changes in intercellular contacts in the DRG of rats, it was found that areas enriched with connexin-43, corresponding to plaques of protein channels that ensure metabolic interaction of satellite glial cells in the dorsal root ganglia, became more numerous with age. This fact may indicate activation of interaction between glial cells in the sensory ganglia during rat aging.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"207 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erythropoietin: Functions and Therapeutic Potential 促红细胞生成素：功能和治疗潜力

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x2401005x

A. P. Lykov

{"title":"Erythropoietin: Functions and Therapeutic Potential","authors":"A. P. Lykov","doi":"10.1134/s1990519x2401005x","DOIUrl":"https://doi.org/10.1134/s1990519x2401005x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Erythropoietin (EPO) exerts its effect on erythroid cells through interaction with the EPO receptor (EPOR), the so-called “canonical pathway,” and through a complex consisting of EPOR and the common cytokine receptor β subunit (CD131)—a noncanonical pathway for non-hematopoietic cells of the bodies of human beings and animals. The effect of EPO is realized through the initiation of a signaling cascade, which begins with phosphorylation of Janus kinase 2 (JAK2) and extends with the involvement of phosphatidylinositol 3 kinase B (PI3K), Ras-mitogen-activated protein kinase (MAPK), or signal transducers and activators of transcription (STAT). EPO exerts a direct cytoprotective effect through increased expression of CD131 with subsequent antiapoptotic and anti-inflammatory effects in target cells. In addition to its use in the treatment of anemia, EPO is increasingly used in the correction of inflammatory and degenerative processes in both experimental and clinical cell-mediated studies. EPO promotes the engraftment of stem cells and differentiation of mesenchymal stem cells in the connective tissue direction, suppressing the inflammatory response and apoptosis of cells in the lesion. The article includes literature data regarding EPO and its clinical use in inflammatory–degenerative processes based on eLibrary and National Center for Biotechnology Information data from 1998 to 2022.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Mevalonate, Zoledronate, and BCG Induction on the Monocyte/Macrophage Phenotype 甲羟戊酸、唑来膦酸和卡介苗诱导对单核细胞/巨噬细胞表型的影响

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700050

A. P. Lykov, S. N. Belogorodtsev, E. K. Nemkova, A. Vetlugina, T. M. Terekhova, J. Sh. Schwartz

{"title":"The Effect of Mevalonate, Zoledronate, and BCG Induction on the Monocyte/Macrophage Phenotype","authors":"A. P. Lykov, S. N. Belogorodtsev, E. K. Nemkova, A. Vetlugina, T. M. Terekhova, J. Sh. Schwartz","doi":"10.1134/s1990519x23700050","DOIUrl":"https://doi.org/10.1134/s1990519x23700050","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Innate immune cells, mainly monocytes/macrophages, upon their first encounter with a pathogen, form long-term nonspecific immunological memory, so-called “trained immunity.” In its formation, an important role is played by metabolites of the mevalonate pathway. The purpose of the study was to investigate the effect of mevalonate pathway modulators, mevalonate and zoledronate, on the formation of trained immunity in human and animal monocytes/macrophages. Human monocyte-like cells THP-1 and U-937 and peritoneal macrophages from BALB/c mice were used. Trained immunity was induced <i>in vitro</i> by incubating THP-1 and U-937 cells with inactivated mycobacteria of the bacillus Calmette–Guérin (BCG) vaccine strain for 24 and 72 h and <i>in vivo</i> by intraperitoneal injection of BCG to BALB/c mice and isolation of peritoneal macrophages on the seventh day after infection (lag phase). Cell hyperreactivity was assessed by the response to a second stimulus with bacterial lipopolysaccharide (LPS) and mevalanate or zoledranate in the presence or absence of LPS. The level of lactate, cytokines (IL-1β, TNF-α, IL-10), nitric oxide and glucose was evaluated in conditioned media from cells. It was found that monocyte-like THP-1 and U-937 cells respond differently as concerns the production of cytokines and lactate and the consumption of glucose to the BCG stimulus with or without the lag phase. Mevalonate and zoledronate alone or in combination with LPS also differentially stimulate cytokine secretion. The presence of the lag phase for human monocyte-like cells is essential for the level of cytokine production and glucose consumption. Peritoneal macrophages have been shown to increase the release of proinflammatory cytokines in response to LPS, mevalonate, and zoledronate. Mevalonate and zoledronate induce trained immunity in monocytes/macrophages.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organization and Role of Bacterial SMC, MukBEF, MksBEF, Wadjet, and RecN Complexes 细菌 SMC、MukBEF、MksBEF、Wadjet 和 RecN 复合物的组织和作用

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x23700074

N. E. Morozova, A. S. Potysyeva, A. D. Vedyaykin

引用次数: 0

The Influence of Perinatal Hypoxia (Asphyxia) on the Distribution of the α1 Subunit of the GABAA Receptor in the Neocortex of Newborn Rats 围产期缺氧（窒息）对新生大鼠神经皮层中 GABAA 受体 α1 亚基分布的影响

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x24010048

L. I. Khozhay

{"title":"The Influence of Perinatal Hypoxia (Asphyxia) on the Distribution of the α1 Subunit of the GABAA Receptor in the Neocortex of Newborn Rats","authors":"L. I. Khozhay","doi":"10.1134/s1990519x24010048","DOIUrl":"https://doi.org/10.1134/s1990519x24010048","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The purpose of this work was to study the distribution of the α1 subunit of the GABA<sub>A</sub> receptor in the layers of the neocortex of rats in the neonatal period after exposure to hypoxia. The effect of hypoxia on the brain of newborn rats was carried out on the second neonatal day for 1 h at an oxygen content in the respiratory mixture of 7.8%. To identify the α1 subunit of GABA<sub>A</sub> receptor, immunohistochemical reaction was used. Protein content was assessed by the density of immunostaining of the reaction product in the cytoplasm and processes of neurons. We studied the somatosensory area of the neocortex at the fifth and tenth neonatal stages (P5, P10). It has been established that, in the early stages of the neonatal period, a significant population of young neurons is present in the neocortex, in the cytoplasm of which the α1 subunit, which is part of the GABA<sub>A</sub> receptor, is immunohistochemically detected. By the end of the neonatal period, in control animals, the density of staining of the immunohistochemical product of GABA<sub>A</sub> in the layers of the neocortex increases significantly. Exposure to perinatal hypoxia causes a reduction in the number of neurons containing the α1 subunit of the GABA<sub>A</sub> receptor and a significant decrease in the density of immunohistochemical staining in all layers of the neocortex.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Intensity of Degradation and Synthesis of the Dermal Intercellular Matrix in Rats after Local Cold Injury against the Background of Metabolic Syndrome 代谢综合征背景下局部冷损伤后大鼠真皮细胞间基质的降解和合成强度

Cell and Tissue Biology Pub Date : 2024-04-15 DOI: 10.1134/s1990519x24010085

N. A. Shutskiy, L. N. Gorbatova, S. L. Kashutin, N. S. Kholopov, L. L. Shagrov, A. L. Zashikhin

{"title":"The Intensity of Degradation and Synthesis of the Dermal Intercellular Matrix in Rats after Local Cold Injury against the Background of Metabolic Syndrome","authors":"N. A. Shutskiy, L. N. Gorbatova, S. L. Kashutin, N. S. Kholopov, L. L. Shagrov, A. L. Zashikhin","doi":"10.1134/s1990519x24010085","DOIUrl":"https://doi.org/10.1134/s1990519x24010085","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Research into the processes of tissue regeneration in the postcold period is currently in demand in connection with the development of the Arctic, which is characterized by extremely low temperatures. Metabolic or hormonal abnormalities based on insulin resistance and compensatory hyperinsulinemia, which can promote cardiovascular diseases and, consequently, lead to microcirculatory disorders, are aggravating factors that increase the consequences of cold injuries. In this work, we studied the processes of degradation and synthesis of the dermal intercellular matrix, as well as the concentration of sialic acids in the blood serum after local third-degree cold injury. It was revealed that the regenerative process after local cold damage began on the third day and was characterized by increased content of dermal collagen and thickness of collagen fibers. Regeneration against the background of metabolic syndrome was accompanied by a slowdown in tissue repair processes. The recovery process was accompanied with changed content of sialic acids in the blood serum. A distinctive feature of the dynamics of this indicator was enhanced concentration of sialic acids with metabolic disorders on the seventh day and a slow slight decrease throughout the experiment, which reached control values by the 21st day.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0